Vibrio phage Ceto

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Demerecviridae; Ermolyevavirinae; Cetovirus; Vibrio virus Ceto

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 181 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2H5BGR2|A0A2H5BGR2_9CAUD Macro domain-containing protein OS=Vibrio phage Ceto OX=2570300 GN=CETO_176 PE=4 SV=1
MM1 pKa = 7.75ADD3 pKa = 3.23FWDD6 pKa = 3.36KK7 pKa = 11.22HH8 pKa = 4.61LTKK11 pKa = 9.82EE12 pKa = 3.97QQYY15 pKa = 10.59EE16 pKa = 4.19IVDD19 pKa = 4.28SVAGTCDD26 pKa = 3.11TGCNAEE32 pKa = 4.07YY33 pKa = 8.82WQKK36 pKa = 11.08EE37 pKa = 3.99FGFSNEE43 pKa = 4.33VRR45 pKa = 11.84DD46 pKa = 3.92EE47 pKa = 4.47LNWDD51 pKa = 4.02FPSLAAKK58 pKa = 10.51FEE60 pKa = 4.34LFRR63 pKa = 11.84CEE65 pKa = 4.38DD66 pKa = 3.84CMWWGDD72 pKa = 3.46MGEE75 pKa = 4.25VGDD78 pKa = 4.54CNEE81 pKa = 4.77CGGEE85 pKa = 4.11LVV87 pKa = 3.95

Molecular weight:
10.08 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2H5BGM5|A0A2H5BGM5_9CAUD Uncharacterized protein OS=Vibrio phage Ceto OX=2570300 GN=CETO_165 PE=4 SV=1
MM1 pKa = 8.01DD2 pKa = 4.66RR3 pKa = 11.84RR4 pKa = 11.84HH5 pKa = 6.23KK6 pKa = 11.02LRR8 pKa = 11.84VNWHH12 pKa = 6.27RR13 pKa = 11.84RR14 pKa = 11.84LKK16 pKa = 10.67DD17 pKa = 3.35DD18 pKa = 5.35EE19 pKa = 4.14IAIEE23 pKa = 4.07NGFTTRR29 pKa = 11.84LFKK32 pKa = 9.91QTEE35 pKa = 4.38VYY37 pKa = 9.9MYY39 pKa = 10.76DD40 pKa = 3.98LSWWDD45 pKa = 3.37YY46 pKa = 10.54PRR48 pKa = 11.84RR49 pKa = 11.84TLEE52 pKa = 4.11RR53 pKa = 11.84SWKK56 pKa = 8.9NQSKK60 pKa = 10.1KK61 pKa = 9.47RR62 pKa = 11.84KK63 pKa = 6.39QWEE66 pKa = 3.91RR67 pKa = 3.3

Molecular weight:
8.71 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

181

0

181

36038

35

1018

199.1

22.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.678 ± 0.356

1.246 ± 0.096

6.299 ± 0.144

6.92 ± 0.212

4.082 ± 0.146

6.396 ± 0.173

2.048 ± 0.116

6.26 ± 0.15

7.437 ± 0.151

8.585 ± 0.186

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.594 ± 0.093

5.025 ± 0.14

3.535 ± 0.141

3.605 ± 0.127

4.512 ± 0.15

6.008 ± 0.23

6.096 ± 0.188

6.313 ± 0.156

1.423 ± 0.083

3.938 ± 0.136

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski