Pseudomonas phage YMC11/02/R656

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 113 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S2SY83|A0A0S2SY83_9CAUD Uncharacterized protein OS=Pseudomonas phage YMC11/02/R656 OX=1755689 GN=BPPAER656_00280 PE=4 SV=1
MM1 pKa = 6.54TQRR4 pKa = 11.84ILFFTAGPALTEE16 pKa = 4.07AEE18 pKa = 4.21KK19 pKa = 11.13SVVEE23 pKa = 3.96QLNALTPPTYY33 pKa = 10.74SVTVHH38 pKa = 6.26NGAVEE43 pKa = 3.85PDD45 pKa = 3.69YY46 pKa = 11.27VVPGDD51 pKa = 3.85YY52 pKa = 10.75AAGAIPQAYY61 pKa = 9.85AGLPVFDD68 pKa = 5.76PDD70 pKa = 4.12NPPAPAVGEE79 pKa = 4.25GQVVVSNDD87 pKa = 2.82ATVSVLPASGSMALASGTADD107 pKa = 3.3VASGALAGIRR117 pKa = 11.84LAATAAVVANGQTISVAGGTVTLSVAANVVTAVFTPEE154 pKa = 3.4

Molecular weight: 15.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S2SY55|A0A0S2SY55_9CAUD Uncharacterized protein OS=Pseudomonas phage YMC11/02/R656 OX=1755689 GN=BPPAER656_00060 PE=4 SV=1
MM1 pKa = 7.42PAAASTEE8 pKa = 4.08IKK10 pKa = 10.47DD11 pKa = 4.02IARR14 pKa = 11.84PWPINGIGLAVRR26 pKa = 11.84IRR28 pKa = 11.84GGLAGQGARR37 pKa = 11.84IGQYY41 pKa = 10.64GGG43 pKa = 3.24

Molecular weight: 4.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

113

0

113

17840

37

1185

157.9

17.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.592 ± 0.503

1.278 ± 0.13

5.684 ± 0.186

7.27 ± 0.337

3.189 ± 0.167

7.556 ± 0.273

1.855 ± 0.179

4.989 ± 0.168

4.417 ± 0.23

8.335 ± 0.251

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.472 ± 0.15

3.178 ± 0.181

4.266 ± 0.239

4.568 ± 0.285

7.209 ± 0.255

6.054 ± 0.259

4.871 ± 0.255

6.715 ± 0.235

1.721 ± 0.149

2.78 ± 0.161
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski