Natrarchaeobius halalkaliphilus

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Natrialbales; Natrialbaceae; Natrarchaeobius

Average proteome isoelectric point is 4.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3330 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N6NWK0|A0A3N6NWK0_9EURY ArsR family transcriptional regulator OS=Natrarchaeobius halalkaliphilus OX=1679091 GN=EA462_11025 PE=4 SV=1
MM1 pKa = 7.41KK2 pKa = 10.4RR3 pKa = 11.84LLLIVLVAAMLVLAGCADD21 pKa = 3.81GAITEE26 pKa = 4.61DD27 pKa = 4.38DD28 pKa = 3.99GTTVEE33 pKa = 4.9ADD35 pKa = 4.31DD36 pKa = 4.41NFEE39 pKa = 4.94DD40 pKa = 5.4ADD42 pKa = 4.72SNDD45 pKa = 3.87ADD47 pKa = 5.76DD48 pKa = 5.76DD49 pKa = 4.21TTTDD53 pKa = 4.02DD54 pKa = 5.78DD55 pKa = 4.01EE56 pKa = 5.19TDD58 pKa = 3.42TEE60 pKa = 4.39EE61 pKa = 4.29TGEE64 pKa = 4.26SEE66 pKa = 5.1AGEE69 pKa = 4.62PDD71 pKa = 3.81PGDD74 pKa = 3.78PDD76 pKa = 3.23VDD78 pKa = 3.87GEE80 pKa = 4.69LEE82 pKa = 3.78IHH84 pKa = 6.89HH85 pKa = 7.0IDD87 pKa = 4.14VGQADD92 pKa = 4.2ATLLIEE98 pKa = 4.52PSGEE102 pKa = 4.13TILIDD107 pKa = 4.32SGDD110 pKa = 3.44WRR112 pKa = 11.84QGGSDD117 pKa = 3.63VIEE120 pKa = 4.0YY121 pKa = 10.79LGDD124 pKa = 3.51QDD126 pKa = 4.63IDD128 pKa = 4.7RR129 pKa = 11.84IDD131 pKa = 3.85HH132 pKa = 6.38LVATHH137 pKa = 5.68GHH139 pKa = 6.58ADD141 pKa = 3.61HH142 pKa = 7.2IGGHH146 pKa = 5.85DD147 pKa = 4.36AIIEE151 pKa = 4.25HH152 pKa = 6.64YY153 pKa = 8.04EE154 pKa = 3.97TEE156 pKa = 3.92RR157 pKa = 11.84DD158 pKa = 4.24GIGVAYY164 pKa = 10.25DD165 pKa = 3.04SGIAHH170 pKa = 7.16TSQTYY175 pKa = 9.16EE176 pKa = 3.99RR177 pKa = 11.84YY178 pKa = 9.62LDD180 pKa = 4.42AIEE183 pKa = 4.12EE184 pKa = 4.31HH185 pKa = 7.31DD186 pKa = 4.08VEE188 pKa = 4.5LLVVEE193 pKa = 4.93EE194 pKa = 4.48GDD196 pKa = 3.51HH197 pKa = 7.81VEE199 pKa = 4.99FGDD202 pKa = 3.87ATVDD206 pKa = 3.61VLNPPAGNSGSDD218 pKa = 2.92LHH220 pKa = 6.85YY221 pKa = 11.17NSVALSIEE229 pKa = 4.09FGEE232 pKa = 4.43FSYY235 pKa = 9.83LTTGDD240 pKa = 3.85AEE242 pKa = 4.62ADD244 pKa = 3.21AEE246 pKa = 4.0QRR248 pKa = 11.84MVDD251 pKa = 3.34EE252 pKa = 4.7HH253 pKa = 7.38GEE255 pKa = 4.04RR256 pKa = 11.84LEE258 pKa = 4.06TDD260 pKa = 3.71AYY262 pKa = 10.19QAGHH266 pKa = 6.66HH267 pKa = 6.49GSTTSSTTPFMDD279 pKa = 2.93QATPEE284 pKa = 4.01VAIISSAYY292 pKa = 9.65DD293 pKa = 3.16SQYY296 pKa = 11.13GHH298 pKa = 6.95PHH300 pKa = 6.9DD301 pKa = 5.27EE302 pKa = 4.19VLEE305 pKa = 4.76DD306 pKa = 3.61YY307 pKa = 10.99ADD309 pKa = 4.12RR310 pKa = 11.84GIEE313 pKa = 4.25TYY315 pKa = 7.55WTAVHH320 pKa = 6.79GDD322 pKa = 3.38VALTTDD328 pKa = 3.47GSDD331 pKa = 3.29VEE333 pKa = 5.54LEE335 pKa = 4.37TEE337 pKa = 4.56HH338 pKa = 6.94EE339 pKa = 4.46FSTDD343 pKa = 3.13AGDD346 pKa = 4.21LLEE349 pKa = 5.37EE350 pKa = 4.89KK351 pKa = 10.27PADD354 pKa = 4.89DD355 pKa = 6.49DD356 pKa = 3.9DD357 pKa = 4.41TQASLTHH364 pKa = 6.94PIDD367 pKa = 3.61VATTPLAGG375 pKa = 3.6

Molecular weight: 40.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N6LQ48|A0A3N6LQ48_9EURY Acyl-CoA carboxylase subunit beta OS=Natrarchaeobius halalkaliphilus OX=1679091 GN=EA462_03600 PE=4 SV=1
MM1 pKa = 7.14VLQRR5 pKa = 11.84FLGSKK10 pKa = 8.46ATRR13 pKa = 11.84SLTVVSVLMEE23 pKa = 4.67AKK25 pKa = 10.07RR26 pKa = 11.84ALARR30 pKa = 11.84GRR32 pKa = 11.84RR33 pKa = 11.84TRR35 pKa = 11.84GLVLLGVAVVAWKK48 pKa = 8.04WTVIGLAAQGVIKK61 pKa = 10.39ALRR64 pKa = 11.84AGRR67 pKa = 11.84PSGSSSAA74 pKa = 3.79

Molecular weight: 7.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3330

0

3330

976133

32

1921

293.1

32.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.759 ± 0.057

0.761 ± 0.014

8.587 ± 0.066

9.119 ± 0.057

3.27 ± 0.031

8.196 ± 0.04

2.053 ± 0.018

4.775 ± 0.034

1.821 ± 0.025

8.727 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.75 ± 0.021

2.456 ± 0.022

4.525 ± 0.024

2.383 ± 0.024

6.686 ± 0.049

6.037 ± 0.035

6.548 ± 0.031

8.76 ± 0.042

1.085 ± 0.017

2.702 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski