Piscinibacter aquaticus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiales genera incertae sedis; Piscinibacter

Average proteome isoelectric point is 7.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2624 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C6U1Y0|A0A5C6U1Y0_9BURK Urea ABC transporter substrate-binding protein OS=Piscinibacter aquaticus OX=392597 GN=urtA PE=4 SV=1
MM1 pKa = 7.44AVANAGSVNEE11 pKa = 4.09NATLSTTAATGVLANDD27 pKa = 3.77SDD29 pKa = 4.31ADD31 pKa = 3.89AGDD34 pKa = 3.94SVSAVSFGATSGTVGSALAGTYY56 pKa = 7.38GTLTLNSDD64 pKa = 3.31GSYY67 pKa = 9.87TYY69 pKa = 10.71VANRR73 pKa = 11.84PAAEE77 pKa = 4.08ALVGGQVVTEE87 pKa = 4.12SFAYY91 pKa = 8.56TVRR94 pKa = 11.84DD95 pKa = 3.5ASGATATTTLTFTVTGTNDD114 pKa = 3.01MPTITGPLAGAVQEE128 pKa = 5.26DD129 pKa = 4.32GTLTSNGTLLITDD142 pKa = 4.19ADD144 pKa = 3.78AGEE147 pKa = 4.48SGFVAQAGTAGTYY160 pKa = 8.29GTFAITAGGVWTTRR174 pKa = 3.25

Molecular weight: 16.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C6U6X9|A0A5C6U6X9_9BURK ABC transporter permease OS=Piscinibacter aquaticus OX=392597 GN=FSC37_19915 PE=3 SV=1
MM1 pKa = 7.47HH2 pKa = 7.68RR3 pKa = 11.84PPHH6 pKa = 5.72PPPRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84PPPRR17 pKa = 11.84HH18 pKa = 5.9RR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84WNRR25 pKa = 11.84WRR27 pKa = 11.84SVRR30 pKa = 11.84AATIRR35 pKa = 11.84PRR37 pKa = 11.84CAAMPARR44 pKa = 11.84PRR46 pKa = 11.84SSSAARR52 pKa = 11.84RR53 pKa = 11.84SSSTATATSARR64 pKa = 3.97

Molecular weight: 7.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2624

0

2624

661187

24

1208

252.0

27.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.198 ± 0.079

1.154 ± 0.021

5.031 ± 0.034

5.195 ± 0.04

3.166 ± 0.033

8.554 ± 0.05

2.295 ± 0.027

3.772 ± 0.034

2.715 ± 0.051

10.318 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.303 ± 0.027

2.109 ± 0.026

5.777 ± 0.048

3.546 ± 0.031

8.789 ± 0.073

5.467 ± 0.043

4.948 ± 0.047

7.324 ± 0.041

1.55 ± 0.021

1.788 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski