Escherichia phage If1 (Bacteriophage If1)

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; Infulavirus; Escherichia virus If1

Average proteome isoelectric point is 7.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|O80298|G6P_BPIF1 Head virion protein G6P OS=Escherichia phage If1 OX=10868 GN=VI PE=3 SV=1
MM1 pKa = 7.42KK2 pKa = 10.18KK3 pKa = 10.17IIIALFFAPFFTHH16 pKa = 6.46ATTDD20 pKa = 3.68AEE22 pKa = 4.63CLSKK26 pKa = 10.44PAFDD30 pKa = 3.9GTLSNVWKK38 pKa = 10.47EE39 pKa = 3.62GDD41 pKa = 3.17SRR43 pKa = 11.84YY44 pKa = 10.91ANFEE48 pKa = 3.69NCIYY52 pKa = 9.05EE53 pKa = 3.97LSGIGIGYY61 pKa = 10.46DD62 pKa = 3.38NDD64 pKa = 4.18TSCNGHH70 pKa = 5.18WTPVRR75 pKa = 11.84AADD78 pKa = 3.9GSGNGGDD85 pKa = 4.56DD86 pKa = 3.53NSSGGGSNGDD96 pKa = 3.44SGNNSTPDD104 pKa = 3.36TVTPGQTVNLPSDD117 pKa = 4.35LSTLSIPANVVKK129 pKa = 10.47SDD131 pKa = 4.09SIGSQFSLYY140 pKa = 10.11TNASCTMCSGYY151 pKa = 10.73YY152 pKa = 10.23LSNNADD158 pKa = 3.78SIAIANITEE167 pKa = 4.43TVKK170 pKa = 10.99ADD172 pKa = 3.48YY173 pKa = 9.49NQPDD177 pKa = 3.16MWFEE181 pKa = 4.04QTDD184 pKa = 3.28SDD186 pKa = 4.22GNHH189 pKa = 5.51VKK191 pKa = 10.27ILQNSYY197 pKa = 10.73KK198 pKa = 9.99AVSYY202 pKa = 10.6NVEE205 pKa = 4.35SKK207 pKa = 10.86QSDD210 pKa = 4.13VNNPTYY216 pKa = 10.65INYY219 pKa = 10.04SYY221 pKa = 11.1SVNVKK226 pKa = 9.34QVSYY230 pKa = 8.93DD231 pKa = 3.55TSNVCIMNWEE241 pKa = 4.28TFQNKK246 pKa = 8.99CDD248 pKa = 3.52ASRR251 pKa = 11.84AVLITDD257 pKa = 4.33TVTPSYY263 pKa = 10.82SRR265 pKa = 11.84NITIQSNINYY275 pKa = 9.43QGSNGSGGSGGSGGSGNDD293 pKa = 3.78GGGTGNNGNGTGDD306 pKa = 3.51FDD308 pKa = 4.26YY309 pKa = 11.8VKK311 pKa = 10.01MANANKK317 pKa = 10.17DD318 pKa = 3.36ALTEE322 pKa = 4.11SFDD325 pKa = 4.97LSALQADD332 pKa = 4.6TGASLDD338 pKa = 3.71GSVQGTLDD346 pKa = 3.73SLSGFSDD353 pKa = 3.8SIGGLVGNGSAISGEE368 pKa = 4.28FAGSSAAMNAIGEE381 pKa = 4.42GDD383 pKa = 3.95KK384 pKa = 11.47SPLLDD389 pKa = 3.62SLSFLKK395 pKa = 10.87DD396 pKa = 3.23GLFPALPEE404 pKa = 4.6FKK406 pKa = 10.21QCTPFVFAPGKK417 pKa = 8.92EE418 pKa = 3.82YY419 pKa = 11.02EE420 pKa = 4.78FIIEE424 pKa = 4.31CKK426 pKa = 10.2YY427 pKa = 10.49IDD429 pKa = 3.67MFKK432 pKa = 10.95GIFAFILYY440 pKa = 8.97FWTFVTVYY448 pKa = 10.71DD449 pKa = 4.09SFSGILRR456 pKa = 11.84KK457 pKa = 10.09GRR459 pKa = 11.84GG460 pKa = 3.19

Molecular weight: 48.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|O80302|REP_BPIF1 Replication-associated protein G2P OS=Escherichia phage If1 OX=10868 GN=II PE=3 SV=1
MM1 pKa = 7.11WKK3 pKa = 10.27KK4 pKa = 8.54MPEE7 pKa = 4.07YY8 pKa = 10.44IFLQDD13 pKa = 4.06AYY15 pKa = 10.08TDD17 pKa = 3.7NLQQKK22 pKa = 10.34LKK24 pKa = 10.51DD25 pKa = 3.44LHH27 pKa = 5.86TAWKK31 pKa = 10.03RR32 pKa = 11.84CFDD35 pKa = 3.67KK36 pKa = 11.08KK37 pKa = 10.55LAAKK41 pKa = 10.4APVWKK46 pKa = 10.06RR47 pKa = 11.84KK48 pKa = 9.89NEE50 pKa = 3.75GRR52 pKa = 11.84DD53 pKa = 3.1SIRR56 pKa = 11.84FVNFEE61 pKa = 4.56KK62 pKa = 11.03YY63 pKa = 9.96CCLEE67 pKa = 3.61NRR69 pKa = 11.84RR70 pKa = 11.84VKK72 pKa = 10.73LPSGLGWVKK81 pKa = 10.01FRR83 pKa = 11.84QSQRR87 pKa = 11.84VNGKK91 pKa = 9.09IKK93 pKa = 10.68NATISQLAGQWYY105 pKa = 9.51ISFQVEE111 pKa = 3.95IEE113 pKa = 4.18TAEE116 pKa = 4.25PNHH119 pKa = 6.39TSTTIVGLDD128 pKa = 3.14AGVAKK133 pKa = 10.58LATLSDD139 pKa = 3.65GTVFEE144 pKa = 5.1PVNSFQKK151 pKa = 9.96NQKK154 pKa = 9.76KK155 pKa = 9.43LARR158 pKa = 11.84LQRR161 pKa = 11.84QLSRR165 pKa = 11.84KK166 pKa = 9.69VKK168 pKa = 10.59FSNNWQKK175 pKa = 10.65QKK177 pKa = 11.19RR178 pKa = 11.84KK179 pKa = 9.01IQCLHH184 pKa = 5.94SRR186 pKa = 11.84IANIRR191 pKa = 11.84RR192 pKa = 11.84DD193 pKa = 3.89YY194 pKa = 10.14LHH196 pKa = 7.11KK197 pKa = 10.62VTTTVSKK204 pKa = 8.75NHH206 pKa = 5.39AMIVIEE212 pKa = 4.52DD213 pKa = 3.94LKK215 pKa = 10.68VSNMSKK221 pKa = 10.13SAAGTVSQPGRR232 pKa = 11.84NVRR235 pKa = 11.84AKK237 pKa = 10.5SGLNRR242 pKa = 11.84SILDD246 pKa = 3.43QGWYY250 pKa = 9.21EE251 pKa = 3.93MRR253 pKa = 11.84RR254 pKa = 11.84QLEE257 pKa = 4.23YY258 pKa = 10.35KK259 pKa = 9.57QLWRR263 pKa = 11.84GGQVLAVPPAYY274 pKa = 9.4TSQRR278 pKa = 11.84CACCGHH284 pKa = 5.45TAKK287 pKa = 10.5EE288 pKa = 4.1NRR290 pKa = 11.84LSQSQFRR297 pKa = 11.84CQVCGYY303 pKa = 6.91TANADD308 pKa = 3.46VNGARR313 pKa = 11.84NILAAGHH320 pKa = 6.74AVLACGGTMQSDD332 pKa = 3.59RR333 pKa = 11.84PLKK336 pKa = 10.19QEE338 pKa = 3.99PDD340 pKa = 3.51AEE342 pKa = 4.31QSAVTHH348 pKa = 6.62

Molecular weight: 39.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

2

12

2471

32

460

205.9

22.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.365 ± 0.703

1.7 ± 0.341

5.301 ± 0.556

3.642 ± 0.336

4.816 ± 0.391

7.285 ± 0.845

1.538 ± 0.406

6.394 ± 0.469

6.677 ± 0.731

8.175 ± 0.729

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.781 ± 0.146

4.978 ± 0.939

3.885 ± 0.569

3.561 ± 0.729

4.573 ± 0.853

9.51 ± 0.927

6.273 ± 0.634

7.689 ± 0.663

1.255 ± 0.257

3.602 ± 0.454
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski