Amino acid dipepetide frequency for Brachypodium distachyon (Purple false brome) (Trachynia distachya)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	12.622AlaAla: 12.622 ± 0.061	1.592AlaCys: 1.592 ± 0.009	4.189AlaAsp: 4.189 ± 0.018	4.923AlaGlu: 4.923 ± 0.018	2.873AlaPhe: 2.873 ± 0.014	6.392AlaGly: 6.392 ± 0.029	1.785AlaHis: 1.785 ± 0.011	3.566AlaIle: 3.566 ± 0.016	3.741AlaLys: 3.741 ± 0.016	7.79AlaLeu: 7.79 ± 0.029	2.228AlaMet: 2.228 ± 0.011	2.543AlaAsn: 2.543 ± 0.012	4.809AlaPro: 4.809 ± 0.025	2.47AlaGln: 2.47 ± 0.013	5.275AlaArg: 5.275 ± 0.021	7.469AlaSer: 7.469 ± 0.023	4.483AlaThr: 4.483 ± 0.018	6.327AlaVal: 6.327 ± 0.024	1.0AlaTrp: 1.0 ± 0.007	1.893AlaTyr: 1.893 ± 0.011	0.0AlaXaa: 0.0 ± 0.0
Cys	1.385CysAla: 1.385 ± 0.011	0.667CysCys: 0.667 ± 0.008	0.908CysAsp: 0.908 ± 0.008	0.858CysGlu: 0.858 ± 0.007	0.874CysPhe: 0.874 ± 0.008	1.52CysGly: 1.52 ± 0.011	0.549CysHis: 0.549 ± 0.006	0.953CysIle: 0.953 ± 0.008	0.999CysLys: 0.999 ± 0.008	1.908CysLeu: 1.908 ± 0.01	0.475CysMet: 0.475 ± 0.005	0.745CysAsn: 0.745 ± 0.007	1.08CysPro: 1.08 ± 0.009	0.621CysGln: 0.621 ± 0.006	1.379CysArg: 1.379 ± 0.01	1.988CysSer: 1.988 ± 0.013	0.982CysThr: 0.982 ± 0.007	1.156CysVal: 1.156 ± 0.008	0.291CysTrp: 0.291 ± 0.005	0.543CysTyr: 0.543 ± 0.005	0.0CysXaa: 0.0 ± 0.0
Asp	4.655AspAla: 4.655 ± 0.019	0.977AspCys: 0.977 ± 0.009	4.027AspAsp: 4.027 ± 0.025	3.893AspGlu: 3.893 ± 0.018	2.044AspPhe: 2.044 ± 0.012	4.478AspGly: 4.478 ± 0.018	1.283AspHis: 1.283 ± 0.009	2.724AspIle: 2.724 ± 0.013	2.473AspLys: 2.473 ± 0.013	4.933AspLeu: 4.933 ± 0.017	1.415AspMet: 1.415 ± 0.008	1.847AspAsn: 1.847 ± 0.011	2.758AspPro: 2.758 ± 0.015	1.695AspGln: 1.695 ± 0.009	2.732AspArg: 2.732 ± 0.015	3.809AspSer: 3.809 ± 0.017	2.332AspThr: 2.332 ± 0.012	3.728AspVal: 3.728 ± 0.015	0.689AspTrp: 0.689 ± 0.006	1.394AspTyr: 1.394 ± 0.01	0.0AspXaa: 0.0 ± 0.0
Glu	5.264GluAla: 5.264 ± 0.02	0.892GluCys: 0.892 ± 0.008	3.83GluAsp: 3.83 ± 0.018	5.794GluGlu: 5.794 ± 0.032	2.031GluPhe: 2.031 ± 0.011	3.672GluGly: 3.672 ± 0.013	1.434GluHis: 1.434 ± 0.01	3.006GluIle: 3.006 ± 0.016	3.817GluLys: 3.817 ± 0.021	5.863GluLeu: 5.863 ± 0.023	1.648GluMet: 1.648 ± 0.01	2.333GluAsn: 2.333 ± 0.014	2.383GluPro: 2.383 ± 0.014	2.29GluGln: 2.29 ± 0.014	3.483GluArg: 3.483 ± 0.015	3.891GluSer: 3.891 ± 0.017	2.673GluThr: 2.673 ± 0.013	3.967GluVal: 3.967 ± 0.016	0.714GluTrp: 0.714 ± 0.007	1.478GluTyr: 1.478 ± 0.009	0.0GluXaa: 0.0 ± 0.0
Phe	2.733PheAla: 2.733 ± 0.012	0.842PheCys: 0.842 ± 0.007	2.136PheAsp: 2.136 ± 0.011	1.868PheGlu: 1.868 ± 0.011	1.761PhePhe: 1.761 ± 0.011	2.831PheGly: 2.831 ± 0.015	1.033PheHis: 1.033 ± 0.008	1.607PheIle: 1.607 ± 0.01	1.537PheLys: 1.537 ± 0.01	3.974PheLeu: 3.974 ± 0.018	0.865PheMet: 0.865 ± 0.007	1.292PheAsn: 1.292 ± 0.009	1.923PhePro: 1.923 ± 0.011	1.283PheGln: 1.283 ± 0.009	2.112PheArg: 2.112 ± 0.011	3.462PheSer: 3.462 ± 0.016	1.724PheThr: 1.724 ± 0.012	2.623PheVal: 2.623 ± 0.012	0.536PheTrp: 0.536 ± 0.006	1.048PheTyr: 1.048 ± 0.008	0.0PheXaa: 0.0 ± 0.0
Gly	5.875GlyAla: 5.875 ± 0.024	1.489GlyCys: 1.489 ± 0.01	3.875GlyAsp: 3.875 ± 0.014	3.899GlyGlu: 3.899 ± 0.018	2.843GlyPhe: 2.843 ± 0.012	7.317GlyGly: 7.317 ± 0.046	1.789GlyHis: 1.789 ± 0.012	3.094GlyIle: 3.094 ± 0.016	3.645GlyLys: 3.645 ± 0.016	5.968GlyLeu: 5.968 ± 0.021	1.652GlyMet: 1.652 ± 0.01	2.754GlyAsn: 2.754 ± 0.015	2.846GlyPro: 2.846 ± 0.014	2.225GlyGln: 2.225 ± 0.011	4.769GlyArg: 4.769 ± 0.024	6.239GlySer: 6.239 ± 0.025	3.538GlyThr: 3.538 ± 0.015	4.504GlyVal: 4.504 ± 0.017	0.979GlyTrp: 0.979 ± 0.008	1.975GlyTyr: 1.975 ± 0.012	0.0GlyXaa: 0.0 ± 0.0
His	1.94HisAla: 1.94 ± 0.011	0.57HisCys: 0.57 ± 0.006	1.29HisAsp: 1.29 ± 0.008	1.313HisGlu: 1.313 ± 0.009	0.93HisPhe: 0.93 ± 0.007	2.177HisGly: 2.177 ± 0.012	1.024HisHis: 1.024 ± 0.01	1.119HisIle: 1.119 ± 0.009	1.017HisLys: 1.017 ± 0.009	2.584HisLeu: 2.584 ± 0.016	0.59HisMet: 0.59 ± 0.006	0.842HisAsn: 0.842 ± 0.007	1.541HisPro: 1.541 ± 0.009	0.982HisGln: 0.982 ± 0.008	1.756HisArg: 1.756 ± 0.008	1.808HisSer: 1.808 ± 0.01	1.033HisThr: 1.033 ± 0.009	1.667HisVal: 1.667 ± 0.01	0.313HisTrp: 0.313 ± 0.004	0.655HisTyr: 0.655 ± 0.007	0.0HisXaa: 0.0 ± 0.0
Ile	3.457IleAla: 3.457 ± 0.015	1.004IleCys: 1.004 ± 0.008	2.501IleAsp: 2.501 ± 0.012	2.493IleGlu: 2.493 ± 0.012	1.793IlePhe: 1.793 ± 0.012	2.926IleGly: 2.926 ± 0.014	1.125IleHis: 1.125 ± 0.007	2.247IleIle: 2.247 ± 0.013	2.229IleLys: 2.229 ± 0.013	4.432IleLeu: 4.432 ± 0.021	1.019IleMet: 1.019 ± 0.007	1.671IleAsn: 1.671 ± 0.01	2.469IlePro: 2.469 ± 0.016	1.65IleGln: 1.65 ± 0.011	2.441IleArg: 2.441 ± 0.013	3.96IleSer: 3.96 ± 0.018	2.266IleThr: 2.266 ± 0.011	2.996IleVal: 2.996 ± 0.013	0.625IleTrp: 0.625 ± 0.006	1.228IleTyr: 1.228 ± 0.009	0.0IleXaa: 0.0 ± 0.0
Lys	3.789LysAla: 3.789 ± 0.018	0.846LysCys: 0.846 ± 0.007	2.83LysAsp: 2.83 ± 0.013	3.765LysGlu: 3.765 ± 0.017	1.663LysPhe: 1.663 ± 0.01	3.077LysGly: 3.077 ± 0.014	1.24LysHis: 1.24 ± 0.009	2.53LysIle: 2.53 ± 0.013	3.728LysLys: 3.728 ± 0.023	4.975LysLeu: 4.975 ± 0.02	1.295LysMet: 1.295 ± 0.009	1.955LysAsn: 1.955 ± 0.011	2.327LysPro: 2.327 ± 0.013	1.979LysGln: 1.979 ± 0.011	3.242LysArg: 3.242 ± 0.015	3.601LysSer: 3.601 ± 0.017	2.311LysThr: 2.311 ± 0.01	3.248LysVal: 3.248 ± 0.015	0.615LysTrp: 0.615 ± 0.008	1.352LysTyr: 1.352 ± 0.009	0.0LysXaa: 0.0 ± 0.0
Leu	8.026LeuAla: 8.026 ± 0.026	1.966LeuCys: 1.966 ± 0.012	5.114LeuAsp: 5.114 ± 0.017	5.788LeuGlu: 5.788 ± 0.023	3.513LeuPhe: 3.513 ± 0.016	5.98LeuGly: 5.98 ± 0.023	2.787LeuHis: 2.787 ± 0.015	3.692LeuIle: 3.692 ± 0.017	4.839LeuLys: 4.839 ± 0.02	10.739LeuLeu: 10.739 ± 0.032	2.044LeuMet: 2.044 ± 0.011	3.06LeuAsn: 3.06 ± 0.014	5.933LeuPro: 5.933 ± 0.023	4.182LeuGln: 4.182 ± 0.019	6.317LeuArg: 6.317 ± 0.024	8.004LeuSer: 8.004 ± 0.028	4.182LeuThr: 4.182 ± 0.018	6.528LeuVal: 6.528 ± 0.023	1.148LeuTrp: 1.148 ± 0.009	2.294LeuTyr: 2.294 ± 0.012	0.0LeuXaa: 0.0 ± 0.0
Met	2.624MetAla: 2.624 ± 0.013	0.366MetCys: 0.366 ± 0.004	1.496MetAsp: 1.496 ± 0.01	1.875MetGlu: 1.875 ± 0.01	0.793MetPhe: 0.793 ± 0.007	1.52MetGly: 1.52 ± 0.01	0.597MetHis: 0.597 ± 0.006	0.996MetIle: 0.996 ± 0.008	1.311MetLys: 1.311 ± 0.01	2.278MetLeu: 2.278 ± 0.013	0.679MetMet: 0.679 ± 0.006	0.833MetAsn: 0.833 ± 0.007	1.305MetPro: 1.305 ± 0.01	0.964MetGln: 0.964 ± 0.008	1.345MetArg: 1.345 ± 0.008	1.795MetSer: 1.795 ± 0.01	1.053MetThr: 1.053 ± 0.007	1.64MetVal: 1.64 ± 0.009	0.271MetTrp: 0.271 ± 0.004	0.585MetTyr: 0.585 ± 0.006	0.0MetXaa: 0.0 ± 0.0
Asn	2.554AsnAla: 2.554 ± 0.011	0.741AsnCys: 0.741 ± 0.008	1.748AsnAsp: 1.748 ± 0.012	1.899AsnGlu: 1.899 ± 0.01	1.431AsnPhe: 1.431 ± 0.008	2.828AsnGly: 2.828 ± 0.016	0.919AsnHis: 0.919 ± 0.008	1.981AsnIle: 1.981 ± 0.011	1.88AsnLys: 1.88 ± 0.011	3.637AsnLeu: 3.637 ± 0.018	1.006AsnMet: 1.006 ± 0.007	1.742AsnAsn: 1.742 ± 0.013	1.921AsnPro: 1.921 ± 0.011	1.288AsnGln: 1.288 ± 0.009	1.803AsnArg: 1.803 ± 0.01	2.953AsnSer: 2.953 ± 0.015	1.716AsnThr: 1.716 ± 0.01	2.294AsnVal: 2.294 ± 0.012	0.45AsnTrp: 0.45 ± 0.004	0.998AsnTyr: 0.998 ± 0.007	0.0AsnXaa: 0.0 ± 0.0
Pro	5.375ProAla: 5.375 ± 0.023	1.004ProCys: 1.004 ± 0.008	2.799ProAsp: 2.799 ± 0.014	3.26ProGlu: 3.26 ± 0.016	1.908ProPhe: 1.908 ± 0.011	3.41ProGly: 3.41 ± 0.018	1.261ProHis: 1.261 ± 0.01	1.931ProIle: 1.931 ± 0.01	2.294ProLys: 2.294 ± 0.012	4.682ProLeu: 4.682 ± 0.017	1.104ProMet: 1.104 ± 0.008	1.854ProAsn: 1.854 ± 0.009	5.64ProPro: 5.64 ± 0.038	1.846ProGln: 1.846 ± 0.013	3.548ProArg: 3.548 ± 0.018	5.643ProSer: 5.643 ± 0.022	2.801ProThr: 2.801 ± 0.013	3.515ProVal: 3.515 ± 0.017	0.705ProTrp: 0.705 ± 0.007	1.247ProTyr: 1.247 ± 0.009	0.0ProXaa: 0.0 ± 0.0
Gln	2.735GlnAla: 2.735 ± 0.014	0.603GlnCys: 0.603 ± 0.006	1.708GlnAsp: 1.708 ± 0.01	2.35GlnGlu: 2.35 ± 0.013	1.175GlnPhe: 1.175 ± 0.008	2.255GlnGly: 2.255 ± 0.011	1.051GlnHis: 1.051 ± 0.008	1.66GlnIle: 1.66 ± 0.009	1.938GlnLys: 1.938 ± 0.012	3.528GlnLeu: 3.528 ± 0.016	0.943GlnMet: 0.943 ± 0.006	1.388GlnAsn: 1.388 ± 0.01	1.936GlnPro: 1.936 ± 0.01	2.35GlnGln: 2.35 ± 0.025	2.297GlnArg: 2.297 ± 0.011	2.593GlnSer: 2.593 ± 0.014	1.535GlnThr: 1.535 ± 0.01	2.243GlnVal: 2.243 ± 0.012	0.448GlnTrp: 0.448 ± 0.005	0.906GlnTyr: 0.906 ± 0.007	0.0GlnXaa: 0.0 ± 0.0
Arg	4.987ArgAla: 4.987 ± 0.019	1.366ArgCys: 1.366 ± 0.009	2.961ArgAsp: 2.961 ± 0.014	3.441ArgGlu: 3.441 ± 0.018	2.26ArgPhe: 2.26 ± 0.01	4.202ArgGly: 4.202 ± 0.022	1.7ArgHis: 1.7 ± 0.01	2.654ArgIle: 2.654 ± 0.014	3.445ArgLys: 3.445 ± 0.016	6.015ArgLeu: 6.015 ± 0.024	1.482ArgMet: 1.482 ± 0.011	2.212ArgAsn: 2.212 ± 0.013	3.403ArgPro: 3.403 ± 0.018	2.147ArgGln: 2.147 ± 0.011	6.373ArgArg: 6.373 ± 0.036	5.108ArgSer: 5.108 ± 0.021	2.781ArgThr: 2.781 ± 0.014	3.679ArgVal: 3.679 ± 0.018	0.991ArgTrp: 0.991 ± 0.008	1.548ArgTyr: 1.548 ± 0.009	0.0ArgXaa: 0.0 ± 0.0
Ser	6.701SerAla: 6.701 ± 0.023	1.856SerCys: 1.856 ± 0.013	4.15SerAsp: 4.15 ± 0.017	4.215SerGlu: 4.215 ± 0.017	3.426SerPhe: 3.426 ± 0.016	6.126SerGly: 6.126 ± 0.024	1.873SerHis: 1.873 ± 0.011	3.69SerIle: 3.69 ± 0.015	3.987SerLys: 3.987 ± 0.016	7.968SerLeu: 7.968 ± 0.029	2.07SerMet: 2.07 ± 0.011	3.137SerAsn: 3.137 ± 0.016	5.149SerPro: 5.149 ± 0.026	2.611SerGln: 2.611 ± 0.015	4.97SerArg: 4.97 ± 0.021	10.792SerSer: 10.792 ± 0.04	4.632SerThr: 4.632 ± 0.018	5.104SerVal: 5.104 ± 0.018	1.173SerTrp: 1.173 ± 0.007	2.121SerTyr: 2.121 ± 0.012	0.0SerXaa: 0.0 ± 0.0
Thr	4.287ThrAla: 4.287 ± 0.017	0.971ThrCys: 0.971 ± 0.008	2.365ThrAsp: 2.365 ± 0.012	2.698ThrGlu: 2.698 ± 0.015	1.777ThrPhe: 1.777 ± 0.009	3.556ThrGly: 3.556 ± 0.015	0.992ThrHis: 0.992 ± 0.007	2.285ThrIle: 2.285 ± 0.012	2.322ThrLys: 2.322 ± 0.012	4.284ThrLeu: 4.284 ± 0.016	1.219ThrMet: 1.219 ± 0.009	1.764ThrAsn: 1.764 ± 0.011	2.844ThrPro: 2.844 ± 0.015	1.396ThrGln: 1.396 ± 0.009	2.649ThrArg: 2.649 ± 0.012	4.39ThrSer: 4.39 ± 0.018	3.008ThrThr: 3.008 ± 0.018	3.474ThrVal: 3.474 ± 0.015	0.617ThrTrp: 0.617 ± 0.006	1.258ThrTyr: 1.258 ± 0.009	0.0ThrXaa: 0.0 ± 0.0
Val	6.148ValAla: 6.148 ± 0.023	1.242ValCys: 1.242 ± 0.008	3.827ValAsp: 3.827 ± 0.016	4.043ValGlu: 4.043 ± 0.017	2.496ValPhe: 2.496 ± 0.015	4.318ValGly: 4.318 ± 0.017	1.678ValHis: 1.678 ± 0.01	2.914ValIle: 2.914 ± 0.015	3.167ValLys: 3.167 ± 0.015	6.735ValLeu: 6.735 ± 0.025	1.528ValMet: 1.528 ± 0.008	2.171ValAsn: 2.171 ± 0.013	3.758ValPro: 3.758 ± 0.014	2.35ValGln: 2.35 ± 0.012	3.736ValArg: 3.736 ± 0.014	5.202ValSer: 5.202 ± 0.017	3.213ValThr: 3.213 ± 0.015	5.24ValVal: 5.24 ± 0.022	0.804ValTrp: 0.804 ± 0.007	1.796ValTyr: 1.796 ± 0.011	0.0ValXaa: 0.0 ± 0.0
Trp	0.949TrpAla: 0.949 ± 0.008	0.275TrpCys: 0.275 ± 0.004	0.687TrpAsp: 0.687 ± 0.007	0.722TrpGlu: 0.722 ± 0.007	0.493TrpPhe: 0.493 ± 0.005	0.739TrpGly: 0.739 ± 0.006	0.338TrpHis: 0.338 ± 0.005	0.622TrpIle: 0.622 ± 0.007	0.777TrpLys: 0.777 ± 0.007	1.264TrpLeu: 1.264 ± 0.009	0.355TrpMet: 0.355 ± 0.005	0.574TrpAsn: 0.574 ± 0.007	0.622TrpPro: 0.622 ± 0.006	0.461TrpGln: 0.461 ± 0.005	1.056TrpArg: 1.056 ± 0.009	1.031TrpSer: 1.031 ± 0.009	0.664TrpThr: 0.664 ± 0.007	0.79TrpVal: 0.79 ± 0.008	0.251TrpTrp: 0.251 ± 0.004	0.326TrpTyr: 0.326 ± 0.004	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.867TyrAla: 1.867 ± 0.01	0.611TyrCys: 0.611 ± 0.006	1.445TyrAsp: 1.445 ± 0.009	1.37TyrGlu: 1.37 ± 0.009	1.106TyrPhe: 1.106 ± 0.007	1.95TyrGly: 1.95 ± 0.013	0.691TyrHis: 0.691 ± 0.007	1.258TyrIle: 1.258 ± 0.009	1.226TyrLys: 1.226 ± 0.009	2.581TyrLeu: 2.581 ± 0.013	0.71TyrMet: 0.71 ± 0.006	1.071TyrAsn: 1.071 ± 0.008	1.179TyrPro: 1.179 ± 0.008	0.855TyrGln: 0.855 ± 0.007	1.469TyrArg: 1.469 ± 0.01	2.011TyrSer: 2.011 ± 0.011	1.229TyrThr: 1.229 ± 0.008	1.641TyrVal: 1.641 ± 0.01	0.374TyrTrp: 0.374 ± 0.005	0.891TyrTyr: 0.891 ± 0.007	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 44786 proteins (18330814 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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