Haematobacter massiliensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Haematobacter

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3806 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A086YAP9|A0A086YAP9_9RHOB Putative membrane protein insertion efficiency factor OS=Haematobacter massiliensis OX=195105 GN=yidD PE=3 SV=1
MM1 pKa = 7.61IFFTPRR7 pKa = 11.84SAIPGVLAAVALLSSALPGLAAGFTAEE34 pKa = 3.92QAEE37 pKa = 4.36AGKK40 pKa = 8.16TAYY43 pKa = 9.89NANCAQCHH51 pKa = 4.54GMQLEE56 pKa = 4.63GPEE59 pKa = 4.2APGLVGPDD67 pKa = 3.54IMANWDD73 pKa = 4.0TPGGLYY79 pKa = 10.72DD80 pKa = 5.08FISVAMPPSAPGQLGAATYY99 pKa = 10.78LDD101 pKa = 4.03IIAYY105 pKa = 9.55IMAFNGAQPGPDD117 pKa = 3.31PLVEE121 pKa = 4.79DD122 pKa = 4.39EE123 pKa = 5.07EE124 pKa = 4.37KK125 pKa = 10.88LAAISLTAEE134 pKa = 4.16TAAGPAGGAAPASDD148 pKa = 4.74SMAADD153 pKa = 3.56TNVPQAFTWGKK164 pKa = 8.6PLPGGPAVDD173 pKa = 3.86TMQTSAAAASSIPQAFTWGKK193 pKa = 9.15PLPTVEE199 pKa = 4.04

Molecular weight: 19.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A086YAW2|A0A086YAW2_9RHOB Uncharacterized protein OS=Haematobacter massiliensis OX=195105 GN=CN97_10420 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNLVRR13 pKa = 11.84KK14 pKa = 9.18RR15 pKa = 11.84RR16 pKa = 11.84HH17 pKa = 4.42GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.21GGQRR30 pKa = 11.84VLNARR35 pKa = 11.84RR36 pKa = 11.84ARR38 pKa = 11.84GRR40 pKa = 11.84KK41 pKa = 8.97VLSAA45 pKa = 4.11

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3806

0

3806

1175726

37

2979

308.9

33.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.888 ± 0.051

0.769 ± 0.01

5.523 ± 0.028

6.035 ± 0.032

3.581 ± 0.025

8.869 ± 0.037

1.946 ± 0.017

4.959 ± 0.028

2.621 ± 0.028

10.491 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.654 ± 0.019

2.271 ± 0.021

5.446 ± 0.024

2.96 ± 0.019

7.638 ± 0.04

5.058 ± 0.026

5.394 ± 0.023

7.408 ± 0.031

1.406 ± 0.016

2.082 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski