Shrew coronavirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; unclassified Orthocoronavirinae

Average proteome isoelectric point is 7.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4MZ81|A0A2H4MZ81_9NIDO 2'-O-methyltransferase OS=Shrew coronavirus OX=2050019 PE=3 SV=1
MM1 pKa = 7.76LILVFLSLVTVSLQNVVVPFDD22 pKa = 3.68NYY24 pKa = 10.96CNLDD28 pKa = 3.34ARR30 pKa = 11.84YY31 pKa = 10.07HH32 pKa = 6.2NITVQSEE39 pKa = 4.28KK40 pKa = 10.58PFNFNATFYY49 pKa = 7.63TTNQTCFDD57 pKa = 4.17VNCTSSNTSNTFNSSDD73 pKa = 3.55TITYY77 pKa = 9.29SNCSSVTFNGCVVKK91 pKa = 9.04QFCVTQPCHH100 pKa = 6.92CYY102 pKa = 10.34YY103 pKa = 9.58QTQQVSGFVFDD114 pKa = 4.53GLSHH118 pKa = 6.28TFVPEE123 pKa = 3.53ISNYY127 pKa = 9.62HH128 pKa = 5.48VADD131 pKa = 3.85VVNKK135 pKa = 9.29TLTRR139 pKa = 11.84TTNLCSSVNVTIDD152 pKa = 3.29YY153 pKa = 10.49KK154 pKa = 10.83QFLMLSSVEE163 pKa = 4.06INSTFVVTQFVPSYY177 pKa = 11.11VYY179 pKa = 10.81NQDD182 pKa = 2.79NFTIKK187 pKa = 10.63GDD189 pKa = 3.96NFWVVANTTTVFVLQPKK206 pKa = 8.62TPSNEE211 pKa = 4.08TLSLYY216 pKa = 10.32IKK218 pKa = 9.08ITNSSGSLFVNVTICNYY235 pKa = 9.65FRR237 pKa = 11.84VLNNTDD243 pKa = 3.21NTNTSSLVYY252 pKa = 8.51TNCVFEE258 pKa = 4.47KK259 pKa = 10.71VAGGLQVVTLGVIWRR274 pKa = 11.84KK275 pKa = 9.55NVVTLHH281 pKa = 6.04WLAQEE286 pKa = 4.29VVTIEE291 pKa = 5.42LPFDD295 pKa = 3.92PQWYY299 pKa = 10.12GGDD302 pKa = 3.46PALNGLYY309 pKa = 10.72CNGWYY314 pKa = 10.42ANSLEE319 pKa = 4.32SPHH322 pKa = 6.77TYY324 pKa = 10.59LLKK327 pKa = 10.23TNASGHH333 pKa = 5.7IVNSLWCNPDD343 pKa = 4.07DD344 pKa = 4.33EE345 pKa = 5.72LSSLQCAYY353 pKa = 9.07KK354 pKa = 10.25TFNLNRR360 pKa = 11.84GVYY363 pKa = 10.01SRR365 pKa = 11.84TVHH368 pKa = 5.56QKK370 pKa = 9.39VVYY373 pKa = 9.23LQRR376 pKa = 11.84FSSTPKK382 pKa = 10.37CVTNKK387 pKa = 9.55VNADD391 pKa = 3.49LVVRR395 pKa = 11.84GGFYY399 pKa = 10.07ATLTNCDD406 pKa = 3.52YY407 pKa = 11.37DD408 pKa = 3.82LHH410 pKa = 7.26GLLNISLLPQRR421 pKa = 11.84WGGVNYY427 pKa = 7.78TIPPVVVPWVLKK439 pKa = 11.06NPDD442 pKa = 3.1GTSRR446 pKa = 11.84LWQQCVGVDD455 pKa = 3.39VASANTQCFKK465 pKa = 10.82SISVSFVTNAYY476 pKa = 10.37LLEE479 pKa = 4.55LLPGQCVSYY488 pKa = 10.72YY489 pKa = 10.98GYY491 pKa = 8.63FTTGAAGLVAKK502 pKa = 10.24NAIVGTFDD510 pKa = 4.4LSSGDD515 pKa = 3.84TQTCMYY521 pKa = 9.23QQQNTTFEE529 pKa = 4.27IPQNVCVSFTFDD541 pKa = 2.91SQTIKK546 pKa = 11.22GILTQFNTTYY556 pKa = 10.2PSNYY560 pKa = 9.31TNILKK565 pKa = 10.7HH566 pKa = 6.0NGVVKK571 pKa = 9.11YY572 pKa = 10.53VRR574 pKa = 11.84IDD576 pKa = 3.32GVSYY580 pKa = 10.29VVKK583 pKa = 9.69PCLFEE588 pKa = 4.49SVVKK592 pKa = 10.45IVNNATYY599 pKa = 10.09NHH601 pKa = 6.96TIISTSLCGEE611 pKa = 4.05NGTNTTIGCIQTGFVNEE628 pKa = 4.23TLNTCDD634 pKa = 3.49YY635 pKa = 11.18SLGNGFCANVNKK647 pKa = 10.8SMDD650 pKa = 3.5VYY652 pKa = 11.34KK653 pKa = 10.42FIPSKK658 pKa = 9.21QTNDD662 pKa = 3.26YY663 pKa = 9.71VVPISGMYY671 pKa = 10.53NITLPTSYY679 pKa = 11.14EE680 pKa = 4.12PFTSTAYY687 pKa = 10.14YY688 pKa = 7.27QTRR691 pKa = 11.84LEE693 pKa = 4.5KK694 pKa = 10.89LQVDD698 pKa = 4.45CQHH701 pKa = 6.4FVCDD705 pKa = 4.58SNIHH709 pKa = 6.43CLTLLQKK716 pKa = 11.12YY717 pKa = 10.68GDD719 pKa = 3.92ICTKK723 pKa = 9.28ITGDD727 pKa = 3.72VNTVMASLTSLEE739 pKa = 4.29LDD741 pKa = 3.42ALQTVALEE749 pKa = 4.21GLNVDD754 pKa = 3.48YY755 pKa = 11.4GSFNFSGLMPQRR767 pKa = 11.84GTRR770 pKa = 11.84SFIEE774 pKa = 4.61DD775 pKa = 3.87LLFDD779 pKa = 5.23KK780 pKa = 10.73IVTTGPQFYY789 pKa = 10.3KK790 pKa = 10.46DD791 pKa = 4.14YY792 pKa = 10.92YY793 pKa = 10.63DD794 pKa = 3.49CHH796 pKa = 7.18KK797 pKa = 11.1DD798 pKa = 3.19KK799 pKa = 10.96TINDD803 pKa = 4.53LLCAQQYY810 pKa = 11.09NGMLVVPPIMDD821 pKa = 4.59DD822 pKa = 3.21ATIAMYY828 pKa = 8.41GTIAGASVSAGMFGGQAGMITWNTAVSARR857 pKa = 11.84LNALGITQEE866 pKa = 4.17VMQSDD871 pKa = 3.53IQKK874 pKa = 9.95IANAVNGIVDD884 pKa = 3.88HH885 pKa = 6.39VSNLAKK891 pKa = 9.74TVAGGFEE898 pKa = 4.4VIQAVVNQNGAQMMALVDD916 pKa = 3.81GLQEE920 pKa = 3.97NFGAISNNFEE930 pKa = 4.45TINNRR935 pKa = 11.84LNKK938 pKa = 10.35LEE940 pKa = 5.38ADD942 pKa = 3.51QQMDD946 pKa = 3.53RR947 pKa = 11.84LINGRR952 pKa = 11.84LNVLQSFMTNYY963 pKa = 9.61KK964 pKa = 9.75IKK966 pKa = 10.37VSAMQATQHH975 pKa = 5.35TVNSIIKK982 pKa = 8.35EE983 pKa = 4.06CVYY986 pKa = 10.88AQSTRR991 pKa = 11.84NGFCGDD997 pKa = 3.67GKK999 pKa = 10.21HH1000 pKa = 6.37IMSFMQNAPDD1010 pKa = 4.7GIFFIHH1016 pKa = 6.23FTMKK1020 pKa = 9.43PVQYY1024 pKa = 10.42INVTTTPGLCINNTGIAPKK1043 pKa = 10.42DD1044 pKa = 3.69GIFVFVNGTWMVSPRR1059 pKa = 11.84NIYY1062 pKa = 10.18NPRR1065 pKa = 11.84NVSNSDD1071 pKa = 2.62IVHH1074 pKa = 6.34YY1075 pKa = 10.22ISCEE1079 pKa = 3.73ANYY1082 pKa = 9.76TLVNNTIGSVVLPQLPNIGDD1102 pKa = 3.93DD1103 pKa = 4.1FNNLYY1108 pKa = 10.89EE1109 pKa = 4.31NTSKK1113 pKa = 11.11ALEE1116 pKa = 4.46EE1117 pKa = 3.92IKK1119 pKa = 10.76NIYY1122 pKa = 10.52FNFTNLNLTTEE1133 pKa = 4.37IEE1135 pKa = 4.24RR1136 pKa = 11.84LNSIAEE1142 pKa = 4.19EE1143 pKa = 4.2VKK1145 pKa = 10.64KK1146 pKa = 11.19LNITLSEE1153 pKa = 4.03LGRR1156 pKa = 11.84YY1157 pKa = 7.15EE1158 pKa = 6.13RR1159 pKa = 11.84YY1160 pKa = 10.21VKK1162 pKa = 9.79WPWYY1166 pKa = 8.27VWLAIAVVMIVLVFILGCLLFMTGCCGCCGCCGGCCGKK1204 pKa = 10.47ACSKK1208 pKa = 10.62PPDD1211 pKa = 3.64YY1212 pKa = 11.43EE1213 pKa = 4.09EE1214 pKa = 5.02LEE1216 pKa = 4.36KK1217 pKa = 11.27LHH1219 pKa = 6.35VAA1221 pKa = 4.38

Molecular weight: 135.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4MXZ6|A0A2H4MXZ6_9NIDO Spike glycoprotein OS=Shrew coronavirus OX=2050019 PE=4 SV=1
MM1 pKa = 7.42SGKK4 pKa = 9.49SWADD8 pKa = 3.14RR9 pKa = 11.84VEE11 pKa = 5.07AEE13 pKa = 4.1EE14 pKa = 4.52QKK16 pKa = 11.05NKK18 pKa = 8.66PQQRR22 pKa = 11.84RR23 pKa = 11.84SRR25 pKa = 11.84SKK27 pKa = 10.26SKK29 pKa = 10.49DD30 pKa = 3.17RR31 pKa = 11.84KK32 pKa = 8.51TMPLSWFNPLEE43 pKa = 4.07FEE45 pKa = 4.36VGKK48 pKa = 10.5DD49 pKa = 3.38LGDD52 pKa = 4.94LINTNSVPIGKK63 pKa = 8.21GTKK66 pKa = 8.95QEE68 pKa = 3.79QHH70 pKa = 6.75GYY72 pKa = 9.68WNIQTRR78 pKa = 11.84FRR80 pKa = 11.84VNKK83 pKa = 9.47GKK85 pKa = 10.45RR86 pKa = 11.84VDD88 pKa = 3.76LQPRR92 pKa = 11.84AFFYY96 pKa = 9.94YY97 pKa = 10.17TGTGPHH103 pKa = 7.06KK104 pKa = 10.26ILAFGEE110 pKa = 4.22QQEE113 pKa = 4.58GVVWVATKK121 pKa = 10.32EE122 pKa = 3.91ADD124 pKa = 3.45HH125 pKa = 6.46NPVRR129 pKa = 11.84FGDD132 pKa = 3.91RR133 pKa = 11.84PEE135 pKa = 4.08STPAMEE141 pKa = 4.18VKK143 pKa = 10.15IRR145 pKa = 11.84QGKK148 pKa = 8.33QPLGVKK154 pKa = 9.97LLNDD158 pKa = 3.45SKK160 pKa = 11.49EE161 pKa = 4.07EE162 pKa = 3.86VVRR165 pKa = 11.84QSRR168 pKa = 11.84ARR170 pKa = 11.84SRR172 pKa = 11.84SRR174 pKa = 11.84SQSRR178 pKa = 11.84SRR180 pKa = 11.84DD181 pKa = 3.37TSQKK185 pKa = 10.22RR186 pKa = 11.84VTFSDD191 pKa = 3.54QQPKK195 pKa = 9.8GDD197 pKa = 3.55QYY199 pKa = 10.18VTKK202 pKa = 10.74NDD204 pKa = 3.61LSNLLSKK211 pKa = 11.04LLDD214 pKa = 3.61EE215 pKa = 5.35KK216 pKa = 11.33LNSNPKK222 pKa = 8.87PQRR225 pKa = 11.84QPRR228 pKa = 11.84SRR230 pKa = 11.84SNSSTRR236 pKa = 11.84NSQKK240 pKa = 8.91EE241 pKa = 4.13TQAVQKK247 pKa = 10.92DD248 pKa = 3.91QMSKK252 pKa = 9.63HH253 pKa = 3.78WWKK256 pKa = 10.76RR257 pKa = 11.84IPTKK261 pKa = 10.83DD262 pKa = 3.47EE263 pKa = 4.76GIEE266 pKa = 3.73QCFGQRR272 pKa = 11.84SDD274 pKa = 3.33TVNFGTKK281 pKa = 10.5YY282 pKa = 9.89MVDD285 pKa = 3.04QGTGGNFPQLATLLPTPAAMLYY307 pKa = 9.89GSHH310 pKa = 5.76VQITPGMSPDD320 pKa = 3.52KK321 pKa = 10.79EE322 pKa = 4.28FIVYY326 pKa = 8.65TCGIEE331 pKa = 3.95VDD333 pKa = 4.2KK334 pKa = 11.37NDD336 pKa = 5.12PIYY339 pKa = 11.05KK340 pKa = 9.61EE341 pKa = 3.92FKK343 pKa = 10.17KK344 pKa = 10.85GVNAFKK350 pKa = 10.86DD351 pKa = 3.54DD352 pKa = 4.02TTWLSGNTIAKK363 pKa = 9.8AKK365 pKa = 10.07NLNTTSTPSQTVQSVSEE382 pKa = 4.39SNASVVVKK390 pKa = 10.29FDD392 pKa = 3.77EE393 pKa = 4.51NGEE396 pKa = 4.34EE397 pKa = 4.28VSSAA401 pKa = 3.28

Molecular weight: 45.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

8310

78

6381

1662.0

186.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.199 ± 0.358

3.634 ± 0.694

5.884 ± 0.656

3.911 ± 0.594

5.199 ± 0.441

5.716 ± 0.323

1.757 ± 0.131

4.862 ± 1.332

6.751 ± 0.966

8.448 ± 0.67

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.118 ± 0.14

6.258 ± 1.003

3.682 ± 0.299

3.502 ± 0.868

2.78 ± 0.703

6.955 ± 0.55

7.064 ± 0.668

10.301 ± 0.598

1.372 ± 0.238

4.609 ± 0.532
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski