Amino acid dipepetide frequency for Synechococcus phage S-CAM4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.46AlaAla: 5.46 ± 0.479
0.592AlaCys: 0.592 ± 0.093
3.996AlaAsp: 3.996 ± 0.243
3.552AlaGlu: 3.552 ± 0.321
2.532AlaPhe: 2.532 ± 0.216
6.15AlaGly: 6.15 ± 0.544
0.839AlaHis: 0.839 ± 0.128
3.815AlaIle: 3.815 ± 0.278
3.552AlaLys: 3.552 ± 0.314
4.341AlaLeu: 4.341 ± 0.368
1.332AlaMet: 1.332 ± 0.165
4.572AlaAsn: 4.572 ± 0.372
2.286AlaPro: 2.286 ± 0.204
2.154AlaGln: 2.154 ± 0.203
2.384AlaArg: 2.384 ± 0.216
4.621AlaSer: 4.621 ± 0.338
5.64AlaThr: 5.64 ± 0.354
4.111AlaVal: 4.111 ± 0.239
0.526AlaTrp: 0.526 ± 0.08
2.269AlaTyr: 2.269 ± 0.176
0.0AlaXaa: 0.0 ± 0.0
Cys
0.543CysAla: 0.543 ± 0.087
0.082CysCys: 0.082 ± 0.04
0.658CysAsp: 0.658 ± 0.082
0.51CysGlu: 0.51 ± 0.087
0.345CysPhe: 0.345 ± 0.084
0.658CysGly: 0.658 ± 0.125
0.181CysHis: 0.181 ± 0.059
0.411CysIle: 0.411 ± 0.086
0.543CysLys: 0.543 ± 0.101
0.592CysLeu: 0.592 ± 0.116
0.148CysMet: 0.148 ± 0.06
0.362CysAsn: 0.362 ± 0.07
0.329CysPro: 0.329 ± 0.089
0.411CysGln: 0.411 ± 0.089
0.428CysArg: 0.428 ± 0.096
0.526CysSer: 0.526 ± 0.105
0.526CysThr: 0.526 ± 0.096
0.559CysVal: 0.559 ± 0.11
0.164CysTrp: 0.164 ± 0.058
0.312CysTyr: 0.312 ± 0.07
0.0CysXaa: 0.0 ± 0.0
Asp
4.259AspAla: 4.259 ± 0.334
0.526AspCys: 0.526 ± 0.101
4.308AspAsp: 4.308 ± 0.277
3.667AspGlu: 3.667 ± 0.334
2.976AspPhe: 2.976 ± 0.193
5.624AspGly: 5.624 ± 0.297
1.052AspHis: 1.052 ± 0.138
4.473AspIle: 4.473 ± 0.293
3.503AspLys: 3.503 ± 0.315
4.785AspLeu: 4.785 ± 0.273
1.431AspMet: 1.431 ± 0.2
4.292AspAsn: 4.292 ± 0.253
2.944AspPro: 2.944 ± 0.256
2.006AspGln: 2.006 ± 0.187
2.5AspArg: 2.5 ± 0.218
4.539AspSer: 4.539 ± 0.28
4.522AspThr: 4.522 ± 0.286
4.506AspVal: 4.506 ± 0.251
1.036AspTrp: 1.036 ± 0.143
2.993AspTyr: 2.993 ± 0.253
0.0AspXaa: 0.0 ± 0.0
Glu
2.894GluAla: 2.894 ± 0.277
0.559GluCys: 0.559 ± 0.107
3.766GluAsp: 3.766 ± 0.268
4.489GluGlu: 4.489 ± 0.467
3.108GluPhe: 3.108 ± 0.234
3.7GluGly: 3.7 ± 0.226
1.02GluHis: 1.02 ± 0.161
4.243GluIle: 4.243 ± 0.311
3.684GluLys: 3.684 ± 0.35
4.868GluLeu: 4.868 ± 0.406
1.661GluMet: 1.661 ± 0.236
2.976GluAsn: 2.976 ± 0.232
1.677GluPro: 1.677 ± 0.194
2.154GluGln: 2.154 ± 0.226
2.286GluArg: 2.286 ± 0.236
3.815GluSer: 3.815 ± 0.34
3.782GluThr: 3.782 ± 0.29
4.456GluVal: 4.456 ± 0.246
1.052GluTrp: 1.052 ± 0.154
2.648GluTyr: 2.648 ± 0.224
0.0GluXaa: 0.0 ± 0.0
Phe
2.73PheAla: 2.73 ± 0.253
0.543PheCys: 0.543 ± 0.104
3.404PheAsp: 3.404 ± 0.235
2.45PheGlu: 2.45 ± 0.243
1.727PhePhe: 1.727 ± 0.196
2.713PheGly: 2.713 ± 0.257
0.707PheHis: 0.707 ± 0.125
2.549PheIle: 2.549 ± 0.232
2.286PheLys: 2.286 ± 0.188
3.174PheLeu: 3.174 ± 0.223
0.806PheMet: 0.806 ± 0.142
2.944PheAsn: 2.944 ± 0.191
1.529PhePro: 1.529 ± 0.169
1.496PheGln: 1.496 ± 0.154
1.677PheArg: 1.677 ± 0.192
3.47PheSer: 3.47 ± 0.236
3.897PheThr: 3.897 ± 0.301
2.878PheVal: 2.878 ± 0.214
0.428PheTrp: 0.428 ± 0.082
1.973PheTyr: 1.973 ± 0.173
0.0PheXaa: 0.0 ± 0.0
Gly
5.41GlyAla: 5.41 ± 0.519
0.51GlyCys: 0.51 ± 0.088
5.065GlyAsp: 5.065 ± 0.444
4.012GlyGlu: 4.012 ± 0.231
3.075GlyPhe: 3.075 ± 0.231
8.485GlyGly: 8.485 ± 0.948
1.052GlyHis: 1.052 ± 0.143
4.128GlyIle: 4.128 ± 0.267
3.503GlyLys: 3.503 ± 0.266
4.572GlyLeu: 4.572 ± 0.302
1.299GlyMet: 1.299 ± 0.203
5.492GlyAsn: 5.492 ± 0.417
1.842GlyPro: 1.842 ± 0.161
2.993GlyGln: 2.993 ± 0.219
2.845GlyArg: 2.845 ± 0.19
7.745GlySer: 7.745 ± 0.555
7.038GlyThr: 7.038 ± 0.614
5.739GlyVal: 5.739 ± 0.383
0.789GlyTrp: 0.789 ± 0.136
3.124GlyTyr: 3.124 ± 0.242
0.0GlyXaa: 0.0 ± 0.0
His
0.724HisAla: 0.724 ± 0.119
0.099HisCys: 0.099 ± 0.041
1.085HisAsp: 1.085 ± 0.156
1.003HisGlu: 1.003 ± 0.156
0.608HisPhe: 0.608 ± 0.102
0.97HisGly: 0.97 ± 0.158
0.263HisHis: 0.263 ± 0.073
0.904HisIle: 0.904 ± 0.134
1.102HisLys: 1.102 ± 0.149
1.003HisLeu: 1.003 ± 0.16
0.329HisMet: 0.329 ± 0.083
0.74HisAsn: 0.74 ± 0.125
0.872HisPro: 0.872 ± 0.116
0.51HisGln: 0.51 ± 0.104
0.444HisArg: 0.444 ± 0.088
0.839HisSer: 0.839 ± 0.114
1.003HisThr: 1.003 ± 0.123
0.592HisVal: 0.592 ± 0.127
0.181HisTrp: 0.181 ± 0.055
0.872HisTyr: 0.872 ± 0.115
0.0HisXaa: 0.0 ± 0.0
Ile
4.193IleAla: 4.193 ± 0.279
0.608IleCys: 0.608 ± 0.113
4.785IleAsp: 4.785 ± 0.291
4.259IleGlu: 4.259 ± 0.274
2.467IlePhe: 2.467 ± 0.186
4.358IleGly: 4.358 ± 0.253
0.724IleHis: 0.724 ± 0.119
4.21IleIle: 4.21 ± 0.28
3.832IleLys: 3.832 ± 0.324
4.572IleLeu: 4.572 ± 0.321
0.937IleMet: 0.937 ± 0.143
4.374IleAsn: 4.374 ± 0.388
2.779IlePro: 2.779 ± 0.226
2.302IleGln: 2.302 ± 0.191
2.483IleArg: 2.483 ± 0.185
5.328IleSer: 5.328 ± 0.337
6.216IleThr: 6.216 ± 0.464
4.21IleVal: 4.21 ± 0.404
0.658IleTrp: 0.658 ± 0.105
1.973IleTyr: 1.973 ± 0.177
0.0IleXaa: 0.0 ± 0.0
Lys
2.565LysAla: 2.565 ± 0.267
0.493LysCys: 0.493 ± 0.08
3.305LysAsp: 3.305 ± 0.28
3.782LysGlu: 3.782 ± 0.361
2.483LysPhe: 2.483 ± 0.247
2.927LysGly: 2.927 ± 0.213
0.921LysHis: 0.921 ± 0.155
4.095LysIle: 4.095 ± 0.348
4.078LysLys: 4.078 ± 0.465
4.473LysLeu: 4.473 ± 0.307
1.513LysMet: 1.513 ± 0.225
2.861LysAsn: 2.861 ± 0.261
2.056LysPro: 2.056 ± 0.209
2.269LysGln: 2.269 ± 0.224
2.434LysArg: 2.434 ± 0.256
3.108LysSer: 3.108 ± 0.261
3.947LysThr: 3.947 ± 0.264
3.42LysVal: 3.42 ± 0.273
0.674LysTrp: 0.674 ± 0.131
2.96LysTyr: 2.96 ± 0.265
0.0LysXaa: 0.0 ± 0.0
Leu
4.604LeuAla: 4.604 ± 0.298
0.641LeuCys: 0.641 ± 0.099
5.246LeuAsp: 5.246 ± 0.283
4.917LeuGlu: 4.917 ± 0.339
2.648LeuPhe: 2.648 ± 0.206
4.736LeuGly: 4.736 ± 0.348
1.069LeuHis: 1.069 ± 0.146
4.16LeuIle: 4.16 ± 0.313
4.325LeuLys: 4.325 ± 0.406
5.229LeuLeu: 5.229 ± 0.361
1.496LeuMet: 1.496 ± 0.147
5.048LeuAsn: 5.048 ± 0.261
3.371LeuPro: 3.371 ± 0.197
2.746LeuGln: 2.746 ± 0.236
2.976LeuArg: 2.976 ± 0.214
5.361LeuSer: 5.361 ± 0.384
5.723LeuThr: 5.723 ± 0.309
4.276LeuVal: 4.276 ± 0.266
0.724LeuTrp: 0.724 ± 0.103
3.108LeuTyr: 3.108 ± 0.24
0.0LeuXaa: 0.0 ± 0.0
Met
1.414MetAla: 1.414 ± 0.197
0.197MetCys: 0.197 ± 0.073
1.168MetAsp: 1.168 ± 0.182
0.904MetGlu: 0.904 ± 0.159
0.707MetPhe: 0.707 ± 0.143
1.118MetGly: 1.118 ± 0.184
0.23MetHis: 0.23 ± 0.08
1.085MetIle: 1.085 ± 0.139
1.513MetLys: 1.513 ± 0.188
1.891MetLeu: 1.891 ± 0.251
0.724MetMet: 0.724 ± 0.133
1.283MetAsn: 1.283 ± 0.162
1.085MetPro: 1.085 ± 0.127
0.608MetGln: 0.608 ± 0.115
0.888MetArg: 0.888 ± 0.123
1.48MetSer: 1.48 ± 0.191
1.579MetThr: 1.579 ± 0.189
0.954MetVal: 0.954 ± 0.124
0.345MetTrp: 0.345 ± 0.08
0.74MetTyr: 0.74 ± 0.116
0.0MetXaa: 0.0 ± 0.0
Asn
3.799AsnAla: 3.799 ± 0.351
0.576AsnCys: 0.576 ± 0.104
3.963AsnAsp: 3.963 ± 0.258
3.42AsnGlu: 3.42 ± 0.325
3.305AsnPhe: 3.305 ± 0.234
5.048AsnGly: 5.048 ± 0.421
0.904AsnHis: 0.904 ± 0.135
4.555AsnIle: 4.555 ± 0.293
2.845AsnLys: 2.845 ± 0.223
4.983AsnLeu: 4.983 ± 0.324
1.036AsnMet: 1.036 ± 0.149
3.947AsnAsn: 3.947 ± 0.292
3.19AsnPro: 3.19 ± 0.228
2.5AsnGln: 2.5 ± 0.213
2.384AsnArg: 2.384 ± 0.188
4.276AsnSer: 4.276 ± 0.281
5.098AsnThr: 5.098 ± 0.443
4.226AsnVal: 4.226 ± 0.278
0.839AsnTrp: 0.839 ± 0.113
3.042AsnTyr: 3.042 ± 0.288
0.0AsnXaa: 0.0 ± 0.0
Pro
2.549ProAla: 2.549 ± 0.182
0.148ProCys: 0.148 ± 0.062
2.648ProAsp: 2.648 ± 0.21
2.582ProGlu: 2.582 ± 0.23
1.776ProPhe: 1.776 ± 0.136
3.256ProGly: 3.256 ± 0.297
0.543ProHis: 0.543 ± 0.104
2.302ProIle: 2.302 ± 0.241
1.842ProLys: 1.842 ± 0.2
2.631ProLeu: 2.631 ± 0.21
0.674ProMet: 0.674 ± 0.136
2.582ProAsn: 2.582 ± 0.188
1.776ProPro: 1.776 ± 0.204
1.25ProGln: 1.25 ± 0.149
1.316ProArg: 1.316 ± 0.137
3.388ProSer: 3.388 ± 0.231
3.42ProThr: 3.42 ± 0.249
2.68ProVal: 2.68 ± 0.189
0.411ProTrp: 0.411 ± 0.085
1.727ProTyr: 1.727 ± 0.171
0.0ProXaa: 0.0 ± 0.0
Gln
2.22GlnAla: 2.22 ± 0.198
0.28GlnCys: 0.28 ± 0.065
1.677GlnAsp: 1.677 ± 0.195
2.352GlnGlu: 2.352 ± 0.271
1.628GlnPhe: 1.628 ± 0.158
2.121GlnGly: 2.121 ± 0.164
0.74GlnHis: 0.74 ± 0.109
2.697GlnIle: 2.697 ± 0.191
2.056GlnLys: 2.056 ± 0.216
2.746GlnLeu: 2.746 ± 0.204
0.904GlnMet: 0.904 ± 0.133
2.121GlnAsn: 2.121 ± 0.192
1.217GlnPro: 1.217 ± 0.137
1.529GlnGln: 1.529 ± 0.16
1.546GlnArg: 1.546 ± 0.137
2.532GlnSer: 2.532 ± 0.226
2.467GlnThr: 2.467 ± 0.228
2.615GlnVal: 2.615 ± 0.239
0.608GlnTrp: 0.608 ± 0.094
1.825GlnTyr: 1.825 ± 0.174
0.0GlnXaa: 0.0 ± 0.0
Arg
2.549ArgAla: 2.549 ± 0.206
0.329ArgCys: 0.329 ± 0.081
2.532ArgAsp: 2.532 ± 0.176
2.121ArgGlu: 2.121 ± 0.196
1.71ArgPhe: 1.71 ± 0.173
2.96ArgGly: 2.96 ± 0.215
0.51ArgHis: 0.51 ± 0.112
2.664ArgIle: 2.664 ± 0.201
2.417ArgLys: 2.417 ± 0.227
3.272ArgLeu: 3.272 ± 0.211
0.921ArgMet: 0.921 ± 0.13
1.99ArgAsn: 1.99 ± 0.222
1.348ArgPro: 1.348 ± 0.137
1.447ArgGln: 1.447 ± 0.139
1.99ArgArg: 1.99 ± 0.21
2.204ArgSer: 2.204 ± 0.171
2.154ArgThr: 2.154 ± 0.194
2.763ArgVal: 2.763 ± 0.225
0.395ArgTrp: 0.395 ± 0.067
2.088ArgTyr: 2.088 ± 0.193
0.0ArgXaa: 0.0 ± 0.0
Ser
5.41SerAla: 5.41 ± 0.353
0.312SerCys: 0.312 ± 0.082
3.947SerAsp: 3.947 ± 0.246
3.355SerGlu: 3.355 ± 0.251
2.976SerPhe: 2.976 ± 0.279
8.748SerGly: 8.748 ± 0.652
0.921SerHis: 0.921 ± 0.128
5.723SerIle: 5.723 ± 0.38
3.634SerLys: 3.634 ± 0.332
5.032SerLeu: 5.032 ± 0.303
1.365SerMet: 1.365 ± 0.207
5.246SerAsn: 5.246 ± 0.303
2.845SerPro: 2.845 ± 0.274
2.532SerGln: 2.532 ± 0.211
2.302SerArg: 2.302 ± 0.176
6.907SerSer: 6.907 ± 0.481
5.591SerThr: 5.591 ± 0.35
5.229SerVal: 5.229 ± 0.347
0.806SerTrp: 0.806 ± 0.133
2.845SerTyr: 2.845 ± 0.209
0.0SerXaa: 0.0 ± 0.0
Thr
5.575ThrAla: 5.575 ± 0.386
0.625ThrCys: 0.625 ± 0.114
4.621ThrAsp: 4.621 ± 0.256
3.832ThrGlu: 3.832 ± 0.23
3.864ThrPhe: 3.864 ± 0.252
6.972ThrGly: 6.972 ± 0.579
0.855ThrHis: 0.855 ± 0.131
5.805ThrIle: 5.805 ± 0.497
3.026ThrLys: 3.026 ± 0.29
6.2ThrLeu: 6.2 ± 0.39
1.003ThrMet: 1.003 ± 0.133
4.851ThrAsn: 4.851 ± 0.358
3.881ThrPro: 3.881 ± 0.314
2.73ThrGln: 2.73 ± 0.186
2.187ThrArg: 2.187 ± 0.182
6.052ThrSer: 6.052 ± 0.445
6.348ThrThr: 6.348 ± 0.474
5.821ThrVal: 5.821 ± 0.421
0.707ThrTrp: 0.707 ± 0.113
3.404ThrTyr: 3.404 ± 0.27
0.0ThrXaa: 0.0 ± 0.0
Val
4.374ValAla: 4.374 ± 0.28
0.526ValCys: 0.526 ± 0.106
5.591ValAsp: 5.591 ± 0.367
4.374ValGlu: 4.374 ± 0.308
3.059ValPhe: 3.059 ± 0.217
4.95ValGly: 4.95 ± 0.329
0.674ValHis: 0.674 ± 0.12
3.98ValIle: 3.98 ± 0.307
3.453ValLys: 3.453 ± 0.255
4.374ValLeu: 4.374 ± 0.27
1.233ValMet: 1.233 ± 0.155
4.21ValAsn: 4.21 ± 0.272
2.565ValPro: 2.565 ± 0.218
2.138ValGln: 2.138 ± 0.155
2.96ValArg: 2.96 ± 0.212
5.986ValSer: 5.986 ± 0.421
5.525ValThr: 5.525 ± 0.44
4.802ValVal: 4.802 ± 0.422
0.543ValTrp: 0.543 ± 0.088
2.5ValTyr: 2.5 ± 0.235
0.0ValXaa: 0.0 ± 0.0
Trp
0.74TrpAla: 0.74 ± 0.11
0.132TrpCys: 0.132 ± 0.043
1.069TrpAsp: 1.069 ± 0.125
0.724TrpGlu: 0.724 ± 0.118
0.329TrpPhe: 0.329 ± 0.091
0.789TrpGly: 0.789 ± 0.13
0.214TrpHis: 0.214 ± 0.062
0.773TrpIle: 0.773 ± 0.125
0.691TrpLys: 0.691 ± 0.115
0.51TrpLeu: 0.51 ± 0.102
0.329TrpMet: 0.329 ± 0.085
0.855TrpAsn: 0.855 ± 0.117
0.164TrpPro: 0.164 ± 0.062
0.395TrpGln: 0.395 ± 0.088
0.658TrpArg: 0.658 ± 0.117
0.855TrpSer: 0.855 ± 0.137
0.773TrpThr: 0.773 ± 0.123
0.773TrpVal: 0.773 ± 0.12
0.164TrpTrp: 0.164 ± 0.056
0.559TrpTyr: 0.559 ± 0.093
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.713TyrAla: 2.713 ± 0.238
0.526TyrCys: 0.526 ± 0.106
3.108TyrAsp: 3.108 ± 0.219
2.532TyrGlu: 2.532 ± 0.224
2.056TyrPhe: 2.056 ± 0.197
2.434TyrGly: 2.434 ± 0.197
0.724TyrHis: 0.724 ± 0.121
2.697TyrIle: 2.697 ± 0.185
2.483TyrLys: 2.483 ± 0.193
3.157TyrLeu: 3.157 ± 0.207
0.756TyrMet: 0.756 ± 0.12
3.19TyrAsn: 3.19 ± 0.198
1.743TyrPro: 1.743 ± 0.165
1.71TyrGln: 1.71 ± 0.185
1.677TyrArg: 1.677 ± 0.173
2.697TyrSer: 2.697 ± 0.175
3.108TyrThr: 3.108 ± 0.261
3.207TyrVal: 3.207 ± 0.215
0.444TyrTrp: 0.444 ± 0.082
1.973TyrTyr: 1.973 ± 0.242
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 238 proteins (60812 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski