Turnip rosette virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Sobemovirus

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S4Z703|S4Z703_9VIRU N-terminal protein OS=Turnip rosette virus OX=218923 PE=4 SV=1
MM1 pKa = 7.2SRR3 pKa = 11.84VATIEE8 pKa = 3.82IYY10 pKa = 10.63NEE12 pKa = 3.42NGIIVARR19 pKa = 11.84KK20 pKa = 7.5KK21 pKa = 9.37TSGPHH26 pKa = 6.22ALLEE30 pKa = 4.23LFNGKK35 pKa = 8.18QKK37 pKa = 10.76YY38 pKa = 9.55DD39 pKa = 3.44QVSEE43 pKa = 4.45LFVIWVCEE51 pKa = 4.03EE52 pKa = 4.0CGKK55 pKa = 8.43TVYY58 pKa = 8.96STCEE62 pKa = 3.36FKK64 pKa = 11.22GIVFVRR70 pKa = 11.84EE71 pKa = 4.01DD72 pKa = 3.39GKK74 pKa = 9.84EE75 pKa = 3.83TTEE78 pKa = 4.28FEE80 pKa = 4.25TEE82 pKa = 4.07AVVDD86 pKa = 5.15SDD88 pKa = 4.91DD89 pKa = 4.14CGCAYY94 pKa = 9.65EE95 pKa = 4.21YY96 pKa = 10.76HH97 pKa = 6.68SEE99 pKa = 4.31TEE101 pKa = 4.93SEE103 pKa = 4.24ACLCPGYY110 pKa = 10.38AIEE113 pKa = 6.16GICDD117 pKa = 3.66CDD119 pKa = 3.32WYY121 pKa = 10.58EE122 pKa = 4.96DD123 pKa = 3.82RR124 pKa = 11.84PEE126 pKa = 4.2TSDD129 pKa = 3.32SSEE132 pKa = 5.88LFTQWEE138 pKa = 4.12RR139 pKa = 11.84LEE141 pKa = 4.54LFSDD145 pKa = 4.09

Molecular weight: 16.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S4YZ53|S4YZ53_9VIRU N-terminal protein OS=Turnip rosette virus OX=218923 PE=4 SV=1
MM1 pKa = 7.57EE2 pKa = 5.84KK3 pKa = 10.76GNKK6 pKa = 9.45KK7 pKa = 8.23LTKK10 pKa = 8.21NQRR13 pKa = 11.84RR14 pKa = 11.84KK15 pKa = 7.18TRR17 pKa = 11.84KK18 pKa = 9.4KK19 pKa = 8.98NSQSGRR25 pKa = 11.84TNSVVTPLWAPVSTGTVMQGGALSISSLNNKK56 pKa = 9.39GDD58 pKa = 3.05IRR60 pKa = 11.84VCGRR64 pKa = 11.84EE65 pKa = 3.91VVTEE69 pKa = 3.9ISVSNTPTPSVLQVIPEE86 pKa = 4.52TFPSRR91 pKa = 11.84LKK93 pKa = 11.12GLATSWSKK101 pKa = 10.28FKK103 pKa = 9.86WQAIKK108 pKa = 10.06FVYY111 pKa = 9.75MPICPTTEE119 pKa = 3.63RR120 pKa = 11.84GSVHH124 pKa = 7.23FGFLYY129 pKa = 9.55DD130 pKa = 4.11TVDD133 pKa = 3.64NLPGTVGEE141 pKa = 4.18ISTLQGYY148 pKa = 6.09TTGSVWAGTSGNEE161 pKa = 3.88LLEE164 pKa = 5.16DD165 pKa = 3.52GVYY168 pKa = 11.03AKK170 pKa = 9.69TPKK173 pKa = 10.15DD174 pKa = 3.38AVVARR179 pKa = 11.84MDD181 pKa = 4.02ARR183 pKa = 11.84RR184 pKa = 11.84ADD186 pKa = 3.28KK187 pKa = 10.27KK188 pKa = 10.65YY189 pKa = 10.12YY190 pKa = 9.64PIVSTTQLTKK200 pKa = 10.76SLNVDD205 pKa = 3.11ASLGNTYY212 pKa = 10.23VPARR216 pKa = 11.84LAILTADD223 pKa = 3.7GTTAADD229 pKa = 3.57KK230 pKa = 9.88PQIVGRR236 pKa = 11.84LYY238 pKa = 11.1AVFCVDD244 pKa = 6.16LIDD247 pKa = 5.18TIASSLNVV255 pKa = 3.33

Molecular weight: 27.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1867

145

929

466.8

51.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.999 ± 0.23

1.768 ± 0.715

4.66 ± 0.383

7.445 ± 1.404

3.16 ± 0.491

7.177 ± 0.169

1.821 ± 0.304

4.928 ± 0.103

5.785 ± 0.265

8.784 ± 0.617

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.5 ± 0.134

3.642 ± 0.267

4.66 ± 0.411

2.946 ± 0.211

5.731 ± 0.431

9.373 ± 0.758

7.177 ± 1.04

8.784 ± 0.306

1.875 ± 0.196

2.785 ± 0.428
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski