Amino acid dipepetide frequency for Microbacterium sp. SYP-A9085

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
23.478AlaAla: 23.478 ± 0.267
0.766AlaCys: 0.766 ± 0.037
9.191AlaAsp: 9.191 ± 0.119
7.173AlaGlu: 7.173 ± 0.12
3.987AlaPhe: 3.987 ± 0.067
13.116AlaGly: 13.116 ± 0.14
2.993AlaHis: 2.993 ± 0.056
5.654AlaIle: 5.654 ± 0.081
2.698AlaLys: 2.698 ± 0.071
14.51AlaLeu: 14.51 ± 0.177
2.845AlaMet: 2.845 ± 0.057
2.084AlaAsn: 2.084 ± 0.053
6.722AlaPro: 6.722 ± 0.121
4.635AlaGln: 4.635 ± 0.092
10.41AlaArg: 10.41 ± 0.132
6.972AlaSer: 6.972 ± 0.111
7.497AlaThr: 7.497 ± 0.101
12.984AlaVal: 12.984 ± 0.156
2.02AlaTrp: 2.02 ± 0.05
2.528AlaTyr: 2.528 ± 0.051
0.0AlaXaa: 0.0 ± 0.0
Cys
0.782CysAla: 0.782 ± 0.033
0.047CysCys: 0.047 ± 0.007
0.363CysAsp: 0.363 ± 0.024
0.257CysGlu: 0.257 ± 0.018
0.18CysPhe: 0.18 ± 0.014
0.618CysGly: 0.618 ± 0.03
0.13CysHis: 0.13 ± 0.014
0.195CysIle: 0.195 ± 0.016
0.076CysLys: 0.076 ± 0.011
0.455CysLeu: 0.455 ± 0.025
0.09CysMet: 0.09 ± 0.01
0.098CysAsn: 0.098 ± 0.012
0.298CysPro: 0.298 ± 0.018
0.128CysGln: 0.128 ± 0.013
0.387CysArg: 0.387 ± 0.024
0.304CysSer: 0.304 ± 0.022
0.358CysThr: 0.358 ± 0.023
0.482CysVal: 0.482 ± 0.025
0.084CysTrp: 0.084 ± 0.01
0.088CysTyr: 0.088 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
10.363AspAla: 10.363 ± 0.127
0.256AspCys: 0.256 ± 0.017
4.324AspAsp: 4.324 ± 0.095
4.231AspGlu: 4.231 ± 0.077
1.566AspPhe: 1.566 ± 0.044
6.276AspGly: 6.276 ± 0.102
1.202AspHis: 1.202 ± 0.041
2.581AspIle: 2.581 ± 0.062
0.977AspLys: 0.977 ± 0.039
6.02AspLeu: 6.02 ± 0.095
0.821AspMet: 0.821 ± 0.032
0.863AspAsn: 0.863 ± 0.036
4.355AspPro: 4.355 ± 0.069
1.508AspGln: 1.508 ± 0.044
4.795AspArg: 4.795 ± 0.073
2.201AspSer: 2.201 ± 0.047
3.008AspThr: 3.008 ± 0.056
6.08AspVal: 6.08 ± 0.109
0.873AspTrp: 0.873 ± 0.035
1.175AspTyr: 1.175 ± 0.037
0.0AspXaa: 0.0 ± 0.0
Glu
5.993GluAla: 5.993 ± 0.097
0.237GluCys: 0.237 ± 0.016
2.779GluAsp: 2.779 ± 0.065
2.273GluGlu: 2.273 ± 0.067
1.577GluPhe: 1.577 ± 0.042
3.445GluGly: 3.445 ± 0.062
1.386GluHis: 1.386 ± 0.044
2.563GluIle: 2.563 ± 0.059
1.23GluLys: 1.23 ± 0.043
5.776GluLeu: 5.776 ± 0.102
0.848GluMet: 0.848 ± 0.038
1.074GluAsn: 1.074 ± 0.033
2.73GluPro: 2.73 ± 0.073
2.119GluGln: 2.119 ± 0.058
4.854GluArg: 4.854 ± 0.097
2.232GluSer: 2.232 ± 0.055
2.529GluThr: 2.529 ± 0.064
4.002GluVal: 4.002 ± 0.074
0.72GluTrp: 0.72 ± 0.025
1.073GluTyr: 1.073 ± 0.032
0.0GluXaa: 0.0 ± 0.0
Phe
4.417PheAla: 4.417 ± 0.076
0.172PheCys: 0.172 ± 0.015
2.282PheAsp: 2.282 ± 0.055
1.489PheGlu: 1.489 ± 0.045
1.016PhePhe: 1.016 ± 0.042
3.334PheGly: 3.334 ± 0.064
0.59PheHis: 0.59 ± 0.027
1.123PheIle: 1.123 ± 0.04
0.416PheLys: 0.416 ± 0.025
2.739PheLeu: 2.739 ± 0.054
0.447PheMet: 0.447 ± 0.024
0.563PheAsn: 0.563 ± 0.026
1.413PhePro: 1.413 ± 0.039
0.786PheGln: 0.786 ± 0.033
1.86PheArg: 1.86 ± 0.05
1.589PheSer: 1.589 ± 0.044
2.215PheThr: 2.215 ± 0.053
2.721PheVal: 2.721 ± 0.063
0.455PheTrp: 0.455 ± 0.022
0.638PheTyr: 0.638 ± 0.025
0.0PheXaa: 0.0 ± 0.0
Gly
11.364GlyAla: 11.364 ± 0.111
0.596GlyCys: 0.596 ± 0.027
5.209GlyAsp: 5.209 ± 0.086
4.305GlyGlu: 4.305 ± 0.077
3.208GlyPhe: 3.208 ± 0.07
7.961GlyGly: 7.961 ± 0.12
1.88GlyHis: 1.88 ± 0.043
4.984GlyIle: 4.984 ± 0.078
2.109GlyLys: 2.109 ± 0.052
8.447GlyLeu: 8.447 ± 0.111
2.288GlyMet: 2.288 ± 0.055
1.526GlyAsn: 1.526 ± 0.044
4.122GlyPro: 4.122 ± 0.077
2.543GlyGln: 2.543 ± 0.06
6.976GlyArg: 6.976 ± 0.092
4.889GlySer: 4.889 ± 0.084
5.79GlyThr: 5.79 ± 0.096
8.149GlyVal: 8.149 ± 0.115
1.607GlyTrp: 1.607 ± 0.046
2.238GlyTyr: 2.238 ± 0.047
0.0GlyXaa: 0.0 ± 0.0
His
2.782HisAla: 2.782 ± 0.062
0.118HisCys: 0.118 ± 0.011
1.415HisAsp: 1.415 ± 0.042
1.188HisGlu: 1.188 ± 0.041
0.548HisPhe: 0.548 ± 0.028
2.126HisGly: 2.126 ± 0.052
0.548HisHis: 0.548 ± 0.03
0.824HisIle: 0.824 ± 0.031
0.29HisLys: 0.29 ± 0.019
2.005HisLeu: 2.005 ± 0.048
0.352HisMet: 0.352 ± 0.02
0.381HisAsn: 0.381 ± 0.023
1.714HisPro: 1.714 ± 0.046
0.469HisGln: 0.469 ± 0.025
1.853HisArg: 1.853 ± 0.049
0.913HisSer: 0.913 ± 0.034
1.091HisThr: 1.091 ± 0.035
1.822HisVal: 1.822 ± 0.049
0.295HisTrp: 0.295 ± 0.019
0.395HisTyr: 0.395 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
7.06IleAla: 7.06 ± 0.086
0.237IleCys: 0.237 ± 0.015
3.299IleAsp: 3.299 ± 0.062
2.463IleGlu: 2.463 ± 0.05
0.997IlePhe: 0.997 ± 0.037
4.656IleGly: 4.656 ± 0.084
0.764IleHis: 0.764 ± 0.035
1.655IleIle: 1.655 ± 0.05
0.723IleLys: 0.723 ± 0.034
4.016IleLeu: 4.016 ± 0.067
0.638IleMet: 0.638 ± 0.028
0.805IleAsn: 0.805 ± 0.033
2.673IlePro: 2.673 ± 0.057
1.058IleGln: 1.058 ± 0.034
3.013IleArg: 3.013 ± 0.066
2.062IleSer: 2.062 ± 0.049
2.736IleThr: 2.736 ± 0.06
4.726IleVal: 4.726 ± 0.084
0.469IleTrp: 0.469 ± 0.024
0.735IleTyr: 0.735 ± 0.033
0.0IleXaa: 0.0 ± 0.0
Lys
2.493LysAla: 2.493 ± 0.069
0.069LysCys: 0.069 ± 0.008
1.202LysAsp: 1.202 ± 0.045
0.786LysGlu: 0.786 ± 0.033
0.435LysPhe: 0.435 ± 0.025
1.58LysGly: 1.58 ± 0.056
0.413LysHis: 0.413 ± 0.019
0.928LysIle: 0.928 ± 0.042
0.708LysLys: 0.708 ± 0.044
1.722LysLeu: 1.722 ± 0.057
0.364LysMet: 0.364 ± 0.021
0.463LysAsn: 0.463 ± 0.027
1.112LysPro: 1.112 ± 0.038
0.655LysGln: 0.655 ± 0.031
1.369LysArg: 1.369 ± 0.044
0.988LysSer: 0.988 ± 0.042
1.377LysThr: 1.377 ± 0.046
1.62LysVal: 1.62 ± 0.057
0.24LysTrp: 0.24 ± 0.018
0.441LysTyr: 0.441 ± 0.026
0.0LysXaa: 0.0 ± 0.0
Leu
14.743LeuAla: 14.743 ± 0.163
0.568LeuCys: 0.568 ± 0.024
6.466LeuAsp: 6.466 ± 0.098
4.665LeuGlu: 4.665 ± 0.085
2.836LeuPhe: 2.836 ± 0.062
8.822LeuGly: 8.822 ± 0.116
1.973LeuHis: 1.973 ± 0.051
4.245LeuIle: 4.245 ± 0.088
1.734LeuLys: 1.734 ± 0.053
9.455LeuLeu: 9.455 ± 0.114
1.651LeuMet: 1.651 ± 0.046
1.701LeuAsn: 1.701 ± 0.047
5.428LeuPro: 5.428 ± 0.089
2.775LeuGln: 2.775 ± 0.058
7.967LeuArg: 7.967 ± 0.104
5.327LeuSer: 5.327 ± 0.07
6.247LeuThr: 6.247 ± 0.089
9.249LeuVal: 9.249 ± 0.13
1.296LeuTrp: 1.296 ± 0.038
1.734LeuTyr: 1.734 ± 0.051
0.0LeuXaa: 0.0 ± 0.0
Met
2.245MetAla: 2.245 ± 0.048
0.112MetCys: 0.112 ± 0.012
0.993MetAsp: 0.993 ± 0.035
0.586MetGlu: 0.586 ± 0.026
0.536MetPhe: 0.536 ± 0.026
1.371MetGly: 1.371 ± 0.039
0.384MetHis: 0.384 ± 0.024
0.837MetIle: 0.837 ± 0.03
0.412MetLys: 0.412 ± 0.027
2.022MetLeu: 2.022 ± 0.049
0.376MetMet: 0.376 ± 0.02
0.465MetAsn: 0.465 ± 0.021
1.246MetPro: 1.246 ± 0.033
0.547MetGln: 0.547 ± 0.026
1.575MetArg: 1.575 ± 0.046
1.477MetSer: 1.477 ± 0.041
1.77MetThr: 1.77 ± 0.042
1.394MetVal: 1.394 ± 0.043
0.21MetTrp: 0.21 ± 0.017
0.307MetTyr: 0.307 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
2.389AsnAla: 2.389 ± 0.058
0.106AsnCys: 0.106 ± 0.011
0.985AsnAsp: 0.985 ± 0.032
0.812AsnGlu: 0.812 ± 0.028
0.524AsnPhe: 0.524 ± 0.027
1.68AsnGly: 1.68 ± 0.056
0.328AsnHis: 0.328 ± 0.019
0.791AsnIle: 0.791 ± 0.034
0.337AsnLys: 0.337 ± 0.023
1.827AsnLeu: 1.827 ± 0.039
0.307AsnMet: 0.307 ± 0.018
0.389AsnAsn: 0.389 ± 0.024
1.451AsnPro: 1.451 ± 0.044
0.498AsnGln: 0.498 ± 0.024
1.201AsnArg: 1.201 ± 0.039
0.823AsnSer: 0.823 ± 0.034
1.117AsnThr: 1.117 ± 0.037
1.536AsnVal: 1.536 ± 0.039
0.265AsnTrp: 0.265 ± 0.017
0.389AsnTyr: 0.389 ± 0.021
0.0AsnXaa: 0.0 ± 0.0
Pro
7.996ProAla: 7.996 ± 0.112
0.192ProCys: 0.192 ± 0.014
4.263ProAsp: 4.263 ± 0.079
3.208ProGlu: 3.208 ± 0.067
1.726ProPhe: 1.726 ± 0.042
5.16ProGly: 5.16 ± 0.077
1.241ProHis: 1.241 ± 0.038
2.042ProIle: 2.042 ± 0.049
0.979ProLys: 0.979 ± 0.041
4.962ProLeu: 4.962 ± 0.085
0.954ProMet: 0.954 ± 0.033
0.909ProAsn: 0.909 ± 0.032
2.406ProPro: 2.406 ± 0.062
1.617ProGln: 1.617 ± 0.045
3.74ProArg: 3.74 ± 0.075
2.922ProSer: 2.922 ± 0.058
3.29ProThr: 3.29 ± 0.072
5.148ProVal: 5.148 ± 0.087
0.892ProTrp: 0.892 ± 0.036
1.103ProTyr: 1.103 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
3.888GlnAla: 3.888 ± 0.063
0.121GlnCys: 0.121 ± 0.011
1.44GlnAsp: 1.44 ± 0.041
1.29GlnGlu: 1.29 ± 0.043
0.828GlnPhe: 0.828 ± 0.031
2.119GlnGly: 2.119 ± 0.046
0.672GlnHis: 0.672 ± 0.028
1.468GlnIle: 1.468 ± 0.047
0.677GlnLys: 0.677 ± 0.031
3.346GlnLeu: 3.346 ± 0.064
0.554GlnMet: 0.554 ± 0.024
0.631GlnAsn: 0.631 ± 0.026
1.448GlnPro: 1.448 ± 0.047
1.15GlnGln: 1.15 ± 0.042
2.7GlnArg: 2.7 ± 0.068
1.461GlnSer: 1.461 ± 0.042
1.737GlnThr: 1.737 ± 0.044
2.466GlnVal: 2.466 ± 0.049
0.483GlnTrp: 0.483 ± 0.024
0.587GlnTyr: 0.587 ± 0.025
0.0GlnXaa: 0.0 ± 0.0
Arg
10.021ArgAla: 10.021 ± 0.135
0.384ArgCys: 0.384 ± 0.023
4.781ArgAsp: 4.781 ± 0.077
4.17ArgGlu: 4.17 ± 0.08
2.472ArgPhe: 2.472 ± 0.051
5.884ArgGly: 5.884 ± 0.088
1.817ArgHis: 1.817 ± 0.054
4.014ArgIle: 4.014 ± 0.057
1.322ArgLys: 1.322 ± 0.043
7.821ArgLeu: 7.821 ± 0.102
1.995ArgMet: 1.995 ± 0.047
1.332ArgAsn: 1.332 ± 0.04
3.956ArgPro: 3.956 ± 0.078
2.162ArgGln: 2.162 ± 0.057
7.527ArgArg: 7.527 ± 0.118
3.939ArgSer: 3.939 ± 0.075
4.845ArgThr: 4.845 ± 0.082
6.549ArgVal: 6.549 ± 0.091
1.212ArgTrp: 1.212 ± 0.043
1.65ArgTyr: 1.65 ± 0.047
0.0ArgXaa: 0.0 ± 0.0
Ser
6.729SerAla: 6.729 ± 0.108
0.279SerCys: 0.279 ± 0.021
2.762SerAsp: 2.762 ± 0.063
2.101SerGlu: 2.101 ± 0.049
1.801SerPhe: 1.801 ± 0.05
5.496SerGly: 5.496 ± 0.094
0.974SerHis: 0.974 ± 0.036
2.389SerIle: 2.389 ± 0.054
0.901SerLys: 0.901 ± 0.036
4.868SerLeu: 4.868 ± 0.083
1.057SerMet: 1.057 ± 0.037
0.894SerAsn: 0.894 ± 0.033
2.864SerPro: 2.864 ± 0.058
1.315SerGln: 1.315 ± 0.035
3.684SerArg: 3.684 ± 0.069
2.752SerSer: 2.752 ± 0.067
3.384SerThr: 3.384 ± 0.079
4.406SerVal: 4.406 ± 0.074
0.791SerTrp: 0.791 ± 0.031
1.067SerTyr: 1.067 ± 0.035
0.0SerXaa: 0.0 ± 0.0
Thr
8.643ThrAla: 8.643 ± 0.121
0.341ThrCys: 0.341 ± 0.022
3.461ThrAsp: 3.461 ± 0.06
2.516ThrGlu: 2.516 ± 0.058
1.797ThrPhe: 1.797 ± 0.046
6.202ThrGly: 6.202 ± 0.097
1.214ThrHis: 1.214 ± 0.037
2.697ThrIle: 2.697 ± 0.052
1.137ThrLys: 1.137 ± 0.04
5.832ThrLeu: 5.832 ± 0.077
1.097ThrMet: 1.097 ± 0.031
1.102ThrAsn: 1.102 ± 0.041
3.997ThrPro: 3.997 ± 0.08
1.551ThrGln: 1.551 ± 0.041
4.025ThrArg: 4.025 ± 0.065
3.21ThrSer: 3.21 ± 0.07
3.658ThrThr: 3.658 ± 0.086
6.178ThrVal: 6.178 ± 0.098
0.884ThrTrp: 0.884 ± 0.032
1.137ThrTyr: 1.137 ± 0.041
0.0ThrXaa: 0.0 ± 0.0
Val
12.672ValAla: 12.672 ± 0.139
0.602ValCys: 0.602 ± 0.027
6.144ValAsp: 6.144 ± 0.081
4.275ValGlu: 4.275 ± 0.086
3.021ValPhe: 3.021 ± 0.056
7.325ValGly: 7.325 ± 0.103
1.935ValHis: 1.935 ± 0.046
4.377ValIle: 4.377 ± 0.078
1.626ValLys: 1.626 ± 0.053
9.307ValLeu: 9.307 ± 0.127
1.539ValMet: 1.539 ± 0.039
1.715ValAsn: 1.715 ± 0.043
4.973ValPro: 4.973 ± 0.077
2.449ValGln: 2.449 ± 0.055
6.852ValArg: 6.852 ± 0.093
4.687ValSer: 4.687 ± 0.082
5.918ValThr: 5.918 ± 0.091
9.275ValVal: 9.275 ± 0.13
1.174ValTrp: 1.174 ± 0.039
1.637ValTyr: 1.637 ± 0.037
0.0ValXaa: 0.0 ± 0.0
Trp
1.653TrpAla: 1.653 ± 0.046
0.098TrpCys: 0.098 ± 0.011
0.829TrpAsp: 0.829 ± 0.029
0.627TrpGlu: 0.627 ± 0.029
0.562TrpPhe: 0.562 ± 0.026
1.042TrpGly: 1.042 ± 0.032
0.365TrpHis: 0.365 ± 0.022
0.686TrpIle: 0.686 ± 0.03
0.257TrpLys: 0.257 ± 0.019
1.67TrpLeu: 1.67 ± 0.045
0.398TrpMet: 0.398 ± 0.022
0.365TrpAsn: 0.365 ± 0.02
0.731TrpPro: 0.731 ± 0.028
0.53TrpGln: 0.53 ± 0.028
1.316TrpArg: 1.316 ± 0.04
0.805TrpSer: 0.805 ± 0.03
0.903TrpThr: 0.903 ± 0.03
1.152TrpVal: 1.152 ± 0.04
0.331TrpTrp: 0.331 ± 0.02
0.266TrpTyr: 0.266 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.553TyrAla: 2.553 ± 0.041
0.119TyrCys: 0.119 ± 0.012
1.286TyrAsp: 1.286 ± 0.039
1.023TyrGlu: 1.023 ± 0.038
0.649TyrPhe: 0.649 ± 0.029
1.977TyrGly: 1.977 ± 0.052
0.32TyrHis: 0.32 ± 0.021
0.733TyrIle: 0.733 ± 0.027
0.355TyrLys: 0.355 ± 0.025
2.083TyrLeu: 2.083 ± 0.053
0.245TyrMet: 0.245 ± 0.016
0.395TyrAsn: 0.395 ± 0.023
1.112TyrPro: 1.112 ± 0.04
0.571TyrGln: 0.571 ± 0.026
1.749TyrArg: 1.749 ± 0.048
0.958TyrSer: 0.958 ± 0.033
1.211TyrThr: 1.211 ± 0.041
1.563TyrVal: 1.563 ± 0.04
0.285TyrTrp: 0.285 ± 0.017
0.435TyrTyr: 0.435 ± 0.024
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2676 proteins (890392 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski