Hyphomonas jannaschiana VP2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomonadales; Hyphomonadaceae; Hyphomonas; Hyphomonas jannaschiana

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3505 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A059FJU2|A0A059FJU2_9PROT Tryptophanase/L-cysteine desulfhydrase PLP-dependent OS=Hyphomonas jannaschiana VP2 OX=1280952 GN=tnaA PE=3 SV=1
MM1 pKa = 7.87KK2 pKa = 10.42SRR4 pKa = 11.84LLASSVFAGAAFMAQASMLAVAQEE28 pKa = 3.78ADD30 pKa = 3.61EE31 pKa = 5.21AVDD34 pKa = 3.98TVATTTEE41 pKa = 4.14DD42 pKa = 2.82ATARR46 pKa = 11.84QEE48 pKa = 4.25TVYY51 pKa = 9.58VTGSRR56 pKa = 11.84IAQPNMTTTSPVTSVSANDD75 pKa = 3.36VKK77 pKa = 11.11LQGVTRR83 pKa = 11.84VEE85 pKa = 4.23DD86 pKa = 4.08LVTQLPQAFAAQNSTVSNGASGTATVSLRR115 pKa = 11.84NLDD118 pKa = 3.81LGGSATRR125 pKa = 11.84TLVLVDD131 pKa = 4.2GKK133 pKa = 10.23RR134 pKa = 11.84LPYY137 pKa = 10.38GSPNDD142 pKa = 3.65AAADD146 pKa = 3.88LNLIPGQMVEE156 pKa = 4.32RR157 pKa = 11.84VDD159 pKa = 3.84VLTGGASAVYY169 pKa = 10.16GSDD172 pKa = 3.67AIAGVVNFIMKK183 pKa = 10.29DD184 pKa = 3.28DD185 pKa = 4.09FEE187 pKa = 4.75GVQIDD192 pKa = 3.96TQYY195 pKa = 11.88GFYY198 pKa = 10.19QHH200 pKa = 6.61NNDD203 pKa = 3.43YY204 pKa = 8.78DD205 pKa = 4.13TNGDD209 pKa = 3.67LRR211 pKa = 11.84DD212 pKa = 3.99VIAQRR217 pKa = 11.84AVGNPSQFALPDD229 pKa = 4.04DD230 pKa = 4.17NVIDD234 pKa = 4.89GYY236 pKa = 10.9SKK238 pKa = 10.84EE239 pKa = 3.69ITAIMGVNTDD249 pKa = 4.01DD250 pKa = 4.37GRR252 pKa = 11.84GNLTAWFGYY261 pKa = 9.85RR262 pKa = 11.84NNDD265 pKa = 2.84EE266 pKa = 4.38VLQGDD271 pKa = 4.18RR272 pKa = 11.84DD273 pKa = 3.87YY274 pKa = 11.25SACAIGSATATGFTCGGSSTSFPGRR299 pKa = 11.84FTDD302 pKa = 5.61FDD304 pKa = 3.99AFNYY308 pKa = 9.8TIDD311 pKa = 3.79PATGEE316 pKa = 4.08FRR318 pKa = 11.84DD319 pKa = 4.16FDD321 pKa = 4.22AATDD325 pKa = 3.65QYY327 pKa = 11.78NYY329 pKa = 10.94GPLNFYY335 pKa = 10.2QRR337 pKa = 11.84PDD339 pKa = 3.08EE340 pKa = 4.62RR341 pKa = 11.84YY342 pKa = 6.83TAGFNGHH349 pKa = 5.44YY350 pKa = 9.98QINDD354 pKa = 3.14YY355 pKa = 11.44AEE357 pKa = 5.38AYY359 pKa = 6.49TQFMFMDD366 pKa = 4.12YY367 pKa = 10.78QSNAQIAPSGNFFATTSINCDD388 pKa = 3.12NPLISASQLADD399 pKa = 3.24IGCTSANIAAGDD411 pKa = 4.13SIGMYY416 pKa = 8.89IARR419 pKa = 11.84RR420 pKa = 11.84NVEE423 pKa = 3.56GGGRR427 pKa = 11.84QDD429 pKa = 3.18HH430 pKa = 6.83LGYY433 pKa = 8.35QTYY436 pKa = 10.45RR437 pKa = 11.84GVAGLRR443 pKa = 11.84GDD445 pKa = 4.48LYY447 pKa = 11.05KK448 pKa = 11.06APGWSYY454 pKa = 11.17DD455 pKa = 2.93ISASFAQVTLSRR467 pKa = 11.84SYY469 pKa = 11.12RR470 pKa = 11.84NEE472 pKa = 3.49FSITRR477 pKa = 11.84LNRR480 pKa = 11.84ALNVVDD486 pKa = 5.59DD487 pKa = 4.38GTGTAVCASVLDD499 pKa = 4.26GTDD502 pKa = 3.78PNCVPWDD509 pKa = 3.27IFTVGNVTPEE519 pKa = 3.68ALAYY523 pKa = 10.3LQVPLLQTGTTEE535 pKa = 3.88QNIVSGVLTGDD546 pKa = 3.79LGQYY550 pKa = 9.63GFQLPSANSGLQVAIGAEE568 pKa = 3.98YY569 pKa = 10.47RR570 pKa = 11.84RR571 pKa = 11.84DD572 pKa = 3.67ALEE575 pKa = 4.78SITDD579 pKa = 3.5NSFATGDD586 pKa = 3.66GAGQGGPTIGLSGAVDD602 pKa = 3.89SYY604 pKa = 12.28DD605 pKa = 3.33MFAEE609 pKa = 4.3FQMPLVEE616 pKa = 4.48GKK618 pKa = 9.74PGVEE622 pKa = 3.89LLSIEE627 pKa = 4.03GAYY630 pKa = 9.94RR631 pKa = 11.84YY632 pKa = 10.02SDD634 pKa = 3.71YY635 pKa = 10.94STGISADD642 pKa = 3.43SYY644 pKa = 11.55KK645 pKa = 10.48IGGDD649 pKa = 3.61YY650 pKa = 11.13APTSDD655 pKa = 2.57IRR657 pKa = 11.84FRR659 pKa = 11.84ASFQKK664 pKa = 10.53AVRR667 pKa = 11.84APNVIDD673 pKa = 4.84LFQAQGFNLFDD684 pKa = 6.12LDD686 pKa = 6.14DD687 pKa = 4.94DD688 pKa = 4.9LCDD691 pKa = 3.95FTDD694 pKa = 3.93PAGDD698 pKa = 3.8GTGGAACIGTNPWQVTQAQADD719 pKa = 4.03GGALGSPAGQYY730 pKa = 10.94NYY732 pKa = 10.71LQGGNPDD739 pKa = 3.89LQPEE743 pKa = 4.16EE744 pKa = 5.61AEE746 pKa = 4.09TLTIGFVATPTFLPGFSASVDD767 pKa = 3.67YY768 pKa = 11.36YY769 pKa = 11.49NIDD772 pKa = 3.11ITDD775 pKa = 5.07AISTVGSSVTMQLCYY790 pKa = 10.58LQGDD794 pKa = 3.74ADD796 pKa = 3.77ACQRR800 pKa = 11.84IQRR803 pKa = 11.84NANGQLWVGTGNVVDD818 pKa = 4.84LNVNIGGVEE827 pKa = 3.85TSGFDD832 pKa = 2.89ISAAYY837 pKa = 9.45GFDD840 pKa = 3.07MGKK843 pKa = 9.59YY844 pKa = 9.96GSMNLSMNGTLLDD857 pKa = 4.14SFDD860 pKa = 3.76VDD862 pKa = 3.8PVGNAAYY869 pKa = 9.8AYY871 pKa = 10.02DD872 pKa = 3.89CVGKK876 pKa = 10.77YY877 pKa = 10.94GNDD880 pKa = 3.47CFTPTPEE887 pKa = 3.44WRR889 pKa = 11.84HH890 pKa = 5.2RR891 pKa = 11.84ARR893 pKa = 11.84ASWVTPVDD901 pKa = 3.74GLDD904 pKa = 4.66LSATWRR910 pKa = 11.84YY911 pKa = 9.52IGSADD916 pKa = 4.69LDD918 pKa = 3.67TGVTNRR924 pKa = 11.84VDD926 pKa = 3.52STLEE930 pKa = 3.65EE931 pKa = 3.81QNYY934 pKa = 9.8IDD936 pKa = 6.41LAGQWAAKK944 pKa = 9.84DD945 pKa = 3.36WVSFRR950 pKa = 11.84FGVNNVLDD958 pKa = 4.89DD959 pKa = 5.01DD960 pKa = 5.08PPLSASTGTTGNGNTYY976 pKa = 8.89PQTYY980 pKa = 9.88DD981 pKa = 2.69AMGRR985 pKa = 11.84YY986 pKa = 8.69FFVGATFDD994 pKa = 3.57FF995 pKa = 4.93

Molecular weight: 106.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A059FKR9|A0A059FKR9_9PROT Penicillin-binding protein 2 OS=Hyphomonas jannaschiana VP2 OX=1280952 GN=HJA_00830 PE=4 SV=1
MM1 pKa = 7.44SKK3 pKa = 10.4SPNSVLHH10 pKa = 5.94PAKK13 pKa = 10.23KK14 pKa = 9.76PRR16 pKa = 11.84VKK18 pKa = 10.33RR19 pKa = 11.84RR20 pKa = 11.84NATWKK25 pKa = 10.6ANLGKK30 pKa = 9.88RR31 pKa = 11.84AKK33 pKa = 10.47VRR35 pKa = 11.84VFGNTKK41 pKa = 9.19RR42 pKa = 11.84ARR44 pKa = 11.84IARR47 pKa = 11.84KK48 pKa = 9.21KK49 pKa = 10.51AAGKK53 pKa = 10.03

Molecular weight: 5.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3505

0

3505

1100037

19

2026

313.8

34.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.059 ± 0.056

0.843 ± 0.012

6.206 ± 0.039

6.35 ± 0.039

3.826 ± 0.026

8.595 ± 0.041

1.908 ± 0.019

5.091 ± 0.031

3.634 ± 0.039

9.673 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.659 ± 0.021

2.712 ± 0.022

5.191 ± 0.031

3.08 ± 0.019

6.407 ± 0.041

5.534 ± 0.03

5.4 ± 0.031

6.999 ± 0.031

1.419 ± 0.018

2.416 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski