Sphingomonas phage vB_StuS_MMDA13

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 89 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G3PLV6|A0A7G3PLV6_9CAUD Uncharacterized protein OS=Sphingomonas phage vB_StuS_MMDA13 OX=2686378 GN=MMDA13_gp59 PE=4 SV=1
MM1 pKa = 7.27TSRR4 pKa = 11.84RR5 pKa = 11.84PLVVIGGRR13 pKa = 11.84IQEE16 pKa = 4.56LPAGDD21 pKa = 4.27DD22 pKa = 3.32VAAVVNDD29 pKa = 3.8PEE31 pKa = 4.31FVYY34 pKa = 9.53DD35 pKa = 4.06TVADD39 pKa = 3.7ILQQGTNITLTFNDD53 pKa = 3.95TTNKK57 pKa = 8.15ITIACVVDD65 pKa = 3.39AEE67 pKa = 4.62YY68 pKa = 10.55IRR70 pKa = 11.84DD71 pKa = 3.92TIGAALVQGTGTILSVNDD89 pKa = 3.57SLNTITYY96 pKa = 9.77SVDD99 pKa = 3.01PEE101 pKa = 4.17YY102 pKa = 11.35VRR104 pKa = 11.84DD105 pKa = 4.21VIATALSASNGVALTIDD122 pKa = 3.75DD123 pKa = 5.17AGDD126 pKa = 3.83SMSLQADD133 pKa = 3.52PEE135 pKa = 4.31YY136 pKa = 10.92FRR138 pKa = 11.84DD139 pKa = 3.79LMGMTLVQGPGVVISVNDD157 pKa = 3.94AGNTITISADD167 pKa = 2.86GSTTIPFIYY176 pKa = 10.06VPAVFYY182 pKa = 10.6PGTLKK187 pKa = 9.74TANIIMLRR195 pKa = 11.84HH196 pKa = 6.56AIVSDD201 pKa = 3.52CTLPANCAGSAGGGEE216 pKa = 4.55ADD218 pKa = 3.4ATSTSVYY225 pKa = 10.19KK226 pKa = 10.28IQKK229 pKa = 7.89STAALPDD236 pKa = 3.49TFTDD240 pKa = 3.36IGAITYY246 pKa = 10.55SSGTHH251 pKa = 5.83IPTFSTAGGVDD262 pKa = 3.03INFAVGDD269 pKa = 3.9TLRR272 pKa = 11.84VVSPDD277 pKa = 3.43VPDD280 pKa = 3.64TTLSDD285 pKa = 3.71TNFTILMNRR294 pKa = 11.84SSS296 pKa = 3.33

Molecular weight: 30.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G3PHP7|A0A7G3PHP7_9CAUD Putative transcriptional regulator OS=Sphingomonas phage vB_StuS_MMDA13 OX=2686378 GN=MMDA13_gp57 PE=4 SV=1
MM1 pKa = 7.64LYY3 pKa = 10.45SEE5 pKa = 5.06AAYY8 pKa = 10.24FDD10 pKa = 4.76RR11 pKa = 11.84NHH13 pKa = 6.92CAHH16 pKa = 6.87CAARR20 pKa = 11.84DD21 pKa = 3.36EE22 pKa = 4.38HH23 pKa = 6.6MRR25 pKa = 11.84LQRR28 pKa = 11.84KK29 pKa = 9.35RR30 pKa = 11.84IEE32 pKa = 4.74AIRR35 pKa = 11.84SLDD38 pKa = 3.1RR39 pKa = 11.84RR40 pKa = 11.84TSRR43 pKa = 11.84RR44 pKa = 3.34

Molecular weight: 5.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

89

0

89

19973

42

956

224.4

24.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.674 ± 0.603

1.137 ± 0.128

6.103 ± 0.161

6.118 ± 0.286

3.815 ± 0.173

7.796 ± 0.282

1.933 ± 0.173

4.852 ± 0.2

5.032 ± 0.287

7.781 ± 0.245

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.729 ± 0.106

4.486 ± 0.201

5.172 ± 0.227

4.045 ± 0.238

5.688 ± 0.264

5.257 ± 0.205

6.058 ± 0.294

6.659 ± 0.226

1.637 ± 0.122

3.029 ± 0.155
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski