Amino acid dipepetide frequency for Bacillus phage OmnioDeoPrimus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.028AlaAla: 4.028 ± 0.539
0.321AlaCys: 0.321 ± 0.096
3.467AlaAsp: 3.467 ± 0.271
4.108AlaGlu: 4.108 ± 0.268
2.665AlaPhe: 2.665 ± 0.259
3.828AlaGly: 3.828 ± 0.428
1.182AlaHis: 1.182 ± 0.139
4.449AlaIle: 4.449 ± 0.306
4.87AlaLys: 4.87 ± 0.334
5.111AlaLeu: 5.111 ± 0.281
1.864AlaMet: 1.864 ± 0.226
3.307AlaAsn: 3.307 ± 0.374
1.884AlaPro: 1.884 ± 0.202
2.425AlaGln: 2.425 ± 0.218
2.585AlaArg: 2.585 ± 0.235
2.926AlaSer: 2.926 ± 0.285
3.828AlaThr: 3.828 ± 0.421
4.068AlaVal: 4.068 ± 0.343
0.782AlaTrp: 0.782 ± 0.137
2.665AlaTyr: 2.665 ± 0.263
0.0AlaXaa: 0.0 ± 0.0
Cys
0.361CysAla: 0.361 ± 0.089
0.12CysCys: 0.12 ± 0.053
0.661CysAsp: 0.661 ± 0.136
0.742CysGlu: 0.742 ± 0.129
0.441CysPhe: 0.441 ± 0.103
0.802CysGly: 0.802 ± 0.146
0.24CysHis: 0.24 ± 0.073
0.521CysIle: 0.521 ± 0.101
0.721CysLys: 0.721 ± 0.121
0.581CysLeu: 0.581 ± 0.121
0.24CysMet: 0.24 ± 0.087
0.481CysAsn: 0.481 ± 0.125
0.361CysPro: 0.361 ± 0.095
0.281CysGln: 0.281 ± 0.065
0.301CysArg: 0.301 ± 0.067
0.501CysSer: 0.501 ± 0.093
0.721CysThr: 0.721 ± 0.121
0.481CysVal: 0.481 ± 0.096
0.18CysTrp: 0.18 ± 0.06
0.401CysTyr: 0.401 ± 0.097
0.0CysXaa: 0.0 ± 0.0
Asp
4.609AspAla: 4.609 ± 0.404
0.721AspCys: 0.721 ± 0.131
4.209AspAsp: 4.209 ± 0.368
5.451AspGlu: 5.451 ± 0.417
2.966AspPhe: 2.966 ± 0.212
3.988AspGly: 3.988 ± 0.349
0.621AspHis: 0.621 ± 0.109
5.271AspIle: 5.271 ± 0.309
5.852AspLys: 5.852 ± 0.371
5.01AspLeu: 5.01 ± 0.332
2.305AspMet: 2.305 ± 0.263
3.066AspAsn: 3.066 ± 0.196
1.643AspPro: 1.643 ± 0.247
0.942AspGln: 0.942 ± 0.143
2.886AspArg: 2.886 ± 0.209
3.086AspSer: 3.086 ± 0.236
3.708AspThr: 3.708 ± 0.335
5.09AspVal: 5.09 ± 0.321
0.822AspTrp: 0.822 ± 0.12
3.287AspTyr: 3.287 ± 0.285
0.0AspXaa: 0.0 ± 0.0
Glu
4.249GluAla: 4.249 ± 0.328
0.721GluCys: 0.721 ± 0.16
4.81GluAsp: 4.81 ± 0.339
9.019GluGlu: 9.019 ± 0.715
3.527GluPhe: 3.527 ± 0.256
4.93GluGly: 4.93 ± 0.287
1.603GluHis: 1.603 ± 0.228
5.551GluIle: 5.551 ± 0.335
5.812GluLys: 5.812 ± 0.475
8.037GluLeu: 8.037 ± 0.45
3.167GluMet: 3.167 ± 0.218
3.447GluAsn: 3.447 ± 0.232
1.924GluPro: 1.924 ± 0.213
3.046GluGln: 3.046 ± 0.203
3.507GluArg: 3.507 ± 0.295
4.269GluSer: 4.269 ± 0.289
3.928GluThr: 3.928 ± 0.305
6.253GluVal: 6.253 ± 0.451
0.982GluTrp: 0.982 ± 0.175
3.267GluTyr: 3.267 ± 0.258
0.0GluXaa: 0.0 ± 0.0
Phe
2.305PheAla: 2.305 ± 0.21
0.481PheCys: 0.481 ± 0.093
3.327PheAsp: 3.327 ± 0.287
2.565PheGlu: 2.565 ± 0.222
1.744PhePhe: 1.744 ± 0.206
2.185PheGly: 2.185 ± 0.214
0.842PheHis: 0.842 ± 0.135
2.726PheIle: 2.726 ± 0.219
3.126PheLys: 3.126 ± 0.262
3.046PheLeu: 3.046 ± 0.254
1.423PheMet: 1.423 ± 0.163
2.645PheAsn: 2.645 ± 0.238
1.082PhePro: 1.082 ± 0.14
1.182PheGln: 1.182 ± 0.141
1.643PheArg: 1.643 ± 0.174
1.884PheSer: 1.884 ± 0.199
3.367PheThr: 3.367 ± 0.262
2.786PheVal: 2.786 ± 0.204
0.421PheTrp: 0.421 ± 0.094
1.663PheTyr: 1.663 ± 0.172
0.0PheXaa: 0.0 ± 0.0
Gly
3.668GlyAla: 3.668 ± 0.548
0.521GlyCys: 0.521 ± 0.108
3.106GlyAsp: 3.106 ± 0.258
4.81GlyGlu: 4.81 ± 0.332
2.786GlyPhe: 2.786 ± 0.191
4.93GlyGly: 4.93 ± 0.687
0.842GlyHis: 0.842 ± 0.135
3.708GlyIle: 3.708 ± 0.36
4.91GlyLys: 4.91 ± 0.302
4.229GlyLeu: 4.229 ± 0.335
1.804GlyMet: 1.804 ± 0.198
3.046GlyAsn: 3.046 ± 0.343
0.561GlyPro: 0.561 ± 0.09
1.764GlyGln: 1.764 ± 0.285
2.826GlyArg: 2.826 ± 0.219
3.648GlySer: 3.648 ± 0.35
4.309GlyThr: 4.309 ± 0.383
5.612GlyVal: 5.612 ± 0.304
1.062GlyTrp: 1.062 ± 0.147
3.407GlyTyr: 3.407 ± 0.29
0.0GlyXaa: 0.0 ± 0.0
His
0.902HisAla: 0.902 ± 0.165
0.2HisCys: 0.2 ± 0.054
1.122HisAsp: 1.122 ± 0.168
1.202HisGlu: 1.202 ± 0.172
0.661HisPhe: 0.661 ± 0.127
1.082HisGly: 1.082 ± 0.173
0.321HisHis: 0.321 ± 0.087
1.323HisIle: 1.323 ± 0.16
1.503HisLys: 1.503 ± 0.182
1.343HisLeu: 1.343 ± 0.183
0.681HisMet: 0.681 ± 0.106
1.182HisAsn: 1.182 ± 0.166
0.581HisPro: 0.581 ± 0.105
0.681HisGln: 0.681 ± 0.105
0.681HisArg: 0.681 ± 0.122
0.882HisSer: 0.882 ± 0.147
1.182HisThr: 1.182 ± 0.145
1.824HisVal: 1.824 ± 0.23
0.281HisTrp: 0.281 ± 0.078
0.982HisTyr: 0.982 ± 0.158
0.0HisXaa: 0.0 ± 0.0
Ile
4.209IleAla: 4.209 ± 0.274
0.762IleCys: 0.762 ± 0.146
4.99IleAsp: 4.99 ± 0.309
5.972IleGlu: 5.972 ± 0.312
1.844IlePhe: 1.844 ± 0.173
3.848IleGly: 3.848 ± 0.25
1.042IleHis: 1.042 ± 0.158
4.289IleIle: 4.289 ± 0.349
6.153IleLys: 6.153 ± 0.316
4.389IleLeu: 4.389 ± 0.363
2.084IleMet: 2.084 ± 0.23
3.547IleAsn: 3.547 ± 0.241
1.984IlePro: 1.984 ± 0.224
2.185IleGln: 2.185 ± 0.216
3.046IleArg: 3.046 ± 0.24
4.028IleSer: 4.028 ± 0.281
5.07IleThr: 5.07 ± 0.35
4.469IleVal: 4.469 ± 0.385
0.721IleTrp: 0.721 ± 0.109
2.265IleTyr: 2.265 ± 0.212
0.0IleXaa: 0.0 ± 0.0
Lys
4.65LysAla: 4.65 ± 0.362
0.641LysCys: 0.641 ± 0.118
5.291LysAsp: 5.291 ± 0.316
8.197LysGlu: 8.197 ± 0.554
2.706LysPhe: 2.706 ± 0.286
4.69LysGly: 4.69 ± 0.373
1.884LysHis: 1.884 ± 0.196
4.149LysIle: 4.149 ± 0.324
5.571LysLys: 5.571 ± 0.405
6.694LysLeu: 6.694 ± 0.384
2.525LysMet: 2.525 ± 0.223
4.068LysAsn: 4.068 ± 0.302
2.285LysPro: 2.285 ± 0.236
3.507LysGln: 3.507 ± 0.3
3.387LysArg: 3.387 ± 0.308
3.768LysSer: 3.768 ± 0.333
4.369LysThr: 4.369 ± 0.316
5.692LysVal: 5.692 ± 0.336
1.002LysTrp: 1.002 ± 0.12
2.886LysTyr: 2.886 ± 0.283
0.0LysXaa: 0.0 ± 0.0
Leu
5.111LeuAla: 5.111 ± 0.343
0.661LeuCys: 0.661 ± 0.146
5.892LeuAsp: 5.892 ± 0.294
6.994LeuGlu: 6.994 ± 0.472
2.605LeuPhe: 2.605 ± 0.22
4.73LeuGly: 4.73 ± 0.365
1.142LeuHis: 1.142 ± 0.155
4.97LeuIle: 4.97 ± 0.316
5.632LeuLys: 5.632 ± 0.33
6.073LeuLeu: 6.073 ± 0.367
2.185LeuMet: 2.185 ± 0.212
4.149LeuAsn: 4.149 ± 0.27
2.786LeuPro: 2.786 ± 0.237
2.906LeuGln: 2.906 ± 0.234
3.848LeuArg: 3.848 ± 0.303
4.75LeuSer: 4.75 ± 0.279
5.271LeuThr: 5.271 ± 0.343
5.511LeuVal: 5.511 ± 0.385
0.882LeuTrp: 0.882 ± 0.148
3.106LeuTyr: 3.106 ± 0.292
0.0LeuXaa: 0.0 ± 0.0
Met
1.784MetAla: 1.784 ± 0.183
0.361MetCys: 0.361 ± 0.086
1.984MetAsp: 1.984 ± 0.19
2.064MetGlu: 2.064 ± 0.193
1.523MetPhe: 1.523 ± 0.176
1.864MetGly: 1.864 ± 0.242
0.521MetHis: 0.521 ± 0.106
1.844MetIle: 1.844 ± 0.197
2.866MetLys: 2.866 ± 0.25
2.425MetLeu: 2.425 ± 0.192
0.942MetMet: 0.942 ± 0.145
1.964MetAsn: 1.964 ± 0.191
0.742MetPro: 0.742 ± 0.138
0.842MetGln: 0.842 ± 0.12
1.704MetArg: 1.704 ± 0.184
2.465MetSer: 2.465 ± 0.223
2.605MetThr: 2.605 ± 0.242
1.704MetVal: 1.704 ± 0.187
0.341MetTrp: 0.341 ± 0.087
1.563MetTyr: 1.563 ± 0.194
0.0MetXaa: 0.0 ± 0.0
Asn
3.086AsnAla: 3.086 ± 0.297
0.421AsnCys: 0.421 ± 0.084
3.026AsnAsp: 3.026 ± 0.252
3.407AsnGlu: 3.407 ± 0.243
1.884AsnPhe: 1.884 ± 0.196
4.289AsnGly: 4.289 ± 0.301
1.122AsnHis: 1.122 ± 0.139
3.908AsnIle: 3.908 ± 0.259
4.549AsnLys: 4.549 ± 0.32
3.587AsnLeu: 3.587 ± 0.294
2.044AsnMet: 2.044 ± 0.182
3.247AsnAsn: 3.247 ± 0.297
2.044AsnPro: 2.044 ± 0.259
1.744AsnGln: 1.744 ± 0.167
2.565AsnArg: 2.565 ± 0.215
2.846AsnSer: 2.846 ± 0.254
3.267AsnThr: 3.267 ± 0.264
3.547AsnVal: 3.547 ± 0.261
0.621AsnTrp: 0.621 ± 0.091
2.245AsnTyr: 2.245 ± 0.212
0.0AsnXaa: 0.0 ± 0.0
Pro
1.924ProAla: 1.924 ± 0.198
0.281ProCys: 0.281 ± 0.082
2.004ProAsp: 2.004 ± 0.234
2.665ProGlu: 2.665 ± 0.204
1.343ProPhe: 1.343 ± 0.175
0.02ProGly: 0.02 ± 0.022
0.581ProHis: 0.581 ± 0.114
1.443ProIle: 1.443 ± 0.231
2.104ProLys: 2.104 ± 0.269
1.944ProLeu: 1.944 ± 0.239
0.701ProMet: 0.701 ± 0.113
1.683ProAsn: 1.683 ± 0.207
0.721ProPro: 0.721 ± 0.17
0.902ProGln: 0.902 ± 0.132
1.263ProArg: 1.263 ± 0.157
1.944ProSer: 1.944 ± 0.219
2.385ProThr: 2.385 ± 0.287
2.004ProVal: 2.004 ± 0.247
0.2ProTrp: 0.2 ± 0.055
1.403ProTyr: 1.403 ± 0.175
0.0ProXaa: 0.0 ± 0.0
Gln
2.164GlnAla: 2.164 ± 0.273
0.301GlnCys: 0.301 ± 0.085
2.004GlnAsp: 2.004 ± 0.188
2.746GlnGlu: 2.746 ± 0.243
1.263GlnPhe: 1.263 ± 0.144
1.884GlnGly: 1.884 ± 0.245
0.822GlnHis: 0.822 ± 0.126
1.904GlnIle: 1.904 ± 0.164
1.964GlnLys: 1.964 ± 0.223
3.287GlnLeu: 3.287 ± 0.222
1.022GlnMet: 1.022 ± 0.166
1.583GlnAsn: 1.583 ± 0.177
0.922GlnPro: 0.922 ± 0.129
1.323GlnGln: 1.323 ± 0.233
1.523GlnArg: 1.523 ± 0.185
1.924GlnSer: 1.924 ± 0.202
1.944GlnThr: 1.944 ± 0.196
2.325GlnVal: 2.325 ± 0.236
0.381GlnTrp: 0.381 ± 0.09
1.483GlnTyr: 1.483 ± 0.143
0.0GlnXaa: 0.0 ± 0.0
Arg
2.886ArgAla: 2.886 ± 0.245
0.341ArgCys: 0.341 ± 0.09
2.766ArgAsp: 2.766 ± 0.288
3.587ArgGlu: 3.587 ± 0.279
2.064ArgPhe: 2.064 ± 0.198
2.545ArgGly: 2.545 ± 0.227
0.882ArgHis: 0.882 ± 0.136
3.026ArgIle: 3.026 ± 0.289
3.227ArgLys: 3.227 ± 0.245
3.587ArgLeu: 3.587 ± 0.271
1.744ArgMet: 1.744 ± 0.182
2.686ArgAsn: 2.686 ± 0.231
1.122ArgPro: 1.122 ± 0.138
1.563ArgGln: 1.563 ± 0.184
1.844ArgArg: 1.844 ± 0.192
2.024ArgSer: 2.024 ± 0.188
2.465ArgThr: 2.465 ± 0.221
3.467ArgVal: 3.467 ± 0.237
0.501ArgTrp: 0.501 ± 0.089
1.964ArgTyr: 1.964 ± 0.188
0.0ArgXaa: 0.0 ± 0.0
Ser
3.467SerAla: 3.467 ± 0.276
0.401SerCys: 0.401 ± 0.089
3.187SerAsp: 3.187 ± 0.247
3.868SerGlu: 3.868 ± 0.292
2.766SerPhe: 2.766 ± 0.227
4.129SerGly: 4.129 ± 0.429
1.002SerHis: 1.002 ± 0.159
4.209SerIle: 4.209 ± 0.347
3.948SerLys: 3.948 ± 0.306
4.509SerLeu: 4.509 ± 0.289
1.864SerMet: 1.864 ± 0.205
2.886SerAsn: 2.886 ± 0.275
1.483SerPro: 1.483 ± 0.209
1.804SerGln: 1.804 ± 0.186
2.585SerArg: 2.585 ± 0.186
3.507SerSer: 3.507 ± 0.366
3.627SerThr: 3.627 ± 0.302
3.227SerVal: 3.227 ± 0.24
0.481SerTrp: 0.481 ± 0.093
2.465SerTyr: 2.465 ± 0.229
0.0SerXaa: 0.0 ± 0.0
Thr
4.349ThrAla: 4.349 ± 0.458
0.521ThrCys: 0.521 ± 0.12
4.209ThrAsp: 4.209 ± 0.379
4.569ThrGlu: 4.569 ± 0.348
3.207ThrPhe: 3.207 ± 0.317
4.67ThrGly: 4.67 ± 0.359
1.223ThrHis: 1.223 ± 0.176
4.529ThrIle: 4.529 ± 0.326
4.569ThrLys: 4.569 ± 0.297
5.211ThrLeu: 5.211 ± 0.33
1.804ThrMet: 1.804 ± 0.184
3.407ThrAsn: 3.407 ± 0.34
2.325ThrPro: 2.325 ± 0.241
1.623ThrGln: 1.623 ± 0.252
3.006ThrArg: 3.006 ± 0.263
3.527ThrSer: 3.527 ± 0.297
3.648ThrThr: 3.648 ± 0.331
4.95ThrVal: 4.95 ± 0.37
0.501ThrTrp: 0.501 ± 0.112
2.826ThrTyr: 2.826 ± 0.265
0.0ThrXaa: 0.0 ± 0.0
Val
3.808ValAla: 3.808 ± 0.235
0.842ValCys: 0.842 ± 0.153
5.171ValAsp: 5.171 ± 0.291
5.612ValGlu: 5.612 ± 0.385
2.505ValPhe: 2.505 ± 0.219
3.928ValGly: 3.928 ± 0.325
1.623ValHis: 1.623 ± 0.212
4.81ValIle: 4.81 ± 0.313
6.313ValLys: 6.313 ± 0.42
5.772ValLeu: 5.772 ± 0.378
2.004ValMet: 2.004 ± 0.201
3.928ValAsn: 3.928 ± 0.328
1.924ValPro: 1.924 ± 0.263
2.325ValGln: 2.325 ± 0.185
2.806ValArg: 2.806 ± 0.23
3.988ValSer: 3.988 ± 0.285
5.391ValThr: 5.391 ± 0.399
5.171ValVal: 5.171 ± 0.391
0.621ValTrp: 0.621 ± 0.11
3.648ValTyr: 3.648 ± 0.284
0.0ValXaa: 0.0 ± 0.0
Trp
0.742TrpAla: 0.742 ± 0.116
0.1TrpCys: 0.1 ± 0.048
1.102TrpAsp: 1.102 ± 0.15
1.062TrpGlu: 1.062 ± 0.149
0.461TrpPhe: 0.461 ± 0.099
0.681TrpGly: 0.681 ± 0.122
0.22TrpHis: 0.22 ± 0.059
0.862TrpIle: 0.862 ± 0.128
0.762TrpLys: 0.762 ± 0.108
0.782TrpLeu: 0.782 ± 0.13
0.361TrpMet: 0.361 ± 0.085
0.521TrpAsn: 0.521 ± 0.102
0.0TrpPro: 0.0 ± 0.0
0.341TrpGln: 0.341 ± 0.093
0.341TrpArg: 0.341 ± 0.077
0.661TrpSer: 0.661 ± 0.123
0.721TrpThr: 0.721 ± 0.158
0.862TrpVal: 0.862 ± 0.127
0.14TrpTrp: 0.14 ± 0.057
0.721TrpTyr: 0.721 ± 0.112
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.064TyrAla: 2.064 ± 0.193
0.421TyrCys: 0.421 ± 0.091
3.207TyrAsp: 3.207 ± 0.308
3.407TyrGlu: 3.407 ± 0.23
1.483TyrPhe: 1.483 ± 0.177
2.525TyrGly: 2.525 ± 0.229
0.902TyrHis: 0.902 ± 0.134
3.407TyrIle: 3.407 ± 0.259
3.527TyrLys: 3.527 ± 0.271
3.587TyrLeu: 3.587 ± 0.286
1.323TyrMet: 1.323 ± 0.173
2.605TyrAsn: 2.605 ± 0.274
1.142TyrPro: 1.142 ± 0.139
1.383TyrGln: 1.383 ± 0.181
1.944TyrArg: 1.944 ± 0.198
2.786TyrSer: 2.786 ± 0.206
2.866TyrThr: 2.866 ± 0.294
3.146TyrVal: 3.146 ± 0.266
0.521TyrTrp: 0.521 ± 0.112
2.144TyrTyr: 2.144 ± 0.23
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 297 proteins (49898 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski