Erythrobacter litoralis (strain HTCC2594)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Erythrobacteraceae; Erythrobacter/Porphyrobacter group; Erythrobacter; Erythrobacter litoralis

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2999 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q2N5Y4|Q2N5Y4_ERYLH Serine palmitoyltransferase OS=Erythrobacter litoralis (strain HTCC2594) OX=314225 GN=ELI_14075 PE=3 SV=1
MM1 pKa = 7.18ATADD5 pKa = 4.47LEE7 pKa = 4.39YY8 pKa = 10.57LYY10 pKa = 11.04AALLQDD16 pKa = 3.66MVSADD21 pKa = 4.1EE22 pKa = 4.06QSLALTEE29 pKa = 4.16RR30 pKa = 11.84LRR32 pKa = 11.84DD33 pKa = 3.39AATSEE38 pKa = 4.21DD39 pKa = 4.14LRR41 pKa = 11.84DD42 pKa = 3.68ALEE45 pKa = 5.24DD46 pKa = 4.14GVCGIGDD53 pKa = 3.76GLEE56 pKa = 4.08IARR59 pKa = 11.84KK60 pKa = 8.87LAEE63 pKa = 4.31AHH65 pKa = 6.6PADD68 pKa = 4.73DD69 pKa = 6.45GEE71 pKa = 4.3FQSDD75 pKa = 3.3GMAGLVTDD83 pKa = 3.45TQEE86 pKa = 4.41RR87 pKa = 11.84VFEE90 pKa = 4.17TDD92 pKa = 3.38FASGSARR99 pKa = 11.84DD100 pKa = 3.72AAILAQYY107 pKa = 8.35MHH109 pKa = 6.81LTYY112 pKa = 10.68YY113 pKa = 11.0GLAGYY118 pKa = 7.61RR119 pKa = 11.84TLAAIASQLGHH130 pKa = 7.07ADD132 pKa = 3.53EE133 pKa = 6.58AEE135 pKa = 4.17DD136 pKa = 5.14LQTCYY141 pKa = 10.93EE142 pKa = 4.07NAQDD146 pKa = 4.77GQDD149 pKa = 4.2DD150 pKa = 4.67MIGLLDD156 pKa = 4.04GEE158 pKa = 4.61VLEE161 pKa = 4.93DD162 pKa = 3.54TAA164 pKa = 5.9

Molecular weight:
17.59 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q2NC79|Q2NC79_ERYLH Biotin carboxylase OS=Erythrobacter litoralis (strain HTCC2594) OX=314225 GN=ELI_03100 PE=4 SV=1
MM1 pKa = 7.1KK2 pKa = 10.16HH3 pKa = 6.16RR4 pKa = 11.84RR5 pKa = 11.84QTLLMILMRR14 pKa = 11.84FSIRR18 pKa = 11.84IWFVLSRR25 pKa = 11.84WLISLNRR32 pKa = 11.84RR33 pKa = 11.84HH34 pKa = 6.16

Molecular weight:
4.41 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2999

0

2999

928556

23

2409

309.6

33.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.305 ± 0.071

0.812 ± 0.014

6.349 ± 0.038

6.605 ± 0.047

3.699 ± 0.031

8.802 ± 0.044

1.915 ± 0.023

5.088 ± 0.029

3.326 ± 0.043

9.627 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.488 ± 0.028

2.628 ± 0.031

5.083 ± 0.035

3.222 ± 0.024

6.888 ± 0.04

5.341 ± 0.037

5.232 ± 0.044

6.943 ± 0.04

1.432 ± 0.02

2.216 ± 0.025

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski