Amino acid dipepetide frequency for Gordonia phage OhMyWard

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.713AlaAla: 8.713 ± 1.477
0.472AlaCys: 0.472 ± 0.136
5.407AlaAsp: 5.407 ± 0.462
6.194AlaGlu: 6.194 ± 0.69
3.727AlaPhe: 3.727 ± 0.406
7.454AlaGly: 7.454 ± 1.136
1.89AlaHis: 1.89 ± 0.339
5.407AlaIle: 5.407 ± 0.817
5.931AlaLys: 5.931 ± 0.651
6.876AlaLeu: 6.876 ± 0.903
3.149AlaMet: 3.149 ± 0.444
3.727AlaAsn: 3.727 ± 0.516
1.995AlaPro: 1.995 ± 0.293
3.097AlaGln: 3.097 ± 0.303
4.199AlaArg: 4.199 ± 0.504
4.514AlaSer: 4.514 ± 0.505
4.882AlaThr: 4.882 ± 0.538
6.929AlaVal: 6.929 ± 0.737
0.84AlaTrp: 0.84 ± 0.226
2.572AlaTyr: 2.572 ± 0.33
0.0AlaXaa: 0.0 ± 0.0
Cys
0.42CysAla: 0.42 ± 0.213
0.105CysCys: 0.105 ± 0.065
0.42CysAsp: 0.42 ± 0.15
0.262CysGlu: 0.262 ± 0.124
0.052CysPhe: 0.052 ± 0.054
0.42CysGly: 0.42 ± 0.151
0.105CysHis: 0.105 ± 0.087
0.42CysIle: 0.42 ± 0.147
0.157CysLys: 0.157 ± 0.099
0.525CysLeu: 0.525 ± 0.16
0.052CysMet: 0.052 ± 0.049
0.21CysAsn: 0.21 ± 0.102
0.315CysPro: 0.315 ± 0.144
0.21CysGln: 0.21 ± 0.115
0.315CysArg: 0.315 ± 0.136
0.577CysSer: 0.577 ± 0.145
0.315CysThr: 0.315 ± 0.194
0.472CysVal: 0.472 ± 0.156
0.21CysTrp: 0.21 ± 0.099
0.21CysTyr: 0.21 ± 0.101
0.0CysXaa: 0.0 ± 0.0
Asp
5.617AspAla: 5.617 ± 0.601
0.21AspCys: 0.21 ± 0.127
3.727AspAsp: 3.727 ± 0.707
5.197AspGlu: 5.197 ± 0.558
3.097AspPhe: 3.097 ± 0.405
4.934AspGly: 4.934 ± 0.538
0.997AspHis: 0.997 ± 0.217
3.517AspIle: 3.517 ± 0.419
3.359AspLys: 3.359 ± 0.429
4.777AspLeu: 4.777 ± 0.441
2.52AspMet: 2.52 ± 0.388
2.834AspAsn: 2.834 ± 0.298
3.202AspPro: 3.202 ± 0.419
2.467AspGln: 2.467 ± 0.37
2.625AspArg: 2.625 ± 0.337
3.569AspSer: 3.569 ± 0.371
3.464AspThr: 3.464 ± 0.441
3.989AspVal: 3.989 ± 0.459
0.892AspTrp: 0.892 ± 0.209
1.575AspTyr: 1.575 ± 0.276
0.0AspXaa: 0.0 ± 0.0
Glu
7.086GluAla: 7.086 ± 0.669
0.367GluCys: 0.367 ± 0.16
4.199GluAsp: 4.199 ± 0.444
5.669GluGlu: 5.669 ± 0.704
2.047GluPhe: 2.047 ± 0.381
5.564GluGly: 5.564 ± 0.494
1.26GluHis: 1.26 ± 0.306
4.042GluIle: 4.042 ± 0.391
4.987GluLys: 4.987 ± 0.649
5.826GluLeu: 5.826 ± 0.46
2.152GluMet: 2.152 ± 0.352
2.625GluAsn: 2.625 ± 0.403
2.415GluPro: 2.415 ± 0.41
3.307GluGln: 3.307 ± 0.414
2.677GluArg: 2.677 ± 0.435
2.887GluSer: 2.887 ± 0.468
3.149GluThr: 3.149 ± 0.35
4.619GluVal: 4.619 ± 0.429
1.365GluTrp: 1.365 ± 0.277
2.572GluTyr: 2.572 ± 0.379
0.0GluXaa: 0.0 ± 0.0
Phe
2.834PheAla: 2.834 ± 0.498
0.315PheCys: 0.315 ± 0.126
3.464PheAsp: 3.464 ± 0.427
3.097PheGlu: 3.097 ± 0.472
1.102PhePhe: 1.102 ± 0.254
2.73PheGly: 2.73 ± 0.377
0.472PheHis: 0.472 ± 0.151
1.732PheIle: 1.732 ± 0.202
1.417PheLys: 1.417 ± 0.298
2.467PheLeu: 2.467 ± 0.417
1.522PheMet: 1.522 ± 0.281
1.627PheAsn: 1.627 ± 0.244
1.47PhePro: 1.47 ± 0.317
0.997PheGln: 0.997 ± 0.218
1.995PheArg: 1.995 ± 0.315
2.362PheSer: 2.362 ± 0.355
1.785PheThr: 1.785 ± 0.308
2.52PheVal: 2.52 ± 0.398
0.367PheTrp: 0.367 ± 0.114
1.05PheTyr: 1.05 ± 0.248
0.0PheXaa: 0.0 ± 0.0
Gly
6.194GlyAla: 6.194 ± 0.93
0.525GlyCys: 0.525 ± 0.17
5.407GlyAsp: 5.407 ± 0.476
4.357GlyGlu: 4.357 ± 0.335
2.939GlyPhe: 2.939 ± 0.456
6.614GlyGly: 6.614 ± 0.815
1.522GlyHis: 1.522 ± 0.283
4.409GlyIle: 4.409 ± 0.669
4.199GlyLys: 4.199 ± 0.429
6.509GlyLeu: 6.509 ± 1.379
2.939GlyMet: 2.939 ± 0.492
3.202GlyAsn: 3.202 ± 0.459
2.73GlyPro: 2.73 ± 0.399
2.415GlyGln: 2.415 ± 0.343
3.254GlyArg: 3.254 ± 0.337
5.144GlySer: 5.144 ± 0.53
5.512GlyThr: 5.512 ± 0.462
6.719GlyVal: 6.719 ± 0.787
0.892GlyTrp: 0.892 ± 0.244
2.52GlyTyr: 2.52 ± 0.372
0.0GlyXaa: 0.0 ± 0.0
His
1.522HisAla: 1.522 ± 0.34
0.105HisCys: 0.105 ± 0.071
1.05HisAsp: 1.05 ± 0.261
1.417HisGlu: 1.417 ± 0.287
0.367HisPhe: 0.367 ± 0.13
1.365HisGly: 1.365 ± 0.365
0.577HisHis: 0.577 ± 0.192
1.942HisIle: 1.942 ± 0.345
0.997HisLys: 0.997 ± 0.259
1.785HisLeu: 1.785 ± 0.286
0.42HisMet: 0.42 ± 0.159
0.63HisAsn: 0.63 ± 0.211
0.945HisPro: 0.945 ± 0.232
0.525HisGln: 0.525 ± 0.148
1.365HisArg: 1.365 ± 0.269
1.312HisSer: 1.312 ± 0.338
1.05HisThr: 1.05 ± 0.248
1.26HisVal: 1.26 ± 0.309
0.21HisTrp: 0.21 ± 0.101
1.05HisTyr: 1.05 ± 0.237
0.0HisXaa: 0.0 ± 0.0
Ile
5.879IleAla: 5.879 ± 0.55
0.472IleCys: 0.472 ± 0.158
4.042IleAsp: 4.042 ± 0.419
3.832IleGlu: 3.832 ± 0.464
1.785IlePhe: 1.785 ± 0.378
5.249IleGly: 5.249 ± 1.021
1.522IleHis: 1.522 ± 0.35
3.149IleIle: 3.149 ± 0.482
3.254IleLys: 3.254 ± 0.383
5.092IleLeu: 5.092 ± 0.551
1.155IleMet: 1.155 ± 0.258
1.785IleAsn: 1.785 ± 0.305
2.834IlePro: 2.834 ± 0.302
2.205IleGln: 2.205 ± 0.301
3.097IleArg: 3.097 ± 0.419
3.149IleSer: 3.149 ± 0.396
3.569IleThr: 3.569 ± 0.476
4.619IleVal: 4.619 ± 0.412
0.84IleTrp: 0.84 ± 0.195
1.785IleTyr: 1.785 ± 0.279
0.0IleXaa: 0.0 ± 0.0
Lys
5.302LysAla: 5.302 ± 0.563
0.21LysCys: 0.21 ± 0.099
3.937LysAsp: 3.937 ± 0.573
3.832LysGlu: 3.832 ± 0.474
2.1LysPhe: 2.1 ± 0.41
4.462LysGly: 4.462 ± 0.495
1.365LysHis: 1.365 ± 0.286
3.622LysIle: 3.622 ± 0.425
3.779LysLys: 3.779 ± 0.609
5.039LysLeu: 5.039 ± 0.58
1.785LysMet: 1.785 ± 0.315
2.887LysAsn: 2.887 ± 0.35
2.52LysPro: 2.52 ± 0.42
2.467LysGln: 2.467 ± 0.33
2.73LysArg: 2.73 ± 0.412
3.779LysSer: 3.779 ± 0.529
3.674LysThr: 3.674 ± 0.405
4.514LysVal: 4.514 ± 0.569
0.84LysTrp: 0.84 ± 0.195
1.365LysTyr: 1.365 ± 0.29
0.0LysXaa: 0.0 ± 0.0
Leu
7.191LeuAla: 7.191 ± 1.208
0.42LeuCys: 0.42 ± 0.124
5.092LeuAsp: 5.092 ± 0.492
4.567LeuGlu: 4.567 ± 0.474
2.572LeuPhe: 2.572 ± 0.37
5.931LeuGly: 5.931 ± 1.086
1.26LeuHis: 1.26 ± 0.236
4.462LeuIle: 4.462 ± 0.587
5.617LeuLys: 5.617 ± 0.571
5.512LeuLeu: 5.512 ± 0.539
1.785LeuMet: 1.785 ± 0.315
4.409LeuAsn: 4.409 ± 0.565
2.52LeuPro: 2.52 ± 0.366
2.362LeuGln: 2.362 ± 0.412
4.357LeuArg: 4.357 ± 0.451
6.719LeuSer: 6.719 ± 0.645
6.719LeuThr: 6.719 ± 0.623
5.459LeuVal: 5.459 ± 0.666
0.787LeuTrp: 0.787 ± 0.23
2.782LeuTyr: 2.782 ± 0.5
0.0LeuXaa: 0.0 ± 0.0
Met
3.149MetAla: 3.149 ± 0.441
0.105MetCys: 0.105 ± 0.079
1.627MetAsp: 1.627 ± 0.255
1.47MetGlu: 1.47 ± 0.281
0.787MetPhe: 0.787 ± 0.17
2.152MetGly: 2.152 ± 0.345
0.787MetHis: 0.787 ± 0.218
1.575MetIle: 1.575 ± 0.241
1.417MetLys: 1.417 ± 0.267
2.73MetLeu: 2.73 ± 0.346
0.735MetMet: 0.735 ± 0.171
1.26MetAsn: 1.26 ± 0.269
1.26MetPro: 1.26 ± 0.219
0.787MetGln: 0.787 ± 0.183
1.995MetArg: 1.995 ± 0.293
1.995MetSer: 1.995 ± 0.334
2.52MetThr: 2.52 ± 0.296
1.732MetVal: 1.732 ± 0.201
0.262MetTrp: 0.262 ± 0.104
0.577MetTyr: 0.577 ± 0.168
0.0MetXaa: 0.0 ± 0.0
Asn
3.517AsnAla: 3.517 ± 0.408
0.315AsnCys: 0.315 ± 0.139
2.992AsnAsp: 2.992 ± 0.359
3.569AsnGlu: 3.569 ± 0.417
1.522AsnPhe: 1.522 ± 0.37
3.149AsnGly: 3.149 ± 0.441
1.05AsnHis: 1.05 ± 0.255
2.467AsnIle: 2.467 ± 0.342
2.205AsnLys: 2.205 ± 0.419
3.517AsnLeu: 3.517 ± 0.48
0.997AsnMet: 0.997 ± 0.212
1.89AsnAsn: 1.89 ± 0.348
2.572AsnPro: 2.572 ± 0.396
1.47AsnGln: 1.47 ± 0.215
2.73AsnArg: 2.73 ± 0.374
2.992AsnSer: 2.992 ± 0.399
2.625AsnThr: 2.625 ± 0.319
2.362AsnVal: 2.362 ± 0.386
0.367AsnTrp: 0.367 ± 0.151
1.26AsnTyr: 1.26 ± 0.266
0.0AsnXaa: 0.0 ± 0.0
Pro
3.517ProAla: 3.517 ± 0.463
0.367ProCys: 0.367 ± 0.124
2.677ProAsp: 2.677 ± 0.36
3.727ProGlu: 3.727 ± 0.465
1.627ProPhe: 1.627 ± 0.277
3.674ProGly: 3.674 ± 0.453
0.997ProHis: 0.997 ± 0.234
1.837ProIle: 1.837 ± 0.234
2.1ProLys: 2.1 ± 0.274
2.625ProLeu: 2.625 ± 0.282
1.155ProMet: 1.155 ± 0.19
1.47ProAsn: 1.47 ± 0.233
2.625ProPro: 2.625 ± 0.48
1.417ProGln: 1.417 ± 0.303
1.942ProArg: 1.942 ± 0.329
2.834ProSer: 2.834 ± 0.487
2.73ProThr: 2.73 ± 0.411
2.992ProVal: 2.992 ± 0.421
0.42ProTrp: 0.42 ± 0.153
1.942ProTyr: 1.942 ± 0.372
0.0ProXaa: 0.0 ± 0.0
Gln
3.149GlnAla: 3.149 ± 0.389
0.157GlnCys: 0.157 ± 0.098
1.575GlnAsp: 1.575 ± 0.275
1.522GlnGlu: 1.522 ± 0.254
1.155GlnPhe: 1.155 ± 0.184
2.152GlnGly: 2.152 ± 0.503
1.102GlnHis: 1.102 ± 0.247
2.362GlnIle: 2.362 ± 0.374
2.677GlnLys: 2.677 ± 0.357
3.727GlnLeu: 3.727 ± 0.399
1.26GlnMet: 1.26 ± 0.231
1.627GlnAsn: 1.627 ± 0.263
1.365GlnPro: 1.365 ± 0.335
1.522GlnGln: 1.522 ± 0.318
2.152GlnArg: 2.152 ± 0.336
1.732GlnSer: 1.732 ± 0.263
1.995GlnThr: 1.995 ± 0.268
2.362GlnVal: 2.362 ± 0.277
0.472GlnTrp: 0.472 ± 0.202
1.05GlnTyr: 1.05 ± 0.281
0.0GlnXaa: 0.0 ± 0.0
Arg
2.992ArgAla: 2.992 ± 0.375
0.21ArgCys: 0.21 ± 0.109
2.73ArgAsp: 2.73 ± 0.374
3.464ArgGlu: 3.464 ± 0.335
1.575ArgPhe: 1.575 ± 0.304
3.307ArgGly: 3.307 ± 0.445
1.26ArgHis: 1.26 ± 0.276
2.887ArgIle: 2.887 ± 0.387
3.727ArgLys: 3.727 ± 0.531
4.252ArgLeu: 4.252 ± 0.48
1.47ArgMet: 1.47 ± 0.295
2.834ArgAsn: 2.834 ± 0.396
3.097ArgPro: 3.097 ± 0.486
2.047ArgGln: 2.047 ± 0.389
3.149ArgArg: 3.149 ± 0.556
2.415ArgSer: 2.415 ± 0.382
2.834ArgThr: 2.834 ± 0.364
3.517ArgVal: 3.517 ± 0.447
0.892ArgTrp: 0.892 ± 0.295
1.417ArgTyr: 1.417 ± 0.25
0.0ArgXaa: 0.0 ± 0.0
Ser
4.567SerAla: 4.567 ± 0.669
0.315SerCys: 0.315 ± 0.149
3.989SerAsp: 3.989 ± 0.518
4.987SerGlu: 4.987 ± 0.455
1.47SerPhe: 1.47 ± 0.263
5.302SerGly: 5.302 ± 0.502
0.84SerHis: 0.84 ± 0.176
3.622SerIle: 3.622 ± 0.391
4.304SerLys: 4.304 ± 0.503
5.774SerLeu: 5.774 ± 0.6
1.575SerMet: 1.575 ± 0.294
2.677SerAsn: 2.677 ± 0.421
2.205SerPro: 2.205 ± 0.341
2.31SerGln: 2.31 ± 0.298
2.152SerArg: 2.152 ± 0.293
4.252SerSer: 4.252 ± 0.76
4.252SerThr: 4.252 ± 0.588
3.989SerVal: 3.989 ± 0.37
1.155SerTrp: 1.155 ± 0.235
2.31SerTyr: 2.31 ± 0.421
0.0SerXaa: 0.0 ± 0.0
Thr
5.984ThrAla: 5.984 ± 0.622
0.105ThrCys: 0.105 ± 0.075
3.517ThrAsp: 3.517 ± 0.299
4.147ThrGlu: 4.147 ± 0.453
2.257ThrPhe: 2.257 ± 0.319
4.987ThrGly: 4.987 ± 0.448
1.207ThrHis: 1.207 ± 0.285
4.567ThrIle: 4.567 ± 0.532
3.464ThrLys: 3.464 ± 0.388
4.567ThrLeu: 4.567 ± 0.495
1.365ThrMet: 1.365 ± 0.33
2.362ThrAsn: 2.362 ± 0.342
3.517ThrPro: 3.517 ± 0.478
1.732ThrGln: 1.732 ± 0.307
3.044ThrArg: 3.044 ± 0.411
4.094ThrSer: 4.094 ± 0.424
5.249ThrThr: 5.249 ± 0.626
4.514ThrVal: 4.514 ± 0.504
0.577ThrTrp: 0.577 ± 0.153
2.362ThrTyr: 2.362 ± 0.456
0.0ThrXaa: 0.0 ± 0.0
Val
6.824ValAla: 6.824 ± 0.635
0.577ValCys: 0.577 ± 0.204
4.252ValAsp: 4.252 ± 0.434
4.882ValGlu: 4.882 ± 0.431
2.939ValPhe: 2.939 ± 0.478
4.934ValGly: 4.934 ± 0.629
0.787ValHis: 0.787 ± 0.194
4.882ValIle: 4.882 ± 0.482
4.147ValLys: 4.147 ± 0.576
5.092ValLeu: 5.092 ± 0.485
1.732ValMet: 1.732 ± 0.264
2.572ValAsn: 2.572 ± 0.38
3.044ValPro: 3.044 ± 0.361
2.415ValGln: 2.415 ± 0.409
3.779ValArg: 3.779 ± 0.54
4.409ValSer: 4.409 ± 0.422
4.672ValThr: 4.672 ± 0.523
4.777ValVal: 4.777 ± 0.501
0.945ValTrp: 0.945 ± 0.183
2.677ValTyr: 2.677 ± 0.423
0.0ValXaa: 0.0 ± 0.0
Trp
1.102TrpAla: 1.102 ± 0.22
0.105TrpCys: 0.105 ± 0.075
0.525TrpAsp: 0.525 ± 0.168
0.787TrpGlu: 0.787 ± 0.16
0.682TrpPhe: 0.682 ± 0.199
1.05TrpGly: 1.05 ± 0.226
0.262TrpHis: 0.262 ± 0.11
0.472TrpIle: 0.472 ± 0.175
0.577TrpLys: 0.577 ± 0.163
1.155TrpLeu: 1.155 ± 0.24
0.315TrpMet: 0.315 ± 0.125
0.997TrpAsn: 0.997 ± 0.209
0.63TrpPro: 0.63 ± 0.227
0.315TrpGln: 0.315 ± 0.114
0.787TrpArg: 0.787 ± 0.233
0.84TrpSer: 0.84 ± 0.221
0.945TrpThr: 0.945 ± 0.226
0.63TrpVal: 0.63 ± 0.159
0.21TrpTrp: 0.21 ± 0.113
0.472TrpTyr: 0.472 ± 0.141
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.415TyrAla: 2.415 ± 0.354
0.315TyrCys: 0.315 ± 0.11
1.942TyrAsp: 1.942 ± 0.401
1.837TyrGlu: 1.837 ± 0.396
1.522TyrPhe: 1.522 ± 0.354
2.572TyrGly: 2.572 ± 0.376
0.63TyrHis: 0.63 ± 0.148
1.942TyrIle: 1.942 ± 0.359
1.89TyrLys: 1.89 ± 0.371
2.677TyrLeu: 2.677 ± 0.337
0.787TyrMet: 0.787 ± 0.224
1.942TyrAsn: 1.942 ± 0.314
1.417TyrPro: 1.417 ± 0.3
1.05TyrGln: 1.05 ± 0.242
1.785TyrArg: 1.785 ± 0.33
2.467TyrSer: 2.467 ± 0.471
1.68TyrThr: 1.68 ± 0.373
2.362TyrVal: 2.362 ± 0.288
0.315TyrTrp: 0.315 ± 0.105
0.84TyrTyr: 0.84 ± 0.234
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 86 proteins (19052 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski