Amino acid dipepetide frequency for Cyanophage S-RIM4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.615AlaAla: 6.615 ± 0.578
0.521AlaCys: 0.521 ± 0.126
4.35AlaAsp: 4.35 ± 0.328
4.242AlaGlu: 4.242 ± 0.336
2.642AlaPhe: 2.642 ± 0.229
6.453AlaGly: 6.453 ± 0.498
0.953AlaHis: 0.953 ± 0.137
3.811AlaIle: 3.811 ± 0.292
4.278AlaLys: 4.278 ± 0.405
4.925AlaLeu: 4.925 ± 0.42
1.528AlaMet: 1.528 ± 0.224
4.026AlaAsn: 4.026 ± 0.324
2.822AlaPro: 2.822 ± 0.259
2.66AlaGln: 2.66 ± 0.225
2.786AlaArg: 2.786 ± 0.228
5.446AlaSer: 5.446 ± 0.491
5.68AlaThr: 5.68 ± 0.413
4.26AlaVal: 4.26 ± 0.274
0.737AlaTrp: 0.737 ± 0.113
2.337AlaTyr: 2.337 ± 0.224
0.0AlaXaa: 0.0 ± 0.0
Cys
0.647CysAla: 0.647 ± 0.092
0.054CysCys: 0.054 ± 0.034
0.755CysAsp: 0.755 ± 0.155
0.647CysGlu: 0.647 ± 0.14
0.557CysPhe: 0.557 ± 0.12
0.629CysGly: 0.629 ± 0.139
0.216CysHis: 0.216 ± 0.068
0.665CysIle: 0.665 ± 0.116
0.683CysLys: 0.683 ± 0.125
0.593CysLeu: 0.593 ± 0.106
0.306CysMet: 0.306 ± 0.081
0.467CysAsn: 0.467 ± 0.098
0.395CysPro: 0.395 ± 0.102
0.252CysGln: 0.252 ± 0.076
0.431CysArg: 0.431 ± 0.096
0.557CysSer: 0.557 ± 0.118
0.485CysThr: 0.485 ± 0.111
0.629CysVal: 0.629 ± 0.109
0.072CysTrp: 0.072 ± 0.036
0.395CysTyr: 0.395 ± 0.077
0.0CysXaa: 0.0 ± 0.0
Asp
5.087AspAla: 5.087 ± 0.377
0.881AspCys: 0.881 ± 0.145
4.602AspAsp: 4.602 ± 0.331
4.098AspGlu: 4.098 ± 0.33
3.415AspPhe: 3.415 ± 0.251
5.68AspGly: 5.68 ± 0.553
0.935AspHis: 0.935 ± 0.159
3.883AspIle: 3.883 ± 0.259
3.343AspLys: 3.343 ± 0.333
5.41AspLeu: 5.41 ± 0.35
1.348AspMet: 1.348 ± 0.183
3.361AspAsn: 3.361 ± 0.235
3.523AspPro: 3.523 ± 0.24
2.427AspGln: 2.427 ± 0.208
2.822AspArg: 2.822 ± 0.281
3.883AspSer: 3.883 ± 0.311
4.368AspThr: 4.368 ± 0.328
4.332AspVal: 4.332 ± 0.295
1.078AspTrp: 1.078 ± 0.143
3.182AspTyr: 3.182 ± 0.25
0.0AspXaa: 0.0 ± 0.0
Glu
3.397GluAla: 3.397 ± 0.276
0.575GluCys: 0.575 ± 0.115
4.152GluAsp: 4.152 ± 0.306
5.392GluGlu: 5.392 ± 0.655
3.092GluPhe: 3.092 ± 0.255
3.9GluGly: 3.9 ± 0.292
0.899GluHis: 0.899 ± 0.152
4.134GluIle: 4.134 ± 0.269
4.026GluLys: 4.026 ± 0.381
4.35GluLeu: 4.35 ± 0.334
1.905GluMet: 1.905 ± 0.266
3.397GluAsn: 3.397 ± 0.22
1.366GluPro: 1.366 ± 0.151
2.013GluGln: 2.013 ± 0.235
2.463GluArg: 2.463 ± 0.232
3.954GluSer: 3.954 ± 0.399
4.17GluThr: 4.17 ± 0.341
4.745GluVal: 4.745 ± 0.302
0.917GluTrp: 0.917 ± 0.124
2.822GluTyr: 2.822 ± 0.227
0.0GluXaa: 0.0 ± 0.0
Phe
2.606PheAla: 2.606 ± 0.218
0.467PheCys: 0.467 ± 0.102
3.721PheAsp: 3.721 ± 0.223
2.606PheGlu: 2.606 ± 0.216
2.013PhePhe: 2.013 ± 0.197
3.11PheGly: 3.11 ± 0.25
0.629PheHis: 0.629 ± 0.128
2.642PheIle: 2.642 ± 0.223
2.265PheLys: 2.265 ± 0.219
3.235PheLeu: 3.235 ± 0.304
1.222PheMet: 1.222 ± 0.169
2.732PheAsn: 2.732 ± 0.268
1.564PhePro: 1.564 ± 0.186
1.6PheGln: 1.6 ± 0.158
1.708PheArg: 1.708 ± 0.157
3.146PheSer: 3.146 ± 0.243
3.667PheThr: 3.667 ± 0.249
2.858PheVal: 2.858 ± 0.268
0.467PheTrp: 0.467 ± 0.109
1.779PheTyr: 1.779 ± 0.182
0.0PheXaa: 0.0 ± 0.0
Gly
5.968GlyAla: 5.968 ± 0.528
0.683GlyCys: 0.683 ± 0.123
4.853GlyAsp: 4.853 ± 0.442
3.685GlyGlu: 3.685 ± 0.275
3.056GlyPhe: 3.056 ± 0.232
7.531GlyGly: 7.531 ± 0.74
0.953GlyHis: 0.953 ± 0.144
4.422GlyIle: 4.422 ± 0.337
3.775GlyLys: 3.775 ± 0.403
4.44GlyLeu: 4.44 ± 0.317
1.618GlyMet: 1.618 ± 0.223
4.53GlyAsn: 4.53 ± 0.429
2.103GlyPro: 2.103 ± 0.215
2.66GlyGln: 2.66 ± 0.231
3.146GlyArg: 3.146 ± 0.232
5.932GlySer: 5.932 ± 0.609
6.021GlyThr: 6.021 ± 0.469
5.464GlyVal: 5.464 ± 0.357
1.096GlyTrp: 1.096 ± 0.136
3.379GlyTyr: 3.379 ± 0.406
0.0GlyXaa: 0.0 ± 0.0
His
0.719HisAla: 0.719 ± 0.12
0.198HisCys: 0.198 ± 0.059
0.755HisAsp: 0.755 ± 0.139
1.007HisGlu: 1.007 ± 0.185
0.809HisPhe: 0.809 ± 0.151
1.007HisGly: 1.007 ± 0.137
0.413HisHis: 0.413 ± 0.129
0.953HisIle: 0.953 ± 0.167
0.755HisLys: 0.755 ± 0.153
1.15HisLeu: 1.15 ± 0.178
0.377HisMet: 0.377 ± 0.086
0.665HisAsn: 0.665 ± 0.133
0.989HisPro: 0.989 ± 0.152
0.449HisGln: 0.449 ± 0.095
0.665HisArg: 0.665 ± 0.137
0.809HisSer: 0.809 ± 0.127
0.917HisThr: 0.917 ± 0.153
0.809HisVal: 0.809 ± 0.137
0.306HisTrp: 0.306 ± 0.077
0.719HisTyr: 0.719 ± 0.149
0.0HisXaa: 0.0 ± 0.0
Ile
4.206IleAla: 4.206 ± 0.335
0.575IleCys: 0.575 ± 0.105
5.177IleAsp: 5.177 ± 0.359
4.566IleGlu: 4.566 ± 0.29
2.516IlePhe: 2.516 ± 0.223
3.793IleGly: 3.793 ± 0.282
0.683IleHis: 0.683 ± 0.131
3.739IleIle: 3.739 ± 0.247
3.595IleLys: 3.595 ± 0.264
4.512IleLeu: 4.512 ± 0.28
0.899IleMet: 0.899 ± 0.132
4.566IleAsn: 4.566 ± 0.273
2.876IlePro: 2.876 ± 0.237
2.696IleGln: 2.696 ± 0.267
2.427IleArg: 2.427 ± 0.203
4.296IleSer: 4.296 ± 0.433
5.482IleThr: 5.482 ± 0.517
3.631IleVal: 3.631 ± 0.297
0.647IleTrp: 0.647 ± 0.133
2.229IleTyr: 2.229 ± 0.222
0.0IleXaa: 0.0 ± 0.0
Lys
3.307LysAla: 3.307 ± 0.323
0.737LysCys: 0.737 ± 0.14
3.595LysAsp: 3.595 ± 0.322
3.936LysGlu: 3.936 ± 0.449
2.427LysPhe: 2.427 ± 0.258
3.11LysGly: 3.11 ± 0.251
0.935LysHis: 0.935 ± 0.167
4.044LysIle: 4.044 ± 0.293
4.817LysLys: 4.817 ± 0.611
4.53LysLeu: 4.53 ± 0.318
1.546LysMet: 1.546 ± 0.233
2.984LysAsn: 2.984 ± 0.339
2.229LysPro: 2.229 ± 0.275
2.606LysGln: 2.606 ± 0.337
2.498LysArg: 2.498 ± 0.256
4.152LysSer: 4.152 ± 0.314
3.559LysThr: 3.559 ± 0.291
3.936LysVal: 3.936 ± 0.276
0.719LysTrp: 0.719 ± 0.128
2.858LysTyr: 2.858 ± 0.351
0.0LysXaa: 0.0 ± 0.0
Leu
5.41LeuAla: 5.41 ± 0.306
0.683LeuCys: 0.683 ± 0.145
4.997LeuAsp: 4.997 ± 0.326
4.655LeuGlu: 4.655 ± 0.264
2.714LeuPhe: 2.714 ± 0.228
4.691LeuGly: 4.691 ± 0.428
1.078LeuHis: 1.078 ± 0.188
4.206LeuIle: 4.206 ± 0.264
5.015LeuLys: 5.015 ± 0.464
5.446LeuLeu: 5.446 ± 0.368
1.438LeuMet: 1.438 ± 0.252
4.673LeuAsn: 4.673 ± 0.286
3.074LeuPro: 3.074 ± 0.305
2.768LeuGln: 2.768 ± 0.212
2.876LeuArg: 2.876 ± 0.231
4.619LeuSer: 4.619 ± 0.316
5.734LeuThr: 5.734 ± 0.392
4.673LeuVal: 4.673 ± 0.272
0.647LeuTrp: 0.647 ± 0.145
3.595LeuTyr: 3.595 ± 0.228
0.0LeuXaa: 0.0 ± 0.0
Met
1.582MetAla: 1.582 ± 0.221
0.234MetCys: 0.234 ± 0.075
1.24MetAsp: 1.24 ± 0.2
1.456MetGlu: 1.456 ± 0.24
0.737MetPhe: 0.737 ± 0.135
1.42MetGly: 1.42 ± 0.232
0.485MetHis: 0.485 ± 0.11
1.222MetIle: 1.222 ± 0.151
1.51MetLys: 1.51 ± 0.252
1.708MetLeu: 1.708 ± 0.228
0.413MetMet: 0.413 ± 0.095
1.096MetAsn: 1.096 ± 0.169
1.078MetPro: 1.078 ± 0.159
1.043MetGln: 1.043 ± 0.168
0.971MetArg: 0.971 ± 0.13
1.582MetSer: 1.582 ± 0.246
1.762MetThr: 1.762 ± 0.239
1.025MetVal: 1.025 ± 0.14
0.27MetTrp: 0.27 ± 0.061
0.647MetTyr: 0.647 ± 0.128
0.0MetXaa: 0.0 ± 0.0
Asn
3.667AsnAla: 3.667 ± 0.275
0.467AsnCys: 0.467 ± 0.094
3.361AsnAsp: 3.361 ± 0.241
3.038AsnGlu: 3.038 ± 0.227
2.678AsnPhe: 2.678 ± 0.215
4.781AsnGly: 4.781 ± 0.53
0.827AsnHis: 0.827 ± 0.133
3.99AsnIle: 3.99 ± 0.327
3.128AsnLys: 3.128 ± 0.294
4.584AsnLeu: 4.584 ± 0.308
0.935AsnMet: 0.935 ± 0.14
3.721AsnAsn: 3.721 ± 0.328
3.146AsnPro: 3.146 ± 0.252
1.923AsnGln: 1.923 ± 0.176
2.534AsnArg: 2.534 ± 0.203
3.829AsnSer: 3.829 ± 0.29
3.631AsnThr: 3.631 ± 0.415
4.332AsnVal: 4.332 ± 0.267
0.719AsnTrp: 0.719 ± 0.132
2.534AsnTyr: 2.534 ± 0.21
0.0AsnXaa: 0.0 ± 0.0
Pro
3.092ProAla: 3.092 ± 0.279
0.359ProCys: 0.359 ± 0.126
2.894ProAsp: 2.894 ± 0.307
2.57ProGlu: 2.57 ± 0.282
1.797ProPhe: 1.797 ± 0.178
3.307ProGly: 3.307 ± 0.282
0.521ProHis: 0.521 ± 0.09
2.265ProIle: 2.265 ± 0.22
2.247ProLys: 2.247 ± 0.264
2.301ProLeu: 2.301 ± 0.211
0.755ProMet: 0.755 ± 0.139
2.319ProAsn: 2.319 ± 0.221
1.923ProPro: 1.923 ± 0.256
1.42ProGln: 1.42 ± 0.145
1.492ProArg: 1.492 ± 0.133
3.056ProSer: 3.056 ± 0.214
3.002ProThr: 3.002 ± 0.226
2.66ProVal: 2.66 ± 0.255
0.521ProTrp: 0.521 ± 0.107
1.636ProTyr: 1.636 ± 0.193
0.0ProXaa: 0.0 ± 0.0
Gln
2.193GlnAla: 2.193 ± 0.218
0.324GlnCys: 0.324 ± 0.084
2.103GlnAsp: 2.103 ± 0.17
2.391GlnGlu: 2.391 ± 0.18
1.887GlnPhe: 1.887 ± 0.178
2.373GlnGly: 2.373 ± 0.211
0.665GlnHis: 0.665 ± 0.137
2.768GlnIle: 2.768 ± 0.262
2.337GlnLys: 2.337 ± 0.259
3.469GlnLeu: 3.469 ± 0.245
0.791GlnMet: 0.791 ± 0.139
1.797GlnAsn: 1.797 ± 0.166
1.078GlnPro: 1.078 ± 0.12
1.618GlnGln: 1.618 ± 0.196
1.959GlnArg: 1.959 ± 0.17
2.355GlnSer: 2.355 ± 0.202
2.445GlnThr: 2.445 ± 0.198
2.534GlnVal: 2.534 ± 0.216
0.377GlnTrp: 0.377 ± 0.075
1.797GlnTyr: 1.797 ± 0.194
0.0GlnXaa: 0.0 ± 0.0
Arg
3.092ArgAla: 3.092 ± 0.266
0.503ArgCys: 0.503 ± 0.084
2.463ArgAsp: 2.463 ± 0.192
2.337ArgGlu: 2.337 ± 0.272
1.833ArgPhe: 1.833 ± 0.165
2.786ArgGly: 2.786 ± 0.225
0.647ArgHis: 0.647 ± 0.113
3.074ArgIle: 3.074 ± 0.256
2.642ArgLys: 2.642 ± 0.283
3.487ArgLeu: 3.487 ± 0.224
1.15ArgMet: 1.15 ± 0.149
2.103ArgAsn: 2.103 ± 0.187
1.582ArgPro: 1.582 ± 0.192
1.636ArgGln: 1.636 ± 0.17
1.995ArgArg: 1.995 ± 0.296
1.959ArgSer: 1.959 ± 0.193
2.391ArgThr: 2.391 ± 0.26
3.361ArgVal: 3.361 ± 0.302
0.467ArgTrp: 0.467 ± 0.097
2.157ArgTyr: 2.157 ± 0.19
0.0ArgXaa: 0.0 ± 0.0
Ser
5.303SerAla: 5.303 ± 0.372
0.647SerCys: 0.647 ± 0.125
4.404SerAsp: 4.404 ± 0.388
3.577SerGlu: 3.577 ± 0.259
3.361SerPhe: 3.361 ± 0.256
6.309SerGly: 6.309 ± 0.574
0.917SerHis: 0.917 ± 0.141
4.602SerIle: 4.602 ± 0.409
3.451SerLys: 3.451 ± 0.286
4.835SerLeu: 4.835 ± 0.274
1.312SerMet: 1.312 ± 0.186
3.361SerAsn: 3.361 ± 0.311
2.588SerPro: 2.588 ± 0.253
2.427SerGln: 2.427 ± 0.243
2.373SerArg: 2.373 ± 0.187
4.835SerSer: 4.835 ± 0.332
5.195SerThr: 5.195 ± 0.389
4.368SerVal: 4.368 ± 0.374
0.611SerTrp: 0.611 ± 0.123
2.822SerTyr: 2.822 ± 0.213
0.0SerXaa: 0.0 ± 0.0
Thr
5.788ThrAla: 5.788 ± 0.489
0.377ThrCys: 0.377 ± 0.076
4.368ThrAsp: 4.368 ± 0.34
3.829ThrGlu: 3.829 ± 0.263
3.559ThrPhe: 3.559 ± 0.299
6.363ThrGly: 6.363 ± 0.631
0.917ThrHis: 0.917 ± 0.148
4.943ThrIle: 4.943 ± 0.438
3.559ThrLys: 3.559 ± 0.323
6.201ThrLeu: 6.201 ± 0.42
1.15ThrMet: 1.15 ± 0.175
4.26ThrAsn: 4.26 ± 0.381
3.361ThrPro: 3.361 ± 0.237
2.606ThrGln: 2.606 ± 0.184
2.768ThrArg: 2.768 ± 0.234
4.871ThrSer: 4.871 ± 0.451
5.896ThrThr: 5.896 ± 0.619
5.41ThrVal: 5.41 ± 0.522
0.791ThrTrp: 0.791 ± 0.112
2.984ThrTyr: 2.984 ± 0.263
0.0ThrXaa: 0.0 ± 0.0
Val
4.925ValAla: 4.925 ± 0.292
0.449ValCys: 0.449 ± 0.11
5.608ValAsp: 5.608 ± 0.45
4.386ValGlu: 4.386 ± 0.246
2.75ValPhe: 2.75 ± 0.22
4.925ValGly: 4.925 ± 0.456
0.629ValHis: 0.629 ± 0.1
4.152ValIle: 4.152 ± 0.331
3.972ValLys: 3.972 ± 0.3
3.936ValLeu: 3.936 ± 0.293
1.384ValMet: 1.384 ± 0.204
4.17ValAsn: 4.17 ± 0.331
2.606ValPro: 2.606 ± 0.206
2.175ValGln: 2.175 ± 0.162
2.768ValArg: 2.768 ± 0.176
4.745ValSer: 4.745 ± 0.256
5.914ValThr: 5.914 ± 0.491
4.727ValVal: 4.727 ± 0.405
0.701ValTrp: 0.701 ± 0.084
2.588ValTyr: 2.588 ± 0.22
0.0ValXaa: 0.0 ± 0.0
Trp
0.845TrpAla: 0.845 ± 0.143
0.162TrpCys: 0.162 ± 0.056
0.809TrpAsp: 0.809 ± 0.123
0.809TrpGlu: 0.809 ± 0.179
0.503TrpPhe: 0.503 ± 0.106
0.611TrpGly: 0.611 ± 0.099
0.324TrpHis: 0.324 ± 0.083
0.755TrpIle: 0.755 ± 0.107
0.755TrpLys: 0.755 ± 0.133
0.701TrpLeu: 0.701 ± 0.133
0.395TrpMet: 0.395 ± 0.084
0.791TrpAsn: 0.791 ± 0.13
0.234TrpPro: 0.234 ± 0.065
0.395TrpGln: 0.395 ± 0.077
0.485TrpArg: 0.485 ± 0.13
0.791TrpSer: 0.791 ± 0.109
0.827TrpThr: 0.827 ± 0.11
0.791TrpVal: 0.791 ± 0.11
0.126TrpTrp: 0.126 ± 0.047
0.539TrpTyr: 0.539 ± 0.123
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.624TyrAla: 2.624 ± 0.22
0.539TyrCys: 0.539 ± 0.111
3.451TyrAsp: 3.451 ± 0.248
2.445TyrGlu: 2.445 ± 0.249
1.69TyrPhe: 1.69 ± 0.183
2.534TyrGly: 2.534 ± 0.209
0.845TyrHis: 0.845 ± 0.18
2.84TyrIle: 2.84 ± 0.267
2.373TyrLys: 2.373 ± 0.226
3.182TyrLeu: 3.182 ± 0.24
0.989TyrMet: 0.989 ± 0.148
2.804TyrAsn: 2.804 ± 0.269
1.618TyrPro: 1.618 ± 0.188
1.887TyrGln: 1.887 ± 0.164
2.498TyrArg: 2.498 ± 0.232
2.48TyrSer: 2.48 ± 0.216
2.858TyrThr: 2.858 ± 0.344
2.966TyrVal: 2.966 ± 0.265
0.377TyrTrp: 0.377 ± 0.088
1.887TyrTyr: 1.887 ± 0.224
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 228 proteins (55635 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski