Mycobacterium phage Bromden

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bronvirus; unclassified Bronvirus

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 118 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345MBP3|A0A345MBP3_9CAUD Uncharacterized protein OS=Mycobacterium phage Bromden OX=2283252 GN=112 PE=4 SV=1
MM1 pKa = 7.51AFDD4 pKa = 3.51EE5 pKa = 4.53AAYY8 pKa = 10.09DD9 pKa = 3.75QAVHH13 pKa = 7.36DD14 pKa = 4.31FAEE17 pKa = 4.38QNGYY21 pKa = 10.62LPVLTAINALSAFTAQYY38 pKa = 10.84LADD41 pKa = 4.16HH42 pKa = 6.57VNDD45 pKa = 3.78

Molecular weight: 4.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345MBH4|A0A345MBH4_9CAUD Immunity repressor OS=Mycobacterium phage Bromden OX=2283252 GN=39 PE=4 SV=1
MM1 pKa = 7.58IAQLSHH7 pKa = 5.55VAAVVPPADD16 pKa = 3.68LALMVLLIAATIASQTVRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84TWRR39 pKa = 11.84IRR41 pKa = 11.84WEE43 pKa = 4.03TGATLTGALMCVALVLLSPNLIALNPFIPDD73 pKa = 3.44SLDD76 pKa = 3.36GGNVYY81 pKa = 9.32PQLMAGHH88 pKa = 6.81IVLMAAYY95 pKa = 7.89ATFLTDD101 pKa = 4.17LAARR105 pKa = 11.84MDD107 pKa = 3.38WTGEE111 pKa = 3.93KK112 pKa = 9.64KK113 pKa = 9.42HH114 pKa = 6.14WYY116 pKa = 6.99IASRR120 pKa = 11.84ISIPSTVAVPTLLGAWVTGDD140 pKa = 3.06QWVVYY145 pKa = 8.08PIVFVGYY152 pKa = 9.6IWLLSNVCWLLWCIRR167 pKa = 11.84TSDD170 pKa = 3.75SRR172 pKa = 11.84SHH174 pKa = 6.43LVVDD178 pKa = 4.33VYY180 pKa = 10.6MIAFALAILAWSSRR194 pKa = 11.84YY195 pKa = 9.0VVSLSLTNNWGWRR208 pKa = 11.84LATIGAILLMLGSAFSWRR226 pKa = 11.84RR227 pKa = 11.84KK228 pKa = 8.37LRR230 pKa = 11.84YY231 pKa = 8.66MKK233 pKa = 10.26AHH235 pKa = 5.47VWRR238 pKa = 11.84TIRR241 pKa = 11.84RR242 pKa = 11.84QKK244 pKa = 9.19PLKK247 pKa = 10.24RR248 pKa = 11.84KK249 pKa = 9.76AKK251 pKa = 10.47APAPKK256 pKa = 10.17QLDD259 pKa = 3.84GAVV262 pKa = 3.17

Molecular weight: 29.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

118

0

118

21344

30

1697

180.9

19.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.275 ± 0.303

1.199 ± 0.192

6.77 ± 0.216

5.585 ± 0.254

3.261 ± 0.106

8.485 ± 0.431

1.851 ± 0.144

3.865 ± 0.142

5.182 ± 0.24

8.574 ± 0.261

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.375 ± 0.115

3.678 ± 0.194

5.299 ± 0.179

3.594 ± 0.189

6.063 ± 0.311

5.641 ± 0.236

5.618 ± 0.232

7.529 ± 0.267

2.221 ± 0.2

2.938 ± 0.167
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski