[Clostridium] aminophilum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Lachnoclostridium

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2701 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I0DPA6|A0A1I0DPA6_9FIRM cAMP-binding domain of CRP or a regulatory subunit of cAMP-dependent protein kinases OS=[Clostridium] aminophilum OX=1526 GN=SAMN04487771_101331 PE=4 SV=1
MM1 pKa = 7.83EE2 pKa = 5.0EE3 pKa = 4.39PKK5 pKa = 10.39PPCGLWTGIEE15 pKa = 4.26KK16 pKa = 10.24EE17 pKa = 4.42EE18 pKa = 3.93KK19 pKa = 9.91MNMNRR24 pKa = 11.84YY25 pKa = 9.82AFDD28 pKa = 3.38NTEE31 pKa = 4.1DD32 pKa = 3.9TEE34 pKa = 4.27EE35 pKa = 4.15MSDD38 pKa = 4.17LEE40 pKa = 4.2QSLEE44 pKa = 3.97PVIDD48 pKa = 4.01PGVNPAYY55 pKa = 10.23YY56 pKa = 9.91GQSEE60 pKa = 5.2EE61 pKa = 4.49DD62 pKa = 3.42TAPDD66 pKa = 4.06PEE68 pKa = 4.43EE69 pKa = 5.06LLDD72 pKa = 5.46GYY74 pKa = 10.59IQVLKK79 pKa = 10.73ALNYY83 pKa = 10.0DD84 pKa = 3.87NEE86 pKa = 4.59NIAYY90 pKa = 9.67AAGTTLEE97 pKa = 4.17RR98 pKa = 11.84VLEE101 pKa = 4.29VVGEE105 pKa = 4.2DD106 pKa = 3.21

Molecular weight: 11.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I0GNX9|A0A1I0GNX9_9FIRM Hydroxymethylglutaryl-CoA lyase OS=[Clostridium] aminophilum OX=1526 GN=SAMN02910262_00995 PE=3 SV=1
MM1 pKa = 7.61SKK3 pKa = 7.84MTFQPKK9 pKa = 8.96KK10 pKa = 7.54RR11 pKa = 11.84QRR13 pKa = 11.84SRR15 pKa = 11.84VHH17 pKa = 6.33GFRR20 pKa = 11.84ARR22 pKa = 11.84MKK24 pKa = 8.43TANGRR29 pKa = 11.84KK30 pKa = 8.89VLAARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.27GRR40 pKa = 11.84ASLTVV45 pKa = 3.04

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2701

0

2701

915300

39

5356

338.9

37.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.443 ± 0.055

1.4 ± 0.019

6.005 ± 0.038

7.573 ± 0.058

4.014 ± 0.03

7.558 ± 0.043

1.782 ± 0.02

6.895 ± 0.042

5.986 ± 0.035

8.596 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.2 ± 0.023

4.003 ± 0.027

3.573 ± 0.029

2.828 ± 0.025

5.378 ± 0.046

5.913 ± 0.044

5.427 ± 0.039

6.814 ± 0.036

0.879 ± 0.013

3.734 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski