Avon-Heathcote Estuary associated circular virus 9

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 8.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5I9M0|A0A0C5I9M0_9CIRC Putative capsid protein OS=Avon-Heathcote Estuary associated circular virus 9 OX=1618260 PE=4 SV=1
MM1 pKa = 7.43SDD3 pKa = 2.77KK4 pKa = 10.71HH5 pKa = 6.91RR6 pKa = 11.84SYY8 pKa = 11.73SFTLNNYY15 pKa = 8.83TDD17 pKa = 4.59DD18 pKa = 3.81EE19 pKa = 4.77VFALTSMCEE28 pKa = 3.93PINRR32 pKa = 11.84GVKK35 pKa = 9.5YY36 pKa = 10.47LIFGKK41 pKa = 10.28EE42 pKa = 3.68IAPTTGTPHH51 pKa = 6.38LQGCIIFTSPRR62 pKa = 11.84SFKK65 pKa = 10.68ALKK68 pKa = 10.31KK69 pKa = 9.68VVPFNRR75 pKa = 11.84ANYY78 pKa = 9.35KK79 pKa = 8.72PTISEE84 pKa = 4.18PGSARR89 pKa = 11.84YY90 pKa = 8.81CKK92 pKa = 10.08KK93 pKa = 10.77DD94 pKa = 3.01GDD96 pKa = 4.02VYY98 pKa = 11.17EE99 pKa = 4.81YY100 pKa = 8.71GTPPKK105 pKa = 9.82QGKK108 pKa = 9.82RR109 pKa = 11.84SDD111 pKa = 3.44IEE113 pKa = 3.98NVRR116 pKa = 11.84DD117 pKa = 3.45ILTSGGNIRR126 pKa = 11.84DD127 pKa = 3.92VVSTATSIQSVRR139 pKa = 11.84MAEE142 pKa = 3.52IHH144 pKa = 6.58LKK146 pKa = 10.43YY147 pKa = 10.47FEE149 pKa = 5.16KK150 pKa = 10.46KK151 pKa = 10.26RR152 pKa = 11.84NWKK155 pKa = 8.23PTVRR159 pKa = 11.84WYY161 pKa = 11.09YY162 pKa = 10.89GATGTGKK169 pKa = 10.54SKK171 pKa = 9.23TAYY174 pKa = 9.51EE175 pKa = 4.39EE176 pKa = 4.47CDD178 pKa = 3.14EE179 pKa = 4.58HH180 pKa = 8.5DD181 pKa = 4.74PYY183 pKa = 11.96VAMSTGKK190 pKa = 9.42WFEE193 pKa = 4.36GYY195 pKa = 9.4DD196 pKa = 3.31AHH198 pKa = 5.75THH200 pKa = 6.15VIIDD204 pKa = 3.88DD205 pKa = 3.62MRR207 pKa = 11.84KK208 pKa = 10.42DD209 pKa = 3.52FMKK212 pKa = 10.43FHH214 pKa = 6.98EE215 pKa = 5.0LLRR218 pKa = 11.84MLDD221 pKa = 3.49RR222 pKa = 11.84YY223 pKa = 11.27AFMVEE228 pKa = 4.26CKK230 pKa = 10.41GGSRR234 pKa = 11.84QFLATDD240 pKa = 3.82IYY242 pKa = 10.24ITSCYY247 pKa = 10.54APDD250 pKa = 3.28EE251 pKa = 4.3MFEE254 pKa = 4.09SRR256 pKa = 11.84EE257 pKa = 4.1DD258 pKa = 3.51VNQLIRR264 pKa = 11.84RR265 pKa = 11.84LDD267 pKa = 3.96EE268 pKa = 3.71IRR270 pKa = 11.84KK271 pKa = 9.53FEE273 pKa = 3.99

Molecular weight: 31.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5I9M0|A0A0C5I9M0_9CIRC Putative capsid protein OS=Avon-Heathcote Estuary associated circular virus 9 OX=1618260 PE=4 SV=1
MM1 pKa = 7.79PYY3 pKa = 9.97VRR5 pKa = 11.84KK6 pKa = 9.14PRR8 pKa = 11.84STMRR12 pKa = 11.84RR13 pKa = 11.84PYY15 pKa = 10.39KK16 pKa = 10.09KK17 pKa = 8.33RR18 pKa = 11.84TMRR21 pKa = 11.84RR22 pKa = 11.84KK23 pKa = 7.82PTASSMYY30 pKa = 9.68NIARR34 pKa = 11.84KK35 pKa = 8.36VLRR38 pKa = 11.84SNVEE42 pKa = 3.85TKK44 pKa = 10.79SRR46 pKa = 11.84ITSYY50 pKa = 9.38STSGMILSHH59 pKa = 5.76NVPRR63 pKa = 11.84LVASQLLQTSQGTDD77 pKa = 2.8SGATSGNRR85 pKa = 11.84VGISVQPVGIKK96 pKa = 10.29LYY98 pKa = 10.53IEE100 pKa = 4.55INQEE104 pKa = 3.51QPAAATGLLNGDD116 pKa = 2.92IWVKK120 pKa = 9.68FWVCSTHH127 pKa = 6.21HH128 pKa = 6.72SNVNTSNDD136 pKa = 3.44FLRR139 pKa = 11.84LISSNTMLAPVQRR152 pKa = 11.84RR153 pKa = 11.84THH155 pKa = 4.82NVIRR159 pKa = 11.84SFKK162 pKa = 10.68LNLRR166 pKa = 11.84NYY168 pKa = 6.65FTHH171 pKa = 6.62WNAGSAIDD179 pKa = 3.81ATPAFKK185 pKa = 10.23TRR187 pKa = 11.84TIWIPMNKK195 pKa = 8.69IKK197 pKa = 10.58QYY199 pKa = 10.85LYY201 pKa = 11.03EE202 pKa = 5.43NDD204 pKa = 3.21VTDD207 pKa = 3.26NGKK210 pKa = 9.66YY211 pKa = 10.18FDD213 pKa = 4.28YY214 pKa = 10.5CCHH217 pKa = 6.23AVAFSAHH224 pKa = 6.52PSVNTSTALAAVKK237 pKa = 10.55LNTEE241 pKa = 4.56FFFKK245 pKa = 10.82DD246 pKa = 2.96AA247 pKa = 4.54

Molecular weight: 27.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

520

247

273

260.0

29.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.346 ± 0.885

1.731 ± 0.34

5.192 ± 1.285

4.615 ± 1.704

4.615 ± 0.373

5.192 ± 0.752

2.692 ± 0.093

5.962 ± 0.193

7.115 ± 0.686

5.577 ± 0.592

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.077 ± 0.16

5.385 ± 1.518

4.423 ± 0.02

2.5 ± 0.486

7.115 ± 0.113

8.077 ± 1.078

8.077 ± 0.546

5.962 ± 0.606

1.346 ± 0.18

5.0 ± 0.626
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski