Gordonia phage PhrostedPhlake

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fairfaxidumvirus; unclassified Fairfaxidumvirus

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 88 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y6EHI8|A0A4Y6EHI8_9CAUD Helix-turn-helix DNA binding protein OS=Gordonia phage PhrostedPhlake OX=2588135 GN=41 PE=4 SV=1
MM1 pKa = 7.46ANDD4 pKa = 4.61TIFEE8 pKa = 4.28LPEE11 pKa = 4.65IPGVTFTASYY21 pKa = 10.84GSGGEE26 pKa = 4.12TGLPSNWIRR35 pKa = 11.84IVGTVEE41 pKa = 3.86NPWYY45 pKa = 10.31DD46 pKa = 3.26PTYY49 pKa = 11.08NYY51 pKa = 11.18GLDD54 pKa = 3.77PSGHH58 pKa = 5.92TEE60 pKa = 3.79MTDD63 pKa = 2.48PWKK66 pKa = 10.72RR67 pKa = 11.84HH68 pKa = 3.86TQFPEE73 pKa = 3.62VLGPMGFEE81 pKa = 4.55GPSIGLPVDD90 pKa = 3.94PPPPPVEE97 pKa = 4.39PEE99 pKa = 3.75PTPDD103 pKa = 3.64IEE105 pKa = 4.54EE106 pKa = 4.49PTDD109 pKa = 3.42GG110 pKa = 4.69

Molecular weight: 11.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y6EHC1|A0A4Y6EHC1_9CAUD Uncharacterized protein OS=Gordonia phage PhrostedPhlake OX=2588135 GN=53 PE=4 SV=1
MM1 pKa = 7.86RR2 pKa = 11.84SGHH5 pKa = 6.09EE6 pKa = 3.96SDD8 pKa = 3.11KK9 pKa = 10.99TRR11 pKa = 11.84ASGHH15 pKa = 4.83YY16 pKa = 8.83VVNNIVPMNNTSNTGKK32 pKa = 10.16SKK34 pKa = 10.62SVPTEE39 pKa = 3.49WQFLVGGFLRR49 pKa = 11.84HH50 pKa = 5.82LRR52 pKa = 11.84AAGQPVTTINTRR64 pKa = 11.84RR65 pKa = 11.84QQIEE69 pKa = 3.89RR70 pKa = 11.84MARR73 pKa = 11.84ALDD76 pKa = 3.84GSPAEE81 pKa = 4.29VTRR84 pKa = 11.84DD85 pKa = 3.57AITDD89 pKa = 3.16YY90 pKa = 11.03HH91 pKa = 7.2AAQTWALEE99 pKa = 3.96TRR101 pKa = 11.84RR102 pKa = 11.84GHH104 pKa = 6.45RR105 pKa = 11.84NAAVSFFAWAHH116 pKa = 5.1SCGHH120 pKa = 6.32IPGNPAQHH128 pKa = 6.15LPIVKK133 pKa = 10.05SSVPAPRR140 pKa = 11.84PAPDD144 pKa = 2.72RR145 pKa = 11.84VVNVALQTPEE155 pKa = 4.25KK156 pKa = 10.03RR157 pKa = 11.84VQLMLRR163 pKa = 11.84LSCEE167 pKa = 3.66LGLRR171 pKa = 11.84RR172 pKa = 11.84AEE174 pKa = 4.07VAVVSTTDD182 pKa = 3.16VEE184 pKa = 4.77DD185 pKa = 4.3GVGGAMLRR193 pKa = 11.84VHH195 pKa = 6.82GKK197 pKa = 9.01GGRR200 pKa = 11.84IRR202 pKa = 11.84VIPISDD208 pKa = 3.84DD209 pKa = 3.19LALRR213 pKa = 11.84IEE215 pKa = 5.0DD216 pKa = 3.65GAAGHH221 pKa = 6.29TPGAPRR227 pKa = 11.84TGYY230 pKa = 10.43LFPGNEE236 pKa = 4.26SGHH239 pKa = 6.53LSPRR243 pKa = 11.84WVGKK247 pKa = 10.12LCADD251 pKa = 4.7ALPGGWTMHH260 pKa = 5.65TLRR263 pKa = 11.84HH264 pKa = 5.69RR265 pKa = 11.84FATRR269 pKa = 11.84AYY271 pKa = 9.41RR272 pKa = 11.84GSRR275 pKa = 11.84NLRR278 pKa = 11.84AVQILLGHH286 pKa = 7.04ASLATTQRR294 pKa = 11.84YY295 pKa = 5.49TAVDD299 pKa = 3.57EE300 pKa = 4.78DD301 pKa = 4.15EE302 pKa = 4.82VRR304 pKa = 11.84AAMTSAAA311 pKa = 4.24

Molecular weight: 33.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

88

0

88

16976

53

1846

192.9

21.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.628 ± 0.539

1.031 ± 0.18

6.916 ± 0.319

5.578 ± 0.233

2.763 ± 0.125

8.683 ± 0.307

2.203 ± 0.159

4.418 ± 0.17

3.187 ± 0.235

7.458 ± 0.252

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.233 ± 0.163

2.945 ± 0.139

6.05 ± 0.282

3.358 ± 0.126

7.522 ± 0.32

5.219 ± 0.158

6.68 ± 0.239

7.782 ± 0.221

2.062 ± 0.119

2.286 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski