Rhodococcus phage Toil

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes; Kalamavirales; Tectiviridae; Epsilontectivirus; Rhodococcus virus Toil

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 35 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6DXW4|A0A1W6DXW4_9VIRU Uncharacterized protein OS=Rhodococcus phage Toil OX=1975614 GN=Toil_gp13 PE=4 SV=1
MM1 pKa = 7.56NEE3 pKa = 3.7NHH5 pKa = 6.5SNAVDD10 pKa = 4.23LSQTVSAKK18 pKa = 9.97HH19 pKa = 5.21PNIHH23 pKa = 6.04RR24 pKa = 11.84VGMANAVVYY33 pKa = 8.31TYY35 pKa = 11.33AVTSTMVLEE44 pKa = 5.69GYY46 pKa = 10.54DD47 pKa = 3.45PTDD50 pKa = 3.53FDD52 pKa = 3.82MLDD55 pKa = 4.12AIEE58 pKa = 4.13EE59 pKa = 4.28MILAGVNFMNLTDD72 pKa = 4.05RR73 pKa = 11.84DD74 pKa = 3.25EE75 pKa = 4.13WDD77 pKa = 3.15IVEE80 pKa = 4.99AIIMQNAHH88 pKa = 6.02PHH90 pKa = 5.33LL91 pKa = 4.61

Molecular weight: 10.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6DXS2|A0A1W6DXS2_9VIRU Putative endolysin OS=Rhodococcus phage Toil OX=1975614 GN=Toil_gp31 PE=4 SV=1
MM1 pKa = 7.42TEE3 pKa = 4.43AGSATDD9 pKa = 3.72NEE11 pKa = 4.72VLVSHH16 pKa = 6.86LRR18 pKa = 11.84DD19 pKa = 3.41ILGHH23 pKa = 5.32TGRR26 pKa = 11.84ATATRR31 pKa = 11.84SARR34 pKa = 11.84TRR36 pKa = 11.84SGVVDD41 pKa = 4.94DD42 pKa = 3.93YY43 pKa = 9.46TTRR46 pKa = 11.84TVPDD50 pKa = 3.93LLARR54 pKa = 11.84LLHH57 pKa = 5.73VARR60 pKa = 11.84IQQGIRR66 pKa = 11.84QQ67 pKa = 3.67

Molecular weight: 7.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

35

0

35

5491

38

596

156.9

17.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.925 ± 0.647

0.801 ± 0.193

5.718 ± 0.402

4.899 ± 0.509

3.733 ± 0.238

8.669 ± 0.683

1.985 ± 0.31

4.972 ± 0.305

3.515 ± 0.336

7.503 ± 0.386

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.513 ± 0.26

4.753 ± 0.496

5.099 ± 0.432

4.498 ± 0.311

5.026 ± 0.491

6.574 ± 0.393

7.175 ± 0.428

7.54 ± 0.433

1.821 ± 0.221

3.278 ± 0.307
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski