Amino acid dipepetide frequency for Flavobacterium phage vB_FspP_elemoA_3-5A

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.387AlaAla: 2.387 ± 0.627
0.519AlaCys: 0.519 ± 0.239
2.543AlaAsp: 2.543 ± 0.36
4.099AlaGlu: 4.099 ± 0.695
3.01AlaPhe: 3.01 ± 0.467
3.062AlaGly: 3.062 ± 0.508
0.726AlaHis: 0.726 ± 0.153
5.033AlaIle: 5.033 ± 0.704
5.241AlaLys: 5.241 ± 0.835
4.151AlaLeu: 4.151 ± 0.571
1.038AlaMet: 1.038 ± 0.266
3.788AlaAsn: 3.788 ± 0.535
1.142AlaPro: 1.142 ± 0.25
1.816AlaGln: 1.816 ± 0.388
2.128AlaArg: 2.128 ± 0.261
4.255AlaSer: 4.255 ± 0.593
3.062AlaThr: 3.062 ± 0.454
3.269AlaVal: 3.269 ± 0.466
0.104AlaTrp: 0.104 ± 0.083
2.231AlaTyr: 2.231 ± 0.321
0.0AlaXaa: 0.0 ± 0.0
Cys
0.259CysAla: 0.259 ± 0.158
0.052CysCys: 0.052 ± 0.068
0.571CysAsp: 0.571 ± 0.193
0.726CysGlu: 0.726 ± 0.27
0.363CysPhe: 0.363 ± 0.13
0.363CysGly: 0.363 ± 0.149
0.259CysHis: 0.259 ± 0.13
0.571CysIle: 0.571 ± 0.202
0.571CysLys: 0.571 ± 0.197
0.778CysLeu: 0.778 ± 0.272
0.156CysMet: 0.156 ± 0.086
0.311CysAsn: 0.311 ± 0.159
0.156CysPro: 0.156 ± 0.128
0.156CysGln: 0.156 ± 0.092
0.363CysArg: 0.363 ± 0.154
0.208CysSer: 0.208 ± 0.112
0.467CysThr: 0.467 ± 0.181
0.363CysVal: 0.363 ± 0.17
0.104CysTrp: 0.104 ± 0.085
0.311CysTyr: 0.311 ± 0.159
0.0CysXaa: 0.0 ± 0.0
Asp
3.996AspAla: 3.996 ± 0.558
0.519AspCys: 0.519 ± 0.206
4.255AspAsp: 4.255 ± 0.474
4.203AspGlu: 4.203 ± 0.635
4.515AspPhe: 4.515 ± 0.451
4.151AspGly: 4.151 ± 0.508
0.415AspHis: 0.415 ± 0.163
5.085AspIle: 5.085 ± 0.514
7.057AspLys: 7.057 ± 0.825
6.123AspLeu: 6.123 ± 0.533
1.816AspMet: 1.816 ± 0.273
5.085AspAsn: 5.085 ± 0.628
1.142AspPro: 1.142 ± 0.221
1.401AspGln: 1.401 ± 0.229
2.128AspArg: 2.128 ± 0.331
4.774AspSer: 4.774 ± 0.619
3.944AspThr: 3.944 ± 0.502
3.996AspVal: 3.996 ± 0.377
0.882AspTrp: 0.882 ± 0.274
2.958AspTyr: 2.958 ± 0.593
0.0AspXaa: 0.0 ± 0.0
Glu
3.996GluAla: 3.996 ± 0.587
0.259GluCys: 0.259 ± 0.157
5.552GluAsp: 5.552 ± 0.557
6.071GluGlu: 6.071 ± 0.802
4.463GluPhe: 4.463 ± 0.67
4.463GluGly: 4.463 ± 0.474
0.778GluHis: 0.778 ± 0.255
6.071GluIle: 6.071 ± 0.527
5.241GluLys: 5.241 ± 0.691
6.902GluLeu: 6.902 ± 0.71
1.557GluMet: 1.557 ± 0.28
4.099GluAsn: 4.099 ± 0.609
1.245GluPro: 1.245 ± 0.279
2.646GluGln: 2.646 ± 0.479
2.387GluArg: 2.387 ± 0.309
5.137GluSer: 5.137 ± 0.485
3.062GluThr: 3.062 ± 0.496
5.189GluVal: 5.189 ± 0.456
0.83GluTrp: 0.83 ± 0.2
3.684GluTyr: 3.684 ± 0.396
0.0GluXaa: 0.0 ± 0.0
Phe
1.609PheAla: 1.609 ± 0.297
0.519PheCys: 0.519 ± 0.174
3.788PheAsp: 3.788 ± 0.384
3.944PheGlu: 3.944 ± 0.489
1.764PhePhe: 1.764 ± 0.269
2.439PheGly: 2.439 ± 0.413
0.778PheHis: 0.778 ± 0.192
3.529PheIle: 3.529 ± 0.384
4.203PheLys: 4.203 ± 0.588
3.425PheLeu: 3.425 ± 0.607
1.245PheMet: 1.245 ± 0.244
4.359PheAsn: 4.359 ± 0.435
0.778PhePro: 0.778 ± 0.206
1.194PheGln: 1.194 ± 0.203
1.297PheArg: 1.297 ± 0.285
3.684PheSer: 3.684 ± 0.357
2.854PheThr: 2.854 ± 0.491
1.868PheVal: 1.868 ± 0.259
0.415PheTrp: 0.415 ± 0.153
1.764PheTyr: 1.764 ± 0.346
0.0PheXaa: 0.0 ± 0.0
Gly
2.595GlyAla: 2.595 ± 0.459
0.259GlyCys: 0.259 ± 0.118
3.581GlyAsp: 3.581 ± 0.391
3.373GlyGlu: 3.373 ± 0.415
2.854GlyPhe: 2.854 ± 0.349
4.203GlyGly: 4.203 ± 0.493
0.675GlyHis: 0.675 ± 0.252
5.085GlyIle: 5.085 ± 0.577
4.826GlyLys: 4.826 ± 0.606
5.033GlyLeu: 5.033 ± 0.703
1.453GlyMet: 1.453 ± 0.208
3.425GlyAsn: 3.425 ± 0.534
1.09GlyPro: 1.09 ± 0.433
1.661GlyGln: 1.661 ± 0.25
2.439GlyArg: 2.439 ± 0.259
4.411GlySer: 4.411 ± 0.59
4.151GlyThr: 4.151 ± 0.675
4.463GlyVal: 4.463 ± 0.74
0.467GlyTrp: 0.467 ± 0.159
3.321GlyTyr: 3.321 ± 0.346
0.0GlyXaa: 0.0 ± 0.0
His
0.519HisAla: 0.519 ± 0.198
0.259HisCys: 0.259 ± 0.155
0.778HisAsp: 0.778 ± 0.235
0.415HisGlu: 0.415 ± 0.158
0.519HisPhe: 0.519 ± 0.183
0.415HisGly: 0.415 ± 0.136
0.415HisHis: 0.415 ± 0.234
1.142HisIle: 1.142 ± 0.284
1.453HisLys: 1.453 ± 0.356
0.675HisLeu: 0.675 ± 0.208
0.052HisMet: 0.052 ± 0.052
0.778HisAsn: 0.778 ± 0.207
0.571HisPro: 0.571 ± 0.184
0.623HisGln: 0.623 ± 0.157
0.363HisArg: 0.363 ± 0.143
0.934HisSer: 0.934 ± 0.211
0.519HisThr: 0.519 ± 0.155
0.208HisVal: 0.208 ± 0.129
0.208HisTrp: 0.208 ± 0.108
0.623HisTyr: 0.623 ± 0.229
0.0HisXaa: 0.0 ± 0.0
Ile
4.411IleAla: 4.411 ± 0.48
0.363IleCys: 0.363 ± 0.178
7.109IleAsp: 7.109 ± 0.522
6.019IleGlu: 6.019 ± 0.532
2.646IlePhe: 2.646 ± 0.474
3.944IleGly: 3.944 ± 0.475
1.038IleHis: 1.038 ± 0.229
5.5IleIle: 5.5 ± 0.551
7.42IleLys: 7.42 ± 0.753
4.93IleLeu: 4.93 ± 0.524
1.142IleMet: 1.142 ± 0.319
6.59IleAsn: 6.59 ± 0.572
1.816IlePro: 1.816 ± 0.318
2.491IleGln: 2.491 ± 0.433
2.491IleArg: 2.491 ± 0.364
6.279IleSer: 6.279 ± 0.649
4.67IleThr: 4.67 ± 0.546
5.293IleVal: 5.293 ± 0.626
0.623IleTrp: 0.623 ± 0.143
2.75IleTyr: 2.75 ± 0.4
0.0IleXaa: 0.0 ± 0.0
Lys
5.812LysAla: 5.812 ± 0.895
0.571LysCys: 0.571 ± 0.214
6.642LysAsp: 6.642 ± 0.613
8.043LysGlu: 8.043 ± 0.724
2.958LysPhe: 2.958 ± 0.399
4.93LysGly: 4.93 ± 0.515
1.038LysHis: 1.038 ± 0.251
7.109LysIle: 7.109 ± 0.711
8.614LysLys: 8.614 ± 1.009
7.369LysLeu: 7.369 ± 0.978
2.906LysMet: 2.906 ± 0.57
5.968LysAsn: 5.968 ± 0.53
2.958LysPro: 2.958 ± 0.468
2.595LysGln: 2.595 ± 0.548
3.217LysArg: 3.217 ± 0.595
5.708LysSer: 5.708 ± 0.626
4.826LysThr: 4.826 ± 0.592
6.331LysVal: 6.331 ± 0.641
0.675LysTrp: 0.675 ± 0.162
4.67LysTyr: 4.67 ± 0.459
0.0LysXaa: 0.0 ± 0.0
Leu
4.099LeuAla: 4.099 ± 0.415
0.623LeuCys: 0.623 ± 0.254
5.708LeuAsp: 5.708 ± 0.553
5.916LeuGlu: 5.916 ± 0.662
3.477LeuPhe: 3.477 ± 0.386
4.099LeuGly: 4.099 ± 0.515
0.882LeuHis: 0.882 ± 0.162
5.5LeuIle: 5.5 ± 0.711
7.161LeuLys: 7.161 ± 0.774
6.59LeuLeu: 6.59 ± 0.6
1.972LeuMet: 1.972 ± 0.399
6.279LeuAsn: 6.279 ± 0.502
3.165LeuPro: 3.165 ± 0.341
2.698LeuGln: 2.698 ± 0.387
1.868LeuArg: 1.868 ± 0.372
6.435LeuSer: 6.435 ± 0.502
4.463LeuThr: 4.463 ± 0.506
3.788LeuVal: 3.788 ± 0.483
0.83LeuTrp: 0.83 ± 0.244
3.321LeuTyr: 3.321 ± 0.396
0.0LeuXaa: 0.0 ± 0.0
Met
2.179MetAla: 2.179 ± 0.318
0.208MetCys: 0.208 ± 0.108
0.675MetAsp: 0.675 ± 0.141
1.661MetGlu: 1.661 ± 0.261
0.83MetPhe: 0.83 ± 0.155
0.882MetGly: 0.882 ± 0.236
0.259MetHis: 0.259 ± 0.096
1.609MetIle: 1.609 ± 0.361
2.75MetLys: 2.75 ± 0.505
1.142MetLeu: 1.142 ± 0.244
0.571MetMet: 0.571 ± 0.216
2.076MetAsn: 2.076 ± 0.369
0.415MetPro: 0.415 ± 0.139
1.038MetGln: 1.038 ± 0.23
0.986MetArg: 0.986 ± 0.298
1.609MetSer: 1.609 ± 0.305
1.142MetThr: 1.142 ± 0.227
1.142MetVal: 1.142 ± 0.213
0.208MetTrp: 0.208 ± 0.103
1.349MetTyr: 1.349 ± 0.352
0.0MetXaa: 0.0 ± 0.0
Asn
4.463AsnAla: 4.463 ± 0.76
0.623AsnCys: 0.623 ± 0.198
4.411AsnAsp: 4.411 ± 0.57
4.618AsnGlu: 4.618 ± 0.485
3.113AsnPhe: 3.113 ± 0.424
4.982AsnGly: 4.982 ± 0.468
0.675AsnHis: 0.675 ± 0.18
5.033AsnIle: 5.033 ± 0.516
7.109AsnLys: 7.109 ± 0.73
4.566AsnLeu: 4.566 ± 0.726
1.816AsnMet: 1.816 ± 0.336
5.968AsnAsn: 5.968 ± 0.435
2.491AsnPro: 2.491 ± 0.406
3.165AsnGln: 3.165 ± 0.47
3.529AsnArg: 3.529 ± 0.467
4.515AsnSer: 4.515 ± 0.48
3.477AsnThr: 3.477 ± 0.474
4.307AsnVal: 4.307 ± 0.491
0.519AsnTrp: 0.519 ± 0.172
2.698AsnTyr: 2.698 ± 0.409
0.0AsnXaa: 0.0 ± 0.0
Pro
0.83ProAla: 0.83 ± 0.295
0.052ProCys: 0.052 ± 0.055
1.816ProAsp: 1.816 ± 0.397
2.128ProGlu: 2.128 ± 0.393
1.245ProPhe: 1.245 ± 0.315
1.349ProGly: 1.349 ± 0.233
0.208ProHis: 0.208 ± 0.111
2.179ProIle: 2.179 ± 0.291
1.661ProLys: 1.661 ± 0.386
2.076ProLeu: 2.076 ± 0.342
0.726ProMet: 0.726 ± 0.199
2.024ProAsn: 2.024 ± 0.344
0.675ProPro: 0.675 ± 0.189
0.726ProGln: 0.726 ± 0.22
1.297ProArg: 1.297 ± 0.243
2.802ProSer: 2.802 ± 0.427
2.543ProThr: 2.543 ± 0.404
1.557ProVal: 1.557 ± 0.221
0.052ProTrp: 0.052 ± 0.044
1.09ProTyr: 1.09 ± 0.217
0.0ProXaa: 0.0 ± 0.0
Gln
2.231GlnAla: 2.231 ± 0.369
0.0GlnCys: 0.0 ± 0.0
1.972GlnAsp: 1.972 ± 0.377
2.802GlnGlu: 2.802 ± 0.529
0.986GlnPhe: 0.986 ± 0.213
1.868GlnGly: 1.868 ± 0.437
0.259GlnHis: 0.259 ± 0.098
2.335GlnIle: 2.335 ± 0.349
2.854GlnLys: 2.854 ± 0.496
1.972GlnLeu: 1.972 ± 0.315
1.09GlnMet: 1.09 ± 0.249
1.661GlnAsn: 1.661 ± 0.221
0.726GlnPro: 0.726 ± 0.171
0.986GlnGln: 0.986 ± 0.257
1.09GlnArg: 1.09 ± 0.388
2.595GlnSer: 2.595 ± 0.368
1.297GlnThr: 1.297 ± 0.255
2.024GlnVal: 2.024 ± 0.228
0.415GlnTrp: 0.415 ± 0.15
1.401GlnTyr: 1.401 ± 0.206
0.0GlnXaa: 0.0 ± 0.0
Arg
1.92ArgAla: 1.92 ± 0.31
0.415ArgCys: 0.415 ± 0.19
2.387ArgAsp: 2.387 ± 0.416
2.335ArgGlu: 2.335 ± 0.367
1.505ArgPhe: 1.505 ± 0.231
2.698ArgGly: 2.698 ± 0.396
0.83ArgHis: 0.83 ± 0.304
2.75ArgIle: 2.75 ± 0.371
3.113ArgLys: 3.113 ± 0.461
3.425ArgLeu: 3.425 ± 0.389
0.778ArgMet: 0.778 ± 0.264
1.661ArgAsn: 1.661 ± 0.27
0.986ArgPro: 0.986 ± 0.221
0.83ArgGln: 0.83 ± 0.192
1.401ArgArg: 1.401 ± 0.357
1.816ArgSer: 1.816 ± 0.285
1.505ArgThr: 1.505 ± 0.325
2.231ArgVal: 2.231 ± 0.296
0.415ArgTrp: 0.415 ± 0.161
1.92ArgTyr: 1.92 ± 0.324
0.0ArgXaa: 0.0 ± 0.0
Ser
3.01SerAla: 3.01 ± 0.466
0.571SerCys: 0.571 ± 0.226
5.345SerAsp: 5.345 ± 0.559
5.293SerGlu: 5.293 ± 0.478
3.373SerPhe: 3.373 ± 0.479
5.137SerGly: 5.137 ± 0.494
0.415SerHis: 0.415 ± 0.157
5.812SerIle: 5.812 ± 0.509
7.369SerLys: 7.369 ± 0.893
5.397SerLeu: 5.397 ± 0.528
1.661SerMet: 1.661 ± 0.272
5.5SerAsn: 5.5 ± 0.425
1.816SerPro: 1.816 ± 0.333
1.661SerGln: 1.661 ± 0.201
2.387SerArg: 2.387 ± 0.348
5.033SerSer: 5.033 ± 0.566
3.321SerThr: 3.321 ± 0.426
4.774SerVal: 4.774 ± 0.55
0.623SerTrp: 0.623 ± 0.18
3.892SerTyr: 3.892 ± 0.424
0.0SerXaa: 0.0 ± 0.0
Thr
2.958ThrAla: 2.958 ± 0.453
0.208ThrCys: 0.208 ± 0.105
3.632ThrAsp: 3.632 ± 0.449
3.736ThrGlu: 3.736 ± 0.431
1.92ThrPhe: 1.92 ± 0.457
3.321ThrGly: 3.321 ± 0.424
0.623ThrHis: 0.623 ± 0.236
4.411ThrIle: 4.411 ± 0.641
5.864ThrLys: 5.864 ± 0.501
5.033ThrLeu: 5.033 ± 0.774
0.519ThrMet: 0.519 ± 0.184
3.84ThrAsn: 3.84 ± 0.539
3.062ThrPro: 3.062 ± 0.341
1.557ThrGln: 1.557 ± 0.267
1.661ThrArg: 1.661 ± 0.255
3.736ThrSer: 3.736 ± 0.37
4.982ThrThr: 4.982 ± 0.711
2.387ThrVal: 2.387 ± 0.529
0.571ThrTrp: 0.571 ± 0.156
2.802ThrTyr: 2.802 ± 0.386
0.0ThrXaa: 0.0 ± 0.0
Val
3.373ValAla: 3.373 ± 0.517
0.519ValCys: 0.519 ± 0.177
3.944ValAsp: 3.944 ± 0.388
4.774ValGlu: 4.774 ± 0.478
2.802ValPhe: 2.802 ± 0.368
3.892ValGly: 3.892 ± 0.452
0.467ValHis: 0.467 ± 0.185
4.878ValIle: 4.878 ± 0.564
5.656ValLys: 5.656 ± 0.68
4.93ValLeu: 4.93 ± 0.581
0.83ValMet: 0.83 ± 0.218
4.048ValAsn: 4.048 ± 0.501
1.609ValPro: 1.609 ± 0.32
1.505ValGln: 1.505 ± 0.203
2.024ValArg: 2.024 ± 0.323
4.566ValSer: 4.566 ± 0.581
3.062ValThr: 3.062 ± 0.625
3.736ValVal: 3.736 ± 0.463
0.467ValTrp: 0.467 ± 0.193
2.906ValTyr: 2.906 ± 0.473
0.0ValXaa: 0.0 ± 0.0
Trp
0.467TrpAla: 0.467 ± 0.133
0.052TrpCys: 0.052 ± 0.064
0.467TrpAsp: 0.467 ± 0.171
0.623TrpGlu: 0.623 ± 0.18
0.726TrpPhe: 0.726 ± 0.228
0.623TrpGly: 0.623 ± 0.195
0.052TrpHis: 0.052 ± 0.061
0.363TrpIle: 0.363 ± 0.138
0.675TrpLys: 0.675 ± 0.222
0.675TrpLeu: 0.675 ± 0.176
0.363TrpMet: 0.363 ± 0.149
0.726TrpAsn: 0.726 ± 0.173
0.0TrpPro: 0.0 ± 0.0
0.363TrpGln: 0.363 ± 0.15
0.623TrpArg: 0.623 ± 0.176
0.882TrpSer: 0.882 ± 0.249
0.363TrpThr: 0.363 ± 0.152
0.467TrpVal: 0.467 ± 0.182
0.052TrpTrp: 0.052 ± 0.056
0.311TrpTyr: 0.311 ± 0.138
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.491TyrAla: 2.491 ± 0.344
0.519TyrCys: 0.519 ± 0.186
3.269TyrAsp: 3.269 ± 0.448
2.854TyrGlu: 2.854 ± 0.418
2.387TyrPhe: 2.387 ± 0.534
2.543TyrGly: 2.543 ± 0.343
0.675TyrHis: 0.675 ± 0.178
3.529TyrIle: 3.529 ± 0.427
4.099TyrLys: 4.099 ± 0.397
3.684TyrLeu: 3.684 ± 0.454
0.986TyrMet: 0.986 ± 0.205
3.892TyrAsn: 3.892 ± 0.461
1.194TyrPro: 1.194 ± 0.219
1.453TyrGln: 1.453 ± 0.257
1.245TyrArg: 1.245 ± 0.235
3.062TyrSer: 3.062 ± 0.437
3.113TyrThr: 3.113 ± 0.505
2.595TyrVal: 2.595 ± 0.467
0.415TyrTrp: 0.415 ± 0.168
2.179TyrTyr: 2.179 ± 0.417
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 90 proteins (19272 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski