Mosquito VEM virus SDBVL G

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus mosqi1; Mosquito associated gemycircularvirus 1

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F6KIE2|F6KIE2_9VIRU Replication-associated protein OS=Mosquito VEM virus SDBVL G OX=1034805 GN=Rep2 PE=4 SV=1
MM1 pKa = 7.63PKK3 pKa = 9.79SLKK6 pKa = 9.86MSLQNCLCDD15 pKa = 3.38SCTSAQNMMPTSSSGRR31 pKa = 11.84NNTPTEE37 pKa = 4.38EE38 pKa = 4.29YY39 pKa = 10.16ISMPFSIFEE48 pKa = 4.65DD49 pKa = 4.0LNSQPEE55 pKa = 4.4TNDD58 pKa = 2.89FGTLPGDD65 pKa = 3.77TPTSQGWDD73 pKa = 2.99ARR75 pKa = 11.84RR76 pKa = 11.84GRR78 pKa = 11.84RR79 pKa = 11.84TITPSRR85 pKa = 11.84TTTSSEE91 pKa = 3.78EE92 pKa = 3.88QPPVLRR98 pKa = 11.84RR99 pKa = 11.84EE100 pKa = 4.07GAALGEE106 pKa = 4.33INNMRR111 pKa = 11.84TGPTSVRR118 pKa = 11.84QFLRR122 pKa = 11.84RR123 pKa = 11.84FSLIEE128 pKa = 4.09SEE130 pKa = 4.55NVDD133 pKa = 3.42PGIWSVLSAILEE145 pKa = 4.0NMQIGTTVLCPSRR158 pKa = 11.84IQLRR162 pKa = 11.84RR163 pKa = 11.84STDD166 pKa = 3.34SHH168 pKa = 5.52WLITLRR174 pKa = 11.84STTGPLPTLEE184 pKa = 4.42EE185 pKa = 3.95MLLPGVYY192 pKa = 9.9HH193 pKa = 6.88PPDD196 pKa = 3.91PRR198 pKa = 11.84QDD200 pKa = 3.07

Molecular weight: 22.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F6KIE3|F6KIE3_9VIRU Coat protein OS=Mosquito VEM virus SDBVL G OX=1034805 GN=coat protein PE=4 SV=1
MM1 pKa = 7.42VYY3 pKa = 10.0RR4 pKa = 11.84RR5 pKa = 11.84SRR7 pKa = 11.84PTSYY11 pKa = 10.36RR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84TGRR17 pKa = 11.84YY18 pKa = 7.63APRR21 pKa = 11.84RR22 pKa = 11.84SIYY25 pKa = 8.8RR26 pKa = 11.84RR27 pKa = 11.84KK28 pKa = 9.75RR29 pKa = 11.84MVYY32 pKa = 9.18RR33 pKa = 11.84RR34 pKa = 11.84ANARR38 pKa = 11.84RR39 pKa = 11.84TRR41 pKa = 11.84TRR43 pKa = 11.84GLPVKK48 pKa = 10.37RR49 pKa = 11.84IRR51 pKa = 11.84NLTSRR56 pKa = 11.84KK57 pKa = 9.74KK58 pKa = 10.15QDD60 pKa = 3.66TMLSWAKK67 pKa = 7.57PTPYY71 pKa = 10.0TSGAVGSPLMTGIPNARR88 pKa = 11.84DD89 pKa = 3.51PGIQPYY95 pKa = 10.5VYY97 pKa = 9.15MYY99 pKa = 10.82QPTARR104 pKa = 11.84DD105 pKa = 3.66LEE107 pKa = 4.35RR108 pKa = 11.84VPGSVTNINDD118 pKa = 3.56SASRR122 pKa = 11.84TSSNCYY128 pKa = 10.08AVGLKK133 pKa = 10.23EE134 pKa = 4.32KK135 pKa = 10.46ISLIVNSNEE144 pKa = 2.97EE145 pKa = 4.24WLWRR149 pKa = 11.84RR150 pKa = 11.84IVFTFKK156 pKa = 9.82GTYY159 pKa = 9.86LVTPQAGFGGDD170 pKa = 3.74SNNVGAFYY178 pKa = 11.03DD179 pKa = 3.88EE180 pKa = 4.54TSVGFPRR187 pKa = 11.84AWTQLSGFTASPGADD202 pKa = 3.17TNALWLALRR211 pKa = 11.84SLLFKK216 pKa = 10.17GTYY219 pKa = 7.1QTDD222 pKa = 3.13WNDD225 pKa = 2.9FATAKK230 pKa = 10.54ADD232 pKa = 3.57NSRR235 pKa = 11.84LNILYY240 pKa = 9.81DD241 pKa = 3.52KK242 pKa = 10.28QVKK245 pKa = 8.59VQSPNDD251 pKa = 3.38NGTTRR256 pKa = 11.84TFNRR260 pKa = 11.84WHH262 pKa = 7.36PFRR265 pKa = 11.84KK266 pKa = 9.59RR267 pKa = 11.84LIYY270 pKa = 10.76NDD272 pKa = 4.2DD273 pKa = 3.53EE274 pKa = 4.88VGGGKK279 pKa = 9.48QVDD282 pKa = 3.53VGYY285 pKa = 11.0SSMHH289 pKa = 6.09RR290 pKa = 11.84ASMGDD295 pKa = 3.03AYY297 pKa = 11.02VFDD300 pKa = 4.3MFIPGHH306 pKa = 5.83NSEE309 pKa = 4.42QADD312 pKa = 4.0EE313 pKa = 4.87LEE315 pKa = 4.65VRR317 pKa = 11.84CSSTYY322 pKa = 9.76YY323 pKa = 8.97WHH325 pKa = 6.35EE326 pKa = 3.98TT327 pKa = 3.35

Molecular weight: 37.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

869

121

327

217.3

24.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.869 ± 1.033

1.496 ± 0.417

5.869 ± 0.589

5.409 ± 1.075

4.373 ± 0.702

7.48 ± 0.546

1.956 ± 0.547

4.373 ± 0.583

3.797 ± 0.84

6.904 ± 0.943

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.301 ± 0.64

4.488 ± 0.676

6.559 ± 0.907

2.877 ± 0.553

8.746 ± 1.265

8.055 ± 1.067

7.48 ± 1.141

5.524 ± 0.732

2.417 ± 0.326

4.028 ± 1.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski