Candidatus Gracilibacteria bacterium GN02-873

Taxonomy: cellular organisms; Bacteria; Bacteria incertae sedis; Bacteria candidate phyla; Patescibacteria group; Candidatus Gracilibacteria; unclassified Candidatus Gracilibacteria

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 908 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A328EBK2|A0A328EBK2_9BACT Peptidase C51 domain-containing protein OS=Candidatus Gracilibacteria bacterium GN02-873 OX=1914866 GN=BLM37_04640 PE=4 SV=1
MM1 pKa = 7.99DD2 pKa = 4.74FSFIPAVSAATTPILDD18 pKa = 3.38ASVNYY23 pKa = 7.06TTSDD27 pKa = 3.25MLRR30 pKa = 11.84IAISLIVLVAMVCAVFFIVYY50 pKa = 9.5GGLMLVLSGGNDD62 pKa = 3.55DD63 pKa = 4.69KK64 pKa = 11.75VSSALGSIRR73 pKa = 11.84YY74 pKa = 8.74AVIGLVVIVVAIFVTPHH91 pKa = 6.05ISRR94 pKa = 11.84MLGLGSQDD102 pKa = 3.41YY103 pKa = 10.76LSPQSITYY111 pKa = 7.85TIQTLSEE118 pKa = 4.52RR119 pKa = 11.84IFGSTSGAFTPSSTSAIDD137 pKa = 3.71FDD139 pKa = 5.25DD140 pKa = 4.01FF141 pKa = 5.42

Molecular weight: 14.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A328EEN6|A0A328EEN6_9BACT Ribosomal RNA small subunit methyltransferase H OS=Candidatus Gracilibacteria bacterium GN02-873 OX=1914866 GN=rsmH PE=3 SV=1
MM1 pKa = 7.23AHH3 pKa = 6.77KK4 pKa = 10.4KK5 pKa = 10.93AMGSTANGRR14 pKa = 11.84DD15 pKa = 3.52SVAKK19 pKa = 9.97RR20 pKa = 11.84LGVKK24 pKa = 9.88IYY26 pKa = 10.66GGQPALAGNIIVRR39 pKa = 11.84QKK41 pKa = 11.64GNTFWAGQGVSQGNDD56 pKa = 3.06YY57 pKa = 9.34TLFATRR63 pKa = 11.84DD64 pKa = 3.56GVVQFQEE71 pKa = 3.77RR72 pKa = 11.84RR73 pKa = 11.84RR74 pKa = 11.84TRR76 pKa = 11.84FDD78 pKa = 2.78GRR80 pKa = 11.84VYY82 pKa = 10.46RR83 pKa = 11.84DD84 pKa = 2.95IYY86 pKa = 10.71VSVVV90 pKa = 2.65

Molecular weight: 9.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

908

0

908

278524

59

4110

306.7

34.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.355 ± 0.089

0.749 ± 0.024

5.201 ± 0.059

7.475 ± 0.102

5.815 ± 0.089

6.208 ± 0.075

1.787 ± 0.041

8.809 ± 0.096

8.022 ± 0.091

8.638 ± 0.08

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.38 ± 0.043

5.906 ± 0.088

3.358 ± 0.055

3.557 ± 0.053

4.0 ± 0.046

6.434 ± 0.077

5.6 ± 0.089

5.403 ± 0.069

0.841 ± 0.024

3.463 ± 0.058
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski