Amino acid dipepetide frequency for Clostridium perfringens (strain 13 / Type A)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.311AlaAla: 3.311 ± 0.093	0.68AlaCys: 0.68 ± 0.03	2.301AlaAsp: 2.301 ± 0.058	3.381AlaGlu: 3.381 ± 0.076	2.615AlaPhe: 2.615 ± 0.055	3.696AlaGly: 3.696 ± 0.086	0.825AlaHis: 0.825 ± 0.03	5.53AlaIle: 5.53 ± 0.091	4.946AlaLys: 4.946 ± 0.093	6.099AlaLeu: 6.099 ± 0.11	1.663AlaMet: 1.663 ± 0.046	2.76AlaAsn: 2.76 ± 0.062	1.45AlaPro: 1.45 ± 0.045	1.256AlaGln: 1.256 ± 0.046	1.868AlaArg: 1.868 ± 0.052	3.305AlaSer: 3.305 ± 0.063	2.741AlaThr: 2.741 ± 0.067	3.449AlaVal: 3.449 ± 0.082	0.391AlaTrp: 0.391 ± 0.025	2.19AlaTyr: 2.19 ± 0.051	0.0AlaXaa: 0.0 ± 0.0
Cys	0.561CysAla: 0.561 ± 0.024	0.193CysCys: 0.193 ± 0.016	0.681CysAsp: 0.681 ± 0.03	0.805CysGlu: 0.805 ± 0.034	0.465CysPhe: 0.465 ± 0.023	1.124CysGly: 1.124 ± 0.045	0.201CysHis: 0.201 ± 0.016	1.025CysIle: 1.025 ± 0.04	0.988CysLys: 0.988 ± 0.039	0.868CysLeu: 0.868 ± 0.03	0.222CysMet: 0.222 ± 0.016	0.743CysAsn: 0.743 ± 0.029	0.419CysPro: 0.419 ± 0.023	0.193CysGln: 0.193 ± 0.016	0.389CysArg: 0.389 ± 0.024	0.672CysSer: 0.672 ± 0.031	0.564CysThr: 0.564 ± 0.027	0.624CysVal: 0.624 ± 0.028	0.05CysTrp: 0.05 ± 0.008	0.418CysTyr: 0.418 ± 0.022	0.0CysXaa: 0.0 ± 0.0
Asp	2.488AspAla: 2.488 ± 0.061	0.589AspCys: 0.589 ± 0.03	2.608AspAsp: 2.608 ± 0.058	4.885AspGlu: 4.885 ± 0.077	3.113AspPhe: 3.113 ± 0.063	3.529AspGly: 3.529 ± 0.067	0.625AspHis: 0.625 ± 0.028	5.627AspIle: 5.627 ± 0.1	5.367AspLys: 5.367 ± 0.082	5.607AspLeu: 5.607 ± 0.076	1.478AspMet: 1.478 ± 0.043	3.253AspAsn: 3.253 ± 0.06	1.334AspPro: 1.334 ± 0.042	0.854AspGln: 0.854 ± 0.033	1.775AspArg: 1.775 ± 0.047	3.308AspSer: 3.308 ± 0.062	2.267AspThr: 2.267 ± 0.063	3.545AspVal: 3.545 ± 0.064	0.376AspTrp: 0.376 ± 0.026	2.693AspTyr: 2.693 ± 0.061	0.0AspXaa: 0.0 ± 0.0
Glu	4.512GluAla: 4.512 ± 0.097	0.751GluCys: 0.751 ± 0.035	4.736GluAsp: 4.736 ± 0.069	8.657GluGlu: 8.657 ± 0.138	3.563GluPhe: 3.563 ± 0.075	5.084GluGly: 5.084 ± 0.088	0.897GluHis: 0.897 ± 0.034	7.469GluIle: 7.469 ± 0.096	8.586GluLys: 8.586 ± 0.112	7.242GluLeu: 7.242 ± 0.116	1.916GluMet: 1.916 ± 0.049	6.033GluAsn: 6.033 ± 0.096	1.448GluPro: 1.448 ± 0.038	1.455GluGln: 1.455 ± 0.048	2.826GluArg: 2.826 ± 0.065	3.968GluSer: 3.968 ± 0.073	2.96GluThr: 2.96 ± 0.065	5.731GluVal: 5.731 ± 0.103	0.449GluTrp: 0.449 ± 0.025	3.131GluTyr: 3.131 ± 0.065	0.0GluXaa: 0.0 ± 0.0
Phe	2.325PheAla: 2.325 ± 0.052	0.47PheCys: 0.47 ± 0.027	2.546PheAsp: 2.546 ± 0.054	3.005PheGlu: 3.005 ± 0.056	2.178PhePhe: 2.178 ± 0.061	3.009PheGly: 3.009 ± 0.072	0.548PheHis: 0.548 ± 0.028	4.78PheIle: 4.78 ± 0.094	4.465PheLys: 4.465 ± 0.081	4.461PheLeu: 4.461 ± 0.086	1.255PheMet: 1.255 ± 0.038	3.208PheAsn: 3.208 ± 0.069	1.135PhePro: 1.135 ± 0.04	0.865PheGln: 0.865 ± 0.03	1.354PheArg: 1.354 ± 0.042	3.152PheSer: 3.152 ± 0.07	2.361PheThr: 2.361 ± 0.053	2.712PheVal: 2.712 ± 0.064	0.326PheTrp: 0.326 ± 0.019	1.757PheTyr: 1.757 ± 0.051	0.0PheXaa: 0.0 ± 0.0
Gly	4.237GlyAla: 4.237 ± 0.09	0.879GlyCys: 0.879 ± 0.038	3.622GlyAsp: 3.622 ± 0.061	5.295GlyGlu: 5.295 ± 0.094	3.304GlyPhe: 3.304 ± 0.066	4.391GlyGly: 4.391 ± 0.104	0.98GlyHis: 0.98 ± 0.035	6.852GlyIle: 6.852 ± 0.11	5.657GlyLys: 5.657 ± 0.096	5.724GlyLeu: 5.724 ± 0.102	1.687GlyMet: 1.687 ± 0.05	3.564GlyAsn: 3.564 ± 0.074	1.281GlyPro: 1.281 ± 0.066	1.339GlyGln: 1.339 ± 0.039	2.264GlyArg: 2.264 ± 0.056	3.6GlySer: 3.6 ± 0.064	3.405GlyThr: 3.405 ± 0.069	5.124GlyVal: 5.124 ± 0.087	0.547GlyTrp: 0.547 ± 0.042	2.845GlyTyr: 2.845 ± 0.058	0.0GlyXaa: 0.0 ± 0.0
His	0.595HisAla: 0.595 ± 0.025	0.175HisCys: 0.175 ± 0.014	0.707HisAsp: 0.707 ± 0.028	0.875HisGlu: 0.875 ± 0.035	0.564HisPhe: 0.564 ± 0.027	1.039HisGly: 1.039 ± 0.032	0.278HisHis: 0.278 ± 0.019	1.242HisIle: 1.242 ± 0.039	1.083HisLys: 1.083 ± 0.034	1.106HisLeu: 1.106 ± 0.035	0.346HisMet: 0.346 ± 0.016	0.779HisAsn: 0.779 ± 0.03	0.555HisPro: 0.555 ± 0.028	0.278HisGln: 0.278 ± 0.018	0.542HisArg: 0.542 ± 0.028	0.8HisSer: 0.8 ± 0.027	0.588HisThr: 0.588 ± 0.026	0.752HisVal: 0.752 ± 0.031	0.096HisTrp: 0.096 ± 0.01	0.493HisTyr: 0.493 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	5.468IleAla: 5.468 ± 0.104	1.123IleCys: 1.123 ± 0.038	5.679IleAsp: 5.679 ± 0.09	7.224IleGlu: 7.224 ± 0.101	4.418IlePhe: 4.418 ± 0.098	6.07IleGly: 6.07 ± 0.1	1.108IleHis: 1.108 ± 0.042	8.942IleIle: 8.942 ± 0.168	8.623IleLys: 8.623 ± 0.111	9.405IleLeu: 9.405 ± 0.144	2.331IleMet: 2.331 ± 0.051	6.196IleAsn: 6.196 ± 0.092	3.116IlePro: 3.116 ± 0.068	1.862IleGln: 1.862 ± 0.044	2.919IleArg: 2.919 ± 0.061	6.533IleSer: 6.533 ± 0.097	4.466IleThr: 4.466 ± 0.074	6.173IleVal: 6.173 ± 0.091	0.556IleTrp: 0.556 ± 0.029	3.468IleTyr: 3.468 ± 0.065	0.0IleXaa: 0.0 ± 0.0
Lys	5.002LysAla: 5.002 ± 0.077	0.882LysCys: 0.882 ± 0.036	6.467LysAsp: 6.467 ± 0.097	10.637LysGlu: 10.637 ± 0.157	3.537LysPhe: 3.537 ± 0.066	5.548LysGly: 5.548 ± 0.075	1.066LysHis: 1.066 ± 0.037	8.124LysIle: 8.124 ± 0.111	8.512LysLys: 8.512 ± 0.125	7.455LysLeu: 7.455 ± 0.105	2.261LysMet: 2.261 ± 0.049	6.482LysAsn: 6.482 ± 0.094	2.107LysPro: 2.107 ± 0.055	1.674LysGln: 1.674 ± 0.054	3.058LysArg: 3.058 ± 0.064	5.173LysSer: 5.173 ± 0.092	3.917LysThr: 3.917 ± 0.083	6.492LysVal: 6.492 ± 0.1	0.599LysTrp: 0.599 ± 0.028	4.033LysTyr: 4.033 ± 0.071	0.0LysXaa: 0.0 ± 0.0
Leu	5.409LeuAla: 5.409 ± 0.103	1.075LeuCys: 1.075 ± 0.033	5.476LeuAsp: 5.476 ± 0.087	7.344LeuGlu: 7.344 ± 0.119	3.777LeuPhe: 3.777 ± 0.076	6.869LeuGly: 6.869 ± 0.101	1.036LeuHis: 1.036 ± 0.032	8.582LeuIle: 8.582 ± 0.136	9.003LeuLys: 9.003 ± 0.118	7.81LeuLeu: 7.81 ± 0.114	2.323LeuMet: 2.323 ± 0.061	6.446LeuAsn: 6.446 ± 0.094	2.706LeuPro: 2.706 ± 0.057	1.729LeuGln: 1.729 ± 0.046	3.298LeuArg: 3.298 ± 0.071	6.424LeuSer: 6.424 ± 0.101	4.482LeuThr: 4.482 ± 0.079	5.857LeuVal: 5.857 ± 0.089	0.583LeuTrp: 0.583 ± 0.032	3.164LeuTyr: 3.164 ± 0.07	0.0LeuXaa: 0.0 ± 0.0
Met	1.79MetAla: 1.79 ± 0.055	0.259MetCys: 0.259 ± 0.017	1.432MetAsp: 1.432 ± 0.041	1.808MetGlu: 1.808 ± 0.045	1.052MetPhe: 1.052 ± 0.036	1.792MetGly: 1.792 ± 0.052	0.325MetHis: 0.325 ± 0.02	2.137MetIle: 2.137 ± 0.052	2.454MetLys: 2.454 ± 0.052	2.117MetLeu: 2.117 ± 0.051	0.595MetMet: 0.595 ± 0.028	1.736MetAsn: 1.736 ± 0.045	0.83MetPro: 0.83 ± 0.034	0.568MetGln: 0.568 ± 0.03	0.932MetArg: 0.932 ± 0.029	1.619MetSer: 1.619 ± 0.046	1.098MetThr: 1.098 ± 0.035	1.652MetVal: 1.652 ± 0.047	0.181MetTrp: 0.181 ± 0.016	0.82MetTyr: 0.82 ± 0.033	0.0MetXaa: 0.0 ± 0.0
Asn	2.711AsnAla: 2.711 ± 0.059	0.677AsnCys: 0.677 ± 0.032	3.088AsnAsp: 3.088 ± 0.067	4.755AsnGlu: 4.755 ± 0.087	2.946AsnPhe: 2.946 ± 0.068	4.081AsnGly: 4.081 ± 0.091	0.827AsnHis: 0.827 ± 0.036	6.845AsnIle: 6.845 ± 0.093	6.683AsnLys: 6.683 ± 0.095	6.159AsnLeu: 6.159 ± 0.103	1.639AsnMet: 1.639 ± 0.042	4.687AsnAsn: 4.687 ± 0.111	2.235AsnPro: 2.235 ± 0.056	1.229AsnGln: 1.229 ± 0.038	2.011AsnArg: 2.011 ± 0.046	4.207AsnSer: 4.207 ± 0.09	2.807AsnThr: 2.807 ± 0.069	3.383AsnVal: 3.383 ± 0.072	0.469AsnTrp: 0.469 ± 0.025	2.541AsnTyr: 2.541 ± 0.063	0.0AsnXaa: 0.0 ± 0.0
Pro	1.368ProAla: 1.368 ± 0.044	0.301ProCys: 0.301 ± 0.017	1.299ProAsp: 1.299 ± 0.039	2.304ProGlu: 2.304 ± 0.058	1.316ProPhe: 1.316 ± 0.038	1.763ProGly: 1.763 ± 0.049	0.46ProHis: 0.46 ± 0.023	2.531ProIle: 2.531 ± 0.057	2.303ProLys: 2.303 ± 0.061	2.428ProLeu: 2.428 ± 0.06	0.748ProMet: 0.748 ± 0.032	1.654ProAsn: 1.654 ± 0.049	0.512ProPro: 0.512 ± 0.022	0.714ProGln: 0.714 ± 0.038	0.824ProArg: 0.824 ± 0.031	1.757ProSer: 1.757 ± 0.051	1.401ProThr: 1.401 ± 0.042	2.042ProVal: 2.042 ± 0.051	0.251ProTrp: 0.251 ± 0.016	1.27ProTyr: 1.27 ± 0.04	0.0ProXaa: 0.0 ± 0.0
Gln	1.181GlnAla: 1.181 ± 0.039	0.268GlnCys: 0.268 ± 0.017	1.006GlnAsp: 1.006 ± 0.035	1.598GlnGlu: 1.598 ± 0.037	0.882GlnPhe: 0.882 ± 0.034	1.442GlnGly: 1.442 ± 0.052	0.224GlnHis: 0.224 ± 0.017	1.72GlnIle: 1.72 ± 0.046	1.728GlnLys: 1.728 ± 0.045	1.661GlnLeu: 1.661 ± 0.046	0.534GlnMet: 0.534 ± 0.026	1.195GlnAsn: 1.195 ± 0.036	0.498GlnPro: 0.498 ± 0.024	0.44GlnGln: 0.44 ± 0.024	0.848GlnArg: 0.848 ± 0.04	1.202GlnSer: 1.202 ± 0.043	0.814GlnThr: 0.814 ± 0.034	1.355GlnVal: 1.355 ± 0.045	0.227GlnTrp: 0.227 ± 0.018	0.861GlnTyr: 0.861 ± 0.035	0.0GlnXaa: 0.0 ± 0.0
Arg	1.844ArgAla: 1.844 ± 0.05	0.386ArgCys: 0.386 ± 0.022	1.944ArgAsp: 1.944 ± 0.056	3.418ArgGlu: 3.418 ± 0.071	1.499ArgPhe: 1.499 ± 0.048	2.045ArgGly: 2.045 ± 0.049	0.424ArgHis: 0.424 ± 0.024	3.196ArgIle: 3.196 ± 0.065	2.978ArgLys: 2.978 ± 0.059	2.856ArgLeu: 2.856 ± 0.059	0.935ArgMet: 0.935 ± 0.034	2.033ArgAsn: 2.033 ± 0.049	0.821ArgPro: 0.821 ± 0.033	0.705ArgGln: 0.705 ± 0.03	1.359ArgArg: 1.359 ± 0.049	1.528ArgSer: 1.528 ± 0.048	1.443ArgThr: 1.443 ± 0.042	2.37ArgVal: 2.37 ± 0.056	0.245ArgTrp: 0.245 ± 0.016	1.316ArgTyr: 1.316 ± 0.045	0.0ArgXaa: 0.0 ± 0.0
Ser	2.949SerAla: 2.949 ± 0.061	0.578SerCys: 0.578 ± 0.03	2.982SerAsp: 2.982 ± 0.072	4.23SerGlu: 4.23 ± 0.08	3.156SerPhe: 3.156 ± 0.063	4.082SerGly: 4.082 ± 0.075	0.866SerHis: 0.866 ± 0.032	6.151SerIle: 6.151 ± 0.09	6.086SerLys: 6.086 ± 0.089	6.542SerLeu: 6.542 ± 0.095	1.58SerMet: 1.58 ± 0.038	3.898SerAsn: 3.898 ± 0.092	1.629SerPro: 1.629 ± 0.043	1.447SerGln: 1.447 ± 0.042	1.945SerArg: 1.945 ± 0.048	4.202SerSer: 4.202 ± 0.092	2.955SerThr: 2.955 ± 0.055	3.717SerVal: 3.717 ± 0.064	0.486SerTrp: 0.486 ± 0.027	2.603SerTyr: 2.603 ± 0.064	0.0SerXaa: 0.0 ± 0.0
Thr	2.607ThrAla: 2.607 ± 0.063	0.499ThrCys: 0.499 ± 0.026	2.178ThrAsp: 2.178 ± 0.055	2.889ThrGlu: 2.889 ± 0.063	2.24ThrPhe: 2.24 ± 0.059	3.565ThrGly: 3.565 ± 0.076	0.715ThrHis: 0.715 ± 0.029	4.366ThrIle: 4.366 ± 0.073	3.517ThrLys: 3.517 ± 0.067	5.138ThrLeu: 5.138 ± 0.084	1.096ThrMet: 1.096 ± 0.033	2.442ThrAsn: 2.442 ± 0.049	1.815ThrPro: 1.815 ± 0.045	0.896ThrGln: 0.896 ± 0.033	1.464ThrArg: 1.464 ± 0.041	2.996ThrSer: 2.996 ± 0.066	2.312ThrThr: 2.312 ± 0.055	3.152ThrVal: 3.152 ± 0.066	0.38ThrTrp: 0.38 ± 0.02	1.861ThrTyr: 1.861 ± 0.047	0.0ThrXaa: 0.0 ± 0.0
Val	3.848ValAla: 3.848 ± 0.078	0.842ValCys: 0.842 ± 0.029	3.781ValAsp: 3.781 ± 0.071	4.743ValGlu: 4.743 ± 0.083	2.915ValPhe: 2.915 ± 0.059	4.398ValGly: 4.398 ± 0.086	0.847ValHis: 0.847 ± 0.03	6.117ValIle: 6.117 ± 0.093	5.745ValLys: 5.745 ± 0.095	6.376ValLeu: 6.376 ± 0.087	1.458ValMet: 1.458 ± 0.044	3.817ValAsn: 3.817 ± 0.072	1.996ValPro: 1.996 ± 0.052	1.347ValGln: 1.347 ± 0.046	2.019ValArg: 2.019 ± 0.041	4.463ValSer: 4.463 ± 0.079	3.33ValThr: 3.33 ± 0.074	4.467ValVal: 4.467 ± 0.08	0.374ValTrp: 0.374 ± 0.023	2.49ValTyr: 2.49 ± 0.058	0.0ValXaa: 0.0 ± 0.0
Trp	0.4TrpAla: 0.4 ± 0.019	0.093TrpCys: 0.093 ± 0.011	0.374TrpAsp: 0.374 ± 0.023	0.445TrpGlu: 0.445 ± 0.028	0.327TrpPhe: 0.327 ± 0.022	0.507TrpGly: 0.507 ± 0.027	0.109TrpHis: 0.109 ± 0.012	0.672TrpIle: 0.672 ± 0.035	0.519TrpLys: 0.519 ± 0.026	0.582TrpLeu: 0.582 ± 0.029	0.164TrpMet: 0.164 ± 0.015	0.479TrpAsn: 0.479 ± 0.027	0.146TrpPro: 0.146 ± 0.014	0.211TrpGln: 0.211 ± 0.02	0.277TrpArg: 0.277 ± 0.02	0.41TrpSer: 0.41 ± 0.023	0.389TrpThr: 0.389 ± 0.024	0.433TrpVal: 0.433 ± 0.024	0.069TrpTrp: 0.069 ± 0.009	0.337TrpTyr: 0.337 ± 0.033	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.854TyrAla: 1.854 ± 0.047	0.486TyrCys: 0.486 ± 0.023	2.413TyrAsp: 2.413 ± 0.052	3.113TyrGlu: 3.113 ± 0.058	2.046TyrPhe: 2.046 ± 0.061	2.564TyrGly: 2.564 ± 0.057	0.533TyrHis: 0.533 ± 0.026	3.773TyrIle: 3.773 ± 0.07	3.782TyrLys: 3.782 ± 0.078	3.759TyrLeu: 3.759 ± 0.075	0.967TyrMet: 0.967 ± 0.035	2.573TyrAsn: 2.573 ± 0.064	1.216TyrPro: 1.216 ± 0.044	0.624TyrGln: 0.624 ± 0.028	1.369TyrArg: 1.369 ± 0.043	2.762TyrSer: 2.762 ± 0.056	1.829TyrThr: 1.829 ± 0.044	2.343TyrVal: 2.343 ± 0.059	0.287TyrTrp: 0.287 ± 0.019	1.831TyrTyr: 1.831 ± 0.055	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2721 proteins (855859 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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