Streptomyces phage Yaboi

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Karimacvirus; Streptomyces virus Yaboi

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 215 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385UIB5|A0A385UIB5_9CAUD Deoxycytidylate deaminase OS=Streptomyces phage Yaboi OX=2301621 GN=233 PE=4 SV=1
MM1 pKa = 7.76SYY3 pKa = 10.54QLQVLADD10 pKa = 3.8SPFSYY15 pKa = 9.85WKK17 pKa = 10.4LDD19 pKa = 3.39GSGPVYY25 pKa = 10.65NDD27 pKa = 2.88SAGSLRR33 pKa = 11.84QADD36 pKa = 4.11LTSTAVIHH44 pKa = 6.43PALILGSGNALVLQNTNQLQMDD66 pKa = 4.02DD67 pKa = 3.63PVFNKK72 pKa = 10.33GYY74 pKa = 10.08EE75 pKa = 4.01SRR77 pKa = 11.84QFSLEE82 pKa = 3.7AWVKK86 pKa = 8.87PLSITGEE93 pKa = 4.05VSIMSHH99 pKa = 5.47NEE101 pKa = 3.62NYY103 pKa = 10.35DD104 pKa = 3.35GLTITPNRR112 pKa = 11.84IYY114 pKa = 10.76FRR116 pKa = 11.84TKK118 pKa = 10.47YY119 pKa = 8.76LTAPASEE126 pKa = 3.96ISYY129 pKa = 10.46KK130 pKa = 10.56YY131 pKa = 9.06DD132 pKa = 3.1TAKK135 pKa = 10.56AFHH138 pKa = 6.23VVGVHH143 pKa = 5.22TNGKK147 pKa = 8.05NALYY151 pKa = 10.85VDD153 pKa = 4.22GQLVAEE159 pKa = 4.57IDD161 pKa = 3.52ITDD164 pKa = 3.79EE165 pKa = 4.18QSADD169 pKa = 2.98AYY171 pKa = 11.21NFVSSDD177 pKa = 4.17LIAGQSATSSAIALDD192 pKa = 3.69APAIYY197 pKa = 9.9AAPLSAEE204 pKa = 4.46TIKK207 pKa = 10.61KK208 pKa = 9.81HH209 pKa = 5.2YY210 pKa = 10.31DD211 pKa = 3.12YY212 pKa = 11.25GIDD215 pKa = 3.73VEE217 pKa = 4.48SSEE220 pKa = 5.77AIAGYY225 pKa = 10.78NDD227 pKa = 3.2ASHH230 pKa = 6.97WNFADD235 pKa = 3.4SARR238 pKa = 11.84NIAVSKK244 pKa = 10.59LWDD247 pKa = 3.82DD248 pKa = 4.39EE249 pKa = 4.18EE250 pKa = 5.09WIDD253 pKa = 5.69GIMTDD258 pKa = 3.98VVIANDD264 pKa = 4.23IIVPSYY270 pKa = 10.63VQTEE274 pKa = 4.3TEE276 pKa = 4.07EE277 pKa = 4.48VIDD280 pKa = 3.99GLIVPVYY287 pKa = 10.06TNTSLPGVWTGSMEE301 pKa = 3.98IGEE304 pKa = 4.94SISNITDD311 pKa = 2.98AVIRR315 pKa = 11.84YY316 pKa = 9.0RR317 pKa = 11.84GEE319 pKa = 3.81GSFTIEE325 pKa = 4.16CSIDD329 pKa = 4.96NGDD332 pKa = 3.06TWQSVDD338 pKa = 3.54SAPGFSLTTEE348 pKa = 3.91DD349 pKa = 4.18SILVRR354 pKa = 11.84VTFAGGIVDD363 pKa = 3.98DD364 pKa = 4.16QSYY367 pKa = 10.9VDD369 pKa = 4.38WIQIVAYY376 pKa = 9.33SDD378 pKa = 3.43KK379 pKa = 10.75VFNGTRR385 pKa = 11.84SDD387 pKa = 3.58RR388 pKa = 11.84QATLTGTAVMARR400 pKa = 11.84EE401 pKa = 3.91FFEE404 pKa = 5.04PIEE407 pKa = 4.06YY408 pKa = 10.78ADD410 pKa = 4.32DD411 pKa = 3.85NGIKK415 pKa = 10.06ILTGDD420 pKa = 3.15ISVGVDD426 pKa = 2.87SSYY429 pKa = 11.59DD430 pKa = 3.59GEE432 pKa = 4.39EE433 pKa = 3.84EE434 pKa = 5.31AGDD437 pKa = 4.0LNINGIDD444 pKa = 3.24IWIKK448 pKa = 9.89PVAGNIITAGSASISRR464 pKa = 11.84VGNTIVFSGFSDD476 pKa = 3.79VVVNGVPVTSGTTVFTTDD494 pKa = 1.94SWYY497 pKa = 10.62HH498 pKa = 4.79IAGVFTTPGNYY509 pKa = 10.25ALTMGVANARR519 pKa = 11.84IAQASAIYY527 pKa = 10.65GDD529 pKa = 3.94IDD531 pKa = 4.94LDD533 pKa = 5.07GIQQMYY539 pKa = 9.75SAYY542 pKa = 10.55LGLPGLTVDD551 pKa = 4.56DD552 pKa = 4.2VTPIGIDD559 pKa = 3.27TANPEE564 pKa = 4.32ALLYY568 pKa = 10.09AHH570 pKa = 6.76VWSISPAGG578 pKa = 3.42

Molecular weight: 62.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385UH49|A0A385UH49_9CAUD Uncharacterized protein OS=Streptomyces phage Yaboi OX=2301621 GN=135 PE=4 SV=1
MM1 pKa = 7.34TVTLLSRR8 pKa = 11.84RR9 pKa = 11.84LSRR12 pKa = 11.84CHH14 pKa = 5.7TLRR17 pKa = 11.84NVTVSLCHH25 pKa = 6.06ICHH28 pKa = 6.79FLICHH33 pKa = 6.37APGGGRR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84GSAFVVLYY49 pKa = 9.78ILRR52 pKa = 11.84RR53 pKa = 11.84SKK55 pKa = 11.09GRR57 pKa = 11.84GDD59 pKa = 3.2LTEE62 pKa = 3.64QEE64 pKa = 4.52RR65 pKa = 11.84PVKK68 pKa = 10.57VNYY71 pKa = 8.23TGKK74 pKa = 10.33RR75 pKa = 11.84KK76 pKa = 9.71EE77 pKa = 4.04KK78 pKa = 10.28EE79 pKa = 3.73

Molecular weight: 9.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

215

0

215

33893

20

2093

157.6

17.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.258 ± 0.366

1.083 ± 0.134

6.379 ± 0.201

7.173 ± 0.364

3.809 ± 0.123

7.373 ± 0.175

1.729 ± 0.121

5.497 ± 0.149

6.497 ± 0.342

7.305 ± 0.211

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.915 ± 0.137

4.682 ± 0.158

3.65 ± 0.166

3.204 ± 0.187

5.73 ± 0.196

5.957 ± 0.294

6.01 ± 0.394

7.099 ± 0.228

1.947 ± 0.095

3.703 ± 0.167
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski