Hydnomerulius pinastri MD-312

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Boletales; Paxilineae; Paxillaceae; Hydnomerulius; Hydnomerulius pinastri

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7783 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C9V409|A0A0C9V409_9AGAM Unplaced genomic scaffold scaffold_42 whole genome shotgun sequence OS=Hydnomerulius pinastri MD-312 OX=994086 GN=HYDPIDRAFT_32532 PE=4 SV=1
MM1 pKa = 7.4SRR3 pKa = 11.84NAHH6 pKa = 6.62DD7 pKa = 4.01AEE9 pKa = 4.26DD10 pKa = 3.27WRR12 pKa = 11.84NYY14 pKa = 8.21YY15 pKa = 9.8VNRR18 pKa = 11.84FVDD21 pKa = 3.7HH22 pKa = 7.24DD23 pKa = 4.06MVMHH27 pKa = 5.64YY28 pKa = 10.55HH29 pKa = 6.3SGLAVGHH36 pKa = 6.8TYY38 pKa = 7.84THH40 pKa = 7.05ISRR43 pKa = 11.84PAEE46 pKa = 3.85TSQTNSNVDD55 pKa = 3.75PGPPTALPPIPGASNSGKK73 pKa = 10.61GGDD76 pKa = 3.52WAGGTDD82 pKa = 4.72GDD84 pKa = 5.66NNDD87 pKa = 5.05DD88 pKa = 5.99DD89 pKa = 4.18NASWTKK95 pKa = 10.49SDD97 pKa = 4.35HH98 pKa = 6.25SASDD102 pKa = 3.9LDD104 pKa = 5.08DD105 pKa = 4.34GCEE108 pKa = 3.97SDD110 pKa = 5.1SDD112 pKa = 4.08SDD114 pKa = 4.98LDD116 pKa = 4.13LLVDD120 pKa = 4.03AMYY123 pKa = 10.5EE124 pKa = 4.15SEE126 pKa = 4.52VDD128 pKa = 3.56SEE130 pKa = 4.21QDD132 pKa = 2.66IGMGSGDD139 pKa = 3.84FQYY142 pKa = 11.23DD143 pKa = 3.5GYY145 pKa = 11.52EE146 pKa = 3.83FF147 pKa = 4.64

Molecular weight: 15.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C9V6Q8|A0A0C9V6Q8_9AGAM Unplaced genomic scaffold scaffold_29 whole genome shotgun sequence OS=Hydnomerulius pinastri MD-312 OX=994086 GN=HYDPIDRAFT_169751 PE=4 SV=1
MM1 pKa = 7.6SSAQGKK7 pKa = 7.86EE8 pKa = 3.5RR9 pKa = 11.84SYY11 pKa = 11.39PRR13 pKa = 11.84ALPLRR18 pKa = 11.84SRR20 pKa = 11.84RR21 pKa = 11.84KK22 pKa = 9.68KK23 pKa = 10.62SYY25 pKa = 9.72LQTRR29 pKa = 11.84SFFVVQLRR37 pKa = 11.84HH38 pKa = 5.58VSDD41 pKa = 4.24HH42 pKa = 6.28AAHH45 pKa = 5.99NHH47 pKa = 3.45MHH49 pKa = 6.92HH50 pKa = 6.51YY51 pKa = 9.22YY52 pKa = 10.99KK53 pKa = 10.68NGQEE57 pKa = 4.03SRR59 pKa = 11.84LDD61 pKa = 3.29QAIRR65 pKa = 11.84EE66 pKa = 4.3FKK68 pKa = 10.54HH69 pKa = 6.82AVDD72 pKa = 4.07RR73 pKa = 11.84CPGRR77 pKa = 11.84SAALSNLATAKK88 pKa = 10.5FISCQARR95 pKa = 11.84EE96 pKa = 3.94AHH98 pKa = 6.85LDD100 pKa = 3.29LDD102 pKa = 4.21EE103 pKa = 5.58PISLFRR109 pKa = 11.84EE110 pKa = 4.33ALDD113 pKa = 3.82LRR115 pKa = 11.84PPHH118 pKa = 7.14DD119 pKa = 4.79PDD121 pKa = 5.02HH122 pKa = 7.1PCTLINLSIALLARR136 pKa = 11.84EE137 pKa = 4.01PRR139 pKa = 11.84RR140 pKa = 11.84RR141 pKa = 11.84LMIRR145 pKa = 11.84QARR148 pKa = 3.63

Molecular weight: 17.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7781

2

7783

2994580

49

5106

384.8

42.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.53 ± 0.026

1.389 ± 0.012

5.555 ± 0.022

5.838 ± 0.025

3.729 ± 0.019

6.597 ± 0.024

2.687 ± 0.018

4.829 ± 0.019

4.341 ± 0.025

9.354 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.095 ± 0.011

3.356 ± 0.015

6.451 ± 0.034

3.77 ± 0.02

6.066 ± 0.023

8.741 ± 0.038

6.028 ± 0.019

6.423 ± 0.02

1.533 ± 0.011

2.689 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski