Kribbella sp. VKM Ac-2500

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Kribbellaceae; Kribbella; unclassified Kribbella

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8969 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R2C8C5|A0A4R2C8C5_9ACTN Uncharacterized protein OS=Kribbella sp. VKM Ac-2500 OX=2512214 GN=EV642_11390 PE=4 SV=1
MM1 pKa = 7.61LLLTTTVPAAAVIDD15 pKa = 4.12GTTDD19 pKa = 3.29TTNSFPNVGGLQLQDD34 pKa = 3.04EE35 pKa = 5.1GEE37 pKa = 4.1WFDD40 pKa = 3.91FCTGTLVAPDD50 pKa = 3.78VVLTAAHH57 pKa = 6.1CTDD60 pKa = 4.87FFTGDD65 pKa = 3.31VGDD68 pKa = 4.88PDD70 pKa = 5.19ALGPDD75 pKa = 3.85DD76 pKa = 3.79WRR78 pKa = 11.84VSFDD82 pKa = 4.08ADD84 pKa = 3.49PDD86 pKa = 4.03EE87 pKa = 4.49NSTYY91 pKa = 10.81YY92 pKa = 10.9GADD95 pKa = 3.15HH96 pKa = 6.68FVVHH100 pKa = 6.88PDD102 pKa = 2.68WLANQVGPGGGNSKK116 pKa = 9.92MSFLKK121 pKa = 10.86DD122 pKa = 3.37GLEE125 pKa = 4.65DD126 pKa = 3.23IALVFLTEE134 pKa = 4.32DD135 pKa = 3.56VANVTPAPVADD146 pKa = 3.95AGYY149 pKa = 11.02LDD151 pKa = 5.1GLDD154 pKa = 3.64LTSEE158 pKa = 4.31TFTVVGYY165 pKa = 8.16GTDD168 pKa = 3.34EE169 pKa = 4.38YY170 pKa = 10.61VTGSAASPMAITVYY184 pKa = 10.74DD185 pKa = 3.79GARR188 pKa = 11.84SYY190 pKa = 11.89KK191 pKa = 10.35DD192 pKa = 2.93VTVITKK198 pKa = 9.99QDD200 pKa = 3.64LFPDD204 pKa = 3.98RR205 pKa = 11.84FVKK208 pKa = 8.85ITKK211 pKa = 8.97SVCFGDD217 pKa = 3.12SGGPLFHH224 pKa = 7.35EE225 pKa = 5.17GTLVALNTWTFSYY238 pKa = 10.74RR239 pKa = 11.84CDD241 pKa = 3.92GPNLEE246 pKa = 4.52YY247 pKa = 10.57RR248 pKa = 11.84VDD250 pKa = 3.71SAPAQSFLDD259 pKa = 3.78ANLL262 pKa = 3.46

Molecular weight: 28.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R2CC41|A0A4R2CC41_9ACTN Release factor glutamine methyltransferase OS=Kribbella sp. VKM Ac-2500 OX=2512214 GN=EV642_11447 PE=4 SV=1
MM1 pKa = 7.9DD2 pKa = 5.63RR3 pKa = 11.84GTGRR7 pKa = 11.84VSRR10 pKa = 11.84WIRR13 pKa = 11.84GLSGRR18 pKa = 11.84KK19 pKa = 9.23GSNLPLSSLGPWTHH33 pKa = 5.53VSSGRR38 pKa = 11.84RR39 pKa = 11.84GSNTGLVRR47 pKa = 11.84ALRR50 pKa = 11.84MVRR53 pKa = 11.84RR54 pKa = 11.84RR55 pKa = 11.84GLSIRR60 pKa = 11.84GLSGRR65 pKa = 11.84RR66 pKa = 11.84GGRR69 pKa = 11.84KK70 pKa = 9.02GRR72 pKa = 11.84GRR74 pKa = 11.84LGPVVLRR81 pKa = 11.84HH82 pKa = 5.63IRR84 pKa = 11.84RR85 pKa = 11.84LL86 pKa = 3.43

Molecular weight: 9.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8969

0

8969

2723140

25

3447

303.6

32.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.768 ± 0.037

0.711 ± 0.008

6.088 ± 0.025

5.564 ± 0.026

2.907 ± 0.013

8.892 ± 0.025

2.106 ± 0.013

3.881 ± 0.018

2.296 ± 0.02

10.544 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.752 ± 0.009

2.067 ± 0.014

5.763 ± 0.022

3.114 ± 0.013

7.43 ± 0.026

5.487 ± 0.018

6.172 ± 0.022

8.686 ± 0.024

1.622 ± 0.011

2.148 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski