Leifsonia sp. Root227

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Leifsonia; unclassified Leifsonia

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3674 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q8V9X5|A0A0Q8V9X5_9MICO Uncharacterized protein OS=Leifsonia sp. Root227 OX=1736496 GN=ASE16_06970 PE=4 SV=1
MM1 pKa = 8.04DD2 pKa = 5.84ALTRR6 pKa = 11.84TIKK9 pKa = 10.66AALLYY14 pKa = 10.28EE15 pKa = 4.91DD16 pKa = 5.65GVTLDD21 pKa = 3.6SVVIVPLIGGCPPGEE36 pKa = 3.64IRR38 pKa = 11.84VATTFGGALAYY49 pKa = 9.85DD50 pKa = 4.4VYY52 pKa = 11.31EE53 pKa = 5.15LDD55 pKa = 4.35DD56 pKa = 4.88DD57 pKa = 4.84EE58 pKa = 5.65EE59 pKa = 4.55FSSMPAYY66 pKa = 9.46PYY68 pKa = 10.56KK69 pKa = 10.42ATVPVTTADD78 pKa = 4.26LDD80 pKa = 4.05DD81 pKa = 4.06

Molecular weight: 8.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q8UYX9|A0A0Q8UYX9_9MICO 2-dehydropantoate 2-reductase OS=Leifsonia sp. Root227 OX=1736496 GN=ASE16_05930 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84KK12 pKa = 8.98RR13 pKa = 11.84AKK15 pKa = 8.46THH17 pKa = 5.11GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 11.04GRR40 pKa = 11.84TKK42 pKa = 10.91LSAA45 pKa = 3.61

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3674

0

3674

1184469

27

2105

322.4

34.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.478 ± 0.057

0.463 ± 0.009

6.128 ± 0.034

5.182 ± 0.041

3.312 ± 0.026

8.92 ± 0.033

1.887 ± 0.019

4.731 ± 0.032

2.279 ± 0.032

10.091 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.712 ± 0.016

2.184 ± 0.022

5.258 ± 0.035

2.904 ± 0.02

6.715 ± 0.042

5.905 ± 0.027

6.266 ± 0.034

9.016 ± 0.043

1.498 ± 0.016

2.072 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski