Mesta yellow vein mosaic virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A3FF33|A3FF33_9GEMI Capsid protein OS=Mesta yellow vein mosaic virus OX=360579 PE=3 SV=1
MM1 pKa = 8.06WDD3 pKa = 3.35PLLNEE8 pKa = 4.38FPEE11 pKa = 4.68TVHH14 pKa = 7.0GFRR17 pKa = 11.84CMLAIKK23 pKa = 9.87YY24 pKa = 7.48LQQLSEE30 pKa = 4.6EE31 pKa = 4.3YY32 pKa = 10.92SPDD35 pKa = 3.21TVGYY39 pKa = 10.56DD40 pKa = 4.37LIRR43 pKa = 11.84DD44 pKa = 4.65LISILRR50 pKa = 11.84SRR52 pKa = 11.84NYY54 pKa = 9.91VEE56 pKa = 4.26ASCRR60 pKa = 11.84YY61 pKa = 7.53RR62 pKa = 11.84HH63 pKa = 6.36FYY65 pKa = 10.07PRR67 pKa = 11.84VEE69 pKa = 4.35GASSTEE75 pKa = 3.75LRR77 pKa = 11.84QPLCNPCSCPHH88 pKa = 6.45CPRR91 pKa = 11.84HH92 pKa = 5.27KK93 pKa = 10.0VSYY96 pKa = 10.19VGEE99 pKa = 4.04QAHH102 pKa = 5.97VSEE105 pKa = 4.79AQNVQDD111 pKa = 3.65VQKK114 pKa = 10.62PP115 pKa = 3.38

Molecular weight: 13.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A3FF34|A3FF34_9GEMI Replication enhancer OS=Mesta yellow vein mosaic virus OX=360579 PE=3 SV=1
MM1 pKa = 7.71SKK3 pKa = 10.35RR4 pKa = 11.84PADD7 pKa = 3.52IVISTPASKK16 pKa = 10.29VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.0SPYY27 pKa = 9.21ATRR30 pKa = 11.84AVAPTVRR37 pKa = 11.84VTKK40 pKa = 10.7SRR42 pKa = 11.84MWANRR47 pKa = 11.84PMYY50 pKa = 9.66RR51 pKa = 11.84KK52 pKa = 8.75PRR54 pKa = 11.84MYY56 pKa = 10.82RR57 pKa = 11.84MYY59 pKa = 10.57RR60 pKa = 11.84SPDD63 pKa = 3.29VPKK66 pKa = 10.81GCEE69 pKa = 4.39GPCKK73 pKa = 10.37VQSFDD78 pKa = 3.26AKK80 pKa = 11.06NDD82 pKa = 3.27IGHH85 pKa = 6.11MGKK88 pKa = 10.22VICLSDD94 pKa = 3.33VTRR97 pKa = 11.84GIGLTHH103 pKa = 6.67RR104 pKa = 11.84VGKK107 pKa = 9.78RR108 pKa = 11.84FCVKK112 pKa = 10.05SLYY115 pKa = 10.31FVGKK119 pKa = 9.14IWMDD123 pKa = 3.43EE124 pKa = 4.06NIKK127 pKa = 9.32TKK129 pKa = 10.61NHH131 pKa = 6.02TNTVMFWIVRR141 pKa = 11.84DD142 pKa = 3.87RR143 pKa = 11.84RR144 pKa = 11.84PSGTPNDD151 pKa = 3.78FQQVFNVYY159 pKa = 10.67DD160 pKa = 4.09NEE162 pKa = 4.37PSTATVKK169 pKa = 10.59NDD171 pKa = 3.0QRR173 pKa = 11.84DD174 pKa = 3.36RR175 pKa = 11.84FQVLRR180 pKa = 11.84RR181 pKa = 11.84FQATVTGGQYY191 pKa = 10.18AAKK194 pKa = 8.87EE195 pKa = 3.58QAIIRR200 pKa = 11.84KK201 pKa = 8.88FYY203 pKa = 10.48RR204 pKa = 11.84VNNYY208 pKa = 7.79VVYY211 pKa = 10.32NHH213 pKa = 6.0QEE215 pKa = 3.43AGKK218 pKa = 9.63YY219 pKa = 8.2EE220 pKa = 4.08NHH222 pKa = 5.93TVLMPLTLCMLLL234 pKa = 3.92

Molecular weight: 27.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1096

100

363

182.7

20.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.018 ± 0.718

2.281 ± 0.565

4.562 ± 0.345

5.201 ± 0.947

4.288 ± 0.37

5.292 ± 0.4

3.011 ± 0.457

4.745 ± 0.696

5.383 ± 0.967

7.755 ± 0.986

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.099 ± 0.541

5.566 ± 0.332

6.113 ± 0.378

5.474 ± 0.567

7.026 ± 1.163

9.215 ± 1.677

5.748 ± 1.179

5.748 ± 1.08

1.46 ± 0.168

4.015 ± 0.629
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski