Opitutaceae bacterium TAV5

Taxonomy: cellular organisms; Bacteria; PVC group; Verrucomicrobia; Opitutae; Opitutales; Opitutaceae; unclassified Opitutaceae

Average proteome isoelectric point is 7.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6011 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W0J4S4|W0J4S4_9BACT MFS transporter OS=Opitutaceae bacterium TAV5 OX=794903 GN=OPIT5_17235 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 10.31RR3 pKa = 11.84ISCLLLALASCASLPASVLITGFGSDD29 pKa = 4.3DD30 pKa = 3.7FFDD33 pKa = 4.53AGSEE37 pKa = 3.99LALTQTEE44 pKa = 4.44TTSRR48 pKa = 11.84FVGSTLTGSIVGTFATSVDD67 pKa = 2.88ISGYY71 pKa = 8.98TSALALTATVSDD83 pKa = 4.18INSSIADD90 pKa = 3.84FQLLLWDD97 pKa = 3.93GTDD100 pKa = 3.53DD101 pKa = 4.77GFYY104 pKa = 10.38GYY106 pKa = 10.16QGNWSQFVTGEE117 pKa = 4.05EE118 pKa = 4.33STVLLTAMAGQPDD131 pKa = 4.58TFDD134 pKa = 3.21FTAIQGLMFSFGSGADD150 pKa = 3.52GTIDD154 pKa = 3.55LTLNTLAAVPEE165 pKa = 4.28PATCAILAGLACLGYY180 pKa = 9.78IAAGGRR186 pKa = 11.84RR187 pKa = 11.84RR188 pKa = 11.84WYY190 pKa = 10.41

Molecular weight: 19.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W0J4N5|W0J4N5_9BACT Phosphoadenosine phosphosulfate reductase OS=Opitutaceae bacterium TAV5 OX=794903 GN=OPIT5_18790 PE=4 SV=1
MM1 pKa = 7.15QPTFRR6 pKa = 11.84PHH8 pKa = 5.47RR9 pKa = 11.84KK10 pKa = 9.07KK11 pKa = 10.33RR12 pKa = 11.84VRR14 pKa = 11.84KK15 pKa = 8.2IGFRR19 pKa = 11.84ARR21 pKa = 11.84MATRR25 pKa = 11.84GGRR28 pKa = 11.84KK29 pKa = 9.2VIASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.53GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LTVAA45 pKa = 4.08

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6011

0

6011

2136471

29

4525

355.4

38.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.074 ± 0.04

0.901 ± 0.011

5.398 ± 0.025

5.115 ± 0.029

3.877 ± 0.018

8.48 ± 0.041

2.22 ± 0.016

4.78 ± 0.026

3.053 ± 0.026

10.052 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.742 ± 0.016

3.019 ± 0.023

5.906 ± 0.029

2.926 ± 0.017

7.433 ± 0.039

5.815 ± 0.027

6.271 ± 0.042

6.812 ± 0.027

1.703 ± 0.018

2.423 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski