Amino acid dipepetide frequency for Cytophagaceae bacterium FSY-15

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.091AlaAla: 5.091 ± 0.127
0.657AlaCys: 0.657 ± 0.028
3.502AlaAsp: 3.502 ± 0.082
4.249AlaGlu: 4.249 ± 0.096
3.403AlaPhe: 3.403 ± 0.064
5.184AlaGly: 5.184 ± 0.121
1.206AlaHis: 1.206 ± 0.039
5.451AlaIle: 5.451 ± 0.096
5.284AlaLys: 5.284 ± 0.091
6.342AlaLeu: 6.342 ± 0.108
1.633AlaMet: 1.633 ± 0.05
3.919AlaAsn: 3.919 ± 0.101
2.269AlaPro: 2.269 ± 0.068
2.862AlaGln: 2.862 ± 0.066
2.043AlaArg: 2.043 ± 0.065
4.502AlaSer: 4.502 ± 0.103
3.645AlaThr: 3.645 ± 0.174
3.982AlaVal: 3.982 ± 0.089
0.726AlaTrp: 0.726 ± 0.03
2.483AlaTyr: 2.483 ± 0.062
0.0AlaXaa: 0.0 ± 0.0
Cys
0.486CysAla: 0.486 ± 0.027
0.123CysCys: 0.123 ± 0.013
0.451CysAsp: 0.451 ± 0.032
0.492CysGlu: 0.492 ± 0.026
0.425CysPhe: 0.425 ± 0.022
0.643CysGly: 0.643 ± 0.033
0.231CysHis: 0.231 ± 0.021
0.593CysIle: 0.593 ± 0.025
0.486CysLys: 0.486 ± 0.024
0.781CysLeu: 0.781 ± 0.025
0.186CysMet: 0.186 ± 0.015
0.38CysAsn: 0.38 ± 0.024
0.376CysPro: 0.376 ± 0.024
0.336CysGln: 0.336 ± 0.017
0.235CysArg: 0.235 ± 0.017
0.569CysSer: 0.569 ± 0.038
0.454CysThr: 0.454 ± 0.028
0.447CysVal: 0.447 ± 0.024
0.083CysTrp: 0.083 ± 0.011
0.269CysTyr: 0.269 ± 0.019
0.0CysXaa: 0.0 ± 0.0
Asp
3.64AspAla: 3.64 ± 0.12
0.394AspCys: 0.394 ± 0.028
1.892AspAsp: 1.892 ± 0.059
3.18AspGlu: 3.18 ± 0.074
3.435AspPhe: 3.435 ± 0.066
3.642AspGly: 3.642 ± 0.172
0.815AspHis: 0.815 ± 0.03
3.339AspIle: 3.339 ± 0.072
3.566AspLys: 3.566 ± 0.073
5.598AspLeu: 5.598 ± 0.089
1.046AspMet: 1.046 ± 0.033
2.18AspAsn: 2.18 ± 0.075
2.05AspPro: 2.05 ± 0.053
1.715AspGln: 1.715 ± 0.045
1.8AspArg: 1.8 ± 0.049
3.046AspSer: 3.046 ± 0.077
2.099AspThr: 2.099 ± 0.066
3.136AspVal: 3.136 ± 0.06
0.823AspTrp: 0.823 ± 0.032
2.279AspTyr: 2.279 ± 0.054
0.0AspXaa: 0.0 ± 0.0
Glu
4.345GluAla: 4.345 ± 0.098
0.334GluCys: 0.334 ± 0.021
2.808GluAsp: 2.808 ± 0.065
4.49GluGlu: 4.49 ± 0.091
3.086GluPhe: 3.086 ± 0.066
3.729GluGly: 3.729 ± 0.081
1.062GluHis: 1.062 ± 0.035
5.54GluIle: 5.54 ± 0.092
6.072GluLys: 6.072 ± 0.11
6.132GluLeu: 6.132 ± 0.101
1.64GluMet: 1.64 ± 0.042
4.148GluAsn: 4.148 ± 0.068
1.677GluPro: 1.677 ± 0.051
2.377GluGln: 2.377 ± 0.063
2.148GluArg: 2.148 ± 0.051
3.323GluSer: 3.323 ± 0.063
2.962GluThr: 2.962 ± 0.064
4.104GluVal: 4.104 ± 0.089
0.757GluTrp: 0.757 ± 0.033
2.0GluTyr: 2.0 ± 0.05
0.0GluXaa: 0.0 ± 0.0
Phe
3.417PheAla: 3.417 ± 0.078
0.528PheCys: 0.528 ± 0.025
3.064PheAsp: 3.064 ± 0.064
3.388PheGlu: 3.388 ± 0.083
2.801PhePhe: 2.801 ± 0.088
3.908PheGly: 3.908 ± 0.098
0.938PheHis: 0.938 ± 0.036
3.752PheIle: 3.752 ± 0.082
3.635PheLys: 3.635 ± 0.079
5.308PheLeu: 5.308 ± 0.125
1.103PheMet: 1.103 ± 0.047
2.813PheAsn: 2.813 ± 0.068
2.088PhePro: 2.088 ± 0.064
2.185PheGln: 2.185 ± 0.055
1.692PheArg: 1.692 ± 0.052
4.108PheSer: 4.108 ± 0.094
2.814PheThr: 2.814 ± 0.071
3.235PheVal: 3.235 ± 0.059
0.744PheTrp: 0.744 ± 0.032
2.149PheTyr: 2.149 ± 0.061
0.0PheXaa: 0.0 ± 0.0
Gly
4.685GlyAla: 4.685 ± 0.119
0.565GlyCys: 0.565 ± 0.036
3.43GlyAsp: 3.43 ± 0.086
3.61GlyGlu: 3.61 ± 0.079
4.054GlyPhe: 4.054 ± 0.073
5.113GlyGly: 5.113 ± 0.106
1.213GlyHis: 1.213 ± 0.04
6.096GlyIle: 6.096 ± 0.119
5.908GlyLys: 5.908 ± 0.086
6.766GlyLeu: 6.766 ± 0.094
1.795GlyMet: 1.795 ± 0.056
4.053GlyAsn: 4.053 ± 0.105
1.815GlyPro: 1.815 ± 0.043
2.447GlyGln: 2.447 ± 0.061
2.318GlyArg: 2.318 ± 0.061
4.277GlySer: 4.277 ± 0.105
4.333GlyThr: 4.333 ± 0.193
4.388GlyVal: 4.388 ± 0.082
0.853GlyTrp: 0.853 ± 0.033
2.665GlyTyr: 2.665 ± 0.063
0.0GlyXaa: 0.0 ± 0.0
His
1.091HisAla: 1.091 ± 0.048
0.2HisCys: 0.2 ± 0.015
0.693HisAsp: 0.693 ± 0.031
0.979HisGlu: 0.979 ± 0.031
1.292HisPhe: 1.292 ± 0.038
1.164HisGly: 1.164 ± 0.041
0.474HisHis: 0.474 ± 0.031
1.293HisIle: 1.293 ± 0.045
1.05HisLys: 1.05 ± 0.033
2.047HisLeu: 2.047 ± 0.052
0.273HisMet: 0.273 ± 0.022
0.733HisAsn: 0.733 ± 0.032
1.097HisPro: 1.097 ± 0.037
0.902HisGln: 0.902 ± 0.039
0.676HisArg: 0.676 ± 0.029
1.136HisSer: 1.136 ± 0.041
0.876HisThr: 0.876 ± 0.036
0.978HisVal: 0.978 ± 0.035
0.279HisTrp: 0.279 ± 0.02
0.729HisTyr: 0.729 ± 0.032
0.0HisXaa: 0.0 ± 0.0
Ile
5.332IleAla: 5.332 ± 0.091
0.82IleCys: 0.82 ± 0.034
4.156IleAsp: 4.156 ± 0.074
5.053IleGlu: 5.053 ± 0.083
3.7IlePhe: 3.7 ± 0.095
5.382IleGly: 5.382 ± 0.101
1.452IleHis: 1.452 ± 0.043
5.2IleIle: 5.2 ± 0.097
5.331IleLys: 5.331 ± 0.091
7.246IleLeu: 7.246 ± 0.12
1.288IleMet: 1.288 ± 0.044
3.962IleAsn: 3.962 ± 0.079
3.616IlePro: 3.616 ± 0.082
3.496IleGln: 3.496 ± 0.071
2.702IleArg: 2.702 ± 0.065
5.902IleSer: 5.902 ± 0.084
3.732IleThr: 3.732 ± 0.082
4.219IleVal: 4.219 ± 0.081
0.845IleTrp: 0.845 ± 0.037
2.601IleTyr: 2.601 ± 0.058
0.0IleXaa: 0.0 ± 0.0
Lys
5.03LysAla: 5.03 ± 0.086
0.383LysCys: 0.383 ± 0.024
4.187LysAsp: 4.187 ± 0.073
5.782LysGlu: 5.782 ± 0.099
3.087LysPhe: 3.087 ± 0.067
5.183LysGly: 5.183 ± 0.096
1.222LysHis: 1.222 ± 0.042
6.452LysIle: 6.452 ± 0.106
6.179LysLys: 6.179 ± 0.116
6.502LysLeu: 6.502 ± 0.097
2.05LysMet: 2.05 ± 0.055
5.145LysAsn: 5.145 ± 0.094
2.698LysPro: 2.698 ± 0.057
2.544LysGln: 2.544 ± 0.064
2.457LysArg: 2.457 ± 0.056
5.2LysSer: 5.2 ± 0.091
3.952LysThr: 3.952 ± 0.068
4.686LysVal: 4.686 ± 0.081
0.826LysTrp: 0.826 ± 0.038
2.797LysTyr: 2.797 ± 0.062
0.0LysXaa: 0.0 ± 0.0
Leu
7.032LeuAla: 7.032 ± 0.113
0.692LeuCys: 0.692 ± 0.031
4.967LeuAsp: 4.967 ± 0.106
6.141LeuGlu: 6.141 ± 0.098
5.292LeuPhe: 5.292 ± 0.126
6.969LeuGly: 6.969 ± 0.111
1.725LeuHis: 1.725 ± 0.054
6.983LeuIle: 6.983 ± 0.123
7.592LeuLys: 7.592 ± 0.107
8.927LeuLeu: 8.927 ± 0.147
2.308LeuMet: 2.308 ± 0.064
5.612LeuAsn: 5.612 ± 0.093
4.148LeuPro: 4.148 ± 0.081
3.585LeuGln: 3.585 ± 0.064
3.161LeuArg: 3.161 ± 0.072
6.923LeuSer: 6.923 ± 0.091
4.897LeuThr: 4.897 ± 0.105
5.729LeuVal: 5.729 ± 0.092
0.911LeuTrp: 0.911 ± 0.032
2.936LeuTyr: 2.936 ± 0.071
0.0LeuXaa: 0.0 ± 0.0
Met
1.858MetAla: 1.858 ± 0.06
0.152MetCys: 0.152 ± 0.013
1.216MetAsp: 1.216 ± 0.042
1.535MetGlu: 1.535 ± 0.04
0.829MetPhe: 0.829 ± 0.035
1.846MetGly: 1.846 ± 0.057
0.402MetHis: 0.402 ± 0.024
1.472MetIle: 1.472 ± 0.045
2.196MetLys: 2.196 ± 0.053
1.814MetLeu: 1.814 ± 0.056
0.612MetMet: 0.612 ± 0.033
1.366MetAsn: 1.366 ± 0.043
0.993MetPro: 0.993 ± 0.035
0.843MetGln: 0.843 ± 0.03
0.824MetArg: 0.824 ± 0.031
1.48MetSer: 1.48 ± 0.045
1.018MetThr: 1.018 ± 0.036
1.425MetVal: 1.425 ± 0.041
0.204MetTrp: 0.204 ± 0.018
0.674MetTyr: 0.674 ± 0.031
0.0MetXaa: 0.0 ± 0.0
Asn
3.757AsnAla: 3.757 ± 0.097
0.495AsnCys: 0.495 ± 0.033
2.505AsnAsp: 2.505 ± 0.076
3.196AsnGlu: 3.196 ± 0.066
3.217AsnPhe: 3.217 ± 0.082
3.841AsnGly: 3.841 ± 0.095
1.084AsnHis: 1.084 ± 0.04
3.693AsnIle: 3.693 ± 0.062
3.881AsnLys: 3.881 ± 0.082
5.997AsnLeu: 5.997 ± 0.109
1.171AsnMet: 1.171 ± 0.037
3.021AsnAsn: 3.021 ± 0.088
3.033AsnPro: 3.033 ± 0.062
2.696AsnGln: 2.696 ± 0.054
2.098AsnArg: 2.098 ± 0.048
4.004AsnSer: 4.004 ± 0.091
2.991AsnThr: 2.991 ± 0.123
3.138AsnVal: 3.138 ± 0.083
0.961AsnTrp: 0.961 ± 0.033
2.675AsnTyr: 2.675 ± 0.066
0.0AsnXaa: 0.0 ± 0.0
Pro
2.676ProAla: 2.676 ± 0.08
0.237ProCys: 0.237 ± 0.016
2.08ProAsp: 2.08 ± 0.073
2.803ProGlu: 2.803 ± 0.06
2.012ProPhe: 2.012 ± 0.061
2.445ProGly: 2.445 ± 0.057
0.688ProHis: 0.688 ± 0.03
3.028ProIle: 3.028 ± 0.07
2.832ProLys: 2.832 ± 0.066
3.3ProLeu: 3.3 ± 0.068
0.894ProMet: 0.894 ± 0.034
2.643ProAsn: 2.643 ± 0.063
0.825ProPro: 0.825 ± 0.039
1.34ProGln: 1.34 ± 0.042
0.973ProArg: 0.973 ± 0.036
2.646ProSer: 2.646 ± 0.057
2.222ProThr: 2.222 ± 0.073
2.58ProVal: 2.58 ± 0.073
0.466ProTrp: 0.466 ± 0.026
1.482ProTyr: 1.482 ± 0.044
0.0ProXaa: 0.0 ± 0.0
Gln
2.61GlnAla: 2.61 ± 0.054
0.2GlnCys: 0.2 ± 0.016
1.521GlnAsp: 1.521 ± 0.046
2.486GlnGlu: 2.486 ± 0.059
2.035GlnPhe: 2.035 ± 0.061
2.208GlnGly: 2.208 ± 0.055
0.632GlnHis: 0.632 ± 0.03
3.438GlnIle: 3.438 ± 0.075
3.612GlnLys: 3.612 ± 0.08
3.89GlnLeu: 3.89 ± 0.06
1.06GlnMet: 1.06 ± 0.033
2.529GlnAsn: 2.529 ± 0.056
1.133GlnPro: 1.133 ± 0.035
1.591GlnGln: 1.591 ± 0.044
1.276GlnArg: 1.276 ± 0.042
2.35GlnSer: 2.35 ± 0.065
2.273GlnThr: 2.273 ± 0.052
2.548GlnVal: 2.548 ± 0.059
0.479GlnTrp: 0.479 ± 0.028
1.246GlnTyr: 1.246 ± 0.045
0.0GlnXaa: 0.0 ± 0.0
Arg
2.104ArgAla: 2.104 ± 0.056
0.211ArgCys: 0.211 ± 0.015
1.575ArgAsp: 1.575 ± 0.054
2.134ArgGlu: 2.134 ± 0.059
1.905ArgPhe: 1.905 ± 0.05
2.091ArgGly: 2.091 ± 0.054
0.511ArgHis: 0.511 ± 0.024
2.765ArgIle: 2.765 ± 0.06
2.614ArgLys: 2.614 ± 0.057
3.208ArgLeu: 3.208 ± 0.064
0.924ArgMet: 0.924 ± 0.032
2.007ArgAsn: 2.007 ± 0.044
1.294ArgPro: 1.294 ± 0.044
1.129ArgGln: 1.129 ± 0.035
1.311ArgArg: 1.311 ± 0.055
1.88ArgSer: 1.88 ± 0.047
1.685ArgThr: 1.685 ± 0.051
2.037ArgVal: 2.037 ± 0.052
0.457ArgTrp: 0.457 ± 0.024
1.233ArgTyr: 1.233 ± 0.04
0.0ArgXaa: 0.0 ± 0.0
Ser
4.205SerAla: 4.205 ± 0.089
0.62SerCys: 0.62 ± 0.029
3.227SerAsp: 3.227 ± 0.082
3.776SerGlu: 3.776 ± 0.073
3.955SerPhe: 3.955 ± 0.08
5.022SerGly: 5.022 ± 0.115
1.169SerHis: 1.169 ± 0.042
5.413SerIle: 5.413 ± 0.09
5.2SerLys: 5.2 ± 0.087
6.578SerLeu: 6.578 ± 0.104
1.39SerMet: 1.39 ± 0.042
4.095SerAsn: 4.095 ± 0.09
2.514SerPro: 2.514 ± 0.054
2.469SerGln: 2.469 ± 0.057
1.916SerArg: 1.916 ± 0.053
4.567SerSer: 4.567 ± 0.11
3.94SerThr: 3.94 ± 0.126
3.893SerVal: 3.893 ± 0.077
0.742SerTrp: 0.742 ± 0.032
2.522SerTyr: 2.522 ± 0.055
0.0SerXaa: 0.0 ± 0.0
Thr
3.718ThrAla: 3.718 ± 0.189
0.441ThrCys: 0.441 ± 0.024
2.712ThrAsp: 2.712 ± 0.09
2.74ThrGlu: 2.74 ± 0.068
2.895ThrPhe: 2.895 ± 0.063
4.265ThrGly: 4.265 ± 0.152
0.999ThrHis: 0.999 ± 0.033
4.102ThrIle: 4.102 ± 0.083
3.271ThrLys: 3.271 ± 0.064
5.107ThrLeu: 5.107 ± 0.086
0.918ThrMet: 0.918 ± 0.038
2.937ThrAsn: 2.937 ± 0.101
2.289ThrPro: 2.289 ± 0.07
1.962ThrGln: 1.962 ± 0.052
1.494ThrArg: 1.494 ± 0.041
3.654ThrSer: 3.654 ± 0.133
2.948ThrThr: 2.948 ± 0.166
3.162ThrVal: 3.162 ± 0.132
0.65ThrTrp: 0.65 ± 0.028
2.281ThrTyr: 2.281 ± 0.081
0.0ThrXaa: 0.0 ± 0.0
Val
4.254ValAla: 4.254 ± 0.083
0.596ValCys: 0.596 ± 0.038
3.276ValAsp: 3.276 ± 0.08
3.914ValGlu: 3.914 ± 0.068
3.107ValPhe: 3.107 ± 0.073
4.334ValGly: 4.334 ± 0.079
1.126ValHis: 1.126 ± 0.034
4.331ValIle: 4.331 ± 0.08
4.358ValLys: 4.358 ± 0.076
5.79ValLeu: 5.79 ± 0.086
1.408ValMet: 1.408 ± 0.046
3.314ValAsn: 3.314 ± 0.073
2.317ValPro: 2.317 ± 0.06
2.19ValGln: 2.19 ± 0.054
2.067ValArg: 2.067 ± 0.057
4.333ValSer: 4.333 ± 0.091
3.101ValThr: 3.101 ± 0.11
3.986ValVal: 3.986 ± 0.08
0.663ValTrp: 0.663 ± 0.031
2.092ValTyr: 2.092 ± 0.05
0.0ValXaa: 0.0 ± 0.0
Trp
0.773TrpAla: 0.773 ± 0.031
0.083TrpCys: 0.083 ± 0.011
0.672TrpAsp: 0.672 ± 0.027
0.812TrpGlu: 0.812 ± 0.033
0.588TrpPhe: 0.588 ± 0.03
0.888TrpGly: 0.888 ± 0.037
0.247TrpHis: 0.247 ± 0.019
0.9TrpIle: 0.9 ± 0.035
0.938TrpLys: 0.938 ± 0.038
1.08TrpLeu: 1.08 ± 0.037
0.378TrpMet: 0.378 ± 0.023
0.771TrpAsn: 0.771 ± 0.032
0.322TrpPro: 0.322 ± 0.022
0.487TrpGln: 0.487 ± 0.022
0.435TrpArg: 0.435 ± 0.022
0.703TrpSer: 0.703 ± 0.029
0.648TrpThr: 0.648 ± 0.03
0.815TrpVal: 0.815 ± 0.031
0.164TrpTrp: 0.164 ± 0.013
0.441TrpTyr: 0.441 ± 0.027
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.329TyrAla: 2.329 ± 0.055
0.314TyrCys: 0.314 ± 0.019
1.741TyrAsp: 1.741 ± 0.052
1.972TyrGlu: 1.972 ± 0.049
2.553TyrPhe: 2.553 ± 0.075
2.528TyrGly: 2.528 ± 0.06
0.767TyrHis: 0.767 ± 0.035
2.195TyrIle: 2.195 ± 0.054
2.36TyrLys: 2.36 ± 0.06
4.118TyrLeu: 4.118 ± 0.085
0.678TyrMet: 0.678 ± 0.031
1.827TyrAsn: 1.827 ± 0.056
1.62TyrPro: 1.62 ± 0.046
1.96TyrGln: 1.96 ± 0.056
1.448TyrArg: 1.448 ± 0.042
2.61TyrSer: 2.61 ± 0.062
1.955TyrThr: 1.955 ± 0.075
2.068TyrVal: 2.068 ± 0.061
0.512TyrTrp: 0.512 ± 0.028
1.577TyrTyr: 1.577 ± 0.051
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2320 proteins (835184 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski