Aliifodinibius sp. WN023

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Balneolaeota; Balneolia; Balneolales; Balneolaceae; Aliifodinibius; unclassified Aliifodinibius

Average proteome isoelectric point is 5.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3115 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A2G9N8|A0A2A2G9N8_9BACT Succinate-semialdehyde dehydrogenase OS=Aliifodinibius sp. WN023 OX=2032627 GN=CK503_10455 PE=4 SV=1
MM1 pKa = 7.77KK2 pKa = 10.48SLLKK6 pKa = 10.08ILAMASLALLIAQCEE21 pKa = 4.55VIDD24 pKa = 4.68SNLLEE29 pKa = 5.05DD30 pKa = 4.99PNNPSPEE37 pKa = 4.02DD38 pKa = 3.64VNVDD42 pKa = 3.78FLFNNIQQEE51 pKa = 3.95ARR53 pKa = 11.84NVYY56 pKa = 10.43AGAADD61 pKa = 3.65EE62 pKa = 4.51AGEE65 pKa = 4.24MSRR68 pKa = 11.84MTYY71 pKa = 9.7MFGATYY77 pKa = 11.02ADD79 pKa = 4.5AYY81 pKa = 10.03TPTTFDD87 pKa = 3.11GLYY90 pKa = 9.87SNAYY94 pKa = 9.01ADD96 pKa = 4.5LFIDD100 pKa = 3.97VEE102 pKa = 4.2NLIPIAEE109 pKa = 4.11EE110 pKa = 3.74RR111 pKa = 11.84DD112 pKa = 3.64LTFHH116 pKa = 6.42TGAAKK121 pKa = 10.25VLKK124 pKa = 9.46AYY126 pKa = 10.77AMITLVDD133 pKa = 3.73VYY135 pKa = 11.5GDD137 pKa = 3.78VPLSNALDD145 pKa = 3.46QTNFNPEE152 pKa = 4.14LDD154 pKa = 3.99GGAAVYY160 pKa = 10.59DD161 pKa = 3.92SALVLLDD168 pKa = 4.47DD169 pKa = 6.35AIADD173 pKa = 4.18LEE175 pKa = 4.54TEE177 pKa = 4.09PTVYY181 pKa = 10.2PDD183 pKa = 3.04NDD185 pKa = 3.43LYY187 pKa = 11.48YY188 pKa = 11.14GSFEE192 pKa = 4.4DD193 pKa = 6.31RR194 pKa = 11.84EE195 pKa = 4.4DD196 pKa = 5.0QVDD199 pKa = 3.08AWIRR203 pKa = 11.84AANTIKK209 pKa = 10.76LKK211 pKa = 10.77AYY213 pKa = 10.48LNTDD217 pKa = 3.17NASAIQSLVDD227 pKa = 3.48EE228 pKa = 5.17GMLITTSTNNFTYY241 pKa = 10.64QFGTNSTNPDD251 pKa = 2.81SRR253 pKa = 11.84HH254 pKa = 5.71PSFSNNYY261 pKa = 8.48VDD263 pKa = 4.0VANDD267 pKa = 3.59YY268 pKa = 11.02MSVNYY273 pKa = 10.38LNMMLNDD280 pKa = 4.46KK281 pKa = 11.12GDD283 pKa = 3.53VDD285 pKa = 4.29PRR287 pKa = 11.84TPYY290 pKa = 10.25YY291 pKa = 10.38FYY293 pKa = 10.82RR294 pKa = 11.84QTTSDD299 pKa = 3.63PTDD302 pKa = 3.25VNLNTCISAFKK313 pKa = 10.2PNHH316 pKa = 5.77FQQNDD321 pKa = 3.83PFCLLGNGYY330 pKa = 9.07WGRR333 pKa = 11.84DD334 pKa = 3.36HH335 pKa = 7.27LIDD338 pKa = 5.9DD339 pKa = 6.24GIPPDD344 pKa = 3.89GDD346 pKa = 3.38LRR348 pKa = 11.84TTFGVYY354 pKa = 9.62PVGGAFDD361 pKa = 3.73TGQEE365 pKa = 4.04EE366 pKa = 4.82GTEE369 pKa = 3.98EE370 pKa = 5.05DD371 pKa = 3.88MGLQGAGFDD380 pKa = 4.75PILMSSFTHH389 pKa = 6.84FMLAEE394 pKa = 3.71YY395 pKa = 9.93EE396 pKa = 4.06YY397 pKa = 10.9RR398 pKa = 11.84LNGNAGAARR407 pKa = 11.84NHH409 pKa = 6.9LEE411 pKa = 3.88TAMTQSLEE419 pKa = 4.22TVRR422 pKa = 11.84ASAGTLDD429 pKa = 5.14DD430 pKa = 5.43DD431 pKa = 4.59EE432 pKa = 4.91QEE434 pKa = 4.19EE435 pKa = 4.34MSDD438 pKa = 3.65NDD440 pKa = 2.91ITNYY444 pKa = 9.53VDD446 pKa = 3.88NVLNNRR452 pKa = 11.84YY453 pKa = 9.9DD454 pKa = 3.47DD455 pKa = 3.96AGRR458 pKa = 11.84DD459 pKa = 3.72EE460 pKa = 4.95LRR462 pKa = 11.84VISKK466 pKa = 9.7EE467 pKa = 3.88FYY469 pKa = 10.34LALWTNGLEE478 pKa = 4.62AYY480 pKa = 11.33NMMRR484 pKa = 11.84RR485 pKa = 11.84TGYY488 pKa = 9.64PNKK491 pKa = 9.84QDD493 pKa = 3.44NLQPARR499 pKa = 11.84SPNPGSWYY507 pKa = 10.82NSFLYY512 pKa = 8.83PASMVEE518 pKa = 4.32RR519 pKa = 11.84NNSVSQKK526 pKa = 10.46DD527 pKa = 3.37RR528 pKa = 11.84SEE530 pKa = 4.19TVFWDD535 pKa = 3.49DD536 pKa = 2.72GTYY539 pKa = 11.15NFDD542 pKa = 3.36FF543 pKa = 5.06

Molecular weight: 60.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A2G817|A0A2A2G817_9BACT SAM-dependent methyltransferase OS=Aliifodinibius sp. WN023 OX=2032627 GN=CK503_13805 PE=4 SV=1
MM1 pKa = 7.78QIRR4 pKa = 11.84QPILYY9 pKa = 9.28GLVFILVLILVLVAIGGGLFNQSARR34 pKa = 11.84WPVQWQHH41 pKa = 5.3QAFNSLCHH49 pKa = 5.86QMANRR54 pKa = 11.84SFWINGQPMAVCSRR68 pKa = 11.84CIGVYY73 pKa = 10.19SGFALGWMLLPMLSLIRR90 pKa = 11.84ITRR93 pKa = 11.84LAHH96 pKa = 5.34IKK98 pKa = 10.11KK99 pKa = 9.17VLVVILLFNFIDD111 pKa = 3.81AAGNLLGLWQNTLFSRR127 pKa = 11.84AILGGMLGSSAALIFVGDD145 pKa = 4.51FFHH148 pKa = 7.66HH149 pKa = 6.14NN150 pKa = 3.3

Molecular weight: 16.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3115

0

3115

1052852

25

2870

338.0

38.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.63 ± 0.042

0.607 ± 0.011

6.369 ± 0.04

7.697 ± 0.055

4.503 ± 0.034

6.898 ± 0.035

1.959 ± 0.02

7.092 ± 0.047

5.991 ± 0.052

9.159 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.41 ± 0.023

5.028 ± 0.037

3.81 ± 0.022

4.098 ± 0.032

4.373 ± 0.03

6.7 ± 0.04

5.471 ± 0.032

6.327 ± 0.036

1.209 ± 0.017

3.667 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski