Amino acid dipepetide frequency for Arthrobacter phage Maureen

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
23.366AlaAla: 23.366 ± 2.084
0.976AlaCys: 0.976 ± 0.253
10.881AlaAsp: 10.881 ± 1.197
8.44AlaGlu: 8.44 ± 0.827
3.139AlaPhe: 3.139 ± 0.423
11.09AlaGly: 11.09 ± 0.888
2.023AlaHis: 2.023 ± 0.377
5.65AlaIle: 5.65 ± 0.892
6.068AlaLys: 6.068 ± 0.717
12.694AlaLeu: 12.694 ± 1.106
3.069AlaMet: 3.069 ± 0.539
2.092AlaAsn: 2.092 ± 0.552
8.509AlaPro: 8.509 ± 1.028
5.301AlaGln: 5.301 ± 0.839
11.02AlaArg: 11.02 ± 1.293
6.905AlaSer: 6.905 ± 0.77
8.23AlaThr: 8.23 ± 0.747
11.23AlaVal: 11.23 ± 0.971
2.371AlaTrp: 2.371 ± 0.329
3.069AlaTyr: 3.069 ± 0.323
0.0AlaXaa: 0.0 ± 0.0
Cys
0.697CysAla: 0.697 ± 0.213
0.0CysCys: 0.0 ± 0.0
0.279CysAsp: 0.279 ± 0.14
0.139CysGlu: 0.139 ± 0.094
0.279CysPhe: 0.279 ± 0.121
0.837CysGly: 0.837 ± 0.272
0.139CysHis: 0.139 ± 0.104
0.0CysIle: 0.0 ± 0.0
0.279CysLys: 0.279 ± 0.156
0.628CysLeu: 0.628 ± 0.22
0.0CysMet: 0.0 ± 0.0
0.07CysAsn: 0.07 ± 0.069
0.697CysPro: 0.697 ± 0.241
0.07CysGln: 0.07 ± 0.1
0.837CysArg: 0.837 ± 0.257
0.07CysSer: 0.07 ± 0.069
0.07CysThr: 0.07 ± 0.061
0.418CysVal: 0.418 ± 0.148
0.209CysTrp: 0.209 ± 0.137
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
10.951AspAla: 10.951 ± 1.267
0.488AspCys: 0.488 ± 0.176
5.859AspAsp: 5.859 ± 0.7
3.208AspGlu: 3.208 ± 0.518
2.162AspPhe: 2.162 ± 0.377
7.463AspGly: 7.463 ± 0.863
0.907AspHis: 0.907 ± 0.253
1.953AspIle: 1.953 ± 0.297
1.813AspLys: 1.813 ± 0.303
8.37AspLeu: 8.37 ± 0.945
0.628AspMet: 0.628 ± 0.166
0.976AspAsn: 0.976 ± 0.224
5.44AspPro: 5.44 ± 0.764
2.023AspGln: 2.023 ± 0.345
5.371AspArg: 5.371 ± 0.722
2.371AspSer: 2.371 ± 0.464
4.115AspThr: 4.115 ± 0.464
6.696AspVal: 6.696 ± 0.486
0.976AspTrp: 0.976 ± 0.27
2.441AspTyr: 2.441 ± 0.505
0.0AspXaa: 0.0 ± 0.0
Glu
9.346GluAla: 9.346 ± 0.775
0.279GluCys: 0.279 ± 0.129
2.302GluAsp: 2.302 ± 0.425
2.72GluGlu: 2.72 ± 0.494
0.837GluPhe: 0.837 ± 0.21
4.603GluGly: 4.603 ± 0.625
1.046GluHis: 1.046 ± 0.251
2.72GluIle: 2.72 ± 0.432
1.465GluLys: 1.465 ± 0.365
3.139GluLeu: 3.139 ± 0.582
0.488GluMet: 0.488 ± 0.197
1.186GluAsn: 1.186 ± 0.317
1.395GluPro: 1.395 ± 0.303
1.186GluGln: 1.186 ± 0.328
3.906GluArg: 3.906 ± 0.667
3.418GluSer: 3.418 ± 0.51
3.348GluThr: 3.348 ± 0.472
4.255GluVal: 4.255 ± 0.591
1.395GluTrp: 1.395 ± 0.32
1.465GluTyr: 1.465 ± 0.301
0.0GluXaa: 0.0 ± 0.0
Phe
3.697PheAla: 3.697 ± 0.438
0.139PheCys: 0.139 ± 0.103
2.302PheAsp: 2.302 ± 0.363
1.116PheGlu: 1.116 ± 0.272
0.697PhePhe: 0.697 ± 0.262
3.557PheGly: 3.557 ± 0.51
0.697PheHis: 0.697 ± 0.198
0.697PheIle: 0.697 ± 0.249
1.186PheLys: 1.186 ± 0.462
2.72PheLeu: 2.72 ± 0.576
0.209PheMet: 0.209 ± 0.112
0.279PheAsn: 0.279 ± 0.118
1.395PhePro: 1.395 ± 0.336
1.186PheGln: 1.186 ± 0.234
1.534PheArg: 1.534 ± 0.41
1.116PheSer: 1.116 ± 0.291
2.371PheThr: 2.371 ± 0.422
2.092PheVal: 2.092 ± 0.375
0.488PheTrp: 0.488 ± 0.195
0.837PheTyr: 0.837 ± 0.23
0.0PheXaa: 0.0 ± 0.0
Gly
10.532GlyAla: 10.532 ± 1.085
0.488GlyCys: 0.488 ± 0.167
6.905GlyAsp: 6.905 ± 0.77
4.115GlyGlu: 4.115 ± 0.513
3.069GlyPhe: 3.069 ± 0.579
8.37GlyGly: 8.37 ± 1.19
1.325GlyHis: 1.325 ± 0.282
3.697GlyIle: 3.697 ± 0.824
3.418GlyLys: 3.418 ± 0.491
7.951GlyLeu: 7.951 ± 0.883
1.325GlyMet: 1.325 ± 0.322
1.883GlyAsn: 1.883 ± 0.523
4.115GlyPro: 4.115 ± 0.566
1.813GlyGln: 1.813 ± 0.352
6.696GlyArg: 6.696 ± 0.815
4.952GlySer: 4.952 ± 0.936
4.673GlyThr: 4.673 ± 0.894
6.905GlyVal: 6.905 ± 0.857
1.604GlyTrp: 1.604 ± 0.392
2.999GlyTyr: 2.999 ± 0.474
0.0GlyXaa: 0.0 ± 0.0
His
1.813HisAla: 1.813 ± 0.437
0.139HisCys: 0.139 ± 0.086
1.186HisAsp: 1.186 ± 0.37
0.558HisGlu: 0.558 ± 0.202
0.697HisPhe: 0.697 ± 0.247
1.604HisGly: 1.604 ± 0.397
0.418HisHis: 0.418 ± 0.178
0.279HisIle: 0.279 ± 0.131
0.628HisLys: 0.628 ± 0.253
1.604HisLeu: 1.604 ± 0.373
0.279HisMet: 0.279 ± 0.152
0.628HisAsn: 0.628 ± 0.271
1.325HisPro: 1.325 ± 0.284
0.209HisGln: 0.209 ± 0.114
1.186HisArg: 1.186 ± 0.336
0.349HisSer: 0.349 ± 0.149
0.837HisThr: 0.837 ± 0.212
0.767HisVal: 0.767 ± 0.238
0.349HisTrp: 0.349 ± 0.142
0.418HisTyr: 0.418 ± 0.196
0.0HisXaa: 0.0 ± 0.0
Ile
4.394IleAla: 4.394 ± 0.501
0.07IleCys: 0.07 ± 0.071
3.348IleAsp: 3.348 ± 0.499
1.744IleGlu: 1.744 ± 0.43
0.628IlePhe: 0.628 ± 0.198
2.581IleGly: 2.581 ± 0.616
0.697IleHis: 0.697 ± 0.157
0.976IleIle: 0.976 ± 0.346
0.976IleLys: 0.976 ± 0.287
3.627IleLeu: 3.627 ± 0.571
0.488IleMet: 0.488 ± 0.211
0.558IleAsn: 0.558 ± 0.289
1.953IlePro: 1.953 ± 0.324
2.65IleGln: 2.65 ± 0.558
3.557IleArg: 3.557 ± 0.501
1.534IleSer: 1.534 ± 0.46
2.999IleThr: 2.999 ± 0.431
2.232IleVal: 2.232 ± 0.374
0.697IleTrp: 0.697 ± 0.257
0.628IleTyr: 0.628 ± 0.192
0.0IleXaa: 0.0 ± 0.0
Lys
4.743LysAla: 4.743 ± 0.697
0.349LysCys: 0.349 ± 0.144
1.744LysAsp: 1.744 ± 0.388
1.465LysGlu: 1.465 ± 0.323
1.186LysPhe: 1.186 ± 0.25
2.929LysGly: 2.929 ± 0.513
0.976LysHis: 0.976 ± 0.251
1.813LysIle: 1.813 ± 0.327
1.395LysLys: 1.395 ± 0.296
2.092LysLeu: 2.092 ± 0.379
0.767LysMet: 0.767 ± 0.228
0.976LysAsn: 0.976 ± 0.276
1.604LysPro: 1.604 ± 0.389
0.488LysGln: 0.488 ± 0.19
2.79LysArg: 2.79 ± 0.453
1.953LysSer: 1.953 ± 0.342
1.325LysThr: 1.325 ± 0.323
3.418LysVal: 3.418 ± 0.595
0.558LysTrp: 0.558 ± 0.18
0.837LysTyr: 0.837 ± 0.195
0.0LysXaa: 0.0 ± 0.0
Leu
14.229LeuAla: 14.229 ± 1.047
0.209LeuCys: 0.209 ± 0.141
7.951LeuAsp: 7.951 ± 0.76
3.557LeuGlu: 3.557 ± 0.468
1.883LeuPhe: 1.883 ± 0.377
7.463LeuGly: 7.463 ± 1.121
1.744LeuHis: 1.744 ± 0.389
3.348LeuIle: 3.348 ± 0.44
2.162LeuLys: 2.162 ± 0.306
5.789LeuLeu: 5.789 ± 0.767
1.813LeuMet: 1.813 ± 0.396
1.883LeuAsn: 1.883 ± 0.329
4.185LeuPro: 4.185 ± 0.44
1.813LeuGln: 1.813 ± 0.377
6.696LeuArg: 6.696 ± 0.815
5.092LeuSer: 5.092 ± 0.84
5.998LeuThr: 5.998 ± 0.648
6.696LeuVal: 6.696 ± 0.699
1.604LeuTrp: 1.604 ± 0.342
1.674LeuTyr: 1.674 ± 0.285
0.0LeuXaa: 0.0 ± 0.0
Met
2.929MetAla: 2.929 ± 0.487
0.07MetCys: 0.07 ± 0.072
0.697MetAsp: 0.697 ± 0.239
0.418MetGlu: 0.418 ± 0.145
0.279MetPhe: 0.279 ± 0.129
1.046MetGly: 1.046 ± 0.315
0.488MetHis: 0.488 ± 0.2
0.976MetIle: 0.976 ± 0.283
0.767MetLys: 0.767 ± 0.324
1.116MetLeu: 1.116 ± 0.289
0.209MetMet: 0.209 ± 0.122
0.209MetAsn: 0.209 ± 0.131
1.255MetPro: 1.255 ± 0.326
0.07MetGln: 0.07 ± 0.078
1.046MetArg: 1.046 ± 0.242
1.604MetSer: 1.604 ± 0.333
2.023MetThr: 2.023 ± 0.38
1.046MetVal: 1.046 ± 0.308
0.0MetTrp: 0.0 ± 0.0
0.07MetTyr: 0.07 ± 0.078
0.0MetXaa: 0.0 ± 0.0
Asn
1.674AsnAla: 1.674 ± 0.416
0.0AsnCys: 0.0 ± 0.0
0.837AsnAsp: 0.837 ± 0.245
0.697AsnGlu: 0.697 ± 0.232
0.976AsnPhe: 0.976 ± 0.276
2.581AsnGly: 2.581 ± 0.553
0.209AsnHis: 0.209 ± 0.115
0.349AsnIle: 0.349 ± 0.167
0.628AsnLys: 0.628 ± 0.158
1.534AsnLeu: 1.534 ± 0.478
0.349AsnMet: 0.349 ± 0.136
0.488AsnAsn: 0.488 ± 0.204
1.674AsnPro: 1.674 ± 0.405
0.488AsnGln: 0.488 ± 0.2
1.046AsnArg: 1.046 ± 0.314
0.767AsnSer: 0.767 ± 0.326
1.325AsnThr: 1.325 ± 0.404
1.604AsnVal: 1.604 ± 0.293
0.628AsnTrp: 0.628 ± 0.178
0.628AsnTyr: 0.628 ± 0.189
0.0AsnXaa: 0.0 ± 0.0
Pro
8.579ProAla: 8.579 ± 1.01
0.628ProCys: 0.628 ± 0.249
4.882ProAsp: 4.882 ± 0.752
3.418ProGlu: 3.418 ± 0.451
1.883ProPhe: 1.883 ± 0.36
4.743ProGly: 4.743 ± 0.613
0.767ProHis: 0.767 ± 0.21
1.255ProIle: 1.255 ± 0.355
2.162ProLys: 2.162 ± 0.475
3.836ProLeu: 3.836 ± 0.571
0.767ProMet: 0.767 ± 0.235
1.046ProAsn: 1.046 ± 0.263
1.953ProPro: 1.953 ± 0.35
0.837ProGln: 0.837 ± 0.246
2.65ProArg: 2.65 ± 0.437
2.511ProSer: 2.511 ± 0.388
3.627ProThr: 3.627 ± 0.437
6.347ProVal: 6.347 ± 0.897
1.046ProTrp: 1.046 ± 0.264
1.534ProTyr: 1.534 ± 0.348
0.0ProXaa: 0.0 ± 0.0
Gln
3.836GlnAla: 3.836 ± 0.495
0.139GlnCys: 0.139 ± 0.088
2.023GlnAsp: 2.023 ± 0.36
0.907GlnGlu: 0.907 ± 0.29
0.488GlnPhe: 0.488 ± 0.197
2.023GlnGly: 2.023 ± 0.419
0.209GlnHis: 0.209 ± 0.135
1.674GlnIle: 1.674 ± 0.294
1.046GlnLys: 1.046 ± 0.292
2.162GlnLeu: 2.162 ± 0.461
0.558GlnMet: 0.558 ± 0.202
0.279GlnAsn: 0.279 ± 0.171
0.767GlnPro: 0.767 ± 0.216
0.139GlnGln: 0.139 ± 0.094
2.023GlnArg: 2.023 ± 0.467
1.744GlnSer: 1.744 ± 0.33
2.999GlnThr: 2.999 ± 0.525
2.441GlnVal: 2.441 ± 0.364
0.349GlnTrp: 0.349 ± 0.181
0.418GlnTyr: 0.418 ± 0.168
0.0GlnXaa: 0.0 ± 0.0
Arg
10.672ArgAla: 10.672 ± 1.484
0.488ArgCys: 0.488 ± 0.223
6.556ArgAsp: 6.556 ± 0.841
4.534ArgGlu: 4.534 ± 0.865
2.371ArgPhe: 2.371 ± 0.381
4.673ArgGly: 4.673 ± 0.5
0.837ArgHis: 0.837 ± 0.255
2.79ArgIle: 2.79 ± 0.409
2.999ArgLys: 2.999 ± 0.445
8.021ArgLeu: 8.021 ± 1.123
1.186ArgMet: 1.186 ± 0.269
1.046ArgAsn: 1.046 ± 0.261
3.557ArgPro: 3.557 ± 0.511
2.441ArgGln: 2.441 ± 0.566
8.649ArgArg: 8.649 ± 1.165
3.766ArgSer: 3.766 ± 0.456
3.627ArgThr: 3.627 ± 0.611
7.254ArgVal: 7.254 ± 0.793
1.186ArgTrp: 1.186 ± 0.275
2.581ArgTyr: 2.581 ± 0.509
0.0ArgXaa: 0.0 ± 0.0
Ser
8.579SerAla: 8.579 ± 1.065
0.07SerCys: 0.07 ± 0.062
2.999SerAsp: 2.999 ± 0.326
2.162SerGlu: 2.162 ± 0.445
1.883SerPhe: 1.883 ± 0.466
5.719SerGly: 5.719 ± 0.907
0.418SerHis: 0.418 ± 0.176
1.534SerIle: 1.534 ± 0.441
1.186SerLys: 1.186 ± 0.264
4.534SerLeu: 4.534 ± 0.462
0.628SerMet: 0.628 ± 0.198
1.325SerAsn: 1.325 ± 0.346
3.139SerPro: 3.139 ± 0.433
1.116SerGln: 1.116 ± 0.21
3.697SerArg: 3.697 ± 0.481
3.278SerSer: 3.278 ± 0.598
4.045SerThr: 4.045 ± 0.527
3.418SerVal: 3.418 ± 0.5
1.465SerTrp: 1.465 ± 0.271
1.395SerTyr: 1.395 ± 0.4
0.0SerXaa: 0.0 ± 0.0
Thr
8.579ThrAla: 8.579 ± 1.29
0.07ThrCys: 0.07 ± 0.069
4.464ThrAsp: 4.464 ± 0.661
3.697ThrGlu: 3.697 ± 0.54
2.162ThrPhe: 2.162 ± 0.357
5.65ThrGly: 5.65 ± 0.748
0.837ThrHis: 0.837 ± 0.26
2.232ThrIle: 2.232 ± 0.441
1.744ThrLys: 1.744 ± 0.361
4.952ThrLeu: 4.952 ± 0.56
0.907ThrMet: 0.907 ± 0.216
0.628ThrAsn: 0.628 ± 0.165
4.324ThrPro: 4.324 ± 0.615
1.325ThrGln: 1.325 ± 0.261
4.952ThrArg: 4.952 ± 0.685
3.976ThrSer: 3.976 ± 0.604
4.464ThrThr: 4.464 ± 0.805
6.696ThrVal: 6.696 ± 0.969
0.628ThrTrp: 0.628 ± 0.234
1.116ThrTyr: 1.116 ± 0.266
0.0ThrXaa: 0.0 ± 0.0
Val
11.927ValAla: 11.927 ± 1.195
0.767ValCys: 0.767 ± 0.305
5.929ValAsp: 5.929 ± 0.631
4.813ValGlu: 4.813 ± 0.567
2.86ValPhe: 2.86 ± 0.625
5.859ValGly: 5.859 ± 1.011
0.767ValHis: 0.767 ± 0.229
3.208ValIle: 3.208 ± 0.486
2.441ValLys: 2.441 ± 0.352
7.812ValLeu: 7.812 ± 0.686
1.604ValMet: 1.604 ± 0.357
2.092ValAsn: 2.092 ± 0.441
5.092ValPro: 5.092 ± 0.563
1.953ValGln: 1.953 ± 0.374
7.184ValArg: 7.184 ± 0.753
4.603ValSer: 4.603 ± 0.6
4.185ValThr: 4.185 ± 0.831
6.417ValVal: 6.417 ± 0.844
1.534ValTrp: 1.534 ± 0.346
2.162ValTyr: 2.162 ± 0.383
0.0ValXaa: 0.0 ± 0.0
Trp
1.813TrpAla: 1.813 ± 0.335
0.07TrpCys: 0.07 ± 0.068
1.325TrpAsp: 1.325 ± 0.251
1.395TrpGlu: 1.395 ± 0.384
0.139TrpPhe: 0.139 ± 0.109
1.674TrpGly: 1.674 ± 0.394
0.418TrpHis: 0.418 ± 0.191
0.628TrpIle: 0.628 ± 0.2
0.488TrpLys: 0.488 ± 0.216
0.907TrpLeu: 0.907 ± 0.24
0.139TrpMet: 0.139 ± 0.093
0.558TrpAsn: 0.558 ± 0.407
0.907TrpPro: 0.907 ± 0.289
0.767TrpGln: 0.767 ± 0.224
2.302TrpArg: 2.302 ± 0.412
1.325TrpSer: 1.325 ± 0.268
1.465TrpThr: 1.465 ± 0.381
0.976TrpVal: 0.976 ± 0.331
0.209TrpTrp: 0.209 ± 0.126
0.558TrpTyr: 0.558 ± 0.186
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.766TyrAla: 3.766 ± 0.443
0.279TyrCys: 0.279 ± 0.147
1.744TyrAsp: 1.744 ± 0.293
1.395TyrGlu: 1.395 ± 0.418
0.697TyrPhe: 0.697 ± 0.247
2.371TyrGly: 2.371 ± 0.471
0.349TyrHis: 0.349 ± 0.158
0.628TyrIle: 0.628 ± 0.211
0.558TyrLys: 0.558 ± 0.209
2.302TyrLeu: 2.302 ± 0.465
0.837TyrMet: 0.837 ± 0.199
0.279TyrAsn: 0.279 ± 0.132
1.186TyrPro: 1.186 ± 0.253
0.349TyrGln: 0.349 ± 0.177
2.092TyrArg: 2.092 ± 0.323
1.255TyrSer: 1.255 ± 0.26
1.674TyrThr: 1.674 ± 0.342
2.302TyrVal: 2.302 ± 0.385
0.767TyrTrp: 0.767 ± 0.245
0.628TyrTyr: 0.628 ± 0.228
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 69 proteins (14338 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski