Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Spirochaetales; Borreliaceae; Borreliella; Borreliella burgdorferi

Average proteome isoelectric point is 7.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1290 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|O51298|O51298_BORBU Exported protein OS=Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680) OX=224326 GN=BB_0319 PE=3 SV=1
MM1 pKa = 7.57KK2 pKa = 10.5GFLAIKK8 pKa = 10.18LSNNEE13 pKa = 4.05YY14 pKa = 10.85YY15 pKa = 9.92PVLNLDD21 pKa = 3.76DD22 pKa = 5.34SSVKK26 pKa = 10.35RR27 pKa = 11.84IILGKK32 pKa = 10.04VAGDD36 pKa = 3.27TSIKK40 pKa = 10.37LNLYY44 pKa = 8.5VSEE47 pKa = 4.84YY48 pKa = 10.96EE49 pKa = 4.78DD50 pKa = 3.89FLDD53 pKa = 4.11PFLVGSFFLDD63 pKa = 3.45NLKK66 pKa = 10.63KK67 pKa = 10.64EE68 pKa = 4.3SLSVNVYY75 pKa = 9.42FKK77 pKa = 11.24VVDD80 pKa = 4.6EE81 pKa = 4.05ILYY84 pKa = 10.73VYY86 pKa = 10.62GEE88 pKa = 4.12SDD90 pKa = 4.62GIQDD94 pKa = 3.58KK95 pKa = 11.51SKK97 pKa = 11.09FDD99 pKa = 3.88LSLVDD104 pKa = 5.2FNQNLKK110 pKa = 10.55GSNRR114 pKa = 11.84GNPLNDD120 pKa = 3.7FKK122 pKa = 11.75SDD124 pKa = 3.17IGSDD128 pKa = 3.49LSVEE132 pKa = 4.18NLGSEE137 pKa = 4.07FDD139 pKa = 4.35LNFEE143 pKa = 4.34EE144 pKa = 6.32KK145 pKa = 10.74DD146 pKa = 3.65DD147 pKa = 4.0FVKK150 pKa = 10.78KK151 pKa = 10.45DD152 pKa = 3.51VSDD155 pKa = 3.77VSMPTINSLDD165 pKa = 3.71LKK167 pKa = 10.13TEE169 pKa = 3.93EE170 pKa = 4.24DD171 pKa = 3.61QKK173 pKa = 11.27IEE175 pKa = 4.3TNVSDD180 pKa = 6.53FEE182 pKa = 5.55DD183 pKa = 4.65FDD185 pKa = 6.05DD186 pKa = 5.67NYY188 pKa = 11.51DD189 pKa = 3.95DD190 pKa = 5.5SLVDD194 pKa = 3.79SKK196 pKa = 11.61KK197 pKa = 11.11LNLEE201 pKa = 4.1NKK203 pKa = 8.42KK204 pKa = 10.32VEE206 pKa = 4.38YY207 pKa = 9.94IDD209 pKa = 4.45EE210 pKa = 4.54KK211 pKa = 11.38SLNLNNFEE219 pKa = 4.04FQKK222 pKa = 10.97LEE224 pKa = 3.94EE225 pKa = 4.42SSLEE229 pKa = 4.05EE230 pKa = 4.19EE231 pKa = 4.68DD232 pKa = 4.73LHH234 pKa = 7.74DD235 pKa = 5.91LSVEE239 pKa = 4.62DD240 pKa = 5.46IIADD244 pKa = 3.4IDD246 pKa = 3.78KK247 pKa = 10.83EE248 pKa = 4.44LIDD251 pKa = 4.04TSDD254 pKa = 3.54EE255 pKa = 4.18DD256 pKa = 3.87VEE258 pKa = 5.07LDD260 pKa = 4.11LRR262 pKa = 11.84EE263 pKa = 4.59DD264 pKa = 4.05FEE266 pKa = 6.44NDD268 pKa = 2.87MVKK271 pKa = 10.93NNFKK275 pKa = 10.82LNSSEE280 pKa = 4.19GMVHH284 pKa = 7.35SPMLYY289 pKa = 10.27LSLISLFLLIFFSLFLVFSKK309 pKa = 10.39ILKK312 pKa = 9.44PRR314 pKa = 11.84DD315 pKa = 3.15FVSYY319 pKa = 10.58YY320 pKa = 10.59FEE322 pKa = 4.21YY323 pKa = 10.82DD324 pKa = 2.69KK325 pKa = 11.64NRR327 pKa = 11.84IKK329 pKa = 10.51RR330 pKa = 11.84YY331 pKa = 10.1EE332 pKa = 3.83KK333 pKa = 10.43DD334 pKa = 3.13QYY336 pKa = 10.7VV337 pKa = 2.99

Molecular weight: 39.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|O51662|SYT_BORBU Threonine--tRNA ligase OS=Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680) OX=224326 GN=thrS PE=3 SV=1
MM1 pKa = 7.67AVPKK5 pKa = 9.82FKK7 pKa = 10.16PSKK10 pKa = 9.35SRR12 pKa = 11.84SRR14 pKa = 11.84TRR16 pKa = 11.84RR17 pKa = 11.84SINMRR22 pKa = 11.84KK23 pKa = 9.83KK24 pKa = 9.1IPQFQEE30 pKa = 3.95CSNCGNLGVRR40 pKa = 11.84HH41 pKa = 6.67RR42 pKa = 11.84ICLKK46 pKa = 9.99CGYY49 pKa = 9.26YY50 pKa = 10.42RR51 pKa = 11.84NNQYY55 pKa = 11.29LEE57 pKa = 4.01IGLL60 pKa = 4.11

Molecular weight: 7.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1290

0

1290

381647

37

2166

295.9

33.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.52 ± 0.065

0.744 ± 0.022

5.259 ± 0.062

7.014 ± 0.075

5.937 ± 0.073

4.893 ± 0.082

1.255 ± 0.022

10.294 ± 0.084

10.745 ± 0.087

10.217 ± 0.076

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.876 ± 0.026

7.528 ± 0.078

2.431 ± 0.042

2.538 ± 0.037

3.207 ± 0.044

7.41 ± 0.061

4.199 ± 0.054

5.102 ± 0.065

0.473 ± 0.016

4.352 ± 0.058
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski