Aminobacter phage Erebus

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 90 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3T7Z9|A0A6M3T7Z9_9CAUD 7-carboxy-7-deazaguanine synthase OS=Aminobacter phage Erebus OX=2686310 PE=3 SV=1
MM1 pKa = 7.51KK2 pKa = 9.86CDD4 pKa = 3.94KK5 pKa = 10.45DD6 pKa = 4.13GSMSNSEE13 pKa = 3.98LKK15 pKa = 10.78KK16 pKa = 10.56IVFDD20 pKa = 4.03NLDD23 pKa = 3.38AAKK26 pKa = 10.33EE27 pKa = 3.95NGYY30 pKa = 10.08FEE32 pKa = 5.62PGEE35 pKa = 3.99QLHH38 pKa = 6.42GSSAEE43 pKa = 4.51DD44 pKa = 3.09IALDD48 pKa = 4.93LIAFACDD55 pKa = 3.95CEE57 pKa = 4.16DD58 pKa = 3.73QEE60 pKa = 4.58VGEE63 pKa = 4.21SLIVPFVEE71 pKa = 4.18EE72 pKa = 4.09WLAQNAA78 pKa = 3.67

Molecular weight: 8.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3T9K8|A0A6M3T9K8_9CAUD Uncharacterized protein OS=Aminobacter phage Erebus OX=2686310 PE=4 SV=1
MM1 pKa = 7.34LRR3 pKa = 11.84YY4 pKa = 10.14DD5 pKa = 3.3IGEE8 pKa = 3.99LAVRR12 pKa = 11.84KK13 pKa = 8.02TGVGFLPQLHH23 pKa = 6.56GLPATEE29 pKa = 3.96AQYY32 pKa = 11.4LAILRR37 pKa = 11.84AMLRR41 pKa = 11.84ALNKK45 pKa = 9.91AVRR48 pKa = 11.84LDD50 pKa = 3.71VVPVAEE56 pKa = 4.47VEE58 pKa = 4.13MAAQRR63 pKa = 11.84RR64 pKa = 11.84ITRR67 pKa = 11.84DD68 pKa = 2.43VDD70 pKa = 3.13AGLFEE75 pKa = 6.24RR76 pKa = 11.84ILNLGRR82 pKa = 11.84ALGNIASSTVGNVLVLASQRR102 pKa = 11.84HH103 pKa = 4.12TAAFLRR109 pKa = 11.84TAKK112 pKa = 10.32SALGIDD118 pKa = 3.96LSAVVRR124 pKa = 11.84QDD126 pKa = 4.44DD127 pKa = 3.79LATYY131 pKa = 10.67LEE133 pKa = 4.45LAATRR138 pKa = 11.84NAGLIKK144 pKa = 10.36GLSEE148 pKa = 4.06LVVGRR153 pKa = 11.84VQQTVMTAVLNGIPAKK169 pKa = 10.2DD170 pKa = 4.1LRR172 pKa = 11.84VQLANDD178 pKa = 4.12FGFADD183 pKa = 4.11ARR185 pKa = 11.84AKK187 pKa = 10.72LIARR191 pKa = 11.84DD192 pKa = 3.59QTAKK196 pKa = 9.64LTSDD200 pKa = 4.02LNRR203 pKa = 11.84FRR205 pKa = 11.84HH206 pKa = 4.55TQAGITHH213 pKa = 5.49YY214 pKa = 10.48VWRR217 pKa = 11.84TSQDD221 pKa = 2.64EE222 pKa = 4.35RR223 pKa = 11.84VRR225 pKa = 11.84PRR227 pKa = 11.84HH228 pKa = 5.21RR229 pKa = 11.84HH230 pKa = 5.17LDD232 pKa = 3.49GLAYY236 pKa = 9.88EE237 pKa = 4.36YY238 pKa = 11.04GKK240 pKa = 8.54PTGAEE245 pKa = 3.69NGLPPGQPIQCRR257 pKa = 11.84CIAQAIVNFGDD268 pKa = 3.48TPLAITNGQRR278 pKa = 11.84YY279 pKa = 8.26IAVEE283 pKa = 3.84ATALPKK289 pKa = 10.44SPPRR293 pKa = 11.84ARR295 pKa = 11.84RR296 pKa = 11.84RR297 pKa = 11.84RR298 pKa = 11.84LRR300 pKa = 3.54

Molecular weight: 32.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

90

0

90

16371

29

794

181.9

20.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.451 ± 0.388

1.051 ± 0.131

5.907 ± 0.194

6.328 ± 0.247

3.702 ± 0.201

7.642 ± 0.241

2.016 ± 0.16

5.32 ± 0.175

5.394 ± 0.28

8.362 ± 0.243

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.95 ± 0.123

3.867 ± 0.167

4.801 ± 0.188

3.634 ± 0.265

6.09 ± 0.218

5.107 ± 0.18

6.029 ± 0.204

7.073 ± 0.194

1.594 ± 0.111

2.682 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski