Amino acid dipepetide frequency for uncultured phage_MedDCM-OCT-S35-C6

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.245AlaAla: 3.245 ± 0.827
0.633AlaCys: 0.633 ± 0.242
4.986AlaAsp: 4.986 ± 0.504
5.54AlaGlu: 5.54 ± 0.836
3.007AlaPhe: 3.007 ± 0.587
5.223AlaGly: 5.223 ± 1.285
0.791AlaHis: 0.791 ± 0.265
5.144AlaIle: 5.144 ± 0.648
5.856AlaLys: 5.856 ± 0.831
5.461AlaLeu: 5.461 ± 0.771
2.612AlaMet: 2.612 ± 0.447
5.461AlaAsn: 5.461 ± 0.822
2.058AlaPro: 2.058 ± 0.436
2.849AlaGln: 2.849 ± 0.466
2.058AlaArg: 2.058 ± 0.465
4.986AlaSer: 4.986 ± 0.769
6.094AlaThr: 6.094 ± 1.203
3.878AlaVal: 3.878 ± 0.56
0.791AlaTrp: 0.791 ± 0.299
2.612AlaTyr: 2.612 ± 0.401
0.0AlaXaa: 0.0 ± 0.0
Cys
0.237CysAla: 0.237 ± 0.127
0.079CysCys: 0.079 ± 0.087
0.317CysAsp: 0.317 ± 0.185
0.396CysGlu: 0.396 ± 0.168
0.396CysPhe: 0.396 ± 0.164
0.475CysGly: 0.475 ± 0.235
0.237CysHis: 0.237 ± 0.129
0.237CysIle: 0.237 ± 0.145
0.554CysLys: 0.554 ± 0.189
0.95CysLeu: 0.95 ± 0.395
0.475CysMet: 0.475 ± 0.201
0.317CysAsn: 0.317 ± 0.217
0.158CysPro: 0.158 ± 0.106
0.237CysGln: 0.237 ± 0.159
0.475CysArg: 0.475 ± 0.196
0.633CysSer: 0.633 ± 0.261
0.554CysThr: 0.554 ± 0.189
0.237CysVal: 0.237 ± 0.12
0.237CysTrp: 0.237 ± 0.154
0.317CysTyr: 0.317 ± 0.141
0.0CysXaa: 0.0 ± 0.0
Asp
4.511AspAla: 4.511 ± 0.663
0.079AspCys: 0.079 ± 0.087
3.72AspAsp: 3.72 ± 0.523
3.086AspGlu: 3.086 ± 0.561
2.453AspPhe: 2.453 ± 0.493
5.144AspGly: 5.144 ± 0.678
0.554AspHis: 0.554 ± 0.225
4.669AspIle: 4.669 ± 0.408
4.827AspLys: 4.827 ± 0.938
6.015AspLeu: 6.015 ± 0.552
1.504AspMet: 1.504 ± 0.306
2.849AspAsn: 2.849 ± 0.549
1.662AspPro: 1.662 ± 0.348
1.345AspGln: 1.345 ± 0.353
3.086AspArg: 3.086 ± 0.735
3.561AspSer: 3.561 ± 0.572
3.878AspThr: 3.878 ± 0.628
3.245AspVal: 3.245 ± 0.496
0.95AspTrp: 0.95 ± 0.328
2.374AspTyr: 2.374 ± 0.322
0.0AspXaa: 0.0 ± 0.0
Glu
4.511GluAla: 4.511 ± 0.612
0.554GluCys: 0.554 ± 0.229
3.561GluAsp: 3.561 ± 0.498
4.036GluGlu: 4.036 ± 0.804
2.137GluPhe: 2.137 ± 0.492
3.799GluGly: 3.799 ± 0.49
1.029GluHis: 1.029 ± 0.331
5.144GluIle: 5.144 ± 0.678
6.173GluLys: 6.173 ± 1.052
4.59GluLeu: 4.59 ± 0.652
1.899GluMet: 1.899 ± 0.523
3.64GluAsn: 3.64 ± 0.532
1.425GluPro: 1.425 ± 0.444
2.849GluGln: 2.849 ± 0.788
3.086GluArg: 3.086 ± 0.729
2.532GluSer: 2.532 ± 0.388
4.036GluThr: 4.036 ± 0.699
4.036GluVal: 4.036 ± 0.404
0.871GluTrp: 0.871 ± 0.357
2.77GluTyr: 2.77 ± 0.522
0.0GluXaa: 0.0 ± 0.0
Phe
2.453PheAla: 2.453 ± 0.444
0.396PheCys: 0.396 ± 0.236
2.77PheAsp: 2.77 ± 0.437
2.137PheGlu: 2.137 ± 0.423
1.266PhePhe: 1.266 ± 0.329
2.849PheGly: 2.849 ± 0.615
0.712PheHis: 0.712 ± 0.279
2.532PheIle: 2.532 ± 0.336
3.878PheLys: 3.878 ± 0.592
2.691PheLeu: 2.691 ± 0.584
1.504PheMet: 1.504 ± 0.313
2.058PheAsn: 2.058 ± 0.383
0.475PhePro: 0.475 ± 0.189
1.345PheGln: 1.345 ± 0.357
1.425PheArg: 1.425 ± 0.29
2.058PheSer: 2.058 ± 0.502
3.403PheThr: 3.403 ± 0.704
2.612PheVal: 2.612 ± 0.421
0.712PheTrp: 0.712 ± 0.256
1.425PheTyr: 1.425 ± 0.329
0.0PheXaa: 0.0 ± 0.0
Gly
4.036GlyAla: 4.036 ± 0.868
0.317GlyCys: 0.317 ± 0.185
4.036GlyAsp: 4.036 ± 0.471
3.324GlyGlu: 3.324 ± 0.574
3.403GlyPhe: 3.403 ± 0.486
4.353GlyGly: 4.353 ± 0.635
1.345GlyHis: 1.345 ± 0.34
4.353GlyIle: 4.353 ± 0.56
4.59GlyLys: 4.59 ± 0.628
4.827GlyLeu: 4.827 ± 0.607
1.583GlyMet: 1.583 ± 0.363
4.511GlyAsn: 4.511 ± 0.607
0.0GlyPro: 0.0 ± 0.0
2.612GlyGln: 2.612 ± 0.665
2.295GlyArg: 2.295 ± 0.429
6.331GlySer: 6.331 ± 1.325
5.065GlyThr: 5.065 ± 0.853
4.511GlyVal: 4.511 ± 0.606
0.712GlyTrp: 0.712 ± 0.21
2.928GlyTyr: 2.928 ± 0.433
0.0GlyXaa: 0.0 ± 0.0
His
2.058HisAla: 2.058 ± 0.402
0.158HisCys: 0.158 ± 0.104
0.871HisAsp: 0.871 ± 0.293
0.712HisGlu: 0.712 ± 0.289
1.029HisPhe: 1.029 ± 0.221
0.791HisGly: 0.791 ± 0.251
0.237HisHis: 0.237 ± 0.189
1.266HisIle: 1.266 ± 0.417
0.633HisLys: 0.633 ± 0.254
1.82HisLeu: 1.82 ± 0.424
0.475HisMet: 0.475 ± 0.217
0.95HisAsn: 0.95 ± 0.26
0.475HisPro: 0.475 ± 0.162
0.554HisGln: 0.554 ± 0.212
0.633HisArg: 0.633 ± 0.195
1.345HisSer: 1.345 ± 0.28
1.345HisThr: 1.345 ± 0.259
0.95HisVal: 0.95 ± 0.267
0.475HisTrp: 0.475 ± 0.181
0.554HisTyr: 0.554 ± 0.2
0.0HisXaa: 0.0 ± 0.0
Ile
5.302IleAla: 5.302 ± 0.498
0.317IleCys: 0.317 ± 0.152
4.827IleAsp: 4.827 ± 0.536
4.353IleGlu: 4.353 ± 0.775
1.82IlePhe: 1.82 ± 0.482
3.957IleGly: 3.957 ± 0.557
1.187IleHis: 1.187 ± 0.278
4.115IleIle: 4.115 ± 0.678
6.41IleLys: 6.41 ± 0.691
4.511IleLeu: 4.511 ± 0.564
1.978IleMet: 1.978 ± 0.395
4.115IleAsn: 4.115 ± 0.635
2.849IlePro: 2.849 ± 0.469
1.741IleGln: 1.741 ± 0.271
2.612IleArg: 2.612 ± 0.389
4.827IleSer: 4.827 ± 0.725
5.935IleThr: 5.935 ± 0.929
3.561IleVal: 3.561 ± 0.501
0.475IleTrp: 0.475 ± 0.18
1.583IleTyr: 1.583 ± 0.342
0.0IleXaa: 0.0 ± 0.0
Lys
4.827LysAla: 4.827 ± 0.791
0.712LysCys: 0.712 ± 0.283
3.957LysAsp: 3.957 ± 0.621
7.36LysGlu: 7.36 ± 0.963
2.849LysPhe: 2.849 ± 0.475
3.878LysGly: 3.878 ± 0.616
1.583LysHis: 1.583 ± 0.403
6.015LysIle: 6.015 ± 0.647
7.043LysLys: 7.043 ± 1.092
7.043LysLeu: 7.043 ± 0.676
2.216LysMet: 2.216 ± 0.491
4.669LysAsn: 4.669 ± 0.702
2.849LysPro: 2.849 ± 0.508
2.532LysGln: 2.532 ± 0.496
4.036LysArg: 4.036 ± 0.757
6.648LysSer: 6.648 ± 0.853
5.223LysThr: 5.223 ± 0.758
4.194LysVal: 4.194 ± 0.756
1.029LysTrp: 1.029 ± 0.318
3.086LysTyr: 3.086 ± 0.588
0.0LysXaa: 0.0 ± 0.0
Leu
5.223LeuAla: 5.223 ± 0.686
0.475LeuCys: 0.475 ± 0.236
5.856LeuAsp: 5.856 ± 0.7
5.302LeuGlu: 5.302 ± 0.88
2.612LeuPhe: 2.612 ± 0.45
3.878LeuGly: 3.878 ± 0.568
1.82LeuHis: 1.82 ± 0.455
4.115LeuIle: 4.115 ± 0.585
6.331LeuLys: 6.331 ± 0.546
6.569LeuLeu: 6.569 ± 0.865
1.899LeuMet: 1.899 ± 0.363
5.461LeuAsn: 5.461 ± 0.543
3.403LeuPro: 3.403 ± 0.442
3.64LeuGln: 3.64 ± 0.586
3.086LeuArg: 3.086 ± 0.491
5.381LeuSer: 5.381 ± 0.591
5.698LeuThr: 5.698 ± 0.951
4.748LeuVal: 4.748 ± 0.485
1.029LeuTrp: 1.029 ± 0.361
2.216LeuTyr: 2.216 ± 0.351
0.0LeuXaa: 0.0 ± 0.0
Met
3.166MetAla: 3.166 ± 0.356
0.396MetCys: 0.396 ± 0.198
1.187MetAsp: 1.187 ± 0.281
1.266MetGlu: 1.266 ± 0.373
0.95MetPhe: 0.95 ± 0.251
1.741MetGly: 1.741 ± 0.405
0.317MetHis: 0.317 ± 0.164
1.187MetIle: 1.187 ± 0.288
2.691MetLys: 2.691 ± 0.641
2.058MetLeu: 2.058 ± 0.494
0.95MetMet: 0.95 ± 0.293
1.029MetAsn: 1.029 ± 0.279
1.583MetPro: 1.583 ± 0.414
1.266MetGln: 1.266 ± 0.272
1.187MetArg: 1.187 ± 0.324
2.77MetSer: 2.77 ± 0.406
1.029MetThr: 1.029 ± 0.306
1.425MetVal: 1.425 ± 0.34
0.396MetTrp: 0.396 ± 0.161
0.95MetTyr: 0.95 ± 0.295
0.0MetXaa: 0.0 ± 0.0
Asn
5.223AsnAla: 5.223 ± 0.765
0.554AsnCys: 0.554 ± 0.171
3.324AsnAsp: 3.324 ± 0.598
3.482AsnGlu: 3.482 ± 0.417
2.928AsnPhe: 2.928 ± 0.488
4.907AsnGly: 4.907 ± 0.703
0.712AsnHis: 0.712 ± 0.181
4.748AsnIle: 4.748 ± 0.824
3.799AsnLys: 3.799 ± 0.476
5.065AsnLeu: 5.065 ± 0.484
1.82AsnMet: 1.82 ± 0.442
4.353AsnAsn: 4.353 ± 0.651
1.82AsnPro: 1.82 ± 0.36
2.058AsnGln: 2.058 ± 0.35
3.482AsnArg: 3.482 ± 0.546
4.986AsnSer: 4.986 ± 0.967
5.065AsnThr: 5.065 ± 0.973
2.77AsnVal: 2.77 ± 0.487
0.475AsnTrp: 0.475 ± 0.177
2.849AsnTyr: 2.849 ± 0.406
0.0AsnXaa: 0.0 ± 0.0
Pro
1.662ProAla: 1.662 ± 0.269
0.158ProCys: 0.158 ± 0.112
1.583ProAsp: 1.583 ± 0.353
2.928ProGlu: 2.928 ± 0.654
1.504ProPhe: 1.504 ± 0.304
0.0ProGly: 0.0 ± 0.0
0.554ProHis: 0.554 ± 0.155
2.058ProIle: 2.058 ± 0.461
2.77ProLys: 2.77 ± 0.551
2.532ProLeu: 2.532 ± 0.407
0.712ProMet: 0.712 ± 0.269
1.899ProAsn: 1.899 ± 0.376
1.583ProPro: 1.583 ± 0.388
1.187ProGln: 1.187 ± 0.331
0.317ProArg: 0.317 ± 0.145
2.849ProSer: 2.849 ± 0.356
2.612ProThr: 2.612 ± 0.395
1.662ProVal: 1.662 ± 0.42
0.0ProTrp: 0.0 ± 0.0
0.871ProTyr: 0.871 ± 0.285
0.0ProXaa: 0.0 ± 0.0
Gln
2.849GlnAla: 2.849 ± 0.568
0.158GlnCys: 0.158 ± 0.115
2.216GlnAsp: 2.216 ± 0.4
2.849GlnGlu: 2.849 ± 0.41
1.741GlnPhe: 1.741 ± 0.421
1.266GlnGly: 1.266 ± 0.254
0.475GlnHis: 0.475 ± 0.214
1.899GlnIle: 1.899 ± 0.415
3.245GlnLys: 3.245 ± 0.485
3.64GlnLeu: 3.64 ± 0.64
1.187GlnMet: 1.187 ± 0.45
1.978GlnAsn: 1.978 ± 0.401
1.029GlnPro: 1.029 ± 0.247
1.425GlnGln: 1.425 ± 0.352
1.345GlnArg: 1.345 ± 0.317
2.928GlnSer: 2.928 ± 0.381
3.086GlnThr: 3.086 ± 0.568
1.978GlnVal: 1.978 ± 0.471
0.396GlnTrp: 0.396 ± 0.163
1.266GlnTyr: 1.266 ± 0.296
0.0GlnXaa: 0.0 ± 0.0
Arg
2.928ArgAla: 2.928 ± 0.458
0.0ArgCys: 0.0 ± 0.0
2.691ArgAsp: 2.691 ± 0.46
2.453ArgGlu: 2.453 ± 0.516
1.029ArgPhe: 1.029 ± 0.274
2.532ArgGly: 2.532 ± 0.463
0.712ArgHis: 0.712 ± 0.286
2.216ArgIle: 2.216 ± 0.399
3.878ArgLys: 3.878 ± 0.621
3.086ArgLeu: 3.086 ± 0.639
1.662ArgMet: 1.662 ± 0.505
2.216ArgAsn: 2.216 ± 0.428
0.871ArgPro: 0.871 ± 0.221
1.425ArgGln: 1.425 ± 0.323
1.662ArgArg: 1.662 ± 0.323
2.295ArgSer: 2.295 ± 0.444
1.583ArgThr: 1.583 ± 0.331
1.741ArgVal: 1.741 ± 0.453
0.633ArgTrp: 0.633 ± 0.272
2.058ArgTyr: 2.058 ± 0.467
0.0ArgXaa: 0.0 ± 0.0
Ser
6.015SerAla: 6.015 ± 0.92
0.475SerCys: 0.475 ± 0.197
3.72SerAsp: 3.72 ± 0.565
4.115SerGlu: 4.115 ± 0.505
3.086SerPhe: 3.086 ± 0.651
6.806SerGly: 6.806 ± 1.136
1.583SerHis: 1.583 ± 0.288
5.144SerIle: 5.144 ± 0.579
5.461SerLys: 5.461 ± 0.524
5.065SerLeu: 5.065 ± 0.648
1.266SerMet: 1.266 ± 0.284
5.381SerAsn: 5.381 ± 0.906
2.058SerPro: 2.058 ± 0.344
3.086SerGln: 3.086 ± 0.471
1.662SerArg: 1.662 ± 0.304
6.173SerSer: 6.173 ± 1.199
5.698SerThr: 5.698 ± 1.141
4.115SerVal: 4.115 ± 0.705
1.425SerTrp: 1.425 ± 0.258
2.453SerTyr: 2.453 ± 0.42
0.0SerXaa: 0.0 ± 0.0
Thr
6.806ThrAla: 6.806 ± 0.81
0.475ThrCys: 0.475 ± 0.267
3.878ThrAsp: 3.878 ± 0.795
3.561ThrGlu: 3.561 ± 0.515
2.532ThrPhe: 2.532 ± 0.645
6.331ThrGly: 6.331 ± 0.845
1.425ThrHis: 1.425 ± 0.274
4.827ThrIle: 4.827 ± 0.557
4.432ThrLys: 4.432 ± 0.526
5.698ThrLeu: 5.698 ± 0.842
1.425ThrMet: 1.425 ± 0.285
6.015ThrAsn: 6.015 ± 1.21
2.691ThrPro: 2.691 ± 0.467
2.453ThrGln: 2.453 ± 0.514
1.583ThrArg: 1.583 ± 0.311
6.094ThrSer: 6.094 ± 1.127
6.489ThrThr: 6.489 ± 1.48
4.432ThrVal: 4.432 ± 0.908
0.554ThrTrp: 0.554 ± 0.189
2.532ThrTyr: 2.532 ± 0.492
0.0ThrXaa: 0.0 ± 0.0
Val
5.698ValAla: 5.698 ± 1.125
0.791ValCys: 0.791 ± 0.291
3.245ValAsp: 3.245 ± 0.437
3.64ValGlu: 3.64 ± 0.605
2.216ValPhe: 2.216 ± 0.379
4.432ValGly: 4.432 ± 0.424
1.108ValHis: 1.108 ± 0.477
3.245ValIle: 3.245 ± 0.556
4.669ValLys: 4.669 ± 0.645
2.532ValLeu: 2.532 ± 0.573
1.425ValMet: 1.425 ± 0.349
4.274ValAsn: 4.274 ± 0.626
1.899ValPro: 1.899 ± 0.362
1.741ValGln: 1.741 ± 0.318
2.295ValArg: 2.295 ± 0.331
4.511ValSer: 4.511 ± 0.663
3.878ValThr: 3.878 ± 0.523
2.374ValVal: 2.374 ± 0.401
0.475ValTrp: 0.475 ± 0.184
1.266ValTyr: 1.266 ± 0.306
0.0ValXaa: 0.0 ± 0.0
Trp
0.712TrpAla: 0.712 ± 0.227
0.317TrpCys: 0.317 ± 0.227
1.029TrpAsp: 1.029 ± 0.279
0.396TrpGlu: 0.396 ± 0.169
0.237TrpPhe: 0.237 ± 0.153
0.475TrpGly: 0.475 ± 0.225
0.158TrpHis: 0.158 ± 0.14
1.108TrpIle: 1.108 ± 0.351
1.029TrpLys: 1.029 ± 0.265
1.583TrpLeu: 1.583 ± 0.358
0.079TrpMet: 0.079 ± 0.075
0.95TrpAsn: 0.95 ± 0.208
0.0TrpPro: 0.0 ± 0.0
0.871TrpGln: 0.871 ± 0.243
0.712TrpArg: 0.712 ± 0.229
1.029TrpSer: 1.029 ± 0.266
1.108TrpThr: 1.108 ± 0.268
0.475TrpVal: 0.475 ± 0.192
0.079TrpTrp: 0.079 ± 0.081
0.158TrpTyr: 0.158 ± 0.171
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.82TyrAla: 1.82 ± 0.324
0.475TyrCys: 0.475 ± 0.22
1.741TyrAsp: 1.741 ± 0.331
1.741TyrGlu: 1.741 ± 0.402
1.504TyrPhe: 1.504 ± 0.414
2.295TyrGly: 2.295 ± 0.494
0.791TyrHis: 0.791 ± 0.236
2.453TyrIle: 2.453 ± 0.467
3.482TyrLys: 3.482 ± 0.596
3.007TyrLeu: 3.007 ± 0.483
0.791TyrMet: 0.791 ± 0.239
2.295TyrAsn: 2.295 ± 0.399
0.554TyrPro: 0.554 ± 0.207
1.741TyrGln: 1.741 ± 0.344
0.633TyrArg: 0.633 ± 0.211
2.849TyrSer: 2.849 ± 0.363
2.453TyrThr: 2.453 ± 0.451
2.77TyrVal: 2.77 ± 0.476
0.791TyrTrp: 0.791 ± 0.259
1.425TyrTyr: 1.425 ± 0.364
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (12637 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski