Enterobacteria phage SfI

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 7.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1FPM7|M1FPM7_9CAUD CII protein OS=Enterobacteria phage SfI OX=1225789 GN=CII PE=4 SV=1
MM1 pKa = 7.56EE2 pKa = 4.6EE3 pKa = 3.77EE4 pKa = 3.94FEE6 pKa = 4.21EE7 pKa = 5.35FEE9 pKa = 4.23EE10 pKa = 4.87HH11 pKa = 6.47PQDD14 pKa = 5.15VMEE17 pKa = 4.57QYY19 pKa = 10.87QDD21 pKa = 3.33YY22 pKa = 9.44PYY24 pKa = 10.79DD25 pKa = 3.5YY26 pKa = 10.74DD27 pKa = 3.8YY28 pKa = 12.14

Molecular weight: 3.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1FJ65|M1FJ65_9CAUD Antitermination protein OS=Enterobacteria phage SfI OX=1225789 GN=CIII PE=4 SV=1
MM1 pKa = 7.55AMKK4 pKa = 10.22HH5 pKa = 5.08PHH7 pKa = 6.27DD8 pKa = 5.1NIRR11 pKa = 11.84VGAITFVYY19 pKa = 10.4SVTKK23 pKa = 10.38RR24 pKa = 11.84GWVFPGLSVIRR35 pKa = 11.84NPLKK39 pKa = 10.16AQRR42 pKa = 11.84LAEE45 pKa = 4.43EE46 pKa = 4.41INNKK50 pKa = 9.68RR51 pKa = 11.84EE52 pKa = 4.24SVV54 pKa = 3.31

Molecular weight: 6.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

11898

18

611

183.0

20.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.993 ± 0.323

1.471 ± 0.175

5.682 ± 0.272

5.984 ± 0.284

3.673 ± 0.304

6.867 ± 0.389

1.79 ± 0.173

5.715 ± 0.342

5.892 ± 0.383

8.548 ± 0.323

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.79 ± 0.208

4.328 ± 0.19

4.244 ± 0.162

3.866 ± 0.203

6.455 ± 0.439

6.161 ± 0.288

6.11 ± 0.335

6.497 ± 0.292

1.933 ± 0.177

3.001 ± 0.195
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski