Symbiodinium microadriaticum (Dinoflagellate) (Zooxanthella microadriatica)

Taxonomy: cellular organisms; Eukaryota; Sar; Alveolata; Dinophyceae; Suessiales; Symbiodiniaceae; Symbiodinium

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 43269 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q9EWL1|A0A1Q9EWL1_SYMMI Pepsin A OS=Symbiodinium microadriaticum OX=2951 GN=PGA PE=3 SV=1
MM1 pKa = 7.92DD2 pKa = 4.93CEE4 pKa = 3.63KK5 pKa = 10.61CAAYY9 pKa = 10.23RR10 pKa = 11.84EE11 pKa = 4.5SLPPEE16 pKa = 3.94HH17 pKa = 6.63RR18 pKa = 11.84VYY20 pKa = 10.0WPCGYY25 pKa = 10.33CPTGRR30 pKa = 11.84GGLCEE35 pKa = 3.91RR36 pKa = 11.84RR37 pKa = 11.84VFCSVANDD45 pKa = 3.95GVSEE49 pKa = 4.59DD50 pKa = 3.51GGTVKK55 pKa = 10.02MAMNMVVVSMASQDD69 pKa = 3.8DD70 pKa = 3.92SDD72 pKa = 4.0VADD75 pKa = 3.55VHH77 pKa = 7.0GSADD81 pKa = 4.05DD82 pKa = 4.73DD83 pKa = 4.55GSAADD88 pKa = 5.13DD89 pKa = 4.04DD90 pKa = 4.75NKK92 pKa = 11.21YY93 pKa = 11.17GDD95 pKa = 5.27DD96 pKa = 5.59DD97 pKa = 6.45DD98 pKa = 7.49DD99 pKa = 7.24GDD101 pKa = 4.37DD102 pKa = 3.48

Molecular weight: 10.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q9BQX4|A0A1Q9BQX4_SYMMI Uncharacterized protein (Fragment) OS=Symbiodinium microadriaticum OX=2951 GN=AK812_SmicGene47955 PE=4 SV=1
KKK2 pKa = 10.0ATLSTQALRR11 pKa = 11.84VRR13 pKa = 11.84MLRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84SPTTIGKKK26 pKa = 9.51SSRR29 pKa = 11.84ASP

Molecular weight: 3.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

43269

0

43269

32314097

29

18700

746.8

81.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.286 ± 0.015

2.038 ± 0.007

5.392 ± 0.012

6.697 ± 0.021

3.437 ± 0.008

7.088 ± 0.014

2.374 ± 0.006

3.352 ± 0.009

4.41 ± 0.013

10.033 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.253 ± 0.006

2.414 ± 0.009

5.567 ± 0.011

4.626 ± 0.03

6.661 ± 0.011

7.328 ± 0.011

4.825 ± 0.009

6.927 ± 0.022

1.809 ± 0.005

1.996 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski