Metallosphaera rod-shaped virus 1

Taxonomy: Viruses; Adnaviria; Zilligvirae; Taleaviricota; Tokiviricetes; Ligamenvirales; Rudiviridae; Hoswirudivirus; Hoswirudivirus MRV1

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 27 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3VZ40|A0A6M3VZ40_9VIRU Uncharacterized protein OS=Metallosphaera rod-shaped virus 1 OX=2730618 GN=MRV1_gp23 PE=4 SV=1
MM1 pKa = 7.66SIQYY5 pKa = 8.46ATISSLLAKK14 pKa = 9.78PFQRR18 pKa = 11.84LTSAMWNTAVLTLSDD33 pKa = 3.73IYY35 pKa = 11.1FGGSLIDD42 pKa = 3.51RR43 pKa = 11.84QLFKK47 pKa = 11.16NGSLYY52 pKa = 10.84VPNNITAQVGNFSEE66 pKa = 4.38EE67 pKa = 4.76VYY69 pKa = 11.04VEE71 pKa = 4.01GQPVLTEE78 pKa = 3.65QDD80 pKa = 3.8PIYY83 pKa = 10.46IAGFVGSAIEE93 pKa = 4.01QIQAILNSQFQLYY106 pKa = 9.2EE107 pKa = 4.71AIEE110 pKa = 3.98QMPTDD115 pKa = 4.32VYY117 pKa = 11.46NAIAKK122 pKa = 7.57LTAGTVAQTQSALQQEE138 pKa = 4.55IANVYY143 pKa = 8.23EE144 pKa = 3.87NLYY147 pKa = 10.22QVVIGVDD154 pKa = 3.59NQLKK158 pKa = 8.02TAILPTLYY166 pKa = 10.48LPQLIQNVYY175 pKa = 8.6EE176 pKa = 4.23NIYY179 pKa = 10.33LVSAKK184 pKa = 9.58LTDD187 pKa = 3.48QMVTGLSRR195 pKa = 11.84AINSVADD202 pKa = 3.67TLLYY206 pKa = 10.24ALSYY210 pKa = 10.44IYY212 pKa = 10.78LAMNSLSSTINKK224 pKa = 8.02LTMYY228 pKa = 8.33MSPPAIQGLQLKK240 pKa = 10.22VSTTPSPIYY249 pKa = 10.2SGPALQTVKK258 pKa = 10.56IILQNLSNYY267 pKa = 8.89IVYY270 pKa = 10.19IGNQLYY276 pKa = 10.99NNFPILPGDD285 pKa = 3.92SIEE288 pKa = 4.85FEE290 pKa = 4.72VNNPANVYY298 pKa = 10.0AWATGPATVYY308 pKa = 11.38ALFEE312 pKa = 4.27VLL314 pKa = 5.17

Molecular weight: 34.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3VXW7|A0A6M3VXW7_9VIRU Uncharacterized protein OS=Metallosphaera rod-shaped virus 1 OX=2730618 GN=MRV1_gp20 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.04GHH5 pKa = 5.62TPRR8 pKa = 11.84SYY10 pKa = 9.84SQRR13 pKa = 11.84YY14 pKa = 8.04GKK16 pKa = 9.56WNAKK20 pKa = 7.96FTAFSNPTVASTILTNVAPTAQEE43 pKa = 3.84NFQTNVPRR51 pKa = 11.84FTSVNEE57 pKa = 3.84QVSAVLTQYY66 pKa = 11.31GVTGPSRR73 pKa = 11.84AIYY76 pKa = 9.48QGYY79 pKa = 8.18GLKK82 pKa = 10.09VARR85 pKa = 11.84ALNRR89 pKa = 11.84IGAGPALTNMVAGLKK104 pKa = 9.92AYY106 pKa = 9.71YY107 pKa = 10.36VSAFGANPQILDD119 pKa = 3.4AVTNIILGSPNGYY132 pKa = 10.06VSS134 pKa = 3.24

Molecular weight: 14.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

27

0

27

6317

48

1115

234.0

26.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.285 ± 0.384

0.744 ± 0.193

5.224 ± 0.381

6.411 ± 0.547

5.066 ± 0.268

4.622 ± 0.393

1.33 ± 0.22

8.944 ± 0.466

7.519 ± 0.611

9.878 ± 0.64

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.741 ± 0.165

6.095 ± 0.419

4.005 ± 0.393

4.781 ± 0.389

3.182 ± 0.339

6.142 ± 0.34

5.018 ± 0.364

6.522 ± 0.343

0.76 ± 0.115

5.731 ± 0.373
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski