Citrobacter phage vB_CroP_CrRp3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Molineuxvirinae; Vectrevirus; Citrobacter CrRp3

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K9VAU6|A0A2K9VAU6_9CAUD Uncharacterized protein OS=Citrobacter phage vB_CroP_CrRp3 OX=2079275 GN=CrRp3_cds36 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 9.95RR3 pKa = 11.84DD4 pKa = 3.27ANAYY8 pKa = 9.81YY9 pKa = 9.95EE10 pKa = 4.45LLAATVEE17 pKa = 4.25AFNEE21 pKa = 4.38RR22 pKa = 11.84IQYY25 pKa = 10.53DD26 pKa = 4.62GIRR29 pKa = 11.84EE30 pKa = 4.07DD31 pKa = 5.36DD32 pKa = 4.53DD33 pKa = 5.25YY34 pKa = 12.06SDD36 pKa = 4.31ALHH39 pKa = 6.31EE40 pKa = 4.45VVAGNVPHH48 pKa = 6.81YY49 pKa = 8.76YY50 pKa = 10.33HH51 pKa = 7.6EE52 pKa = 4.77IFTVMAADD60 pKa = 5.34GIDD63 pKa = 3.92IEE65 pKa = 4.96FDD67 pKa = 3.66DD68 pKa = 5.68AGLIPDD74 pKa = 4.31TKK76 pKa = 10.91DD77 pKa = 2.98VTKK80 pKa = 10.5ILQARR85 pKa = 11.84IYY87 pKa = 8.8EE88 pKa = 4.07ALYY91 pKa = 11.03NDD93 pKa = 3.99VLNDD97 pKa = 3.74SGVVWYY103 pKa = 9.9EE104 pKa = 3.89DD105 pKa = 3.71EE106 pKa = 4.44EE107 pKa = 4.49EE108 pKa = 4.28EE109 pKa = 4.66EE110 pKa = 4.31

Molecular weight: 12.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R4PAL4|A0A2R4PAL4_9CAUD Uncharacterized protein OS=Citrobacter phage vB_CroP_CrRp3 OX=2079275 GN=CrRp3_cds33 PE=4 SV=1
MM1 pKa = 7.32GLVVDD6 pKa = 5.11KK7 pKa = 11.03VIALVKK13 pKa = 9.89PLSAFLRR20 pKa = 11.84PFRR23 pKa = 11.84AIPEE27 pKa = 4.21RR28 pKa = 11.84LTGCAGVGYY37 pKa = 9.91LFVSPSGSTSFTPLALRR54 pKa = 11.84SLAHH58 pKa = 5.61VYY60 pKa = 10.39LRR62 pKa = 11.84LFLDD66 pKa = 4.32GG67 pKa = 4.91

Molecular weight: 7.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

12986

40

1267

245.0

27.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.264 ± 0.545

0.993 ± 0.147

6.107 ± 0.226

7.3 ± 0.33

3.604 ± 0.167

7.778 ± 0.289

2.033 ± 0.201

5.537 ± 0.267

6.476 ± 0.298

7.716 ± 0.238

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.119 ± 0.169

4.328 ± 0.241

3.488 ± 0.183

4.22 ± 0.293

5.467 ± 0.302

5.621 ± 0.323

5.46 ± 0.352

6.861 ± 0.265

1.309 ± 0.123

3.319 ± 0.193
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski