Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; Clostridium acetobutylicum

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3847 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q97K36|Q97K36_CLOAB Alpha-glucosidase OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=CA_C1085 PE=3 SV=1
MM1 pKa = 7.17QNDD4 pKa = 4.15NISQIKK10 pKa = 9.74SSTVEE15 pKa = 3.81VQQDD19 pKa = 3.55DD20 pKa = 4.15NRR22 pKa = 11.84CDD24 pKa = 3.54MDD26 pKa = 3.97TKK28 pKa = 10.82DD29 pKa = 5.22DD30 pKa = 3.94EE31 pKa = 4.72MNSVYY36 pKa = 10.84YY37 pKa = 10.15YY38 pKa = 11.25LL39 pKa = 5.48

Molecular weight: 4.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q97FP2|Q97FP2_CLOAB Possible maltodextrin glucosidase OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=CA_C2686 PE=3 SV=1
MM1 pKa = 7.21KK2 pKa = 9.63TRR4 pKa = 11.84LFIAVIIILSGAAMLSSQLGIIDD27 pKa = 3.57VRR29 pKa = 11.84NVSSVFWPLILIAVGAQRR47 pKa = 11.84LYY49 pKa = 10.95KK50 pKa = 10.08IGRR53 pKa = 11.84FNIGGAVMVLFGMIFEE69 pKa = 4.63IEE71 pKa = 4.1ALHH74 pKa = 5.8LVPYY78 pKa = 9.4SAEE81 pKa = 4.06VFIAPCMIIIVGISLLFPRR100 pKa = 11.84RR101 pKa = 11.84KK102 pKa = 10.08AFNRR106 pKa = 3.55

Molecular weight: 11.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3847

0

3847

1176635

27

2870

305.9

34.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.666 ± 0.04

1.2 ± 0.015

5.543 ± 0.031

6.776 ± 0.053

4.485 ± 0.031

6.331 ± 0.047

1.342 ± 0.014

9.622 ± 0.049

9.386 ± 0.047

8.657 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.586 ± 0.018

6.558 ± 0.041

2.725 ± 0.019

2.337 ± 0.027

3.348 ± 0.024

6.807 ± 0.039

4.97 ± 0.038

6.618 ± 0.036

0.741 ± 0.017

4.305 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski