Pseudonocardia dioxanivorans (strain ATCC 55486 / DSM 44775 / JCM 13855 / CB1190)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Pseudonocardia; Pseudonocardia dioxanivorans

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6620 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F4CPW4|F4CPW4_PSEUX GCN5-related N-acetyltransferase OS=Pseudonocardia dioxanivorans (strain ATCC 55486 / DSM 44775 / JCM 13855 / CB1190) OX=675635 GN=Psed_3981 PE=4 SV=1
MM1 pKa = 7.27ATHH4 pKa = 6.86EE5 pKa = 4.6GQWKK9 pKa = 10.26AGTPCWVDD17 pKa = 3.65LNVPDD22 pKa = 4.82VPTATAFYY30 pKa = 10.26SAVLGWSFVDD40 pKa = 3.83TGAEE44 pKa = 3.78FGNYY48 pKa = 9.33QIAQVDD54 pKa = 3.67GRR56 pKa = 11.84AAAGIGPIMQEE67 pKa = 4.12GQPSFWTLYY76 pKa = 10.09LASDD80 pKa = 4.63DD81 pKa = 4.47ADD83 pKa = 3.46ATAKK87 pKa = 10.62LVTDD91 pKa = 3.91NGGSLIAGPMDD102 pKa = 3.24IPGSGRR108 pKa = 11.84MVIATDD114 pKa = 3.11TAGAVFGVWQTLGMNGAEE132 pKa = 4.24VVNEE136 pKa = 3.86PGGLVWEE143 pKa = 4.99DD144 pKa = 3.44ARR146 pKa = 11.84LTDD149 pKa = 4.31PEE151 pKa = 4.24TARR154 pKa = 11.84AFYY157 pKa = 10.44AAVFGYY163 pKa = 9.17TYY165 pKa = 10.68DD166 pKa = 3.81AVDD169 pKa = 4.66GAPEE173 pKa = 5.01DD174 pKa = 4.0YY175 pKa = 7.89TTFALDD181 pKa = 3.66GEE183 pKa = 4.93IKK185 pKa = 10.68GGMGGLMGAPDD196 pKa = 4.45GTPSHH201 pKa = 7.08WIAYY205 pKa = 9.16FGAADD210 pKa = 3.35VDD212 pKa = 3.89AAVAQVQANGGSVMMAPQNTPFGRR236 pKa = 11.84MGIVADD242 pKa = 3.77PFGAPFCLHH251 pKa = 6.61GEE253 pKa = 4.36VTGG256 pKa = 3.99

Molecular weight: 26.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F4CZY9|F4CZY9_PSEUX O-succinylbenzoate--CoA ligase OS=Pseudonocardia dioxanivorans (strain ATCC 55486 / DSM 44775 / JCM 13855 / CB1190) OX=675635 GN=Psed_2619 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.79HH17 pKa = 5.67RR18 pKa = 11.84KK19 pKa = 8.46LLRR22 pKa = 11.84KK23 pKa = 7.95TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.07LGKK33 pKa = 9.87

Molecular weight: 4.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6620

0

6620

2118731

30

7912

320.1

33.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.487 ± 0.046

0.774 ± 0.009

6.447 ± 0.027

5.231 ± 0.03

2.6 ± 0.017

9.639 ± 0.031

2.209 ± 0.015

3.128 ± 0.021

1.417 ± 0.015

10.153 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.685 ± 0.012

1.513 ± 0.014

6.321 ± 0.028

2.408 ± 0.018

8.439 ± 0.033

4.718 ± 0.022

6.025 ± 0.022

9.601 ± 0.032

1.439 ± 0.013

1.767 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski