Mycobacterium phage LastJedi

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 94 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N0A5I0|A0A6N0A5I0_9CAUD Uncharacterized protein OS=Mycobacterium phage LastJedi OX=2707031 GN=28 PE=4 SV=1
MM1 pKa = 7.71SDD3 pKa = 5.03CLLCDD8 pKa = 3.92HH9 pKa = 7.15PRR11 pKa = 11.84STHH14 pKa = 4.44TPEE17 pKa = 3.71CRR19 pKa = 11.84VRR21 pKa = 11.84MGIDD25 pKa = 3.58PDD27 pKa = 4.04DD28 pKa = 3.79MSVYY32 pKa = 8.7TACLCPGWEE41 pKa = 4.2GTKK44 pKa = 10.64DD45 pKa = 3.58GEE47 pKa = 4.15EE48 pKa = 4.21DD49 pKa = 3.16

Molecular weight: 5.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N0A5G4|A0A6N0A5G4_9CAUD Uncharacterized protein OS=Mycobacterium phage LastJedi OX=2707031 GN=32 PE=4 SV=1
MM1 pKa = 7.43TFFSGRR7 pKa = 11.84RR8 pKa = 11.84GSARR12 pKa = 11.84HH13 pKa = 5.13GKK15 pKa = 8.78GVGMARR21 pKa = 11.84RR22 pKa = 11.84GMARR26 pKa = 11.84LGTTWQAWLGMARR39 pKa = 11.84LGRR42 pKa = 11.84AWLGAARR49 pKa = 11.84HH50 pKa = 5.89GRR52 pKa = 11.84HH53 pKa = 6.33DD54 pKa = 3.38MAGAARR60 pKa = 11.84RR61 pKa = 11.84GG62 pKa = 3.45

Molecular weight: 6.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

94

0

94

17574

30

1183

187.0

20.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.544 ± 0.484

1.275 ± 0.166

6.942 ± 0.19

6.094 ± 0.298

2.936 ± 0.178

8.245 ± 0.453

2.356 ± 0.176

4.364 ± 0.196

3.408 ± 0.197

7.642 ± 0.222

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.293 ± 0.13

3.135 ± 0.166

6.094 ± 0.219

3.534 ± 0.149

7.198 ± 0.329

5.377 ± 0.186

6.584 ± 0.275

7.266 ± 0.296

2.253 ± 0.141

2.458 ± 0.151
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski