Eubacterium pyruvativorans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; Eubacterium

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1994 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I7IMQ8|A0A1I7IMQ8_9FIRM Uncharacterized protein OS=Eubacterium pyruvativorans OX=155865 GN=SAMN05216508_1573 PE=4 SV=1
MM1 pKa = 7.81DD2 pKa = 5.72LEE4 pKa = 4.13NQKK7 pKa = 10.06RR8 pKa = 11.84VKK10 pKa = 10.63DD11 pKa = 3.74FAEE14 pKa = 4.07QYY16 pKa = 9.92GPEE19 pKa = 4.07NVVVVLGAAEE29 pKa = 4.39GEE31 pKa = 4.39AAGLAAEE38 pKa = 4.6TVTNGDD44 pKa = 3.31PTYY47 pKa = 10.7AGPLTEE53 pKa = 4.28VQLGLTVYY61 pKa = 10.36HH62 pKa = 5.73VVEE65 pKa = 4.46PEE67 pKa = 3.85MKK69 pKa = 10.44EE70 pKa = 4.08EE71 pKa = 4.16FDD73 pKa = 3.67PDD75 pKa = 3.77VYY77 pKa = 11.3DD78 pKa = 3.84EE79 pKa = 4.57QISMMEE85 pKa = 4.02MVLDD89 pKa = 3.56VDD91 pKa = 5.69DD92 pKa = 6.29IIDD95 pKa = 3.77EE96 pKa = 4.18MSDD99 pKa = 4.26IRR101 pKa = 11.84DD102 pKa = 3.8EE103 pKa = 4.3YY104 pKa = 11.21CKK106 pKa = 10.89YY107 pKa = 10.86LL108 pKa = 3.8

Molecular weight: 12.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I7G8Q2|A0A1I7G8Q2_9FIRM Transcriptional regulator MarR family OS=Eubacterium pyruvativorans OX=155865 GN=SAMN05216508_10566 PE=4 SV=1
MM1 pKa = 7.39NNEE4 pKa = 3.96KK5 pKa = 10.17NQKK8 pKa = 10.03KK9 pKa = 10.25NEE11 pKa = 4.02TSSRR15 pKa = 11.84AGGTLRR21 pKa = 11.84PPMDD25 pKa = 3.41VRR27 pKa = 11.84SGVLWTLVLGILLPALGAFLLKK49 pKa = 10.45NRR51 pKa = 11.84SDD53 pKa = 4.03LYY55 pKa = 10.25VTLAADD61 pKa = 3.65GAGAVIAFLLLRR73 pKa = 11.84GRR75 pKa = 11.84ARR77 pKa = 11.84AGGTVSRR84 pKa = 11.84RR85 pKa = 11.84FTAKK89 pKa = 10.58DD90 pKa = 3.63LILSVLLMFFTLHH103 pKa = 6.57CLNGVCRR110 pKa = 11.84IFLPEE115 pKa = 3.7QTASSGAAARR125 pKa = 11.84MAGSGTIGAGTGASGEE141 pKa = 4.05ILLAVIGLAVLPAIYY156 pKa = 10.19EE157 pKa = 4.1EE158 pKa = 4.26LLMRR162 pKa = 11.84GILFRR167 pKa = 11.84SLRR170 pKa = 11.84TAVGFWPAALLSAAFWGIWHH190 pKa = 7.3WNPAQCVTAFGAGLVLALLMEE211 pKa = 4.94VYY213 pKa = 10.6DD214 pKa = 4.15SLALVILLHH223 pKa = 5.85MANNLMSVFGWLLARR238 pKa = 11.84WIPGVLRR245 pKa = 11.84GPGASAGGDD254 pKa = 3.79GILLLAEE261 pKa = 4.17GLAAVWLFRR270 pKa = 11.84KK271 pKa = 9.79VKK273 pKa = 9.51TGEE276 pKa = 3.76PLRR279 pKa = 11.84RR280 pKa = 11.84RR281 pKa = 3.72

Molecular weight: 29.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1994

0

1994

657711

33

1618

329.8

36.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.346 ± 0.064

1.388 ± 0.021

5.913 ± 0.042

7.443 ± 0.059

3.846 ± 0.036

7.859 ± 0.052

1.876 ± 0.024

6.479 ± 0.043

5.758 ± 0.053

8.689 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.197 ± 0.028

3.678 ± 0.034

3.85 ± 0.033

2.915 ± 0.026

6.27 ± 0.061

5.594 ± 0.037

5.474 ± 0.046

7.009 ± 0.042

0.87 ± 0.017

3.546 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski