Dyadobacter sp. Leaf189

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cytophagaceae; Dyadobacter; unclassified Dyadobacter

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4994 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q5TMC1|A0A0Q5TMC1_9BACT Dihydrodipicolinate synthetase OS=Dyadobacter sp. Leaf189 OX=1736295 GN=ASG33_10985 PE=3 SV=1
MM1 pKa = 6.97TQDD4 pKa = 2.89NCFLLGYY11 pKa = 9.76IVRR14 pKa = 11.84THH16 pKa = 5.52GTAGNVVFYY25 pKa = 11.13LDD27 pKa = 2.99VDD29 pKa = 3.99YY30 pKa = 11.24PDD32 pKa = 5.54DD33 pKa = 4.56YY34 pKa = 11.98EE35 pKa = 5.03DD36 pKa = 4.87LEE38 pKa = 4.56TVYY41 pKa = 11.12VEE43 pKa = 4.13IKK45 pKa = 10.68GDD47 pKa = 3.58LVPYY51 pKa = 10.04FIEE54 pKa = 5.67DD55 pKa = 3.52INIQKK60 pKa = 10.05QGNAIVALEE69 pKa = 4.37EE70 pKa = 4.22VNTMAKK76 pKa = 10.03AQALVGSSLYY86 pKa = 10.9LPLEE90 pKa = 4.43DD91 pKa = 5.47LEE93 pKa = 4.42EE94 pKa = 5.17LGGEE98 pKa = 3.65NFYY101 pKa = 10.99YY102 pKa = 10.64HH103 pKa = 7.36EE104 pKa = 4.52IKK106 pKa = 10.59GYY108 pKa = 7.4TVIDD112 pKa = 3.52QTAGEE117 pKa = 4.17LGIVRR122 pKa = 11.84EE123 pKa = 4.42VYY125 pKa = 10.6SLNGQDD131 pKa = 5.35LIAMDD136 pKa = 4.0YY137 pKa = 10.9EE138 pKa = 4.44GTEE141 pKa = 4.0VLIPTANEE149 pKa = 3.37IVQRR153 pKa = 11.84ADD155 pKa = 3.31KK156 pKa = 8.66EE157 pKa = 4.83TKK159 pKa = 9.76QLLVNLPEE167 pKa = 4.76GLLEE171 pKa = 4.34VYY173 pKa = 10.36LSKK176 pKa = 11.23DD177 pKa = 3.03SDD179 pKa = 4.09SEE181 pKa = 4.4NIPDD185 pKa = 5.14DD186 pKa = 4.21ADD188 pKa = 3.21

Molecular weight: 21.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q5T9I9|A0A0Q5T9I9_9BACT Peptidase_M56 domain-containing protein OS=Dyadobacter sp. Leaf189 OX=1736295 GN=ASG33_20130 PE=4 SV=1
MM1 pKa = 7.56FPILLIAHH9 pKa = 7.28SITRR13 pKa = 11.84WFVVTTLSIAIYY25 pKa = 9.92RR26 pKa = 11.84GIRR29 pKa = 11.84GWRR32 pKa = 11.84GAKK35 pKa = 9.76EE36 pKa = 3.72FTRR39 pKa = 11.84RR40 pKa = 11.84DD41 pKa = 3.09DD42 pKa = 3.8SLRR45 pKa = 11.84HH46 pKa = 4.41GTATFSHH53 pKa = 5.87IQMMIGYY60 pKa = 8.81LLYY63 pKa = 10.66FNSPFISYY71 pKa = 9.41FRR73 pKa = 11.84SHH75 pKa = 6.6FKK77 pKa = 10.5EE78 pKa = 4.73AIQHH82 pKa = 5.81FDD84 pKa = 3.3FVFFGILHH92 pKa = 5.7ITLMTIAVIIITIGSSAAKK111 pKa = 9.97RR112 pKa = 11.84QQTDD116 pKa = 3.19LAKK119 pKa = 10.41FRR121 pKa = 11.84IMTICFIVAAIIIFIAIPWPFSPLATRR148 pKa = 11.84PYY150 pKa = 11.05LRR152 pKa = 11.84IFF154 pKa = 3.68

Molecular weight: 17.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4994

0

4994

1823744

55

2385

365.2

40.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.902 ± 0.031

0.713 ± 0.01

5.282 ± 0.023

6.075 ± 0.03

4.92 ± 0.024

7.135 ± 0.034

1.821 ± 0.015

6.599 ± 0.029

6.282 ± 0.028

9.469 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.275 ± 0.013

5.093 ± 0.029

4.1 ± 0.02

3.771 ± 0.018

4.581 ± 0.021

6.545 ± 0.028

5.655 ± 0.028

6.645 ± 0.026

1.276 ± 0.012

3.861 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski