Clunio marinus

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Diptera; Nematocera; Culicomorpha; Chironomoidea; Chironomidae; Orthocladiinae; Cl

Average proteome isoelectric point is 7.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 22544 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J1I037|A0A1J1I037_9DIPT CLUMA_CG005793 isoform A OS=Clunio marinus OX=568069 GN=CLUMA_CG005793 PE=4 SV=1
MM1 pKa = 7.52KK2 pKa = 10.27LLHH5 pKa = 6.65LFLVVIIAEE14 pKa = 4.34EE15 pKa = 4.08AFSGDD20 pKa = 3.86GNSCSSPPLSFSPSDD35 pKa = 3.36IINRR39 pKa = 11.84LDD41 pKa = 3.83NIKK44 pKa = 10.86DD45 pKa = 4.15FIGDD49 pKa = 3.59AVSEE53 pKa = 4.0YY54 pKa = 11.29LPFFEE59 pKa = 5.71GADD62 pKa = 3.4VDD64 pKa = 4.81FGGTNHH70 pKa = 6.49EE71 pKa = 4.26KK72 pKa = 10.77SEE74 pKa = 4.23NAVSLLIEE82 pKa = 4.34LQTVLSNNGIPSSVQDD98 pKa = 4.41FFPLIDD104 pKa = 4.93DD105 pKa = 5.18IIYY108 pKa = 7.51NTQSTGIQKK117 pKa = 10.64KK118 pKa = 8.63IDD120 pKa = 4.04EE121 pKa = 5.25IIASVDD127 pKa = 3.02AASQILYY134 pKa = 10.32AVLGTIIGQIATEE147 pKa = 4.39TNSSLDD153 pKa = 3.6YY154 pKa = 10.94LSDD157 pKa = 3.9VMTQQIDD164 pKa = 3.55TADD167 pKa = 3.73YY168 pKa = 9.23PISCIAEE175 pKa = 3.97ALIQLYY181 pKa = 9.37QFMFNTYY188 pKa = 7.38EE189 pKa = 4.17TIEE192 pKa = 4.15NAAIAAKK199 pKa = 10.51ASMEE203 pKa = 3.91AMPP206 pKa = 5.42

Molecular weight: 22.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J1IDF5|A0A1J1IDF5_9DIPT CLUMA_CG010414 isoform A OS=Clunio marinus OX=568069 GN=CLUMA_CG010414 PE=4 SV=1
MM1 pKa = 6.72NTSTRR6 pKa = 11.84LTLNDD11 pKa = 3.74RR12 pKa = 11.84FSMIKK17 pKa = 9.67QGNGVLPKK25 pKa = 8.84QQRR28 pKa = 11.84GRR30 pKa = 11.84SRR32 pKa = 11.84SRR34 pKa = 11.84SQTRR38 pKa = 11.84RR39 pKa = 11.84PSRR42 pKa = 11.84QLSQGGPRR50 pKa = 11.84KK51 pKa = 9.6NRR53 pKa = 11.84QLLDD57 pKa = 3.52QLEE60 pKa = 4.6KK61 pKa = 8.35QHH63 pKa = 6.03KK64 pKa = 8.75MRR66 pKa = 11.84MAMKK70 pKa = 10.05LKK72 pKa = 9.92NKK74 pKa = 10.23SIMRR78 pKa = 11.84SRR80 pKa = 11.84GRR82 pKa = 11.84VQSQNRR88 pKa = 11.84RR89 pKa = 11.84RR90 pKa = 11.84PANSLIATIKK100 pKa = 10.41RR101 pKa = 11.84KK102 pKa = 8.28MVEE105 pKa = 4.11DD106 pKa = 3.42SLKK109 pKa = 10.67RR110 pKa = 11.84GSNLTTFVPLNDD122 pKa = 3.51GPRR125 pKa = 11.84RR126 pKa = 11.84NFRR129 pKa = 11.84GRR131 pKa = 11.84SRR133 pKa = 11.84SRR135 pKa = 11.84SQSRR139 pKa = 11.84NRR141 pKa = 11.84NQQRR145 pKa = 11.84SQSRR149 pKa = 11.84NRR151 pKa = 11.84NQQQQQGSVIDD162 pKa = 3.83RR163 pKa = 11.84LGVRR167 pKa = 11.84QPLKK171 pKa = 10.34RR172 pKa = 11.84MRR174 pKa = 11.84RR175 pKa = 11.84GPSNGGGQQQRR186 pKa = 11.84SRR188 pKa = 11.84SRR190 pKa = 11.84VRR192 pKa = 11.84LNRR195 pKa = 11.84NNFNNNNVNNNNVNNRR211 pKa = 11.84NVNNRR216 pKa = 11.84NVNNRR221 pKa = 11.84NVNNQGSVRR230 pKa = 11.84RR231 pKa = 11.84SNLLKK236 pKa = 10.51SDD238 pKa = 4.13LNDD241 pKa = 3.36GFALLNQLRR250 pKa = 11.84RR251 pKa = 11.84FALNLPKK258 pKa = 10.47NRR260 pKa = 11.84SNQLNRR266 pKa = 11.84PLRR269 pKa = 11.84GRR271 pKa = 11.84IVKK274 pKa = 10.29RR275 pKa = 11.84NQNRR279 pKa = 11.84QTGNGAGVGGGRR291 pKa = 11.84IARR294 pKa = 11.84NIRR297 pKa = 11.84QNPNMADD304 pKa = 3.64GNRR307 pKa = 11.84QRR309 pKa = 11.84GKK311 pKa = 9.94IIKK314 pKa = 9.99RR315 pKa = 11.84SAQAQTRR322 pKa = 11.84GRR324 pKa = 11.84AVNRR328 pKa = 11.84KK329 pKa = 7.61QANQRR334 pKa = 11.84NGQGQAGRR342 pKa = 11.84RR343 pKa = 11.84GRR345 pKa = 11.84SRR347 pKa = 11.84TRR349 pKa = 11.84RR350 pKa = 11.84GVSNNNNNSRR360 pKa = 11.84GIIQNLKK367 pKa = 9.09QGHH370 pKa = 5.77KK371 pKa = 10.06VVEE374 pKa = 4.46VSS376 pKa = 3.19

Molecular weight: 43.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21611

933

22544

8391700

14

15766

372.2

42.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.055 ± 0.014

1.982 ± 0.023

5.399 ± 0.016

6.783 ± 0.028

4.662 ± 0.021

4.677 ± 0.023

2.494 ± 0.011

6.716 ± 0.018

7.439 ± 0.026

8.97 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.496 ± 0.008

6.212 ± 0.019

4.334 ± 0.021

4.3 ± 0.019

4.781 ± 0.016

8.494 ± 0.03

5.634 ± 0.02

5.533 ± 0.014

0.951 ± 0.006

3.088 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski