Methanothrix soehngenii (strain ATCC 5969 / DSM 3671 / JCM 10134 / NBRC 103675 / OCM 69 / GP-6) (Methanosaeta concilii)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia; Methanosarcinales; Methanotrichaceae; Methanothrix; Methanothrix soehngenii

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2791 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F4BT93|F4BT93_METSG Fibronectin type-III domain-containing protein OS=Methanothrix soehngenii (strain ATCC 5969 / DSM 3671 / JCM 10134 / NBRC 103675 / OCM 69 / GP-6) OX=990316 GN=MCON_0003 PE=4 SV=1
MM1 pKa = 7.94DD2 pKa = 3.79IVSRR6 pKa = 11.84KK7 pKa = 9.97YY8 pKa = 10.89GIASLIALILIAFGFNAAGAEE29 pKa = 3.99YY30 pKa = 10.71SLGSEE35 pKa = 4.47SDD37 pKa = 4.0DD38 pKa = 2.93WWKK41 pKa = 10.7EE42 pKa = 3.77YY43 pKa = 10.22PDD45 pKa = 3.55QSTGAGGDD53 pKa = 3.39VSHH56 pKa = 7.68PDD58 pKa = 3.06WVLDD62 pKa = 3.78ALEE65 pKa = 4.61SKK67 pKa = 10.46PVLIYY72 pKa = 8.9VHH74 pKa = 6.85KK75 pKa = 10.29EE76 pKa = 3.07CDD78 pKa = 3.39YY79 pKa = 11.26CVPQTEE85 pKa = 4.36AVQKK89 pKa = 10.72IIDD92 pKa = 4.07EE93 pKa = 4.39FSGEE97 pKa = 3.78ITIFEE102 pKa = 4.65LGADD106 pKa = 3.8GSDD109 pKa = 3.46VRR111 pKa = 11.84SGEE114 pKa = 3.91ALQAYY119 pKa = 8.53DD120 pKa = 4.1PNGGLDD126 pKa = 3.4MFVPLTVILTLAPDD140 pKa = 3.62SEE142 pKa = 4.78GEE144 pKa = 4.2IVAVWHH150 pKa = 6.55SSDD153 pKa = 4.09EE154 pKa = 4.15ITGEE158 pKa = 3.69EE159 pKa = 4.33WIRR162 pKa = 11.84NYY164 pKa = 10.47VEE166 pKa = 4.94DD167 pKa = 5.78AISLYY172 pKa = 10.87DD173 pKa = 3.77EE174 pKa = 5.97NYY176 pKa = 11.01ADD178 pKa = 3.85WDD180 pKa = 3.75NN181 pKa = 3.47

Molecular weight: 19.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F4BTI6|F4BTI6_METSG Type 2 DNA topoisomerase 6 subunit A OS=Methanothrix soehngenii (strain ATCC 5969 / DSM 3671 / JCM 10134 / NBRC 103675 / OCM 69 / GP-6) OX=990316 GN=top6a PE=3 SV=1
MM1 pKa = 7.4NWTPLGGDD9 pKa = 3.38YY10 pKa = 10.54RR11 pKa = 11.84RR12 pKa = 11.84QKK14 pKa = 10.63LYY16 pKa = 11.1NRR18 pKa = 11.84GILVKK23 pKa = 10.67NPRR26 pKa = 11.84ICLAISHH33 pKa = 7.35LDD35 pKa = 3.57CMDD38 pKa = 3.79NGLLRR43 pKa = 11.84NHH45 pKa = 7.02LAVAEE50 pKa = 4.36EE51 pKa = 3.83WRR53 pKa = 11.84MRR55 pKa = 11.84MVPSAHH61 pKa = 6.9CSDD64 pKa = 4.11TSPEE68 pKa = 3.91RR69 pKa = 11.84TRR71 pKa = 11.84KK72 pKa = 9.35GRR74 pKa = 11.84RR75 pKa = 11.84SRR77 pKa = 3.52

Molecular weight: 9.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2791

0

2791

821985

37

3064

294.5

32.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.471 ± 0.055

1.301 ± 0.026

5.829 ± 0.039

7.178 ± 0.045

3.634 ± 0.032

7.683 ± 0.047

1.723 ± 0.022

7.222 ± 0.04

5.306 ± 0.05

9.877 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.859 ± 0.023

3.553 ± 0.036

4.255 ± 0.035

3.024 ± 0.027

5.903 ± 0.041

6.82 ± 0.047

4.514 ± 0.038

6.564 ± 0.042

1.156 ± 0.019

3.127 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski