Amino acid dipepetide frequency for Escherichia phage ESSI2_ev129

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.764AlaAla: 12.764 ± 1.437
0.618AlaCys: 0.618 ± 0.267
5.558AlaAsp: 5.558 ± 0.803
6.485AlaGlu: 6.485 ± 0.648
3.911AlaPhe: 3.911 ± 0.545
8.749AlaGly: 8.749 ± 1.025
3.397AlaHis: 3.397 ± 1.057
5.455AlaIle: 5.455 ± 0.649
4.323AlaLys: 4.323 ± 0.581
8.955AlaLeu: 8.955 ± 1.261
3.191AlaMet: 3.191 ± 0.591
3.809AlaAsn: 3.809 ± 0.624
3.294AlaPro: 3.294 ± 0.68
4.117AlaGln: 4.117 ± 0.767
5.867AlaArg: 5.867 ± 0.598
6.794AlaSer: 6.794 ± 0.895
5.661AlaThr: 5.661 ± 0.734
8.338AlaVal: 8.338 ± 1.011
1.853AlaTrp: 1.853 ± 0.422
2.059AlaTyr: 2.059 ± 0.431
0.0AlaXaa: 0.0 ± 0.0
Cys
1.338CysAla: 1.338 ± 0.359
0.309CysCys: 0.309 ± 0.19
0.309CysAsp: 0.309 ± 0.165
0.412CysGlu: 0.412 ± 0.207
0.206CysPhe: 0.206 ± 0.138
0.926CysGly: 0.926 ± 0.331
0.103CysHis: 0.103 ± 0.123
0.206CysIle: 0.206 ± 0.155
0.103CysLys: 0.103 ± 0.112
0.515CysLeu: 0.515 ± 0.228
0.103CysMet: 0.103 ± 0.098
0.515CysAsn: 0.515 ± 0.264
0.515CysPro: 0.515 ± 0.205
0.206CysGln: 0.206 ± 0.136
0.721CysArg: 0.721 ± 0.34
0.926CysSer: 0.926 ± 0.405
0.721CysThr: 0.721 ± 0.327
0.823CysVal: 0.823 ± 0.272
0.309CysTrp: 0.309 ± 0.195
0.412CysTyr: 0.412 ± 0.193
0.0CysXaa: 0.0 ± 0.0
Asp
5.867AspAla: 5.867 ± 0.848
0.412AspCys: 0.412 ± 0.246
2.162AspAsp: 2.162 ± 0.48
4.529AspGlu: 4.529 ± 0.708
1.75AspPhe: 1.75 ± 0.427
4.323AspGly: 4.323 ± 0.781
0.515AspHis: 0.515 ± 0.233
3.5AspIle: 3.5 ± 0.7
2.779AspLys: 2.779 ± 0.519
4.941AspLeu: 4.941 ± 0.715
1.235AspMet: 1.235 ± 0.297
1.75AspAsn: 1.75 ± 0.427
1.853AspPro: 1.853 ± 0.558
1.75AspGln: 1.75 ± 0.337
2.162AspArg: 2.162 ± 0.359
3.088AspSer: 3.088 ± 0.621
3.088AspThr: 3.088 ± 0.56
5.147AspVal: 5.147 ± 0.702
1.132AspTrp: 1.132 ± 0.342
2.985AspTyr: 2.985 ± 0.709
0.0AspXaa: 0.0 ± 0.0
Glu
6.073GluAla: 6.073 ± 0.846
0.823GluCys: 0.823 ± 0.411
3.088GluAsp: 3.088 ± 0.738
3.088GluGlu: 3.088 ± 0.632
1.853GluPhe: 1.853 ± 0.383
3.088GluGly: 3.088 ± 0.531
1.338GluHis: 1.338 ± 0.379
4.22GluIle: 4.22 ± 0.695
3.397GluLys: 3.397 ± 0.581
6.382GluLeu: 6.382 ± 0.799
2.367GluMet: 2.367 ± 0.436
3.603GluAsn: 3.603 ± 0.699
2.367GluPro: 2.367 ± 0.481
2.47GluGln: 2.47 ± 0.392
3.706GluArg: 3.706 ± 0.685
3.191GluSer: 3.191 ± 0.605
3.706GluThr: 3.706 ± 0.574
3.911GluVal: 3.911 ± 0.684
1.441GluTrp: 1.441 ± 0.452
1.235GluTyr: 1.235 ± 0.399
0.0GluXaa: 0.0 ± 0.0
Phe
4.22PheAla: 4.22 ± 0.57
0.103PheCys: 0.103 ± 0.098
2.985PheAsp: 2.985 ± 0.482
2.162PheGlu: 2.162 ± 0.512
1.441PhePhe: 1.441 ± 0.456
2.676PheGly: 2.676 ± 0.459
0.412PheHis: 0.412 ± 0.245
1.956PheIle: 1.956 ± 0.468
1.132PheLys: 1.132 ± 0.266
1.853PheLeu: 1.853 ± 0.333
1.235PheMet: 1.235 ± 0.388
1.853PheAsn: 1.853 ± 0.512
1.235PhePro: 1.235 ± 0.357
1.132PheGln: 1.132 ± 0.374
1.132PheArg: 1.132 ± 0.317
3.5PheSer: 3.5 ± 0.507
2.573PheThr: 2.573 ± 0.548
2.573PheVal: 2.573 ± 0.448
1.029PheTrp: 1.029 ± 0.372
0.823PheTyr: 0.823 ± 0.291
0.0PheXaa: 0.0 ± 0.0
Gly
8.132GlyAla: 8.132 ± 1.306
0.721GlyCys: 0.721 ± 0.279
4.323GlyAsp: 4.323 ± 0.892
5.25GlyGlu: 5.25 ± 0.913
3.294GlyPhe: 3.294 ± 0.6
6.691GlyGly: 6.691 ± 0.951
1.029GlyHis: 1.029 ± 0.342
4.22GlyIle: 4.22 ± 0.679
4.838GlyLys: 4.838 ± 0.889
4.529GlyLeu: 4.529 ± 0.786
2.162GlyMet: 2.162 ± 0.505
4.838GlyAsn: 4.838 ± 0.74
0.103GlyPro: 0.103 ± 0.106
5.044GlyGln: 5.044 ± 0.547
4.014GlyArg: 4.014 ± 0.627
4.014GlySer: 4.014 ± 0.734
3.809GlyThr: 3.809 ± 0.66
4.632GlyVal: 4.632 ± 0.828
1.441GlyTrp: 1.441 ± 0.319
2.265GlyTyr: 2.265 ± 0.494
0.0GlyXaa: 0.0 ± 0.0
His
1.441HisAla: 1.441 ± 0.454
0.103HisCys: 0.103 ± 0.104
0.721HisAsp: 0.721 ± 0.222
1.338HisGlu: 1.338 ± 0.367
0.926HisPhe: 0.926 ± 0.227
0.721HisGly: 0.721 ± 0.268
0.412HisHis: 0.412 ± 0.247
0.823HisIle: 0.823 ± 0.298
0.412HisLys: 0.412 ± 0.192
2.162HisLeu: 2.162 ± 0.513
0.618HisMet: 0.618 ± 0.345
0.926HisAsn: 0.926 ± 0.302
0.721HisPro: 0.721 ± 0.266
0.309HisGln: 0.309 ± 0.173
0.823HisArg: 0.823 ± 0.259
2.059HisSer: 2.059 ± 0.903
3.294HisThr: 3.294 ± 1.212
1.029HisVal: 1.029 ± 0.325
0.412HisTrp: 0.412 ± 0.202
0.823HisTyr: 0.823 ± 0.306
0.0HisXaa: 0.0 ± 0.0
Ile
7.308IleAla: 7.308 ± 0.766
0.309IleCys: 0.309 ± 0.158
2.47IleAsp: 2.47 ± 0.451
3.5IleGlu: 3.5 ± 0.62
1.75IlePhe: 1.75 ± 0.467
4.323IleGly: 4.323 ± 0.993
1.132IleHis: 1.132 ± 0.356
2.059IleIle: 2.059 ± 0.592
2.676IleLys: 2.676 ± 0.678
2.882IleLeu: 2.882 ± 0.643
0.823IleMet: 0.823 ± 0.337
1.853IleAsn: 1.853 ± 0.458
2.573IlePro: 2.573 ± 0.478
1.956IleGln: 1.956 ± 0.488
3.809IleArg: 3.809 ± 0.497
4.117IleSer: 4.117 ± 0.663
4.22IleThr: 4.22 ± 0.648
2.882IleVal: 2.882 ± 0.529
0.721IleTrp: 0.721 ± 0.254
1.853IleTyr: 1.853 ± 0.454
0.0IleXaa: 0.0 ± 0.0
Lys
6.073LysAla: 6.073 ± 0.889
0.309LysCys: 0.309 ± 0.224
1.853LysAsp: 1.853 ± 0.425
2.47LysGlu: 2.47 ± 0.519
1.956LysPhe: 1.956 ± 0.409
3.603LysGly: 3.603 ± 0.559
0.823LysHis: 0.823 ± 0.285
2.367LysIle: 2.367 ± 0.557
2.985LysLys: 2.985 ± 0.613
3.911LysLeu: 3.911 ± 0.7
1.853LysMet: 1.853 ± 0.562
2.573LysAsn: 2.573 ± 0.624
3.294LysPro: 3.294 ± 0.601
2.059LysGln: 2.059 ± 0.537
3.294LysArg: 3.294 ± 0.697
3.5LysSer: 3.5 ± 0.67
4.014LysThr: 4.014 ± 0.658
2.676LysVal: 2.676 ± 0.505
1.132LysTrp: 1.132 ± 0.328
2.162LysTyr: 2.162 ± 0.494
0.0LysXaa: 0.0 ± 0.0
Leu
10.602LeuAla: 10.602 ± 1.046
1.544LeuCys: 1.544 ± 0.379
4.632LeuAsp: 4.632 ± 0.752
4.941LeuGlu: 4.941 ± 0.755
2.367LeuPhe: 2.367 ± 0.436
5.764LeuGly: 5.764 ± 0.774
1.544LeuHis: 1.544 ± 0.378
3.5LeuIle: 3.5 ± 0.671
5.764LeuLys: 5.764 ± 1.066
8.235LeuLeu: 8.235 ± 0.983
1.544LeuMet: 1.544 ± 0.401
4.632LeuAsn: 4.632 ± 0.582
4.426LeuPro: 4.426 ± 0.708
2.573LeuGln: 2.573 ± 0.588
6.588LeuArg: 6.588 ± 0.704
5.558LeuSer: 5.558 ± 0.942
5.455LeuThr: 5.455 ± 0.852
5.558LeuVal: 5.558 ± 0.639
0.618LeuTrp: 0.618 ± 0.285
2.676LeuTyr: 2.676 ± 0.566
0.0LeuXaa: 0.0 ± 0.0
Met
3.911MetAla: 3.911 ± 0.818
0.206MetCys: 0.206 ± 0.138
0.823MetAsp: 0.823 ± 0.291
1.338MetGlu: 1.338 ± 0.35
0.618MetPhe: 0.618 ± 0.22
1.132MetGly: 1.132 ± 0.441
0.309MetHis: 0.309 ± 0.168
1.338MetIle: 1.338 ± 0.401
1.338MetLys: 1.338 ± 0.397
2.162MetLeu: 2.162 ± 0.348
0.926MetMet: 0.926 ± 0.336
1.029MetAsn: 1.029 ± 0.297
1.029MetPro: 1.029 ± 0.375
1.544MetGln: 1.544 ± 0.395
1.956MetArg: 1.956 ± 0.432
1.544MetSer: 1.544 ± 0.354
1.853MetThr: 1.853 ± 0.416
1.235MetVal: 1.235 ± 0.354
0.515MetTrp: 0.515 ± 0.215
0.309MetTyr: 0.309 ± 0.172
0.0MetXaa: 0.0 ± 0.0
Asn
4.323AsnAla: 4.323 ± 0.576
0.721AsnCys: 0.721 ± 0.254
3.191AsnAsp: 3.191 ± 0.613
2.985AsnGlu: 2.985 ± 0.583
1.75AsnPhe: 1.75 ± 0.421
4.22AsnGly: 4.22 ± 0.671
0.618AsnHis: 0.618 ± 0.264
2.779AsnIle: 2.779 ± 0.7
2.265AsnLys: 2.265 ± 0.598
4.117AsnLeu: 4.117 ± 0.516
1.544AsnMet: 1.544 ± 0.391
1.338AsnAsn: 1.338 ± 0.333
1.647AsnPro: 1.647 ± 0.418
1.75AsnGln: 1.75 ± 0.515
1.647AsnArg: 1.647 ± 0.448
1.853AsnSer: 1.853 ± 0.465
2.265AsnThr: 2.265 ± 0.421
1.647AsnVal: 1.647 ± 0.412
0.823AsnTrp: 0.823 ± 0.296
0.823AsnTyr: 0.823 ± 0.305
0.0AsnXaa: 0.0 ± 0.0
Pro
4.22ProAla: 4.22 ± 0.77
0.206ProCys: 0.206 ± 0.116
3.397ProAsp: 3.397 ± 0.583
3.397ProGlu: 3.397 ± 0.721
1.544ProPhe: 1.544 ± 0.374
2.676ProGly: 2.676 ± 0.525
0.823ProHis: 0.823 ± 0.289
1.544ProIle: 1.544 ± 0.488
1.441ProLys: 1.441 ± 0.389
3.088ProLeu: 3.088 ± 0.488
0.618ProMet: 0.618 ± 0.207
1.132ProAsn: 1.132 ± 0.299
1.441ProPro: 1.441 ± 0.343
1.647ProGln: 1.647 ± 0.443
2.265ProArg: 2.265 ± 0.484
2.367ProSer: 2.367 ± 0.441
1.441ProThr: 1.441 ± 0.461
4.323ProVal: 4.323 ± 0.689
0.618ProTrp: 0.618 ± 0.223
0.823ProTyr: 0.823 ± 0.297
0.0ProXaa: 0.0 ± 0.0
Gln
3.397GlnAla: 3.397 ± 0.754
0.618GlnCys: 0.618 ± 0.233
2.162GlnAsp: 2.162 ± 0.54
2.162GlnGlu: 2.162 ± 0.393
1.75GlnPhe: 1.75 ± 0.442
3.294GlnGly: 3.294 ± 0.437
0.721GlnHis: 0.721 ± 0.268
2.676GlnIle: 2.676 ± 0.508
2.265GlnLys: 2.265 ± 0.397
4.426GlnLeu: 4.426 ± 0.734
0.515GlnMet: 0.515 ± 0.253
1.132GlnAsn: 1.132 ± 0.34
1.338GlnPro: 1.338 ± 0.325
2.985GlnGln: 2.985 ± 0.73
3.088GlnArg: 3.088 ± 0.504
2.779GlnSer: 2.779 ± 0.562
2.779GlnThr: 2.779 ± 0.682
2.47GlnVal: 2.47 ± 0.545
0.412GlnTrp: 0.412 ± 0.195
1.132GlnTyr: 1.132 ± 0.317
0.0GlnXaa: 0.0 ± 0.0
Arg
4.117ArgAla: 4.117 ± 0.635
0.412ArgCys: 0.412 ± 0.284
3.5ArgAsp: 3.5 ± 0.788
2.985ArgGlu: 2.985 ± 0.761
2.676ArgPhe: 2.676 ± 0.538
2.882ArgGly: 2.882 ± 0.685
1.132ArgHis: 1.132 ± 0.367
3.397ArgIle: 3.397 ± 0.667
4.22ArgLys: 4.22 ± 0.742
6.999ArgLeu: 6.999 ± 0.76
1.853ArgMet: 1.853 ± 0.428
2.265ArgAsn: 2.265 ± 0.484
2.059ArgPro: 2.059 ± 0.467
2.779ArgGln: 2.779 ± 0.58
4.529ArgArg: 4.529 ± 1.254
3.5ArgSer: 3.5 ± 0.593
3.088ArgThr: 3.088 ± 0.6
3.088ArgVal: 3.088 ± 0.664
1.441ArgTrp: 1.441 ± 0.315
2.162ArgTyr: 2.162 ± 0.619
0.0ArgXaa: 0.0 ± 0.0
Ser
5.867SerAla: 5.867 ± 0.789
0.721SerCys: 0.721 ± 0.228
2.779SerAsp: 2.779 ± 0.51
4.014SerGlu: 4.014 ± 0.634
1.956SerPhe: 1.956 ± 0.599
6.588SerGly: 6.588 ± 0.796
1.853SerHis: 1.853 ± 0.716
2.882SerIle: 2.882 ± 0.539
4.014SerLys: 4.014 ± 0.699
6.485SerLeu: 6.485 ± 0.864
1.235SerMet: 1.235 ± 0.342
1.75SerAsn: 1.75 ± 0.511
1.956SerPro: 1.956 ± 0.44
2.676SerGln: 2.676 ± 0.463
3.191SerArg: 3.191 ± 0.625
1.853SerSer: 1.853 ± 0.506
2.985SerThr: 2.985 ± 0.524
4.632SerVal: 4.632 ± 0.65
1.132SerTrp: 1.132 ± 0.36
0.926SerTyr: 0.926 ± 0.279
0.0SerXaa: 0.0 ± 0.0
Thr
5.661ThrAla: 5.661 ± 0.827
0.618ThrCys: 0.618 ± 0.277
4.22ThrAsp: 4.22 ± 0.685
4.117ThrGlu: 4.117 ± 0.759
2.779ThrPhe: 2.779 ± 0.454
6.176ThrGly: 6.176 ± 0.922
1.75ThrHis: 1.75 ± 0.652
3.809ThrIle: 3.809 ± 0.545
2.779ThrLys: 2.779 ± 0.687
4.838ThrLeu: 4.838 ± 0.962
1.029ThrMet: 1.029 ± 0.471
1.544ThrAsn: 1.544 ± 0.336
3.088ThrPro: 3.088 ± 0.596
2.676ThrGln: 2.676 ± 0.456
2.676ThrArg: 2.676 ± 0.498
3.191ThrSer: 3.191 ± 0.581
3.603ThrThr: 3.603 ± 0.69
4.529ThrVal: 4.529 ± 0.936
1.338ThrTrp: 1.338 ± 0.372
1.235ThrTyr: 1.235 ± 0.413
0.0ThrXaa: 0.0 ± 0.0
Val
6.073ValAla: 6.073 ± 0.781
0.412ValCys: 0.412 ± 0.194
4.014ValAsp: 4.014 ± 0.476
4.529ValGlu: 4.529 ± 0.909
1.75ValPhe: 1.75 ± 0.473
4.426ValGly: 4.426 ± 0.802
1.441ValHis: 1.441 ± 0.446
4.117ValIle: 4.117 ± 0.756
3.706ValLys: 3.706 ± 1.004
6.382ValLeu: 6.382 ± 0.916
1.441ValMet: 1.441 ± 0.334
3.603ValAsn: 3.603 ± 0.582
3.5ValPro: 3.5 ± 0.495
2.265ValGln: 2.265 ± 0.512
3.088ValArg: 3.088 ± 0.452
2.985ValSer: 2.985 ± 0.546
4.941ValThr: 4.941 ± 0.771
4.014ValVal: 4.014 ± 0.502
1.853ValTrp: 1.853 ± 0.425
1.956ValTyr: 1.956 ± 0.458
0.0ValXaa: 0.0 ± 0.0
Trp
1.853TrpAla: 1.853 ± 0.602
0.206TrpCys: 0.206 ± 0.154
1.132TrpAsp: 1.132 ± 0.319
0.309TrpGlu: 0.309 ± 0.21
0.412TrpPhe: 0.412 ± 0.176
1.029TrpGly: 1.029 ± 0.381
0.206TrpHis: 0.206 ± 0.131
0.926TrpIle: 0.926 ± 0.286
1.235TrpLys: 1.235 ± 0.299
2.779TrpLeu: 2.779 ± 0.577
0.206TrpMet: 0.206 ± 0.133
0.823TrpAsn: 0.823 ± 0.302
1.441TrpPro: 1.441 ± 0.311
0.823TrpGln: 0.823 ± 0.247
2.367TrpArg: 2.367 ± 0.449
0.515TrpSer: 0.515 ± 0.254
0.515TrpThr: 0.515 ± 0.226
1.441TrpVal: 1.441 ± 0.336
0.515TrpTrp: 0.515 ± 0.218
0.618TrpTyr: 0.618 ± 0.207
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.956TyrAla: 1.956 ± 0.428
0.309TyrCys: 0.309 ± 0.174
1.647TyrAsp: 1.647 ± 0.346
1.338TyrGlu: 1.338 ± 0.386
1.338TyrPhe: 1.338 ± 0.437
2.059TyrGly: 2.059 ± 0.523
0.515TyrHis: 0.515 ± 0.261
1.441TyrIle: 1.441 ± 0.371
1.235TyrLys: 1.235 ± 0.412
2.882TyrLeu: 2.882 ± 0.55
0.515TyrMet: 0.515 ± 0.226
1.338TyrAsn: 1.338 ± 0.356
1.132TyrPro: 1.132 ± 0.282
1.441TyrGln: 1.441 ± 0.435
2.162TyrArg: 2.162 ± 0.509
2.162TyrSer: 2.162 ± 0.482
1.647TyrThr: 1.647 ± 0.349
1.544TyrVal: 1.544 ± 0.382
0.618TyrTrp: 0.618 ± 0.274
0.823TyrTyr: 0.823 ± 0.257
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 42 proteins (9716 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski